This study explores the potential of silver carp by-product hydrolysate(SCBH)as a sustainable and cost-effective alternative to mucin for cultivating Akkermansia muciniphila(Akk),a beneficial probiotic known to help p...This study explores the potential of silver carp by-product hydrolysate(SCBH)as a sustainable and cost-effective alternative to mucin for cultivating Akkermansia muciniphila(Akk),a beneficial probiotic known to help prevent metabolic diseases like obesity,type II diabetes,and inflammatory bowel diseases.Traditionally,Akk relies on mucin in BHI medium,which is expensive and poses potential risks,including the transmission of swine influ-enza and restrictions due to religious considerations.We evaluated SCBH’s effects on Akk’s growth,morphology,and metabolic activity,focusing on short-chain fatty acids(SCFAs)production and the underlying mechanisms through which SCBH influences its growth and metabolism.Metabolomic analysis revealed that SCBH signifi-cantly(p<0.05)enhanced propionic,butyric,and isovaleric acid production by 19.05%,332.33%,and 35.88%,respectively.Additionally,SCBH promoted the synthesis of unsaturated fatty acid,supported amino acid metabolism,and increased taurine production while inhibiting ferroptosis,which may collectively enhancing Akk’s probiotic potential.SCBH also preserved Akk’s key morphological traits.These findings demonstrate SCBH’s efficacy as a mucin substitute,offering a novel and sustainable strategy for valorizing silver carp by-products and advancing Akk cultivation.This research provides mechanistic insights into SCBH’s role in opti-mizing Akk’s metabolic and functional properties.展开更多
Angiotensin I-converting enzyme(ACE)plays a significant role in the regulation of blood pressure via generating angiotensin II(Ang II).Using natural inhibitors to block the activity of ACE is an alternative method to ...Angiotensin I-converting enzyme(ACE)plays a significant role in the regulation of blood pressure via generating angiotensin II(Ang II).Using natural inhibitors to block the activity of ACE is an alternative method to minimize the side effects of commercial drugs,and is a major goal of hypertension management.This study detected the presence of ACE inhibitory peptides in whey protein hydrolysate.The whey protein hydrolysate was separated sequentially by ultrafiltration and RP-HPLC.LC-MS/MS revealed the amino acid sequences of the fractions and four potential ACE inhibitory peptides were selected(PQVSTPTL,MPGP,PMHIR,PPLT)for synthesis.IC50 values of these four peptides were 86±8,179±4,90±6,and 168±4μM,respectively.Then,molecular docking analysis was used to assess how these peptides interact with ACE.PQVSTPTL exhibited the lowest binding energy(6.64 kcal/mol)with the ACE molecule.These four reported peptides were with no allergenicity nor toxicity.These results indicated that whey protein hydrolysate could be a suitable resource for obtaining novel inhibitory peptides against ACE and provides information for obtaining novel ACE inhibitors for hypertension management.展开更多
基金supported by the National Key R&D Program of China(2022YFD2100905)the Fund of Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,China(NYJG 202201)the Earmarked Fund for China Agriculture Research System(CARS-45).
文摘This study explores the potential of silver carp by-product hydrolysate(SCBH)as a sustainable and cost-effective alternative to mucin for cultivating Akkermansia muciniphila(Akk),a beneficial probiotic known to help prevent metabolic diseases like obesity,type II diabetes,and inflammatory bowel diseases.Traditionally,Akk relies on mucin in BHI medium,which is expensive and poses potential risks,including the transmission of swine influ-enza and restrictions due to religious considerations.We evaluated SCBH’s effects on Akk’s growth,morphology,and metabolic activity,focusing on short-chain fatty acids(SCFAs)production and the underlying mechanisms through which SCBH influences its growth and metabolism.Metabolomic analysis revealed that SCBH signifi-cantly(p<0.05)enhanced propionic,butyric,and isovaleric acid production by 19.05%,332.33%,and 35.88%,respectively.Additionally,SCBH promoted the synthesis of unsaturated fatty acid,supported amino acid metabolism,and increased taurine production while inhibiting ferroptosis,which may collectively enhancing Akk’s probiotic potential.SCBH also preserved Akk’s key morphological traits.These findings demonstrate SCBH’s efficacy as a mucin substitute,offering a novel and sustainable strategy for valorizing silver carp by-products and advancing Akk cultivation.This research provides mechanistic insights into SCBH’s role in opti-mizing Akk’s metabolic and functional properties.
文摘Angiotensin I-converting enzyme(ACE)plays a significant role in the regulation of blood pressure via generating angiotensin II(Ang II).Using natural inhibitors to block the activity of ACE is an alternative method to minimize the side effects of commercial drugs,and is a major goal of hypertension management.This study detected the presence of ACE inhibitory peptides in whey protein hydrolysate.The whey protein hydrolysate was separated sequentially by ultrafiltration and RP-HPLC.LC-MS/MS revealed the amino acid sequences of the fractions and four potential ACE inhibitory peptides were selected(PQVSTPTL,MPGP,PMHIR,PPLT)for synthesis.IC50 values of these four peptides were 86±8,179±4,90±6,and 168±4μM,respectively.Then,molecular docking analysis was used to assess how these peptides interact with ACE.PQVSTPTL exhibited the lowest binding energy(6.64 kcal/mol)with the ACE molecule.These four reported peptides were with no allergenicity nor toxicity.These results indicated that whey protein hydrolysate could be a suitable resource for obtaining novel inhibitory peptides against ACE and provides information for obtaining novel ACE inhibitors for hypertension management.
