Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular organisms.Abnormality in apoptosis often leads to severe diseases such as cancers. Better understand...Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular organisms.Abnormality in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in He La cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis.展开更多
Fluorescence imaging is an important branch of bioimaging.It is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of interest.Although the spatial...Fluorescence imaging is an important branch of bioimaging.It is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of interest.Although the spatial resolution limit of optical microscopes is about 200 nm,due to the diffraction limit,with the application of super-resolution fluorescence microscopy technologies this limit has been pushed below 30 nm.This makes it feasible to visualize biological structures in subcellular levels and to monitor subcellular biological processes in real time.However,due to the complexity of the biological structure and components within cells,simultaneous staining and monitoring multiple intracellular components with different coloured fluorophores is often needed during multiplex imaging,to better understand biological processes.Aggregation-induced emission luminogens(AIEgen)and AIEgen based nanoparticles(NPs)have presented many advantages in fluorescence imaging,with strong potential for biological science and nano-medicine.Herein this review,we focus on the advantages of AIEgen and AIEgen NP in cell-based fluorescence imaging,and the latest advances of AIEgens in cell-based multiplex imaging are summarized and discussed.The future perspectives are proposed.展开更多
基金supported by the Hong Kong Branch of Chinese National Engineering Research Centres for Tissue Restoration and Reconstruction
文摘Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular organisms.Abnormality in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in He La cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis.
基金supported by the Australia-China Science and Research Fund Joint Research Centre on Personal Health Technologies, Science and Technology Bureau of Sichuan Province (China) (19YYJC1760)Sichuan Provincial Academician (Expert) Workstation (China) (2018YSGZZ03)Sichuan University of Science and Engineering (China) (2017RCL72)
文摘Fluorescence imaging is an important branch of bioimaging.It is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of interest.Although the spatial resolution limit of optical microscopes is about 200 nm,due to the diffraction limit,with the application of super-resolution fluorescence microscopy technologies this limit has been pushed below 30 nm.This makes it feasible to visualize biological structures in subcellular levels and to monitor subcellular biological processes in real time.However,due to the complexity of the biological structure and components within cells,simultaneous staining and monitoring multiple intracellular components with different coloured fluorophores is often needed during multiplex imaging,to better understand biological processes.Aggregation-induced emission luminogens(AIEgen)and AIEgen based nanoparticles(NPs)have presented many advantages in fluorescence imaging,with strong potential for biological science and nano-medicine.Herein this review,we focus on the advantages of AIEgen and AIEgen NP in cell-based fluorescence imaging,and the latest advances of AIEgens in cell-based multiplex imaging are summarized and discussed.The future perspectives are proposed.
