This paper presents a fringe-carrier method that eliminates sign ambiguity of transient moire fringes which can be used to automatically determine the relative orders. A fringe carrier is preset in the static state of...This paper presents a fringe-carrier method that eliminates sign ambiguity of transient moire fringes which can be used to automatically determine the relative orders. A fringe carrier is preset in the static state of the specimen and the dynamic in-plane displacements are recorded as the modulation to the frequency of the carrier fringes when the specimen is loaded by impact. According to a modulating criterion developed from the modulation degree, the fringes of the transient moire patterns keep monotonical in orders so that they can be automatically encoded in grey levels by a digital image system. The moire orders purely caused by dynamic loadings are evaluated by subtracting the grey-value of the unmodulated carrier image from that of the modulated carrier images encoded by their orders. With the subtracted moire orders the strain components can be obtained, and, correspondingly, the histograms of dynamic displacement moire images are shown with order variation by image-difference.展开更多
Understanding cell-material interactions is crucial for advancing biomedical applications,influencing cellular behavior and medical device performance.Material properties can be manipulated to direct cell responses,be...Understanding cell-material interactions is crucial for advancing biomedical applications,influencing cellular behavior and medical device performance.Material properties can be manipulated to direct cell responses,benefiting applications from regenerative medicine to implantable devices such as silicone breast implants.Knowledge about the interaction differences between healthy and cancer cells with implants may guide implant design to more precisely influence cell adhesion and proliferation of healthy cells while inhibiting cancer cells,tailoring outcomes to specific cellular responses.To show-case this potential,breast epithelial cells and breast cancer cells were investigated regarding their interaction with a broad range of combined physicochemical properties.This study employed a silicone-based high-throughput screening method utilizing Double Orthogonal Gradients(DOGs)to investigate the influence of topography,stiffness,and wettability on breast epithelial cells(MCF10a)and breast cancer cells(MCF7).Results show distinct cellular responses,including decreased prolif-eration rates in both MCF10a and MCF7 cells with the introduction of surface topography and the dominant influence of wettability on cell adhesion,proliferation,and cluster formation.The screening identified specific regions of interest(ROIs)where MCF10a cell proliferation outperformed MCF7 cells and that topography inhibits cluster formation(tumorigenesis),offering potential prospects for the creation of novel implant surfaces.展开更多
基金The project supported by Alexander von Humboldt Foundation or Germany and the National Natural Science Foundation of China
文摘This paper presents a fringe-carrier method that eliminates sign ambiguity of transient moire fringes which can be used to automatically determine the relative orders. A fringe carrier is preset in the static state of the specimen and the dynamic in-plane displacements are recorded as the modulation to the frequency of the carrier fringes when the specimen is loaded by impact. According to a modulating criterion developed from the modulation degree, the fringes of the transient moire patterns keep monotonical in orders so that they can be automatically encoded in grey levels by a digital image system. The moire orders purely caused by dynamic loadings are evaluated by subtracting the grey-value of the unmodulated carrier image from that of the modulated carrier images encoded by their orders. With the subtracted moire orders the strain components can be obtained, and, correspondingly, the histograms of dynamic displacement moire images are shown with order variation by image-difference.
基金the UMCG Microscopy and Imaging Center(UMIC)for use of microscopy equipment(sponsored by NWO 40-00506-98-9021)the Graduate School Medical Sciences(GSMS).
文摘Understanding cell-material interactions is crucial for advancing biomedical applications,influencing cellular behavior and medical device performance.Material properties can be manipulated to direct cell responses,benefiting applications from regenerative medicine to implantable devices such as silicone breast implants.Knowledge about the interaction differences between healthy and cancer cells with implants may guide implant design to more precisely influence cell adhesion and proliferation of healthy cells while inhibiting cancer cells,tailoring outcomes to specific cellular responses.To show-case this potential,breast epithelial cells and breast cancer cells were investigated regarding their interaction with a broad range of combined physicochemical properties.This study employed a silicone-based high-throughput screening method utilizing Double Orthogonal Gradients(DOGs)to investigate the influence of topography,stiffness,and wettability on breast epithelial cells(MCF10a)and breast cancer cells(MCF7).Results show distinct cellular responses,including decreased prolif-eration rates in both MCF10a and MCF7 cells with the introduction of surface topography and the dominant influence of wettability on cell adhesion,proliferation,and cluster formation.The screening identified specific regions of interest(ROIs)where MCF10a cell proliferation outperformed MCF7 cells and that topography inhibits cluster formation(tumorigenesis),offering potential prospects for the creation of novel implant surfaces.
