Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates ...Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.展开更多
This study was carried out in the Health districts of Kayanza and Gahombo. Its aim was to assess the nutritional status and associated factors of infants aged between 6 and 23 months. This descriptive and cross-sectio...This study was carried out in the Health districts of Kayanza and Gahombo. Its aim was to assess the nutritional status and associated factors of infants aged between 6 and 23 months. This descriptive and cross-sectional study was carried out in the health centers of the action zone during medical consultations in community medicine from 13 to 22 February 2023. For this purpose, 53.0% of a sample of 398 mothers of infants aged 6 - 23 months were male, and the most represented age group was 12 - 23 months with 53.8%. Mothers were asked about the diet of their children. All the children involved in this study were vaccinated, whereas 99.2% were given vitamin A supplements. The most frequent illnesses in this age group were acute respiratory infection (ARI) (73.4%), fever (66.6%), diarrhea (53.8%), and vomiting (40.5%). Analysis of nutritional status showed that infants had severe (8.3%) and moderate (70.4%) acute malnutrition. Factors associated with infant nutritional status comprised the occupation of mother (P = 0.009), level of education of mother (P = 0.0625), and prolonged diarrhea (P = 0.004). To remedy this problem, concrete nutritional and educational interventions are needed to promote optimal infant nutrition during the first two years of life.展开更多
The implementation of WHO guidelines has significantly reduced hospital mortality due to severe malnutrition. Nevertheless, severe acute malnutrition in children under five remains a major public health problem in all...The implementation of WHO guidelines has significantly reduced hospital mortality due to severe malnutrition. Nevertheless, severe acute malnutrition in children under five remains a major public health problem in all low-income countries and is little studied. The study aimed to assess the nutritional status and quality of management of severe acute malnutrition in children aged 06 to 59 months hospitalized in the pediatric ward of Kayanza Hospital. This is a prospective study with descriptive and analytical aims over 6 months from February 10 to July 9, 2023. All children aged 6 to 59 months admitted for severe acute malnutrition are included in this study. The results show that the most affected age group is 12 to 23 months (42.55%). Marasmus is the most common clinical form, at 70.2%. Housewives and mothers from rural areas are the most affected, with rates ranging from 69.14% to 91.49%. The study shows that the reasons for consultation are respectively: edema (29.78%), diarrhea (26.59%), vomiting (19.14%), and fever (14.89%). Dehydration and hypothermia were the main complications observed in 45.74% and 22.34% respectively. Medical treatment was provided by antibiotics (44.68%), artesunate (31.91%), and resomal (21.27%). Nutritional treatment consisted of F75 100% milk and F100 100% milk. Pathologies observed were: anemia 38.29%, malaria 37.23%, urinary tract infection 12.7%, measles 11.7%. The national protocol for integrated management of acute malnutrition (PCIMA) was followed. This study shows that 72.3% of children were successfully treated, with 9.5% dropping out and dying, and 8.5% not responding. We found that malnutrition remains a public health problem, affecting mostly children aged 12 to 24 months. The main complications or pathologies associated with malnutrition are diarrhea, malaria, and fever, and the majority of children suffer from marasmus.展开更多
Commercial plantations of Eucalyptus species have been established in South China,especially during the past 20 years,to meet the needs of a rapidly growing national economy.As part of a survey of fungal diseases affe...Commercial plantations of Eucalyptus species have been established in South China,especially during the past 20 years,to meet the needs of a rapidly growing national economy.As part of a survey of fungal diseases affecting Eucalyptus species in South China,Ceratocystis species were collected from Eucalyptus plantations in the GuangDong Province.The aims of this study were to identify these Ceratocystis isolates and to test their pathogenicity to Eucalyptus.The most aggressive isolates were also used to screen different species and clones of Eucalyptus for susceptibility to infection under field conditions.The fungi were identified based on morphology and through comparisons of DNA sequence data of the ITS,partialβ-tubulin and TEF-1αgene regions.Morphological and DNA sequence comparisons showed that isolates collected from Chinese Eucalyptus plantations represent two species,C.acaciivora in the C.fimbriata s.l.species complex and a previously undescribed species belonging to the C.moniliformis s.l.species complex,for which the name C.chinaeucensis sp.nov.is provided.In pathogenicity trials,both C.acaciivora and C.chinaeucensis gave rise to lesions on wounded Eucalyptus trees,and the former fungus was most pathogenic.Differences were also observed in the responses of different Eucalyptus clones to inoculation and this could be useful in reducing disease,if C.acaciivora should emerge as a pathogen in the future.展开更多
The utilization of urea in camels has beneficial and negative effects. The aims of this study were to investigate the effects of different levels of urea supplementation on nutrients intake, digestibility,growth perfo...The utilization of urea in camels has beneficial and negative effects. The aims of this study were to investigate the effects of different levels of urea supplementation on nutrients intake, digestibility,growth performance, feed efficiency and economics in growing camels fed roughage based complete pellet diets. In the present study, eighteen growing camels with an average live body weight of306.17 ± 2.05 kg were randomly assigned in three treatments: T1 = roughage complete pellet diet without urea,T2 = T1 plus 1% urea,and T3 = T1 plus 2% urea. The results showed that the urea supplementation significantly affected average daily feed and nutrient intake of dry matter(DM), organic matter(OM), crude protein(CP), neutral detergent fiber(NDF), and acid detergent fiber(ADF)(P < 0.05).On the contrary, the average daily intake of nitrogen free extract(NFE) and water were not influenced by increasing urea supplementation(P > 0.05). Similarly, digestion coefficient of DM, CP, ether extract(EE),crude fiber(CF) and ADF was influenced by increasing urea level(P < 0.05), while the digestion coefficient of OM, NFE and NDF was not affected by increasing urea level(P > 0.05). The intake of digestive nutrients was similar among all treatment groups. Total body live weight gain and average daily gain were significantly higher in urea supplemented groups(P < 0.05) than in the control group. The supplementation of urea at 1% in low quality roughage complete pellet diets significantly improved(P < 0.05) the feed efficiency. In conclusion,these results indicated that the incorporation of urea at 1% in roughage based complete pellet diets could positively improve nutrients intake, digestibility, growth performance and feed conversion efficiency of growing camels.展开更多
The use of herbal medicines is gaining importance,even in Western medicine.With any herbal extract/medicine/however,there are many unknown components,which are often only present in low amounts.Identifying only one or...The use of herbal medicines is gaining importance,even in Western medicine.With any herbal extract/medicine/however,there are many unknown components,which are often only present in low amounts.Identifying only one or some compounds hardly describes the complex extracts,and will thus not be reliable enough for their quality control.A possible solution is the use of’fingerprint technology’,which can,for example,be performed by means展开更多
Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable environment.Various plants have been genetically improved mostly through breeding,along with limited modification via genet...Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable environment.Various plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches.This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems.Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes.From this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology research.We highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of research.Finally,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance.展开更多
Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each funga...Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each fungal species can have one nomenclaturally correct name in a particular classification.All other previously used names for this species will be considered as synonyms.The older generic epithet takes priority over the younger name.Any widely used younger names proposed for use,must comply with Art.57.2 and their usage should be approved by the Nomenclature Committee for Fungi(NCF).In this paper,we list all genera currently accepted by us in Dothideomycetes(belonging to 23 orders and 110 families),including pleomorphic and nonpleomorphic genera.In the case of pleomorphic genera,we follow the rulings of the current ICN and propose single generic names for future usage.The taxonomic placements of 1261 genera are listed as an outline.Protected names and suppressed names for 34 pleomorphic genera are listed separately.Notes and justifications are provided for possible proposed names after the list of genera.Notes are also provided on recent advances in our understanding of asexual and sexual morph linkages in Dothideomycetes.A phylogenetic tree based on four gene analyses supported 23 orders and 75 families,while 35 families still lack molecular data.展开更多
The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies.Over the past decades,rapid development and affordability of molecular tools have tremendously improved i...The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies.Over the past decades,rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats.Yet,in spite of the progress of molecular methods,knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging.In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels.Combining the information from previous efforts such as FUNGuild and FunFun together with involvement of expert knowledge,we reannotated 10,210 and 151 fungal and Stramenopila genera,respectively.This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera,designed for rapid functional assignments of environmental stud-ies.In order to assign the trait states to fungal species hypotheses,the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences.On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1%dissimilarity threshold.展开更多
Correction to:Fungal Diversity(2020)105:116 https://doi.org/10.1007/s13225-020-00466-2 There were errors in the name of author LászlóG.Nagy and in affiliation no.31 in the original publication.The original a...Correction to:Fungal Diversity(2020)105:116 https://doi.org/10.1007/s13225-020-00466-2 There were errors in the name of author LászlóG.Nagy and in affiliation no.31 in the original publication.The original article has been corrected.展开更多
文摘Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.
文摘This study was carried out in the Health districts of Kayanza and Gahombo. Its aim was to assess the nutritional status and associated factors of infants aged between 6 and 23 months. This descriptive and cross-sectional study was carried out in the health centers of the action zone during medical consultations in community medicine from 13 to 22 February 2023. For this purpose, 53.0% of a sample of 398 mothers of infants aged 6 - 23 months were male, and the most represented age group was 12 - 23 months with 53.8%. Mothers were asked about the diet of their children. All the children involved in this study were vaccinated, whereas 99.2% were given vitamin A supplements. The most frequent illnesses in this age group were acute respiratory infection (ARI) (73.4%), fever (66.6%), diarrhea (53.8%), and vomiting (40.5%). Analysis of nutritional status showed that infants had severe (8.3%) and moderate (70.4%) acute malnutrition. Factors associated with infant nutritional status comprised the occupation of mother (P = 0.009), level of education of mother (P = 0.0625), and prolonged diarrhea (P = 0.004). To remedy this problem, concrete nutritional and educational interventions are needed to promote optimal infant nutrition during the first two years of life.
文摘The implementation of WHO guidelines has significantly reduced hospital mortality due to severe malnutrition. Nevertheless, severe acute malnutrition in children under five remains a major public health problem in all low-income countries and is little studied. The study aimed to assess the nutritional status and quality of management of severe acute malnutrition in children aged 06 to 59 months hospitalized in the pediatric ward of Kayanza Hospital. This is a prospective study with descriptive and analytical aims over 6 months from February 10 to July 9, 2023. All children aged 6 to 59 months admitted for severe acute malnutrition are included in this study. The results show that the most affected age group is 12 to 23 months (42.55%). Marasmus is the most common clinical form, at 70.2%. Housewives and mothers from rural areas are the most affected, with rates ranging from 69.14% to 91.49%. The study shows that the reasons for consultation are respectively: edema (29.78%), diarrhea (26.59%), vomiting (19.14%), and fever (14.89%). Dehydration and hypothermia were the main complications observed in 45.74% and 22.34% respectively. Medical treatment was provided by antibiotics (44.68%), artesunate (31.91%), and resomal (21.27%). Nutritional treatment consisted of F75 100% milk and F100 100% milk. Pathologies observed were: anemia 38.29%, malaria 37.23%, urinary tract infection 12.7%, measles 11.7%. The national protocol for integrated management of acute malnutrition (PCIMA) was followed. This study shows that 72.3% of children were successfully treated, with 9.5% dropping out and dying, and 8.5% not responding. We found that malnutrition remains a public health problem, affecting mostly children aged 12 to 24 months. The main complications or pathologies associated with malnutrition are diarrhea, malaria, and fever, and the majority of children suffer from marasmus.
基金study was initiated through the bilateral agreement between South Africa and China,and funded through the projects of 2012DFG31830,2010KJCX015-03 and 10145624536-400000.
文摘Commercial plantations of Eucalyptus species have been established in South China,especially during the past 20 years,to meet the needs of a rapidly growing national economy.As part of a survey of fungal diseases affecting Eucalyptus species in South China,Ceratocystis species were collected from Eucalyptus plantations in the GuangDong Province.The aims of this study were to identify these Ceratocystis isolates and to test their pathogenicity to Eucalyptus.The most aggressive isolates were also used to screen different species and clones of Eucalyptus for susceptibility to infection under field conditions.The fungi were identified based on morphology and through comparisons of DNA sequence data of the ITS,partialβ-tubulin and TEF-1αgene regions.Morphological and DNA sequence comparisons showed that isolates collected from Chinese Eucalyptus plantations represent two species,C.acaciivora in the C.fimbriata s.l.species complex and a previously undescribed species belonging to the C.moniliformis s.l.species complex,for which the name C.chinaeucensis sp.nov.is provided.In pathogenicity trials,both C.acaciivora and C.chinaeucensis gave rise to lesions on wounded Eucalyptus trees,and the former fungus was most pathogenic.Differences were also observed in the responses of different Eucalyptus clones to inoculation and this could be useful in reducing disease,if C.acaciivora should emerge as a pathogen in the future.
基金funded by NSFC(3150196431501965+1 种基金31402088)research program of State Key Laboratory of Food Science and Technology,Nanchang University(Project No.SKLF-ZZB-201509)
文摘The utilization of urea in camels has beneficial and negative effects. The aims of this study were to investigate the effects of different levels of urea supplementation on nutrients intake, digestibility,growth performance, feed efficiency and economics in growing camels fed roughage based complete pellet diets. In the present study, eighteen growing camels with an average live body weight of306.17 ± 2.05 kg were randomly assigned in three treatments: T1 = roughage complete pellet diet without urea,T2 = T1 plus 1% urea,and T3 = T1 plus 2% urea. The results showed that the urea supplementation significantly affected average daily feed and nutrient intake of dry matter(DM), organic matter(OM), crude protein(CP), neutral detergent fiber(NDF), and acid detergent fiber(ADF)(P < 0.05).On the contrary, the average daily intake of nitrogen free extract(NFE) and water were not influenced by increasing urea supplementation(P > 0.05). Similarly, digestion coefficient of DM, CP, ether extract(EE),crude fiber(CF) and ADF was influenced by increasing urea level(P < 0.05), while the digestion coefficient of OM, NFE and NDF was not affected by increasing urea level(P > 0.05). The intake of digestive nutrients was similar among all treatment groups. Total body live weight gain and average daily gain were significantly higher in urea supplemented groups(P < 0.05) than in the control group. The supplementation of urea at 1% in low quality roughage complete pellet diets significantly improved(P < 0.05) the feed efficiency. In conclusion,these results indicated that the incorporation of urea at 1% in roughage based complete pellet diets could positively improve nutrients intake, digestibility, growth performance and feed conversion efficiency of growing camels.
文摘The use of herbal medicines is gaining importance,even in Western medicine.With any herbal extract/medicine/however,there are many unknown components,which are often only present in low amounts.Identifying only one or some compounds hardly describes the complex extracts,and will thus not be reliable enough for their quality control.A possible solution is the use of’fingerprint technology’,which can,for example,be performed by means
基金The writing of this manuscript was supported by the Center for Bioenergy Innovation,a U.S.Department of Energy(DOE)Bioenergy Research Center supported by the Biological and Environmental Research(BER)program,the Laboratory Directed Research and Development program of Oak Ridge National Laboratory,and the U.S.DOE BER Genomic Science Program,as part of the Secure Ecosystem Engineering and Design Scientific Focus Area and the Plant-Microbe Interfaces Scientific Focus AreaYY is supported by NSF Plant Genome Research Project Grant(1740874)and the USDA National Institute of Food and Agriculture and Hatch Appropriations under Project PEN04659 and Accession#1016432.HY is supported by Nonprofit Research Projects(CAFYBB2018ZY001-1)of Chinese Academy of Forestry+3 种基金CTT acknowledges the financial support from the NSF CAREER award(NSF#1553250)and the DOE BER Genomic Science Program(DE-SC0019412)PMS acknowledges support from the Joint BioEnergy Institute which is supported by the U.S.DOE Office of Science,BER program under Contract No.DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of EnergyDL acknowledges financial support through the National Science Foundation(NSF)under Award Number 1833402.AJM acknowledges financial support from the UK Biotechnology and Biological Sciences Research Council(grants BB/M006468/1 and BB/S015531/1)the Leverhulme Trust(grant RPG-2017-402).
文摘Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable environment.Various plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches.This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems.Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes.From this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology research.We highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of research.Finally,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance.
基金the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botanya research grant from the Biodiversity Research and Training Program(BRT R253012)+2 种基金The Thailand Research Fund(BRG 5280002)The International Scientific Cooperated Project of Guizhou Province(No[2013]7004)funding from the Spanish Ministerio de Ciencia e Innovación project CGL2011-25003.
文摘Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each fungal species can have one nomenclaturally correct name in a particular classification.All other previously used names for this species will be considered as synonyms.The older generic epithet takes priority over the younger name.Any widely used younger names proposed for use,must comply with Art.57.2 and their usage should be approved by the Nomenclature Committee for Fungi(NCF).In this paper,we list all genera currently accepted by us in Dothideomycetes(belonging to 23 orders and 110 families),including pleomorphic and nonpleomorphic genera.In the case of pleomorphic genera,we follow the rulings of the current ICN and propose single generic names for future usage.The taxonomic placements of 1261 genera are listed as an outline.Protected names and suppressed names for 34 pleomorphic genera are listed separately.Notes and justifications are provided for possible proposed names after the list of genera.Notes are also provided on recent advances in our understanding of asexual and sexual morph linkages in Dothideomycetes.A phylogenetic tree based on four gene analyses supported 23 orders and 75 families,while 35 families still lack molecular data.
基金Estonian Science Foundation grants PSG136,PRG632,PUT1170the University of Tartu(PLTOM20903)the European Regional Development Fund(Centre of Excellence EcolChange).
文摘The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies.Over the past decades,rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats.Yet,in spite of the progress of molecular methods,knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging.In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels.Combining the information from previous efforts such as FUNGuild and FunFun together with involvement of expert knowledge,we reannotated 10,210 and 151 fungal and Stramenopila genera,respectively.This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera,designed for rapid functional assignments of environmental stud-ies.In order to assign the trait states to fungal species hypotheses,the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences.On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1%dissimilarity threshold.
文摘Correction to:Fungal Diversity(2020)105:116 https://doi.org/10.1007/s13225-020-00466-2 There were errors in the name of author LászlóG.Nagy and in affiliation no.31 in the original publication.The original article has been corrected.
