Economic Management Professional Academic Education are increasingly becoming personalization,intelligence and application.Colleges and universities should actively use cloud computing and big data.Also Internet of Th...Economic Management Professional Academic Education are increasingly becoming personalization,intelligence and application.Colleges and universities should actively use cloud computing and big data.Also Internet of Things and other advanced information technologies to build an economics and management ERP virtual simulation experiment teaching platform.Cloud computing and big data,virtual simulation experiment teaching resources with"resource library+project library+enterprise management simulation sandbox training"as the core can build an online and offline collaborative and practical experiment teaching platform.It is expected to achieve the ideal effect of integration of three spaces.Such as physics and resources and social digital teaching.Moreover,it can also benefit human-computer collaboration and interactive teaching and inquiry learning.展开更多
Virtual simulation teaching is an addendum to the experimental teaching mode of railway engineering,and the two teaching methods complement each other and merge with each other.In view of the current research,there is...Virtual simulation teaching is an addendum to the experimental teaching mode of railway engineering,and the two teaching methods complement each other and merge with each other.In view of the current research,there is little discussion about the integration path of the two above.Based on the connotation and design of virtual simulation teaching,this research systematically expounds the integration of the real path and path optimization problems,and puts forward the railway engineering experimental teaching principles based on virtual simulation teaching.On the basis of this research,a virtual simulation experiment platform for vibration mechanics and its application in the floating slab vibration damping track was developed to make full use of three-dimensional modeling,virtual reality,human-computer interaction and other technologies,which can realistically simulate the vibration law and vibration damping effect of the rail transit system,and in the hope that the virtual simulation teaching can be widely used in the experimental teaching mode of railway engineering in the future.展开更多
Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of ma...Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of macromolecules,including proteins and nucleic acids.Moreover,it elucidates biological phenomena and principles at the molecular level,making it an essential foundational course for students pursuing various biology majors.As a foundational course for the basic application of aquaculture,Molecular Biology and Experiment requires guidance through numerous examples and cases.However,there are several challenges to address in developing the case library.Consequently,a case library has been established to meet the course requirements of Molecular Biology and Experiment for modern graduate students,with the central goal of reforming the educational model of higher education institutions and enhancing the effectiveness and quality of talent development.This strategy is designed to nurture highly skilled professionals who can address the current needs of the industry.展开更多
Heterogeneous metal-catalyzed chemical conversions with a recyclable catalyst are very ideal and challenging for sustainable organic synthesis.A new bipyridyl-Mo(IV)-carbon nitride(CN-K/Mo-Bpy)was prepared by supporti...Heterogeneous metal-catalyzed chemical conversions with a recyclable catalyst are very ideal and challenging for sustainable organic synthesis.A new bipyridyl-Mo(IV)-carbon nitride(CN-K/Mo-Bpy)was prepared by supporting molybdenum complex on C_(3)N_(4)-K and characterized by FT-IR,XRD,SEM,XPS and ICP-OES.Heterogeneous CN–Mo-Bpy catalyst can be applied to the direct amination of nitroarenes and arylboronic acid,thus constructing various valuable diarylamines in high to excellent yields with a wide substrate scope and good functional group tolerance.It is worth noting that this heterogeneous catalyst has high chemical stability and can be recycled for at least five times without reducing its activity.展开更多
This study constructs a reflective feedback model based on a pedagogical agent(PA)and explores its impact on students’problem-solving ability and cognitive load.A quasi-experimental design was used in the study,with ...This study constructs a reflective feedback model based on a pedagogical agent(PA)and explores its impact on students’problem-solving ability and cognitive load.A quasi-experimental design was used in the study,with 84 students from a middle school selected as the research subjects(44 in the experimental group and 40 in the control group).The experimental group used the reflective feedback model,while the control group used the factual feedback model.The results show that,compared with factual feedback,the reflective feedback model based on the pedagogical agent significantly improves students’problem-solving ability,especially at the action and thinking levels.In addition,this model effectively reduces students’cognitive load,especially in terms of internal and external load.展开更多
The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economic...The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economical and effective treatment methods.MicroRNA plays an important regulatory role in the occurrence and development of diseases.This study aims to explore the role and regulatory mechanism of miR⁃142a⁃3p in adriamycin(ADR)⁃induced renal tubular epithelial cell(TCMK⁃1)injury,with a focus on its potential as a therapeutic target for ADR nephropathy.First,cell viability was assessed using the CCK⁃8 kit,and a mouse renal tubular epithelial cell model induced by ADR was established.Subsequently,alterations in miR⁃142a⁃3p and its target gene ATG16L1 mRNA levels were quantified using RT⁃qPCR.Western blotting was used to detect the protein levels of autophagy marker proteins and pyroptosis marker proteins.Monodansylcadaverin(MDC)staining was performed and the autophagy of cells was detected by flow cytometry.The results showed that the relative expression of miR⁃142a⁃3p in TCMK⁃1 cells induced by ADR was increased and the relative expression of its target gene ATG16L1 was decreased(P<0.0001).Western blotting results showed that the levels of p62(P<0.001)and pyroptosis⁃related proteins(P<0.001)were increased,while the protein levels of autophagy⁃related proteins were decreased(P<0.05).The flow cytometry results showed that there was no difference in the mean fluorescence intensity of autoph⁃agosomes between the ADR group and the autophagosome inhibitor group(3⁃MA group)(P>0.05),indicating that after ADR induction,cell autophagy was inhibited and pyroptosis was enhanced.When the expression of miR⁃142a⁃3p was inhibited by transfecting miR⁃142a⁃3p inhibitor,the relative expression level of the target gene ATG16L1 was restored(P<0.001).Western blotting showed that the protein level of p62(P<0.01)and pyroptosis⁃related proteins(P<0.01)were decreased,and the protein level of autophagy⁃related proteins was restored(P<0.001).Flow cytometry results further indicated that cell autophagy was restored(P<0.0001).In conclusion,ADR targets ATG16L1 through miR⁃142a⁃3p to reduce the autophagy level of TCMK⁃1,and simultaneously activates GSDMD⁃mediated pyroptosis.展开更多
Background and Objective Social anxiety arising from intensive social media usage(SMU)among adolescents and youth has gained extensive attention in recent years due to its negative influence on mental health and acade...Background and Objective Social anxiety arising from intensive social media usage(SMU)among adolescents and youth has gained extensive attention in recent years due to its negative influence on mental health and academic performance.In spite of that,there is a dearth regarding the etiology of SMU-related social anxiety.This study aims to further clarify the influence of introversion personality on SMU-related social anxiety and the mechanism underlying such an association and provide a new perspective for developing effective intervention strategies for the highly prevailing SMU-related anxiety among Chinese college students.Methods A cohort of 979 college students(266 males and 713 females)aged 20.90±1.91 years was enrolled in this cross-sectional study.Four measures including the"extroversion"domain of Eysenck Personality Questionnaire Revised,Short Scale(EPQ-R-S E),Interaction Anxiousness Scale(IAS),Mobile Phone Addiction Index(MPAI),and Social Anxiety Scale for Social Media Users(SAS-SMU)were used to evaluate the influence of introversion personality on SMU-related social anxiety that was potentially mediated sequentially by interaction anxiousness and mobile phone addiction.Hayes PROCESS was used for correlation and mediation analysis.Results Interaction anxiousness(indirect effect=-1.331,95%CI:-1.559--1.122)partially mediated the association between introversion personality and SMU-related social anxiety.Besides,a sequential mediation of interaction anxiousness and mobile phone addiction in the link between introversion personality and SMU-related social anxiety was revealed(indirect effect=-0.308,95%CI:-0.404--0.220).No significant mediating effect was found with mobile phone addiction in the association between introversion personality and SMU-related social anxiety.Conclusion Targeting interaction anxiousness and mobile phone addiction may represent an efficient strategy alleviating SMU-related social anxiety among Chinese college students with introversion personality.展开更多
This study aims to optimize the use of lacquer residue biomass(LBM).We investigated the ability of LBM to remove Pb^(2+)heavy metal ions and the typical cationic dye methylene blue(MB)and anionic dye Congo red(CR)by s...This study aims to optimize the use of lacquer residue biomass(LBM).We investigated the ability of LBM to remove Pb^(2+)heavy metal ions and the typical cationic dye methylene blue(MB)and anionic dye Congo red(CR)by simultaneous adsorption from composite systems,as well as the relevant factors.Scanning electron microscopy(SEM),X-ray diffraction(XRD),and Fourier transform infrared spectroscopy(FTIR)were used to characterize adsorption behavior.The adsorption kinetics of Pb^(2+)-MB/CR composite systems can be effectively characterized by the pseudo-second-order kinetic model(R^(2)>0.97).In the Pb^(2+)-MB composite system,adsorption was antagonistic with similar adsorption sites.However,in the Pb^(2+)-CR composite system,we found that adsorption was synergistic with different adsorption sites,which led to a higher simultaneous adsorption capacity for a higher initial Pb^(2+)-CR concentration,unlike the Pb^(2+)-MB system.In both composite systems,an appropriate increase in LBM dosage and system temperature within a certain range was conducive to simultaneous adsorption and removal of Pb^(2+)-MB/CR composite systems.The optimal solid-liquid ratio and temperature were 1:75 and 30℃,respectively.The adsorption and removal rates of Pb^(2+)and MB were 99.98%and 90.49%,respectively,and those of Pb^(2+)and CR were 93.99%and 77.39%,respectively,in(50,50)mg/L of Pb^(2+)-MB/CR composite systems under these conditions.Adsorption removal of Pb^(2+)and MB improved with higher pH levels,and worsened with the increase of ionic strength in the solution,while the removal rate of CR showed an opposite trend.The coexisting anion and cation types had limited influence on the simultaneous adsorption removal of Pb^(2+),MB,and CR.The results of desorption showed that LBM can be utilized as a disposable material for simultaneously treating Pb^(2+)-MB/CR composite systems.The simultaneous adsorption mechanisms of Pb^(2+)-MB/CR mainly involved hydrogen bonding,π-πbonding interaction,and electrostatic interaction.展开更多
The remaining useful life prediction of rolling bearing is vital in safety and reliability guarantee.In engineering scenarios,only a small amount of bearing performance degradation data can be obtained through acceler...The remaining useful life prediction of rolling bearing is vital in safety and reliability guarantee.In engineering scenarios,only a small amount of bearing performance degradation data can be obtained through accelerated life testing.In the absence of lifetime data,the hidden long-term correlation between performance degradation data is challenging to mine effectively,which is the main factor that restricts the prediction precision and engineering application of the residual life prediction method.To address this problem,a novel method based on the multi-layer perception neural network and bidirectional long short-term memory network is proposed.Firstly,a nonlinear health indicator(HI)calculation method based on kernel principal component analysis(KPCA)and exponential weighted moving average(EWMA)is designed.Then,using the raw vibration data and HI,a multi-layer perceptron(MLP)neural network is trained to further calculate the HI of the online bearing in real time.Furthermore,The bidirectional long short-term memory model(BiLSTM)optimized by particle swarm optimization(PSO)is used to mine the time series features of HI and predict the remaining service life.Performance verification experiments and comparative experiments are carried out on the XJTU-SY bearing open dataset.The research results indicate that this method has an excellent ability to predict future HI and remaining life.展开更多
The purpose of this research is to analyze the causal mechanisms of learning difficulties of middle school students and use them to propose strategies to help them.This research is particularly valuable for its focus ...The purpose of this research is to analyze the causal mechanisms of learning difficulties of middle school students and use them to propose strategies to help them.This research is particularly valuable for its focus on middle school students.Research on this critical transition period is often lacking compared to primary and high school.Therefore,this research establishes a structured equation model and analyzes the data from the survey using the partial least squares method.The data were obtained from a 13,900 Wenzhou City,China students’questionnaire.The research found that learning strategies were the most significant influences on learning effectiveness,followed by learning motivation and learning relationships.Meanwhile,learning relationships had a significant impact on learning pressure.Therefore,this research proposes targeted support strategies.It aims to enhance learning motivation(Set achievable learning goals for each student with learning difficulties based on their actual situation),optimize learning strategies(Encourage students with learning difficulties to learn self-regulatory strategies such as goal setting,time management,and self-reflection),and improve learning relationships(Establish a good social network to promote positive interaction between students with learning difficulties and their peers).At the same time,it reduces students’learning pressure.Ultimately,the learning effectiveness of students with learning difficulties is improved.展开更多
Solar-driven photocatalytic hydrogen production via water splitting is considered as one of the most promising green and sustainable strategies,with the potential to replace traditional fossil fuels[1,2].Generally,thi...Solar-driven photocatalytic hydrogen production via water splitting is considered as one of the most promising green and sustainable strategies,with the potential to replace traditional fossil fuels[1,2].Generally,this photocatalytic reaction process includes the following steps:First,the semiconductor photocatalyst is photoexcited to generate photoinduced excitons on a femtosecond timescale.Next,the photoinduced excitons are separated into photogenerated electrons and holes,occurring within a femtosecond to picosecond timescale.Subsequently,only a small fraction of the photogenerated electrons and holes can overcome kinetic barriers,such as phonon scattering and bulk defects,to migrate to the surface。展开更多
As the core component of inertial navigation systems, fiber optic gyroscope (FOG), with technical advantages such as low power consumption, long lifespan, fast startup speed, and flexible structural design, are widely...As the core component of inertial navigation systems, fiber optic gyroscope (FOG), with technical advantages such as low power consumption, long lifespan, fast startup speed, and flexible structural design, are widely used in aerospace, unmanned driving, and other fields. However, due to the temper-ature sensitivity of optical devices, the influence of environmen-tal temperature causes errors in FOG, thereby greatly limiting their output accuracy. This work researches on machine-learn-ing based temperature error compensation techniques for FOG. Specifically, it focuses on compensating for the bias errors gen-erated in the fiber ring due to the Shupe effect. This work pro-poses a composite model based on k-means clustering, sup-port vector regression, and particle swarm optimization algo-rithms. And it significantly reduced redundancy within the sam-ples by adopting the interval sequence sample. Moreover, met-rics such as root mean square error (RMSE), mean absolute error (MAE), bias stability, and Allan variance, are selected to evaluate the model’s performance and compensation effective-ness. This work effectively enhances the consistency between data and models across different temperature ranges and tem-perature gradients, improving the bias stability of the FOG from 0.022 °/h to 0.006 °/h. Compared to the existing methods utiliz-ing a single machine learning model, the proposed method increases the bias stability of the compensated FOG from 57.11% to 71.98%, and enhances the suppression of rate ramp noise coefficient from 2.29% to 14.83%. This work improves the accuracy of FOG after compensation, providing theoretical guid-ance and technical references for sensors error compensation work in other fields.展开更多
Quasi-solid polymer electrolytes(QSPEs)have been attracted significant attentions due to their benefits for simultaneously improved safety and energy density of batteries.Developing electrolytes capable of forming a s...Quasi-solid polymer electrolytes(QSPEs)have been attracted significant attentions due to their benefits for simultaneously improved safety and energy density of batteries.Developing electrolytes capable of forming a stable solid electrolyte interphase(SEI)layer is a great challenge for QSPE-based lithium(Li)metal batteries(LMBs).Herein,unlike previously reports that the reconstruction of Li^(+)solvation structures in QSPE requires time-consuming bottom-up polymer synthesis,in current study,a facile approach has been developed to reconstruct the Li^(+)solvation structures in QSPE by adjustment of the salt concentrations.The high proportion of Li^(+)-anion complexes can effectively accelerate interfacial Li^(+)diffusion,mitigate the decompositions of organic solvents and induce the formation of a LiF-rich SEI layer,contributing to suppressed Li-dendrite growth.As a result,the Li/QSPE-3/LiFePO_(4)(LFP)cell performs an ultralong lifespan with capacity retention of 77.4%over 3000 cycles at 1 C.With a high-voltage LiCoO_(2)cathode,the cell can stably cycle over 200 cycles at 25℃(capacity retention of∼83.8%).With accelerated ion transport dynamics due to the reconstructed Li^(+)solvation structure,the QSPE-3(the salt concentration is 3 M)is applicable in a wide temperature range.The Li/QSPE-3/LFP full cell exhibits 58.1%and 102.6%of discharge capacity at−15 and 90℃,respectively,compared to those operated at 25℃This study demonstrates a facile yet effective approach on enhancing electrode/electrolyte interfacial stability,enabling the LMBs with simultaneously enhanced safety and high energy density.展开更多
AIM:To propose a novel classification system for late postoperative capsular block syndrome(CBS)based on the turbidity of intracapsular fluid,and to investigate the imaging and refractive changes before and after Nd:Y...AIM:To propose a novel classification system for late postoperative capsular block syndrome(CBS)based on the turbidity of intracapsular fluid,and to investigate the imaging and refractive changes before and after Nd:YAG laser posterior capsulotomy for each subtype.METHODS:A retrospective analysis was performed on 5 eyes from 5 patients with late postoperative CBS.Patients were categorized into turbid(3 eyes)or clear(2 eyes)types based on the turbidity of intracapsular fluid.Uncorrected visual acuity(UCVA),best-corrected visual acuity(BCVA),refractive status,slit-lamp images,Pentacam Scheimpflug data,and ultrasound biomicroscopy(UBM)images were evaluated before and 1mo after successful Nd:YAG laser posterior capsulotomy.RESULTS:Nd:YAG laser posterior capsulotomy significantly improved UCVA and BCVA in all 5 late CBS eyes.Pentacam imaging:turbid intracapsular fluid showed hyperreflection between posterior capsule and intraocular lens(IOL);clear fluid showed hyporeflection.UBM:posterior capsule was clear in turbid type but poorly defined in clear type.Capsulotomy increased aqueous depth only in clear type.Refractive changes:turbid fluid induced myopic shift.Clear type myopic shift was due to anterior IOL displacement and clear fluid-induced concave lens effect.CONCLUSION:Nd:YAG laser posterior capsulotomy is effective for late CBS.Turbid and clear late CBS types differ in imaging(Pentacam/UBM)and refractive mechanisms,supporting the proposed classification’s clinical value.展开更多
Optimizing the interfacial quality of halide perovskites heterojunction to promote the photogenerated charge separation is of great significance in photocatalytic reactions.However,the delicately regulation of interfa...Optimizing the interfacial quality of halide perovskites heterojunction to promote the photogenerated charge separation is of great significance in photocatalytic reactions.However,the delicately regulation of interfacial structure and properties of halide perovskites hybrid is still a big challenge owing to the growth uncontrollability and incompatibility between different constituents.Here we use Bi OBr nanosheets as the start-template to in situ epitaxially grow Cs_(3)Bi_(2)Br_(9)nanosheets by“cosharing”Bi and Br atoms strategy for designing a 2D/2D Cs_(3)Bi_(2)Br_(9)/BiOBr heterojunction.Systematic studies show that the epitaxial heterojunction can optimize the synergistic effect of Bi OBr and Cs_(3)Bi_(2)Br_(9)via the formation of tight-contact interfaces,strong interfacial electronic coupling and charge redistribution,which can not only drive the Z-scheme charge transfer mechanism to greatly promote the spatial separation of electronhole pairs,but also modulate the interfacial electronic structure to facilitate the adsorption and activation of toluene molecules.The heterojunction exhibited 62.3 and 2.4-fold photoactivity improvement for toluene oxidation to benzaldehyde than parental Bi OBr and Cs_(3)Bi_(2)Br_(9),respectively.This study not only proposed a novel dual atom-bridge protocol to engineer high-quality perovskite heterojunctions,but also uncovered the potential of heterojunction in promoting electron-hole separation as well as the application in photocatalytic organic synthesis.展开更多
Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances...Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances.Therefore,we obtained 2 peptides from salted egg white:Val-Val-His-Phe(VF-4)and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg(DR-8).The sodium dextran sulfate(DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo.The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis,attenuated colon tissue damage,inhibited the activation of nuclear factor kappa-B(NF-κB)/mitogen-activated protein kinase(MAPK)/phosphoinositide 3-kinase-Akt(PI3K-AKT)signaling pathways,and inhibited the expression of inflammatory cytokines.16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus,Blautia,and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter,Lachnospiraceae_NK4A136_group,Klebsiella.Moreover,the degree of correlation between pro-inflammatory cytokines and microbiota was as follows:interleukin-6(IL-6)>tumor necrosis factor-α(TNF-α)>interleukin-1β(IL-1β)>interferon-γ(IFN-γ).In conclusion,this study suggests that salted egg white peptides VF-4 and DR-8 have a significant antiinflammatory effect in vivo.It also provides a strategy for the treatment of IBD and a new way for the highvalue utilization of salted egg white.展开更多
Objective To investigate the chemical constituents and biological activities of soft coral Lemnalia sp.from the South China Sea.Methods The chemical constituents were isolated and purified by various chromatographic t...Objective To investigate the chemical constituents and biological activities of soft coral Lemnalia sp.from the South China Sea.Methods The chemical constituents were isolated and purified by various chromatographic techniques.The structures of all compounds were elucidated by using the modern spectrum analysis methods and comparison with the literature data.The antibacterial,immunosuppressive,and enzyme inhibitory activities of these compounds were evaluated.Results A new cembranoid diterpene,lemnaliaterpenoid A(1),and three known compounds,sinulaparvalide A(2),dihydrosinuflexolide(3),and capillolide(4)were isolated from the soft coral Lemnalia sp..Compound 1 showed antibacterial activity against Staphylococcus aureus and Escherichia coli with minimal inhibitory concentration(MIC)values of 16,16μg/mL,respectively.Compounds 1−3 displayed immunosuppressive activity against ConA-induced T cell(Jurkat cell)proliferation with inhibitory rates of 64.0%,67.8%,and 49.4%,respectively,at a concentration of 5μg/mL.Compounds 1−4 did not demonstrate enzyme inhibitory activity against the aldose reductase.Conclusion Four diterpenoid compounds were isolated from the soft coral Lemnalia sp.derived from the South China Sea,including one new cembranoid diterpene.Among them,the new compound 1 exhibitsed antibacterial and cytotoxic activities.The soft coral Lemnalia sp.from the South China Sea were prolific resources that produced structurally novel and biologically active secondary metabolites.展开更多
Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and...Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and metabolites.This study aimed to investigate how cold storage affects enzyme activities,nutrient composition,tissue microstructures and spoilage metabolites of bigeye tuna.The activities of cathepsins B,H,L increased,while Na^(+)/K^(+)-ATPase and Mg^(2+)-ATPase decreased,α-glucosidase,lipase and lipoxygenase first increased and then decreased during cold storage,suggesting that proteins undergo degradation and ATP metabolism occurs at a faster rate during cold storage.Nutrient composition(moisture and lipid content),total amino acids decreased,suggesting that the nutritional value of bigeye tuna was reduced.Besides,a logistic regression equation has been established as a food analysis tool and assesses the dynamics and correlation of the enzyme of bigeye tuna during cold storage.Based on untargeted metabolomic profiling analysis,a total of 524 metabolites were identified in the bigeye tuna contained several spoilage metabolites involved in lipid metabolism(glycerophosphocholine and choline phosphate),amino acid metabolism(L-histidine,5-deoxy-5′-(methylthio)adenosine,5-methylthioadenosine),carbohydrate metabolism(D-gluconic acid,α-D-fructose 1,6-bisphosphate,D-glyceraldehyde 3-phosphate).The results of tissue microstructures of tuna showed a looser network and visible deterioration of tissue fiber during cold storage.Therefore,metabolomic analysis and tissue microstructures provide insight into the spoilage mechanism investigations on bigeye tuna during cold storage.展开更多
This research study focuses on addressing the limitations of current neuropathic pain(NP)treatments by developing a novel dual-target modulator,E0199,targeting both Na_(V)1.7,Na_(V)1.8,and Na_(V)1.9 and K_(V)7 channel...This research study focuses on addressing the limitations of current neuropathic pain(NP)treatments by developing a novel dual-target modulator,E0199,targeting both Na_(V)1.7,Na_(V)1.8,and Na_(V)1.9 and K_(V)7 channels,a crucial regulator in controlling NP symptoms.The objective of the study was to synthesize a compound capable of modulating these channels to alleviate NP.Through an experimental design involving both in vitro and in vivo methods,E0199 was tested for its efficacy on ion channels and its therapeutic potential in a chronic constriction injury(CCI)mouse model.The results demonstrated that E0199 significantly inhibited Na_(V)1.7,Na_(V)1.8,and Na_(V)1.9 channels with a particularly low half maximal inhibitory concentration(IC50)for Na_(V)1.9 by promoting sodium channel inactivation,and also effectively increased K_(V)7.2/7.3,K_(V)7.2,and K_(V)7.5 channels,excluding K_(V)7.1 by promoting potassium channel activation.This dual action significantly reduced the excitability of dorsal root ganglion neurons and alleviated pain hypersensitivity in mice at low doses,indicating a potent analgesic effect without affecting heart and skeletal muscle ion channels critically.The safety of E0199 was supported by neurobehavioral evaluations.Conclusively,E0199 represents a ground-breaking approach in NP treatment,showcasing the potential of dual-target small-molecule compounds in providing a more effective and safe therapeutic option for NP.This study introduces a promising direction for the future development of NP therapeutics.展开更多
Pharmaceutical comprehensive study(PCS) is a new system of experimental teaching in China, which integrates multidisciplinary pharmaceutical knowledge and covers the basic process of new drug discovery. To explore the...Pharmaceutical comprehensive study(PCS) is a new system of experimental teaching in China, which integrates multidisciplinary pharmaceutical knowledge and covers the basic process of new drug discovery. To explore the feasibility of this experiment teaching system and mode, we developed PCS as an elective course. The PCS is designed with two sections: pharmaceutical comprehensive design(PCD) and pharmaceutical comprehensive experiment(PCE). The PCD section includes literature review, comprehensive project design and oral examination. PCE can be divided into four parts: synthesis, quantitative determination, pharmacodynamic evaluation, and formulation and quality determination. Course grade was determined by experimental performance, written report, literature review, new project design and oral examination. The learning interest, experimental ability, theoretical level and literature retrieval ability, team spirit and interpersonal skills have been all significantly improved among students taking this course. A survey was administered at the end of the semester to the enrolled students. The responses were reported as percentages, and the feedback was positive. The course was highly recommended by the teaching inspection committee. This new course plays an important role in developing students’ creativity and comprehensive ability. It could help students understand the focus and features of every secondary discipline, as well as establish scientific and reasonable knowledge system. Most students can better understand the process of drug research after this course.展开更多
基金the 2020 University Innovation and Entrepreneurship Project of Guangdong University of Foreign Studies.
文摘Economic Management Professional Academic Education are increasingly becoming personalization,intelligence and application.Colleges and universities should actively use cloud computing and big data.Also Internet of Things and other advanced information technologies to build an economics and management ERP virtual simulation experiment teaching platform.Cloud computing and big data,virtual simulation experiment teaching resources with"resource library+project library+enterprise management simulation sandbox training"as the core can build an online and offline collaborative and practical experiment teaching platform.It is expected to achieve the ideal effect of integration of three spaces.Such as physics and resources and social digital teaching.Moreover,it can also benefit human-computer collaboration and interactive teaching and inquiry learning.
基金The research is financially supported by First-class Undergraduate Course Funding Project in Hunan Province-Virtual Simulation Experimental Teaching Course(Xiang Jiao Tong[2021]No.28),Hunan Engineering Teaching Team)(Xiang Jiao Tong[2019]No.370)National Experimental Teaching Center of Civil Engineering Virtual Simulation(Central South University)Open Project(202001)Education and Teaching Reform Project of Central South University(2020jy063),which is gratefully acknowledged by the authors.
文摘Virtual simulation teaching is an addendum to the experimental teaching mode of railway engineering,and the two teaching methods complement each other and merge with each other.In view of the current research,there is little discussion about the integration path of the two above.Based on the connotation and design of virtual simulation teaching,this research systematically expounds the integration of the real path and path optimization problems,and puts forward the railway engineering experimental teaching principles based on virtual simulation teaching.On the basis of this research,a virtual simulation experiment platform for vibration mechanics and its application in the floating slab vibration damping track was developed to make full use of three-dimensional modeling,virtual reality,human-computer interaction and other technologies,which can realistically simulate the vibration law and vibration damping effect of the rail transit system,and in the hope that the virtual simulation teaching can be widely used in the experimental teaching mode of railway engineering in the future.
基金Supported by Degree and Graduate Student Education Reform Research Project of Guangdong Ocean University(202315,202416).
文摘Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of macromolecules,including proteins and nucleic acids.Moreover,it elucidates biological phenomena and principles at the molecular level,making it an essential foundational course for students pursuing various biology majors.As a foundational course for the basic application of aquaculture,Molecular Biology and Experiment requires guidance through numerous examples and cases.However,there are several challenges to address in developing the case library.Consequently,a case library has been established to meet the course requirements of Molecular Biology and Experiment for modern graduate students,with the central goal of reforming the educational model of higher education institutions and enhancing the effectiveness and quality of talent development.This strategy is designed to nurture highly skilled professionals who can address the current needs of the industry.
基金support for this work by Hebei Education Department(No.JZX2024004)Central Guidance on Local Science and Technology Development Fund of Hebei Province(No.236Z1404G)+3 种基金the National Natural Science Foundation of China(Nos.22301060 and 21272053)China Postdoctoral Science Foundation(No.2023M730914)the Natural Science Foundation of Hebei Province(Biopharmaceutical Joint Fund No.B2022206008)Project of Science and Technology Department of Hebei Province(No.22567622H)。
文摘Heterogeneous metal-catalyzed chemical conversions with a recyclable catalyst are very ideal and challenging for sustainable organic synthesis.A new bipyridyl-Mo(IV)-carbon nitride(CN-K/Mo-Bpy)was prepared by supporting molybdenum complex on C_(3)N_(4)-K and characterized by FT-IR,XRD,SEM,XPS and ICP-OES.Heterogeneous CN–Mo-Bpy catalyst can be applied to the direct amination of nitroarenes and arylboronic acid,thus constructing various valuable diarylamines in high to excellent yields with a wide substrate scope and good functional group tolerance.It is worth noting that this heterogeneous catalyst has high chemical stability and can be recycled for at least five times without reducing its activity.
基金023 Zhejiang Provincial Department of Education General Project:Research on an interdisciplinary teaching model to promote the development of computational thinking in the context of the new curriculum standards[Grant NO:Y202351596]Key Project of Zhejiang Provincial Education Science Planning:Research on an interdisciplinary teaching model to promote students’computational thinking from multiple analytical perspectives[Grant NO:2025SB103].
文摘This study constructs a reflective feedback model based on a pedagogical agent(PA)and explores its impact on students’problem-solving ability and cognitive load.A quasi-experimental design was used in the study,with 84 students from a middle school selected as the research subjects(44 in the experimental group and 40 in the control group).The experimental group used the reflective feedback model,while the control group used the factual feedback model.The results show that,compared with factual feedback,the reflective feedback model based on the pedagogical agent significantly improves students’problem-solving ability,especially at the action and thinking levels.In addition,this model effectively reduces students’cognitive load,especially in terms of internal and external load.
文摘The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economical and effective treatment methods.MicroRNA plays an important regulatory role in the occurrence and development of diseases.This study aims to explore the role and regulatory mechanism of miR⁃142a⁃3p in adriamycin(ADR)⁃induced renal tubular epithelial cell(TCMK⁃1)injury,with a focus on its potential as a therapeutic target for ADR nephropathy.First,cell viability was assessed using the CCK⁃8 kit,and a mouse renal tubular epithelial cell model induced by ADR was established.Subsequently,alterations in miR⁃142a⁃3p and its target gene ATG16L1 mRNA levels were quantified using RT⁃qPCR.Western blotting was used to detect the protein levels of autophagy marker proteins and pyroptosis marker proteins.Monodansylcadaverin(MDC)staining was performed and the autophagy of cells was detected by flow cytometry.The results showed that the relative expression of miR⁃142a⁃3p in TCMK⁃1 cells induced by ADR was increased and the relative expression of its target gene ATG16L1 was decreased(P<0.0001).Western blotting results showed that the levels of p62(P<0.001)and pyroptosis⁃related proteins(P<0.001)were increased,while the protein levels of autophagy⁃related proteins were decreased(P<0.05).The flow cytometry results showed that there was no difference in the mean fluorescence intensity of autoph⁃agosomes between the ADR group and the autophagosome inhibitor group(3⁃MA group)(P>0.05),indicating that after ADR induction,cell autophagy was inhibited and pyroptosis was enhanced.When the expression of miR⁃142a⁃3p was inhibited by transfecting miR⁃142a⁃3p inhibitor,the relative expression level of the target gene ATG16L1 was restored(P<0.001).Western blotting showed that the protein level of p62(P<0.01)and pyroptosis⁃related proteins(P<0.01)were decreased,and the protein level of autophagy⁃related proteins was restored(P<0.001).Flow cytometry results further indicated that cell autophagy was restored(P<0.0001).In conclusion,ADR targets ATG16L1 through miR⁃142a⁃3p to reduce the autophagy level of TCMK⁃1,and simultaneously activates GSDMD⁃mediated pyroptosis.
文摘Background and Objective Social anxiety arising from intensive social media usage(SMU)among adolescents and youth has gained extensive attention in recent years due to its negative influence on mental health and academic performance.In spite of that,there is a dearth regarding the etiology of SMU-related social anxiety.This study aims to further clarify the influence of introversion personality on SMU-related social anxiety and the mechanism underlying such an association and provide a new perspective for developing effective intervention strategies for the highly prevailing SMU-related anxiety among Chinese college students.Methods A cohort of 979 college students(266 males and 713 females)aged 20.90±1.91 years was enrolled in this cross-sectional study.Four measures including the"extroversion"domain of Eysenck Personality Questionnaire Revised,Short Scale(EPQ-R-S E),Interaction Anxiousness Scale(IAS),Mobile Phone Addiction Index(MPAI),and Social Anxiety Scale for Social Media Users(SAS-SMU)were used to evaluate the influence of introversion personality on SMU-related social anxiety that was potentially mediated sequentially by interaction anxiousness and mobile phone addiction.Hayes PROCESS was used for correlation and mediation analysis.Results Interaction anxiousness(indirect effect=-1.331,95%CI:-1.559--1.122)partially mediated the association between introversion personality and SMU-related social anxiety.Besides,a sequential mediation of interaction anxiousness and mobile phone addiction in the link between introversion personality and SMU-related social anxiety was revealed(indirect effect=-0.308,95%CI:-0.404--0.220).No significant mediating effect was found with mobile phone addiction in the association between introversion personality and SMU-related social anxiety.Conclusion Targeting interaction anxiousness and mobile phone addiction may represent an efficient strategy alleviating SMU-related social anxiety among Chinese college students with introversion personality.
基金National Key Research and Development Program of China(Nos.2023YFD1702003 and 2023YFC3709001).
文摘This study aims to optimize the use of lacquer residue biomass(LBM).We investigated the ability of LBM to remove Pb^(2+)heavy metal ions and the typical cationic dye methylene blue(MB)and anionic dye Congo red(CR)by simultaneous adsorption from composite systems,as well as the relevant factors.Scanning electron microscopy(SEM),X-ray diffraction(XRD),and Fourier transform infrared spectroscopy(FTIR)were used to characterize adsorption behavior.The adsorption kinetics of Pb^(2+)-MB/CR composite systems can be effectively characterized by the pseudo-second-order kinetic model(R^(2)>0.97).In the Pb^(2+)-MB composite system,adsorption was antagonistic with similar adsorption sites.However,in the Pb^(2+)-CR composite system,we found that adsorption was synergistic with different adsorption sites,which led to a higher simultaneous adsorption capacity for a higher initial Pb^(2+)-CR concentration,unlike the Pb^(2+)-MB system.In both composite systems,an appropriate increase in LBM dosage and system temperature within a certain range was conducive to simultaneous adsorption and removal of Pb^(2+)-MB/CR composite systems.The optimal solid-liquid ratio and temperature were 1:75 and 30℃,respectively.The adsorption and removal rates of Pb^(2+)and MB were 99.98%and 90.49%,respectively,and those of Pb^(2+)and CR were 93.99%and 77.39%,respectively,in(50,50)mg/L of Pb^(2+)-MB/CR composite systems under these conditions.Adsorption removal of Pb^(2+)and MB improved with higher pH levels,and worsened with the increase of ionic strength in the solution,while the removal rate of CR showed an opposite trend.The coexisting anion and cation types had limited influence on the simultaneous adsorption removal of Pb^(2+),MB,and CR.The results of desorption showed that LBM can be utilized as a disposable material for simultaneously treating Pb^(2+)-MB/CR composite systems.The simultaneous adsorption mechanisms of Pb^(2+)-MB/CR mainly involved hydrogen bonding,π-πbonding interaction,and electrostatic interaction.
基金supported by the National Key Research and Development Project(Grant Number 2023YFB3709601)the National Natural Science Foundation of China(Grant Numbers 62373215,62373219,62073193)+2 种基金the Key Research and Development Plan of Shandong Province(Grant Numbers 2021CXGC010204,2022CXGC020902)the Fundamental Research Funds of Shandong University(Grant Number 2021JCG008)the Natural Science Foundation of Shandong Province(Grant Number ZR2023MF100).
文摘The remaining useful life prediction of rolling bearing is vital in safety and reliability guarantee.In engineering scenarios,only a small amount of bearing performance degradation data can be obtained through accelerated life testing.In the absence of lifetime data,the hidden long-term correlation between performance degradation data is challenging to mine effectively,which is the main factor that restricts the prediction precision and engineering application of the residual life prediction method.To address this problem,a novel method based on the multi-layer perception neural network and bidirectional long short-term memory network is proposed.Firstly,a nonlinear health indicator(HI)calculation method based on kernel principal component analysis(KPCA)and exponential weighted moving average(EWMA)is designed.Then,using the raw vibration data and HI,a multi-layer perceptron(MLP)neural network is trained to further calculate the HI of the online bearing in real time.Furthermore,The bidirectional long short-term memory model(BiLSTM)optimized by particle swarm optimization(PSO)is used to mine the time series features of HI and predict the remaining service life.Performance verification experiments and comparative experiments are carried out on the XJTU-SY bearing open dataset.The research results indicate that this method has an excellent ability to predict future HI and remaining life.
基金2025 Wenzhou Key Research Base of Philosophy and Social Science(Wenzhou University Learning Science and Technology Research Centre)Research Project:Investigation and Strategy Research on the Causes of Middle School Students’Learning Difficulties in the Context of the Leading Country in Education.
文摘The purpose of this research is to analyze the causal mechanisms of learning difficulties of middle school students and use them to propose strategies to help them.This research is particularly valuable for its focus on middle school students.Research on this critical transition period is often lacking compared to primary and high school.Therefore,this research establishes a structured equation model and analyzes the data from the survey using the partial least squares method.The data were obtained from a 13,900 Wenzhou City,China students’questionnaire.The research found that learning strategies were the most significant influences on learning effectiveness,followed by learning motivation and learning relationships.Meanwhile,learning relationships had a significant impact on learning pressure.Therefore,this research proposes targeted support strategies.It aims to enhance learning motivation(Set achievable learning goals for each student with learning difficulties based on their actual situation),optimize learning strategies(Encourage students with learning difficulties to learn self-regulatory strategies such as goal setting,time management,and self-reflection),and improve learning relationships(Establish a good social network to promote positive interaction between students with learning difficulties and their peers).At the same time,it reduces students’learning pressure.Ultimately,the learning effectiveness of students with learning difficulties is improved.
文摘Solar-driven photocatalytic hydrogen production via water splitting is considered as one of the most promising green and sustainable strategies,with the potential to replace traditional fossil fuels[1,2].Generally,this photocatalytic reaction process includes the following steps:First,the semiconductor photocatalyst is photoexcited to generate photoinduced excitons on a femtosecond timescale.Next,the photoinduced excitons are separated into photogenerated electrons and holes,occurring within a femtosecond to picosecond timescale.Subsequently,only a small fraction of the photogenerated electrons and holes can overcome kinetic barriers,such as phonon scattering and bulk defects,to migrate to the surface。
基金supported by the National Natural Science Foundation of China(62375013).
文摘As the core component of inertial navigation systems, fiber optic gyroscope (FOG), with technical advantages such as low power consumption, long lifespan, fast startup speed, and flexible structural design, are widely used in aerospace, unmanned driving, and other fields. However, due to the temper-ature sensitivity of optical devices, the influence of environmen-tal temperature causes errors in FOG, thereby greatly limiting their output accuracy. This work researches on machine-learn-ing based temperature error compensation techniques for FOG. Specifically, it focuses on compensating for the bias errors gen-erated in the fiber ring due to the Shupe effect. This work pro-poses a composite model based on k-means clustering, sup-port vector regression, and particle swarm optimization algo-rithms. And it significantly reduced redundancy within the sam-ples by adopting the interval sequence sample. Moreover, met-rics such as root mean square error (RMSE), mean absolute error (MAE), bias stability, and Allan variance, are selected to evaluate the model’s performance and compensation effective-ness. This work effectively enhances the consistency between data and models across different temperature ranges and tem-perature gradients, improving the bias stability of the FOG from 0.022 °/h to 0.006 °/h. Compared to the existing methods utiliz-ing a single machine learning model, the proposed method increases the bias stability of the compensated FOG from 57.11% to 71.98%, and enhances the suppression of rate ramp noise coefficient from 2.29% to 14.83%. This work improves the accuracy of FOG after compensation, providing theoretical guid-ance and technical references for sensors error compensation work in other fields.
基金supported by the Natural Science Foundation of China(22379073,52373275)the Natural Science Foundation of Tianjin,China(18JCZDJC31400)the Ministry of Education Innovation Team(IRT13022).
文摘Quasi-solid polymer electrolytes(QSPEs)have been attracted significant attentions due to their benefits for simultaneously improved safety and energy density of batteries.Developing electrolytes capable of forming a stable solid electrolyte interphase(SEI)layer is a great challenge for QSPE-based lithium(Li)metal batteries(LMBs).Herein,unlike previously reports that the reconstruction of Li^(+)solvation structures in QSPE requires time-consuming bottom-up polymer synthesis,in current study,a facile approach has been developed to reconstruct the Li^(+)solvation structures in QSPE by adjustment of the salt concentrations.The high proportion of Li^(+)-anion complexes can effectively accelerate interfacial Li^(+)diffusion,mitigate the decompositions of organic solvents and induce the formation of a LiF-rich SEI layer,contributing to suppressed Li-dendrite growth.As a result,the Li/QSPE-3/LiFePO_(4)(LFP)cell performs an ultralong lifespan with capacity retention of 77.4%over 3000 cycles at 1 C.With a high-voltage LiCoO_(2)cathode,the cell can stably cycle over 200 cycles at 25℃(capacity retention of∼83.8%).With accelerated ion transport dynamics due to the reconstructed Li^(+)solvation structure,the QSPE-3(the salt concentration is 3 M)is applicable in a wide temperature range.The Li/QSPE-3/LFP full cell exhibits 58.1%and 102.6%of discharge capacity at−15 and 90℃,respectively,compared to those operated at 25℃This study demonstrates a facile yet effective approach on enhancing electrode/electrolyte interfacial stability,enabling the LMBs with simultaneously enhanced safety and high energy density.
基金Supported by the Research and Development Program of Shaanxi Province(No.2021SF-161)the Medical Research Project of Xi’an Science and Technology Action Plan[No.2019114613YX001SF041(1)]。
文摘AIM:To propose a novel classification system for late postoperative capsular block syndrome(CBS)based on the turbidity of intracapsular fluid,and to investigate the imaging and refractive changes before and after Nd:YAG laser posterior capsulotomy for each subtype.METHODS:A retrospective analysis was performed on 5 eyes from 5 patients with late postoperative CBS.Patients were categorized into turbid(3 eyes)or clear(2 eyes)types based on the turbidity of intracapsular fluid.Uncorrected visual acuity(UCVA),best-corrected visual acuity(BCVA),refractive status,slit-lamp images,Pentacam Scheimpflug data,and ultrasound biomicroscopy(UBM)images were evaluated before and 1mo after successful Nd:YAG laser posterior capsulotomy.RESULTS:Nd:YAG laser posterior capsulotomy significantly improved UCVA and BCVA in all 5 late CBS eyes.Pentacam imaging:turbid intracapsular fluid showed hyperreflection between posterior capsule and intraocular lens(IOL);clear fluid showed hyporeflection.UBM:posterior capsule was clear in turbid type but poorly defined in clear type.Capsulotomy increased aqueous depth only in clear type.Refractive changes:turbid fluid induced myopic shift.Clear type myopic shift was due to anterior IOL displacement and clear fluid-induced concave lens effect.CONCLUSION:Nd:YAG laser posterior capsulotomy is effective for late CBS.Turbid and clear late CBS types differ in imaging(Pentacam/UBM)and refractive mechanisms,supporting the proposed classification’s clinical value.
基金financially supported by the National Natural Science Foundation of China(Nos.22175202,22005351,22365016)the Guangdong Basic and Applied Basic Research Foundation(No.2023A1515010180)+3 种基金Program of Guangzhou Science and Technology(No.202201011591)the Science and Technology Innovation Program of Hunan Province(No.2023RC3179)Scientific Research Start-up Fund of Jishou University(No.1122003)support of National Supercomputer Center in Sun Yat-sen University,Guangzhou。
文摘Optimizing the interfacial quality of halide perovskites heterojunction to promote the photogenerated charge separation is of great significance in photocatalytic reactions.However,the delicately regulation of interfacial structure and properties of halide perovskites hybrid is still a big challenge owing to the growth uncontrollability and incompatibility between different constituents.Here we use Bi OBr nanosheets as the start-template to in situ epitaxially grow Cs_(3)Bi_(2)Br_(9)nanosheets by“cosharing”Bi and Br atoms strategy for designing a 2D/2D Cs_(3)Bi_(2)Br_(9)/BiOBr heterojunction.Systematic studies show that the epitaxial heterojunction can optimize the synergistic effect of Bi OBr and Cs_(3)Bi_(2)Br_(9)via the formation of tight-contact interfaces,strong interfacial electronic coupling and charge redistribution,which can not only drive the Z-scheme charge transfer mechanism to greatly promote the spatial separation of electronhole pairs,but also modulate the interfacial electronic structure to facilitate the adsorption and activation of toluene molecules.The heterojunction exhibited 62.3 and 2.4-fold photoactivity improvement for toluene oxidation to benzaldehyde than parental Bi OBr and Cs_(3)Bi_(2)Br_(9),respectively.This study not only proposed a novel dual atom-bridge protocol to engineer high-quality perovskite heterojunctions,but also uncovered the potential of heterojunction in promoting electron-hole separation as well as the application in photocatalytic organic synthesis.
基金the financial support provided by the Project of Jiangxi Provincial Department of Education,China(GJJ200433)。
文摘Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances.Therefore,we obtained 2 peptides from salted egg white:Val-Val-His-Phe(VF-4)and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg(DR-8).The sodium dextran sulfate(DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo.The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis,attenuated colon tissue damage,inhibited the activation of nuclear factor kappa-B(NF-κB)/mitogen-activated protein kinase(MAPK)/phosphoinositide 3-kinase-Akt(PI3K-AKT)signaling pathways,and inhibited the expression of inflammatory cytokines.16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus,Blautia,and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter,Lachnospiraceae_NK4A136_group,Klebsiella.Moreover,the degree of correlation between pro-inflammatory cytokines and microbiota was as follows:interleukin-6(IL-6)>tumor necrosis factor-α(TNF-α)>interleukin-1β(IL-1β)>interferon-γ(IFN-γ).In conclusion,this study suggests that salted egg white peptides VF-4 and DR-8 have a significant antiinflammatory effect in vivo.It also provides a strategy for the treatment of IBD and a new way for the highvalue utilization of salted egg white.
文摘Objective To investigate the chemical constituents and biological activities of soft coral Lemnalia sp.from the South China Sea.Methods The chemical constituents were isolated and purified by various chromatographic techniques.The structures of all compounds were elucidated by using the modern spectrum analysis methods and comparison with the literature data.The antibacterial,immunosuppressive,and enzyme inhibitory activities of these compounds were evaluated.Results A new cembranoid diterpene,lemnaliaterpenoid A(1),and three known compounds,sinulaparvalide A(2),dihydrosinuflexolide(3),and capillolide(4)were isolated from the soft coral Lemnalia sp..Compound 1 showed antibacterial activity against Staphylococcus aureus and Escherichia coli with minimal inhibitory concentration(MIC)values of 16,16μg/mL,respectively.Compounds 1−3 displayed immunosuppressive activity against ConA-induced T cell(Jurkat cell)proliferation with inhibitory rates of 64.0%,67.8%,and 49.4%,respectively,at a concentration of 5μg/mL.Compounds 1−4 did not demonstrate enzyme inhibitory activity against the aldose reductase.Conclusion Four diterpenoid compounds were isolated from the soft coral Lemnalia sp.derived from the South China Sea,including one new cembranoid diterpene.Among them,the new compound 1 exhibitsed antibacterial and cytotoxic activities.The soft coral Lemnalia sp.from the South China Sea were prolific resources that produced structurally novel and biologically active secondary metabolites.
基金supported by the Shanghai Sailing Program(22YF1416300)Youth Fund Project of National Natural Science Foundation of China(32202117)+1 种基金National Key Research and Development Program of China(2022YFD2100104)the China Agriculture Research System(CARS-47).
文摘Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage,however,there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and metabolites.This study aimed to investigate how cold storage affects enzyme activities,nutrient composition,tissue microstructures and spoilage metabolites of bigeye tuna.The activities of cathepsins B,H,L increased,while Na^(+)/K^(+)-ATPase and Mg^(2+)-ATPase decreased,α-glucosidase,lipase and lipoxygenase first increased and then decreased during cold storage,suggesting that proteins undergo degradation and ATP metabolism occurs at a faster rate during cold storage.Nutrient composition(moisture and lipid content),total amino acids decreased,suggesting that the nutritional value of bigeye tuna was reduced.Besides,a logistic regression equation has been established as a food analysis tool and assesses the dynamics and correlation of the enzyme of bigeye tuna during cold storage.Based on untargeted metabolomic profiling analysis,a total of 524 metabolites were identified in the bigeye tuna contained several spoilage metabolites involved in lipid metabolism(glycerophosphocholine and choline phosphate),amino acid metabolism(L-histidine,5-deoxy-5′-(methylthio)adenosine,5-methylthioadenosine),carbohydrate metabolism(D-gluconic acid,α-D-fructose 1,6-bisphosphate,D-glyceraldehyde 3-phosphate).The results of tissue microstructures of tuna showed a looser network and visible deterioration of tissue fiber during cold storage.Therefore,metabolomic analysis and tissue microstructures provide insight into the spoilage mechanism investigations on bigeye tuna during cold storage.
基金funded by the Key Project from the Hebei Provincial Department of Science and Technology,China(Grant No.:21372601D)the Foundation Postdoctoral Mobile Station of Basic Medical Sciences,Hebei Medical University,China(Grant No.:20123120019)+4 种基金the Natural Science Foundation of Hebei Province,China(Grant No.:H2021206352)the Science and Technology Research Project of Colleges and Universities in Hebei Province,China(Grant No.:QN2023197)Hebei Medical University,Science and Technology,China(Grant No.:CYQD2023014)Hebei Provincial Department of Human Resources and Social Security,China(Grant No.:B2023003034)the Consultative Foundation from Hebei Province,China(Grant No.:2020TXZH01).
文摘This research study focuses on addressing the limitations of current neuropathic pain(NP)treatments by developing a novel dual-target modulator,E0199,targeting both Na_(V)1.7,Na_(V)1.8,and Na_(V)1.9 and K_(V)7 channels,a crucial regulator in controlling NP symptoms.The objective of the study was to synthesize a compound capable of modulating these channels to alleviate NP.Through an experimental design involving both in vitro and in vivo methods,E0199 was tested for its efficacy on ion channels and its therapeutic potential in a chronic constriction injury(CCI)mouse model.The results demonstrated that E0199 significantly inhibited Na_(V)1.7,Na_(V)1.8,and Na_(V)1.9 channels with a particularly low half maximal inhibitory concentration(IC50)for Na_(V)1.9 by promoting sodium channel inactivation,and also effectively increased K_(V)7.2/7.3,K_(V)7.2,and K_(V)7.5 channels,excluding K_(V)7.1 by promoting potassium channel activation.This dual action significantly reduced the excitability of dorsal root ganglion neurons and alleviated pain hypersensitivity in mice at low doses,indicating a potent analgesic effect without affecting heart and skeletal muscle ion channels critically.The safety of E0199 was supported by neurobehavioral evaluations.Conclusively,E0199 represents a ground-breaking approach in NP treatment,showcasing the potential of dual-target small-molecule compounds in providing a more effective and safe therapeutic option for NP.This study introduces a promising direction for the future development of NP therapeutics.
基金The Education and Teaching Research Project of Peking University Health Science Center
文摘Pharmaceutical comprehensive study(PCS) is a new system of experimental teaching in China, which integrates multidisciplinary pharmaceutical knowledge and covers the basic process of new drug discovery. To explore the feasibility of this experiment teaching system and mode, we developed PCS as an elective course. The PCS is designed with two sections: pharmaceutical comprehensive design(PCD) and pharmaceutical comprehensive experiment(PCE). The PCD section includes literature review, comprehensive project design and oral examination. PCE can be divided into four parts: synthesis, quantitative determination, pharmacodynamic evaluation, and formulation and quality determination. Course grade was determined by experimental performance, written report, literature review, new project design and oral examination. The learning interest, experimental ability, theoretical level and literature retrieval ability, team spirit and interpersonal skills have been all significantly improved among students taking this course. A survey was administered at the end of the semester to the enrolled students. The responses were reported as percentages, and the feedback was positive. The course was highly recommended by the teaching inspection committee. This new course plays an important role in developing students’ creativity and comprehensive ability. It could help students understand the focus and features of every secondary discipline, as well as establish scientific and reasonable knowledge system. Most students can better understand the process of drug research after this course.
