Objective To observe acupuncture effects, we analyze the function of Histamine on acupoints, and provide evidence for the research on generation mechanism of the acupuncture effects.
This study established the following groups of rats： a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a s...This study established the following groups of rats： a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a semen coicis group. In all but the normal and sham surgery groups the left half of Tlo was transected. Four hours after model induction, 5-Hz and 100-Hz electroacupuncture were used to stimulate the acupoints I-luantiao （GB 30）, Zusan/i （ST 36）, Zhiyan9 （DU 9） and Xuanshu （DU 5）, or crude extract from semen coicis was intraperitoneally injected, for 8 consecutive weeks. The results indicated that electroacupuncture stimulation and intraperitoneal injection of semen coicis improved the morphology of spinal cord tissue, promoted the recovery of motion-evoked potentials, suppressed glial fibrillary acidic protein expression, and ameliorated motor function in rats with hemisection spinal cord injury. The effects of high-frequency （100 Hz） electroacupuncture ancl semen coicis were significant.展开更多
Acupuncture,with its unique set of theories and its evident results in clinical practice is an important part of traditional Chinese medicine(TCM),and gaining recognition worldwide for the past few years.Although acup...Acupuncture,with its unique set of theories and its evident results in clinical practice is an important part of traditional Chinese medicine(TCM),and gaining recognition worldwide for the past few years.Although acupuncture treatment is generally accepted by the public,a few western researchers believe that sufficient evidence of its efficiency is still currently lacking,therefore creating a challenge of its acceptance on a wider scale.This article suggests the concepts of acupuncture behind clinical researches by reviewing the clinical researches done,analyzing the existing problems,and integrating real life acupuncture into the research process.By increasing the credibility of acupuncture,its reach can be extended globally and to more countries and regions.展开更多
A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of th...A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.展开更多
文摘Objective To observe acupuncture effects, we analyze the function of Histamine on acupoints, and provide evidence for the research on generation mechanism of the acupuncture effects.
基金the High-funded Projects of Japan Science and Technology Ministry,No. 343the National Natural Science Foundation of China,No. 30772838
文摘This study established the following groups of rats： a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a semen coicis group. In all but the normal and sham surgery groups the left half of Tlo was transected. Four hours after model induction, 5-Hz and 100-Hz electroacupuncture were used to stimulate the acupoints I-luantiao （GB 30）, Zusan/i （ST 36）, Zhiyan9 （DU 9） and Xuanshu （DU 5）, or crude extract from semen coicis was intraperitoneally injected, for 8 consecutive weeks. The results indicated that electroacupuncture stimulation and intraperitoneal injection of semen coicis improved the morphology of spinal cord tissue, promoted the recovery of motion-evoked potentials, suppressed glial fibrillary acidic protein expression, and ameliorated motor function in rats with hemisection spinal cord injury. The effects of high-frequency （100 Hz） electroacupuncture ancl semen coicis were significant.
文摘Acupuncture,with its unique set of theories and its evident results in clinical practice is an important part of traditional Chinese medicine(TCM),and gaining recognition worldwide for the past few years.Although acupuncture treatment is generally accepted by the public,a few western researchers believe that sufficient evidence of its efficiency is still currently lacking,therefore creating a challenge of its acceptance on a wider scale.This article suggests the concepts of acupuncture behind clinical researches by reviewing the clinical researches done,analyzing the existing problems,and integrating real life acupuncture into the research process.By increasing the credibility of acupuncture,its reach can be extended globally and to more countries and regions.
基金sponsored by the Open Research Fund of Zhejiang First-foremost Key Subject-Acupuncture & Moxibustion,No. ZTK2010A07
文摘A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.
