The valorization of starch-rich materials typically requires high-temperature gelatinization prior to enzymatic saccharification to convert the starch into usable sugars,which are subsequently transformed into biochem...The valorization of starch-rich materials typically requires high-temperature gelatinization prior to enzymatic saccharification to convert the starch into usable sugars,which are subsequently transformed into biochemical products.To bypass the energy-intensive gelatinization step,effective thermostable enzymes capable of degrading raw starch are essential.In this work,a raw starch-digesting amylolytic enzyme system was produced by a novel isolate,Priestia koreensis HL12 using sago starch as a carbon source at 30◦C for 48 h.The crude enzyme demonstrated thermophilic properties,exhibiting robust amylase activity across a broad temperature range(30-100◦C),with the highest amylase activity(168.05 U/mg protein)at 65◦C in 50 mM sodium phos-phate buffer pH 6 toward soluble starch.The enzyme efficiently hydrolyzed both raw and gelatinized starches,predominantly yielding maltooligosaccharides(DP3 and DP5)as valuable prebiotics,without producing glucose.The highest sugar yield was obtained from gelatinized potato starch,with 841.58 mg/g substrate(84%con-version),while cassava pulp hydrolysis below the gelatinization temperature achieved a 57.32%conversion,producing 434.65 mg/g maltopentaose as majority equivalent to 75.8%of product mixture.This non-thermal saccharification process without glucose byproduct has significant potential for prebiotic production,adding value to starch-rich agricultural byproducts andcontributing to a sustainable,zero-waste starch industry.展开更多
Bacterial cellulose is a versatile material with applications in many industries. However, the widespread uptake of bacterial cellulose faces challenges including high production costs and lack of scalability. One app...Bacterial cellulose is a versatile material with applications in many industries. However, the widespread uptake of bacterial cellulose faces challenges including high production costs and lack of scalability. One approach to address these obstacles is the use of alternative substrates and media, compared to the Hestrin-Schramm (HS) media. By evaluating and selecting appro- priate media and substrates, the production of bacterial cellulose can be more efficient: enabling sustainable systems and supply chains where less energy and materials are lost, and the output production is increased. The purpose of this paper is to analyze the current landscape of bac- terial cellulose alternative media and substrates (ingredients). Through a systematic review of 198 papers, this review identifies 299 alternative substrates from 12 industries and 101 bacte- rial cellulose-producing strains, which were systematically compared. This review also finds that there are methodological gaps in this field such as data variability, papers mislabelling the HS media or not using a comparison media, and a lack of strain names. This alternative substrate analysis for bacterial cellulose production demonstrates that overall, for some applications al- ternative substrates can be taken into consideration that are not only cheaper, but also produce higher yields than HS media.展开更多
基金financially supported by Thammasat University Research Fund(Contract No.TUFT 4/2566)the 30st Science&Technology Research Grant from Thailand Toray Science Foundation.This work was also supported by the National Science,Research and Innovation Fund,Thailand Science Research and Innovation(TSRI)(Grant No.:FFB680075/0337).
文摘The valorization of starch-rich materials typically requires high-temperature gelatinization prior to enzymatic saccharification to convert the starch into usable sugars,which are subsequently transformed into biochemical products.To bypass the energy-intensive gelatinization step,effective thermostable enzymes capable of degrading raw starch are essential.In this work,a raw starch-digesting amylolytic enzyme system was produced by a novel isolate,Priestia koreensis HL12 using sago starch as a carbon source at 30◦C for 48 h.The crude enzyme demonstrated thermophilic properties,exhibiting robust amylase activity across a broad temperature range(30-100◦C),with the highest amylase activity(168.05 U/mg protein)at 65◦C in 50 mM sodium phos-phate buffer pH 6 toward soluble starch.The enzyme efficiently hydrolyzed both raw and gelatinized starches,predominantly yielding maltooligosaccharides(DP3 and DP5)as valuable prebiotics,without producing glucose.The highest sugar yield was obtained from gelatinized potato starch,with 841.58 mg/g substrate(84%con-version),while cassava pulp hydrolysis below the gelatinization temperature achieved a 57.32%conversion,producing 434.65 mg/g maltopentaose as majority equivalent to 75.8%of product mixture.This non-thermal saccharification process without glucose byproduct has significant potential for prebiotic production,adding value to starch-rich agricultural byproducts andcontributing to a sustainable,zero-waste starch industry.
文摘Bacterial cellulose is a versatile material with applications in many industries. However, the widespread uptake of bacterial cellulose faces challenges including high production costs and lack of scalability. One approach to address these obstacles is the use of alternative substrates and media, compared to the Hestrin-Schramm (HS) media. By evaluating and selecting appro- priate media and substrates, the production of bacterial cellulose can be more efficient: enabling sustainable systems and supply chains where less energy and materials are lost, and the output production is increased. The purpose of this paper is to analyze the current landscape of bac- terial cellulose alternative media and substrates (ingredients). Through a systematic review of 198 papers, this review identifies 299 alternative substrates from 12 industries and 101 bacte- rial cellulose-producing strains, which were systematically compared. This review also finds that there are methodological gaps in this field such as data variability, papers mislabelling the HS media or not using a comparison media, and a lack of strain names. This alternative substrate analysis for bacterial cellulose production demonstrates that overall, for some applications al- ternative substrates can be taken into consideration that are not only cheaper, but also produce higher yields than HS media.
