The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the ...The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the animal flocks and the current trends of antibiotic use in small ruminants. A total of 77 mountainous sheepfolds were investigated representing 10% of all transhumant sheep and goats flocks in the area. Duplicate milk samples were collected from January to February transferred to the laboratory and analyzed for the presence of Streptococcus spp. by using the serial dilution method and plating on the surface of Slanetz and Bartley agar. The isolates were tested against nine antimicrobial agents with the Kirby-Bauer disk diffusion method. The results showed that Streptococcus spp. occurred in 84.4% of the samples and in counts ranged between 1.8 log CFU/mL and 4.6 log CFU/mL. Concerning susceptibility, only 1.3% of the isolates were resistant to all antimicrobials while 27.3% were fully susceptible. Resistance to cefepime was the most common （93.1%） observation followed by resistance to ampicillin （82.1%）, ciprofloxacine （58.3%） and meropenem （51.9%）. The isolates were vancomycin susceptible at a rate of 83.6%. Multidrug resistance to at least three antibiotics was observed to be 22.1% of Streptococcus spp.. Given the prevalence of Streptococcus spp. and the elevated resistance of isolates to various antibiotics it is concluded that further examinations and even interventions are required in order to pertain the appropriate animal health and milk quality.展开更多
The distribution of the scincid lizard genus Lygosoma in Thailand was studied and recorded on a map. In addition, the studies on chromosomes of Lygosoma bowringii, L. khoratense and L. quadrupes were examined. The kar...The distribution of the scincid lizard genus Lygosoma in Thailand was studied and recorded on a map. In addition, the studies on chromosomes of Lygosoma bowringii, L. khoratense and L. quadrupes were examined. The karyotype was determined for each individual on the basis of at least 10 well spread cells. L. bowringii and L. khoratense showed the same diploid number of 2N = 32 and identical chromosome morphology. The fundamental number (N.F.) equals 48. The karyotype of L. quadrupes is almost identical to that of L. bowringii, but differs from the latter in having a telocentric pair 9. Thus, the N.F. value equal to 46. No sex chromosome heteromorphisms is evident.展开更多
文摘The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the animal flocks and the current trends of antibiotic use in small ruminants. A total of 77 mountainous sheepfolds were investigated representing 10% of all transhumant sheep and goats flocks in the area. Duplicate milk samples were collected from January to February transferred to the laboratory and analyzed for the presence of Streptococcus spp. by using the serial dilution method and plating on the surface of Slanetz and Bartley agar. The isolates were tested against nine antimicrobial agents with the Kirby-Bauer disk diffusion method. The results showed that Streptococcus spp. occurred in 84.4% of the samples and in counts ranged between 1.8 log CFU/mL and 4.6 log CFU/mL. Concerning susceptibility, only 1.3% of the isolates were resistant to all antimicrobials while 27.3% were fully susceptible. Resistance to cefepime was the most common （93.1%） observation followed by resistance to ampicillin （82.1%）, ciprofloxacine （58.3%） and meropenem （51.9%）. The isolates were vancomycin susceptible at a rate of 83.6%. Multidrug resistance to at least three antibiotics was observed to be 22.1% of Streptococcus spp.. Given the prevalence of Streptococcus spp. and the elevated resistance of isolates to various antibiotics it is concluded that further examinations and even interventions are required in order to pertain the appropriate animal health and milk quality.
文摘The distribution of the scincid lizard genus Lygosoma in Thailand was studied and recorded on a map. In addition, the studies on chromosomes of Lygosoma bowringii, L. khoratense and L. quadrupes were examined. The karyotype was determined for each individual on the basis of at least 10 well spread cells. L. bowringii and L. khoratense showed the same diploid number of 2N = 32 and identical chromosome morphology. The fundamental number (N.F.) equals 48. The karyotype of L. quadrupes is almost identical to that of L. bowringii, but differs from the latter in having a telocentric pair 9. Thus, the N.F. value equal to 46. No sex chromosome heteromorphisms is evident.
