“Build Your Dreams”(成就你的梦想)这一简洁且催人奋进的口号正是比亚迪(深圳)汽车有限公司英文缩写“BYD”背后所蕴含的意思,并且可能已成为引领公司取得全球成功的重要座右铭.比亚迪汽车有限公司成立于2003年,在致力于为各年龄段...“Build Your Dreams”(成就你的梦想)这一简洁且催人奋进的口号正是比亚迪(深圳)汽车有限公司英文缩写“BYD”背后所蕴含的意思,并且可能已成为引领公司取得全球成功的重要座右铭.比亚迪汽车有限公司成立于2003年,在致力于为各年龄段消费者打造梦寐以求产品的同时,近几年呈现出惊人的发展速度.公司通过垂直整合和最新能源进展的创新电动汽车和技术,从比亚迪股份有限公司这一控股集团旗下的数个兄弟公司中脱颖而出.展开更多
Seafloor extension and associated rifting in the North Atlantic Area, which started in Early Paleogene (from −62 Ma), resulted in a few micro-continents being isolated and submerged below sea levels. Published isotopi...Seafloor extension and associated rifting in the North Atlantic Area, which started in Early Paleogene (from −62 Ma), resulted in a few micro-continents being isolated and submerged below sea levels. Published isotopic data for sunken offshore continental materials (basement) in the North Atlantic area are quite sparse, but a few do exist for the Rockall Plateau, or more precisely the Rockall Bank. Isotopic data for Early Paleogene basaltic materials, covering basement rocks of the Rockall Plateau, have hitherto only been publicised for the NW margin of the Hatton bank. The Early Paleogene basaltic archipelago of the Faroe Islands, on which some isotopic data do exist, rests on an ancient sunken continental crust of unknown geochemical and isotopic compositions. The objective of this contribution is to assess potential lead isotopic relationships between the Rockall Plateau and the Faroese sub-basaltic basement, based on the sparse available isotopic data existing for the former and using available isotopic data for slightly contaminated Faroese basaltic rocks. The results reached in this contribution point to a likely association between the Faroese sub-basaltic basement and the basement of the Rockall Bank and hence the Rockall Plateau and potentially also between Faroese basaltic rocks and contemporaneous counterparts from the Hatton Bank.展开更多
Psychiatric Day Hospitals are alternatives to classic hospitalization, staying in an intermediate position between full-time hospitalization and outpatient treatment. The main purpose of this work was to investigate t...Psychiatric Day Hospitals are alternatives to classic hospitalization, staying in an intermediate position between full-time hospitalization and outpatient treatment. The main purpose of this work was to investigate the impact of the therapeutic program developed in a Psychiatric Day Hospital (of Faro-Portugal) on the clinical stability of the patients. It is a descriptive-correlational retrospective study, implemented through the consultation of the SCLINIC computer platform regarding the years 2015, 2016 and 2017. The records of 127 users were analysed. There was a predominance of mood disorders (n = 55;43.3%), followed by schizophrenia, schizotypal and delusional disorders (n = 43, 33.9%). The majority of patients had a structured occupational plan after discharge (n = 106, 83.46%). In the year following the frequency of Day Hospital, compared to the year prior to admission, there was a reduction in the number of full-time hospitalizations, outpatient visitations/consultations, prescribed drugs and emergency episodes, with statistically significant differences. The positive impact of the frequency of Day Hospital had on patients’ clinical stability was the improvement in all studied variables. The Psychiatric Day Hospital of Faro-Portugal contributes to decreasing the number of full time hospitalizations, outpatient visitations/consultations and emergency episodes, maintaining patients’ clinical stability and reducing the public financial costs related to their therapeutic intervention.展开更多
Northern Fulmars(Fulmarus glacialis)are a pelagic seabird species distributed at northern and polar latitudes.They are often used as an indicator of plastic pollution in the North Sea region,but data are lacking from ...Northern Fulmars(Fulmarus glacialis)are a pelagic seabird species distributed at northern and polar latitudes.They are often used as an indicator of plastic pollution in the North Sea region,but data are lacking from higher latitudes,especially when it comes to chicks.Here,we investigated amounts of ingested plastic and their characteristics in fulmar chicks from the Faroe Islands.Plastic particles(≥1 mm)in chicks of two age classes were searched using a digestion method with KOH.In addition,to evaluate if additive tissue burden reflects plastic ingestion,we measured liver tissue concentrations of two pollutant classes associated with plastic materials:polybrominated diphenyl ethers(PBDEs)and several dechloranes,using gas chromatography with high-resolution mass spectrometry.The most common shape was hard fragment(81%)and the most common polymer was polyethylene(73%).Plastic contamination did not differ between either age class,and we found no correlation between neither the amount and mass of plastic particles and the concentration of additives.After comparison with previous studies on adult fulmars,we do not recommend using chicks for biomonitoring adults because chicks seem to ingest more plastics than adults.展开更多
With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether th...With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether they are physiological or pathological.展开更多
In this paper,we present a modeling of the soil-water characteristic curve for residual and sedimentary soils of Bom Brinquedo Hill’s,located in Antonina,Brazil.This mountain range region is characterized as a natura...In this paper,we present a modeling of the soil-water characteristic curve for residual and sedimentary soils of Bom Brinquedo Hill’s,located in Antonina,Brazil.This mountain range region is characterized as a natural disaster risk area,requiring continuous research related to the stability of the area.To obtain the soil-water characteristic curve,undisturbed samples of residual and sedimentary soil were collected,followed by suction testing using the filter paper method.Considering the bimodal characteristic presented by the soil,LABFIT software was employed for curve fitting using the generic formulation“Harris+C”.The results of the tests indicated that the phenomenon of hysteresis had a greater influence in situations with higher suction levels.When comparing the residual moisture values of the macropores between residual soil and sedimentary soil,the former exhibited the lower value.This suggests that the residual soil has a coarser grain size and larger pores,which facilitates the release of water retained in the soil’s macropores.展开更多
The potential of induced pluripotent stem cells(iPSCs)for modeling and treating metabolic associated fatty liver disease(MAFLD)and metabolic associated steatohepatitis(MASH)is emerging.MAFLD is a growing global health...The potential of induced pluripotent stem cells(iPSCs)for modeling and treating metabolic associated fatty liver disease(MAFLD)and metabolic associated steatohepatitis(MASH)is emerging.MAFLD is a growing global health concern,currently with limited treatment options.While primary mesenchymal stem cells hold promise,iPSCs offer a versatile alternative due to their ability to differentiate into various cell types,including iPSC-derived mesenchymal stem cells.However,challenges remain,including optimizing differentiation protocols,ensuring cell safety,and addressing potential tumorigenicity risks.In addition,iPSCs offer the possibility to generate complex cellular models,including three-dimensional organoid models,which are closer representations of the human disease than animal models.Those models would also be valuable for drug discovery and personalized medicine approaches.Overall,iPSCs and their derivatives offer new perspectives for advancing MAFLD/MASH research and developing novel therapeutic strategies.Further research is needed to overcome current limitations and translate this potential into effective clinical applications.展开更多
利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素(somatostatin,SST)基因(sst1)突变体鱼系。通过比较6 dpf(days post fertilization,dpf)的sst1^(-/-)突变体和野生型仔鱼转录组,发现sst1^(-/-)突变体相关生化过程和...利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素(somatostatin,SST)基因(sst1)突变体鱼系。通过比较6 dpf(days post fertilization,dpf)的sst1^(-/-)突变体和野生型仔鱼转录组,发现sst1^(-/-)突变体相关生化过程和信号通路发生了显著变化。相比野生型,sst1^(-/-)突变体仔鱼中有354个基因显著上调,504个基因显著下调。GO(gene ontology)富集分析表明,大部分差异基因与氨基酸和蛋白质生物合成相关。KEGG(kyoto encyclopedia of genes and genomes)通路富集显示代谢通路、氨基酸合成、氨基酰基-tRNA合成、核糖体合成和rRNA加工相关通路表达增强。结果表明,sst1^(-/-)突变体蛋白质生物合成和代谢过程更加活跃。RT-qPCR验证结果显示,随机选取的10个基因mRNA表达变化趋势与转录组测序结果一致,说明转录组测序结果真实可靠。6月龄突变体体质量、体长和野生型相比并无显著差异。本研究结果为进一步挖掘生长抑素基因家族的潜在功能奠定基础。展开更多
Induced pluripotent stem cells(iPSC)technology has propelled the field of stem cells biology,providing new cells to explore the molecular mechanisms of pluripotency,cancer biology and aging.A major advantage of human ...Induced pluripotent stem cells(iPSC)technology has propelled the field of stem cells biology,providing new cells to explore the molecular mechanisms of pluripotency,cancer biology and aging.A major advantage of human iPSC,compared to the pluripotent embryonic stem cells,is that they can be generated from virtually any embryonic or adult somatic cell type without destruction of human blastocysts.In addition,iPSC can be generated from somatic cells harvested from normal individuals or patients,and used as a cellular tool to unravel mechanisms of human development and to model diseases in a manner not possible before.Besides these fundamental aspects of human biology and physiology that are revealed using iPSC or iPSC-derived cells,these cells hold an immense potential for cell-based therapies,and for the discovery of new or personalized pharmacological treatments for many disorders.Here,we review some of the current challenges and concerns about iPSC technology.We introduce the potential held by iPSC for research and development of novel health-related applications.We briefly present the efforts made by the scientific and clinical communities to create the necessary guidelines and regulations to achieve the highest quality standards in the procedures for iPSC generation,characterization and long-term preservation.Finally,we present some of the audacious and pioneer clinical trials in progress with iPSC-derived cells.展开更多
The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decame...The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V10) clearly differs from monomeric vanadate (V1), and affects differently calcium pumps, and structure and function of myosin and actin. Only decavanadate inhibits calcium accumulation by calcium pump ATPase, and strongly inhibits actomyosin ATPase activity (IC50 = 1.4 μmol/L, V10), whereas no such ef- fects are detected with V1 up to 150 μmol/L; prevents actin polymerization (IC50 of 68 μmol/L, whereas no effects detected with up to 2 mmol/L V1); and interacts with actin in a way that induces cysteine oxidation and vanadate reduction to vanadyl. Moreover, in vivo decavanadate toxicity studies have revealed that acute exposure to polyoxovanadate induces different changes in antioxidant enzymes and oxidative stress parameters, in comparison with vanadate. In vitro studies have clearly demonstrated that mitochondrial oxygen consumption is strongly affected by decavanadate (IC50, 0.1 μmol/L); perhaps the most relevant biological effect. Finally, decavanadate (100 μmol/L) increases rat adipocyte glucose accumulation more potently than several vanadium complexes. Preliminary studies sug- gest that decavanadate does not have similar effects in human adipocytes. Although decavanadate can be a useful biochemical tool, further studies must be carried out before it can be conf irmed that decavanadate and its complexes can be used as anticancer or antidiabetic agents.展开更多
文摘“Build Your Dreams”(成就你的梦想)这一简洁且催人奋进的口号正是比亚迪(深圳)汽车有限公司英文缩写“BYD”背后所蕴含的意思,并且可能已成为引领公司取得全球成功的重要座右铭.比亚迪汽车有限公司成立于2003年,在致力于为各年龄段消费者打造梦寐以求产品的同时,近几年呈现出惊人的发展速度.公司通过垂直整合和最新能源进展的创新电动汽车和技术,从比亚迪股份有限公司这一控股集团旗下的数个兄弟公司中脱颖而出.
文摘Seafloor extension and associated rifting in the North Atlantic Area, which started in Early Paleogene (from −62 Ma), resulted in a few micro-continents being isolated and submerged below sea levels. Published isotopic data for sunken offshore continental materials (basement) in the North Atlantic area are quite sparse, but a few do exist for the Rockall Plateau, or more precisely the Rockall Bank. Isotopic data for Early Paleogene basaltic materials, covering basement rocks of the Rockall Plateau, have hitherto only been publicised for the NW margin of the Hatton bank. The Early Paleogene basaltic archipelago of the Faroe Islands, on which some isotopic data do exist, rests on an ancient sunken continental crust of unknown geochemical and isotopic compositions. The objective of this contribution is to assess potential lead isotopic relationships between the Rockall Plateau and the Faroese sub-basaltic basement, based on the sparse available isotopic data existing for the former and using available isotopic data for slightly contaminated Faroese basaltic rocks. The results reached in this contribution point to a likely association between the Faroese sub-basaltic basement and the basement of the Rockall Bank and hence the Rockall Plateau and potentially also between Faroese basaltic rocks and contemporaneous counterparts from the Hatton Bank.
文摘Psychiatric Day Hospitals are alternatives to classic hospitalization, staying in an intermediate position between full-time hospitalization and outpatient treatment. The main purpose of this work was to investigate the impact of the therapeutic program developed in a Psychiatric Day Hospital (of Faro-Portugal) on the clinical stability of the patients. It is a descriptive-correlational retrospective study, implemented through the consultation of the SCLINIC computer platform regarding the years 2015, 2016 and 2017. The records of 127 users were analysed. There was a predominance of mood disorders (n = 55;43.3%), followed by schizophrenia, schizotypal and delusional disorders (n = 43, 33.9%). The majority of patients had a structured occupational plan after discharge (n = 106, 83.46%). In the year following the frequency of Day Hospital, compared to the year prior to admission, there was a reduction in the number of full-time hospitalizations, outpatient visitations/consultations, prescribed drugs and emergency episodes, with statistically significant differences. The positive impact of the frequency of Day Hospital had on patients’ clinical stability was the improvement in all studied variables. The Psychiatric Day Hospital of Faro-Portugal contributes to decreasing the number of full time hospitalizations, outpatient visitations/consultations and emergency episodes, maintaining patients’ clinical stability and reducing the public financial costs related to their therapeutic intervention.
基金funded by the Fram Centre Research Programme“Plastic in the Arctic”through the PlastFul project(PA072018)supported by the Norwegian Polar Institute and the PlastPoll project funded by the Research Council of Norway(#275172)This research was partly financed by The Research Council of Norway,project no.160022/F40 NINA basic funding。
文摘Northern Fulmars(Fulmarus glacialis)are a pelagic seabird species distributed at northern and polar latitudes.They are often used as an indicator of plastic pollution in the North Sea region,but data are lacking from higher latitudes,especially when it comes to chicks.Here,we investigated amounts of ingested plastic and their characteristics in fulmar chicks from the Faroe Islands.Plastic particles(≥1 mm)in chicks of two age classes were searched using a digestion method with KOH.In addition,to evaluate if additive tissue burden reflects plastic ingestion,we measured liver tissue concentrations of two pollutant classes associated with plastic materials:polybrominated diphenyl ethers(PBDEs)and several dechloranes,using gas chromatography with high-resolution mass spectrometry.The most common shape was hard fragment(81%)and the most common polymer was polyethylene(73%).Plastic contamination did not differ between either age class,and we found no correlation between neither the amount and mass of plastic particles and the concentration of additives.After comparison with previous studies on adult fulmars,we do not recommend using chicks for biomonitoring adults because chicks seem to ingest more plastics than adults.
基金Clévio Nóbrega’s laboratory is funded by the Cure CSB projectthe Viljem Julijan Association for Children with Rare Diseases(Slovenia)+1 种基金the Algarve Biomedical Center Research Institute(ABC-Ri)funded by CRESC Algarve 2020(Operation Code:ALG-01-0145-FEDER-072586)(to CN)。
文摘With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether they are physiological or pathological.
文摘In this paper,we present a modeling of the soil-water characteristic curve for residual and sedimentary soils of Bom Brinquedo Hill’s,located in Antonina,Brazil.This mountain range region is characterized as a natural disaster risk area,requiring continuous research related to the stability of the area.To obtain the soil-water characteristic curve,undisturbed samples of residual and sedimentary soil were collected,followed by suction testing using the filter paper method.Considering the bimodal characteristic presented by the soil,LABFIT software was employed for curve fitting using the generic formulation“Harris+C”.The results of the tests indicated that the phenomenon of hysteresis had a greater influence in situations with higher suction levels.When comparing the residual moisture values of the macropores between residual soil and sedimentary soil,the former exhibited the lower value.This suggests that the residual soil has a coarser grain size and larger pores,which facilitates the release of water retained in the soil’s macropores.
基金American Heart Association Award,No.24IVPHA1288417and FCT Fellowships,No.2022.13253.BDANA.
文摘The potential of induced pluripotent stem cells(iPSCs)for modeling and treating metabolic associated fatty liver disease(MAFLD)and metabolic associated steatohepatitis(MASH)is emerging.MAFLD is a growing global health concern,currently with limited treatment options.While primary mesenchymal stem cells hold promise,iPSCs offer a versatile alternative due to their ability to differentiate into various cell types,including iPSC-derived mesenchymal stem cells.However,challenges remain,including optimizing differentiation protocols,ensuring cell safety,and addressing potential tumorigenicity risks.In addition,iPSCs offer the possibility to generate complex cellular models,including three-dimensional organoid models,which are closer representations of the human disease than animal models.Those models would also be valuable for drug discovery and personalized medicine approaches.Overall,iPSCs and their derivatives offer new perspectives for advancing MAFLD/MASH research and developing novel therapeutic strategies.Further research is needed to overcome current limitations and translate this potential into effective clinical applications.
文摘利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素(somatostatin,SST)基因(sst1)突变体鱼系。通过比较6 dpf(days post fertilization,dpf)的sst1^(-/-)突变体和野生型仔鱼转录组,发现sst1^(-/-)突变体相关生化过程和信号通路发生了显著变化。相比野生型,sst1^(-/-)突变体仔鱼中有354个基因显著上调,504个基因显著下调。GO(gene ontology)富集分析表明,大部分差异基因与氨基酸和蛋白质生物合成相关。KEGG(kyoto encyclopedia of genes and genomes)通路富集显示代谢通路、氨基酸合成、氨基酰基-tRNA合成、核糖体合成和rRNA加工相关通路表达增强。结果表明,sst1^(-/-)突变体蛋白质生物合成和代谢过程更加活跃。RT-qPCR验证结果显示,随机选取的10个基因mRNA表达变化趋势与转录组测序结果一致,说明转录组测序结果真实可靠。6月龄突变体体质量、体长和野生型相比并无显著差异。本研究结果为进一步挖掘生长抑素基因家族的潜在功能奠定基础。
文摘Induced pluripotent stem cells(iPSC)technology has propelled the field of stem cells biology,providing new cells to explore the molecular mechanisms of pluripotency,cancer biology and aging.A major advantage of human iPSC,compared to the pluripotent embryonic stem cells,is that they can be generated from virtually any embryonic or adult somatic cell type without destruction of human blastocysts.In addition,iPSC can be generated from somatic cells harvested from normal individuals or patients,and used as a cellular tool to unravel mechanisms of human development and to model diseases in a manner not possible before.Besides these fundamental aspects of human biology and physiology that are revealed using iPSC or iPSC-derived cells,these cells hold an immense potential for cell-based therapies,and for the discovery of new or personalized pharmacological treatments for many disorders.Here,we review some of the current challenges and concerns about iPSC technology.We introduce the potential held by iPSC for research and development of novel health-related applications.We briefly present the efforts made by the scientific and clinical communities to create the necessary guidelines and regulations to achieve the highest quality standards in the procedures for iPSC generation,characterization and long-term preservation.Finally,we present some of the audacious and pioneer clinical trials in progress with iPSC-derived cells.
文摘The number of papers about decavanadate has doubled in the past decade. In the present review, new insights into decavanadate biochemistry, cell biology, and antidiabetic and antitumor activities are described. Decameric vanadate species (V10) clearly differs from monomeric vanadate (V1), and affects differently calcium pumps, and structure and function of myosin and actin. Only decavanadate inhibits calcium accumulation by calcium pump ATPase, and strongly inhibits actomyosin ATPase activity (IC50 = 1.4 μmol/L, V10), whereas no such ef- fects are detected with V1 up to 150 μmol/L; prevents actin polymerization (IC50 of 68 μmol/L, whereas no effects detected with up to 2 mmol/L V1); and interacts with actin in a way that induces cysteine oxidation and vanadate reduction to vanadyl. Moreover, in vivo decavanadate toxicity studies have revealed that acute exposure to polyoxovanadate induces different changes in antioxidant enzymes and oxidative stress parameters, in comparison with vanadate. In vitro studies have clearly demonstrated that mitochondrial oxygen consumption is strongly affected by decavanadate (IC50, 0.1 μmol/L); perhaps the most relevant biological effect. Finally, decavanadate (100 μmol/L) increases rat adipocyte glucose accumulation more potently than several vanadium complexes. Preliminary studies sug- gest that decavanadate does not have similar effects in human adipocytes. Although decavanadate can be a useful biochemical tool, further studies must be carried out before it can be conf irmed that decavanadate and its complexes can be used as anticancer or antidiabetic agents.
