RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a speci...RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiF e-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiF e-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin.Cell lines such as Calu3, Hela, A549, CaF br, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost,easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.展开更多
Holographic optical elements(HOEs)are integral to advancements in optical sensing,augmented reality,solar energy harvesting,biomedical diagnostics,and many other fields,offering precise and versatile light manipulatio...Holographic optical elements(HOEs)are integral to advancements in optical sensing,augmented reality,solar energy harvesting,biomedical diagnostics,and many other fields,offering precise and versatile light manipulation capabilities.This study,to the best of the authors'knowledge,is the first to design and fabricate an HOE mutliwaveguide system using a thermally and environmentally stable photopolymerizable hybrid sol-gel(PHSG)for sensing applications.Using a 476.5 nm recording wavelength,60%diffraction efficiency PHSG holographic waveguides of spatial frequency of 1720 lines/mm were successfully fabricated to function as in-and out-couplers at 632.8 nm and 700 nm wavelength,respectively.The waveguides were integrated into a polydimethylsiloxane(PDMS)microfluidic system,guiding excitation light of 632.8 nm wavelength into and extracting fluorescence light signal peaking at 700 nm from a location filled with methylene blue water solution.Further,to demonstrate the potential of the proposed optical system,four holographic waveguides were recorded by peristrophic and angular multiplexing in the same location of the material and the input beam was delivered into four spatially separated channels by total internal reflection in the sol-gel layer,thus,successfully highlighting the capabilities and advantages of HOE waveguides for parallel interrogation of multiple locations in a wearable sensor.This study demonstrates the efficiency and versatility of PHSG-based HOE waveguides,underscoring their potential to enhance photonic device design and performance across various optical applications.展开更多
基金supported by National Key Basic Research Program (973 Project) (No. 2010CB933901 and 2011CB933100)National 863 Hi-tech Project of China (No. 2012AA022703), National Natural Scientific Fund (No. 81225010, 81101169 and 31100717)Shanghai Nano project (13NM1401500), Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110073120072)
文摘RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiF e-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiF e-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin.Cell lines such as Calu3, Hela, A549, CaF br, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost,easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.
基金funded by the Irish Government's Program for Research in Third-Level Institutions,Cycle 4,National Development Plan 2007-2013supported by the European Union Structural Fund
基金European Space Agency(4000129503/20/NL/PG/pt)Science Foundation Ireland(20/FFP-P/8851)。
文摘Holographic optical elements(HOEs)are integral to advancements in optical sensing,augmented reality,solar energy harvesting,biomedical diagnostics,and many other fields,offering precise and versatile light manipulation capabilities.This study,to the best of the authors'knowledge,is the first to design and fabricate an HOE mutliwaveguide system using a thermally and environmentally stable photopolymerizable hybrid sol-gel(PHSG)for sensing applications.Using a 476.5 nm recording wavelength,60%diffraction efficiency PHSG holographic waveguides of spatial frequency of 1720 lines/mm were successfully fabricated to function as in-and out-couplers at 632.8 nm and 700 nm wavelength,respectively.The waveguides were integrated into a polydimethylsiloxane(PDMS)microfluidic system,guiding excitation light of 632.8 nm wavelength into and extracting fluorescence light signal peaking at 700 nm from a location filled with methylene blue water solution.Further,to demonstrate the potential of the proposed optical system,four holographic waveguides were recorded by peristrophic and angular multiplexing in the same location of the material and the input beam was delivered into four spatially separated channels by total internal reflection in the sol-gel layer,thus,successfully highlighting the capabilities and advantages of HOE waveguides for parallel interrogation of multiple locations in a wearable sensor.This study demonstrates the efficiency and versatility of PHSG-based HOE waveguides,underscoring their potential to enhance photonic device design and performance across various optical applications.
