Antibiotic resistance genes(ARGs) are recognized as a primary threat to the sustainability of environment and human health in the 21^(st) century.Nanomaterials(NMs) have attracted substantial attention due to their un...Antibiotic resistance genes(ARGs) are recognized as a primary threat to the sustainability of environment and human health in the 21^(st) century.Nanomaterials(NMs) have attracted substantial attention due to their unique dimensions and structures.Unfortunately,emerging evidence suggests that NMs may facilitate the transmission of ARGs.It is crucial to elucidate how NMs affect the evolution and dissemination of ARGs.The current review comprehensively examines the role of NMs in the widespread transmission of ARGs in aquatic environments and the underlying mechanisms involved in the process.It aims to clarify the effects and mechanisms of NMs on the horizontal gene transfer processes that are associated with ARGs,including the enhancement of cell membrane permeability,the formation of nanopores on membranes,promotion of mutagenesis,and the generation of reactive oxygen species(ROSs).Furthermore,the trade-off between the removal of ARGs and horizontal transfer has been elucidated.The review aspires to guide future research directions,advance knowledge on the implications of NMs in the field of ARGs' transmission,and provide a theoretical foundation for the development of safer and more effective applications of NMs.展开更多
To elucidate the geographical differentiation characteristics and driving mechanisms of Dissolved Organic Matter(DOM)in typical rivers,this study conducted a multi-spectral investigation on three representative river ...To elucidate the geographical differentiation characteristics and driving mechanisms of Dissolved Organic Matter(DOM)in typical rivers,this study conducted a multi-spectral investigation on three representative river types within Shandong Province:The mountainous Dawen River,the plain Tuhai River,and the artificial East Grand Canal.The DOM composition was analyzed using Ultraviolet-Visible(UV-Vis)absorption spectroscopy,Excitation-Emission Matrix(EEM)fluorescence spectroscopy,and parallel factor analysis(PARAFAC),while Principal Component Analysis(PCA)was employed to quantify the synergistic effects of natural processes and anthropogenic activities.Results revealed significant spatial heterogeneity in DOM composition and sources.The plain river exhibited the highest aromaticity(humic-like components:43.3%)due to long-term agricultural non-point source inputs and urban wastewater discharge.The mountain stream,shaped by complex terrain and relatively intact ecosystems,was dominated by autochthonous DOM derived from microbial metabolism,with higher Fluorescence Index(FI=2.12)and biological index(BIX=1.35)than other river types.The artificial canal retained protein-like components(64.2%),largely attributed to winter hydrological stagnation and disturbances from shipping activities.Further analysis demonstrated that geographical settings(e.g.,mountain terrain)and anthropogenic activities(e.g.,agriculture,shipping)jointly regulated DOM composition by altering the balance between input and transformation processes.Integrated fluorescence parameters and PCA results suggested differentiated management strategies:protecting ecological integrity in mountain streams to sustain selfpurification,enhancing non-point source interception in plain rivers,and mitigating shipping pollution in canals.This study systematically reveals the natural-anthropogenic coupling mechanisms driving DOM dynamics in northern China rivers,providing critical insights for precision water environment management at the watershed scale.展开更多
As a large family of RNA helicases,DEAD-box(DDX)RNA helicases play crucial roles in almost all cellular RNA processing activities.However,the role of the DDX gene family in cold tolerance of mei(Prunus mume)remains un...As a large family of RNA helicases,DEAD-box(DDX)RNA helicases play crucial roles in almost all cellular RNA processing activities.However,the role of the DDX gene family in cold tolerance of mei(Prunus mume)remains unclear.In this study,we identified 45 DDX genes through whole-genome analysis unevenly distributed across eight chromosomes and scaffolds of mei.Based on the phylogenetic tree and gene structure analysis,the DDX genes were classified into nine subfamilies based on their motif compositions and intron-exon structures.The results of synteny analysis showed that segmental duplication was considered a major factor contributing to the amplification of the PmDDX family.RNA-Seq and qRT-PCR results revealed differential expression of PmDDX genes under cold stress.Among these,PmDDX39 was significantly up-regulated under cold stress,suggesting its positive role in modulating mei cold tolerance.We found that silenced PmDDX39 under cold stress led to greater damage than the wild seedlings via virus-induced gene silencing(VIGS).Conversely,overexpression of PmDDX39 in Arabidopsis enhanced cold stress tolerance.Moreover,dual luciferase and yeast one-hybrid(Y1H)demonstrated that PmDDX39 directly activates the expression of the C-repeat binding factor(PmCBFf)by binding to its promoters.This study provides new insights into the structure,evolution,and functional role of the PmDDX gene family in mei responses to cold stress.展开更多
Sustainable water,energy and food(WEF)supplies are the bedrock upon which human society depends.Solar-driven interfacial evaporation,combined with electricity generation and cultivation,is a promising approach to miti...Sustainable water,energy and food(WEF)supplies are the bedrock upon which human society depends.Solar-driven interfacial evaporation,combined with electricity generation and cultivation,is a promising approach to mitigate the freshwater,energy and food crises.However,the performance of solar-driven systems decreases significantly during operation due to uncontrollable weather.This study proposes an integrated water/electricity cogeneration-cultivation system with superior thermal management.The energy storage evaporator,consisting of energy storage microcapsules/hydrogel composites,is optimally designed for sustainable desalination,achieving an evaporation rate of around 1.91 kg m^(-2)h^(-1).In the dark,heat released from the phase-change layer supported an evaporation rate of around 0.54kg m^(-2)h^(-1).Reverse electrodialysis harnessed the salinity-gradient energy enhanced during desalination,enabling the long-running WEC system to achieve a power output of~0.3 W m^(-2),which was almost three times higher than that of conventional seawater/surface water mixing.Additionally,an integrated crop irrigation platform utilized system drainage for real-time,on-demand wheat cultivation without secondary contaminants,facilitating seamless WEF integration.This work presents a novel approach to all-day solar water production,electricity generation and crop irrigation,offering a solution and blueprint for the sustainable development of WEF.展开更多
Designing a heterogeneous interface to improve the kinetics of electrocatalysts represents an effective yet challenging approach for enhancing the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).Herei...Designing a heterogeneous interface to improve the kinetics of electrocatalysts represents an effective yet challenging approach for enhancing the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).Herein,a simple MOF-assisted etching-pyrolysis strategy is proposed to fabricate an advanced Mott-Schottky(M–S)electrocatalyst composed of Co/CeO_(2)hetero-nanoparticles embedded within N-doped hollow carbon nanoboxes(H-Co/CeO_(2)@NCBs).Notably,the interfacial Co–O–Ce bond bridging productively facilitates the electron transfer and modulates the charge distribution of the active center,thereby contributing to the ORR/OER kinetics.As expected,the optimal M–S H-Co/CeO_(2)@NCBs catalyst exhibits promising bifunctional electrocatalytic activity with a small potential discrepancy of 0.65 V.Theoretical calculations reveal that the built-in electric field in the M–S heterojunction promotes electron transfer in oxygen electrocatalysis and the interfacial bridge-induced electron redistribution optimizes the adsorption/desorption of the oxygen intermediates,leading to reduced activation energy for the bifunctional ORR/OER reactions.Importantly,H-Co/CeO_(2)@NCBs-assembled Zn-air battery(ZAB)delivers high power density(179.8 mW cm^(−2))and long-term stability(400 h).Furthermore,the assembled flexible solid-state ZAB with H-Co/CeO_(2)@NCBs cathode also exhibits excellent charge–discharge reversibility and flexibility at various bending angles.This work provides a novel perspective on developing efficient and stable M–S bifunctional oxygen electrocatalysts.展开更多
Soil organic carbon(SOC)dynamics significantly influence ecosystem carbon source-sink balance,particularly in agroecosystems.However,uncertainty remains regarding optimal land use types for maximizing farmland carbon ...Soil organic carbon(SOC)dynamics significantly influence ecosystem carbon source-sink balance,particularly in agroecosystems.However,uncertainty remains regarding optimal land use types for maximizing farmland carbon storage across different soil types,and identifying effective land management practices for enhanced carbon accumulation is essential for reducing agricultural emissions and strengthening carbon sinks.This study examined SOC variations in eastern Yunnan’s subtropical highlands(2,132 sites),analyzing topsoil(0–20 cm)across five land uses(dryland,irrigated land,forestland,grassland and plantation)of five soil types(red,yellow,yellowbrown,brown,purple).The investigation explored relationships between SOC and edaphic factors(26 elements)to determine SOC influencing factors.The study area demonstrated a mean SOC content of 27.78 g kg^(–1),with distinct spatial heterogeneity characterized by lower values in the southwestern sector and higher concentrations in the northeastern region.Brown soils displayed the highest SOC content(P<0.05),followed by yellow-brown then red,yellow,and purple soils.Irrigation significantly enhanced SOC storage,particularly in brown soils where irrigated land contained 2.2-,2.4-,and 1.6-times higher SOC than forestland,grassland,and dryland,respectively.Similar irrigation benefits occurred in purple,yellow,and yellow-brown soils,indicating moisture limitation as the primary SOC constraint.Notably,SOC exhibited strong positive correlations with nitrogen,sulfur,and selenium.Nitrogen fertilization demonstrated dual benefits:enhancing SOC sequestration and promoting Se enrichment in crops,potentially supporting specialty agriculture.Although land use impacts on SOC varied across soil types(P>0.05),irrigation consistently emerged as the optimal management for carbon sink enhancement.These findings suggest that targeted water management could effectively reduce farmland carbon emissions in moisture-limited subtropical highlands.Strategic nitrogen application offers co-benefits for soil fertility and selenium biofortification,providing practical pathways for climate-smart agriculture in similar ecoregions.展开更多
基金supported by the State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology) (No.2022TS13)the key projects of National Natural Science Foundation of China (No.2019YFC0408503)the Key Research Program of Wuhan (No.2022022202015015)。
文摘Antibiotic resistance genes(ARGs) are recognized as a primary threat to the sustainability of environment and human health in the 21^(st) century.Nanomaterials(NMs) have attracted substantial attention due to their unique dimensions and structures.Unfortunately,emerging evidence suggests that NMs may facilitate the transmission of ARGs.It is crucial to elucidate how NMs affect the evolution and dissemination of ARGs.The current review comprehensively examines the role of NMs in the widespread transmission of ARGs in aquatic environments and the underlying mechanisms involved in the process.It aims to clarify the effects and mechanisms of NMs on the horizontal gene transfer processes that are associated with ARGs,including the enhancement of cell membrane permeability,the formation of nanopores on membranes,promotion of mutagenesis,and the generation of reactive oxygen species(ROSs).Furthermore,the trade-off between the removal of ARGs and horizontal transfer has been elucidated.The review aspires to guide future research directions,advance knowledge on the implications of NMs in the field of ARGs' transmission,and provide a theoretical foundation for the development of safer and more effective applications of NMs.
基金supported by the National Natural Science Foundation(42472325)the Fundamental Research Funds of Chinese Academy of Geological Science(SK202103).
文摘To elucidate the geographical differentiation characteristics and driving mechanisms of Dissolved Organic Matter(DOM)in typical rivers,this study conducted a multi-spectral investigation on three representative river types within Shandong Province:The mountainous Dawen River,the plain Tuhai River,and the artificial East Grand Canal.The DOM composition was analyzed using Ultraviolet-Visible(UV-Vis)absorption spectroscopy,Excitation-Emission Matrix(EEM)fluorescence spectroscopy,and parallel factor analysis(PARAFAC),while Principal Component Analysis(PCA)was employed to quantify the synergistic effects of natural processes and anthropogenic activities.Results revealed significant spatial heterogeneity in DOM composition and sources.The plain river exhibited the highest aromaticity(humic-like components:43.3%)due to long-term agricultural non-point source inputs and urban wastewater discharge.The mountain stream,shaped by complex terrain and relatively intact ecosystems,was dominated by autochthonous DOM derived from microbial metabolism,with higher Fluorescence Index(FI=2.12)and biological index(BIX=1.35)than other river types.The artificial canal retained protein-like components(64.2%),largely attributed to winter hydrological stagnation and disturbances from shipping activities.Further analysis demonstrated that geographical settings(e.g.,mountain terrain)and anthropogenic activities(e.g.,agriculture,shipping)jointly regulated DOM composition by altering the balance between input and transformation processes.Integrated fluorescence parameters and PCA results suggested differentiated management strategies:protecting ecological integrity in mountain streams to sustain selfpurification,enhancing non-point source interception in plain rivers,and mitigating shipping pollution in canals.This study systematically reveals the natural-anthropogenic coupling mechanisms driving DOM dynamics in northern China rivers,providing critical insights for precision water environment management at the watershed scale.
基金supported by the Fundamental Research Funds for the Central Universities(Grant No.QNTD202503)Forestry and Grassland Science and Technology Innovation Youth Top Talent Project of China(Grant No.2020132608)Beijing High-Precision Discipline Project,Discipline of Ecological Environment of Urban and Rural Human Settlements.
文摘As a large family of RNA helicases,DEAD-box(DDX)RNA helicases play crucial roles in almost all cellular RNA processing activities.However,the role of the DDX gene family in cold tolerance of mei(Prunus mume)remains unclear.In this study,we identified 45 DDX genes through whole-genome analysis unevenly distributed across eight chromosomes and scaffolds of mei.Based on the phylogenetic tree and gene structure analysis,the DDX genes were classified into nine subfamilies based on their motif compositions and intron-exon structures.The results of synteny analysis showed that segmental duplication was considered a major factor contributing to the amplification of the PmDDX family.RNA-Seq and qRT-PCR results revealed differential expression of PmDDX genes under cold stress.Among these,PmDDX39 was significantly up-regulated under cold stress,suggesting its positive role in modulating mei cold tolerance.We found that silenced PmDDX39 under cold stress led to greater damage than the wild seedlings via virus-induced gene silencing(VIGS).Conversely,overexpression of PmDDX39 in Arabidopsis enhanced cold stress tolerance.Moreover,dual luciferase and yeast one-hybrid(Y1H)demonstrated that PmDDX39 directly activates the expression of the C-repeat binding factor(PmCBFf)by binding to its promoters.This study provides new insights into the structure,evolution,and functional role of the PmDDX gene family in mei responses to cold stress.
基金supported by the National Natural Science Foundation of China(No.52070057)China Postdoctoral Science Foundation(No.2023M730855)Heilongjiang Postdoctoral Fund(No.LBH-Z22183)for financial support。
文摘Sustainable water,energy and food(WEF)supplies are the bedrock upon which human society depends.Solar-driven interfacial evaporation,combined with electricity generation and cultivation,is a promising approach to mitigate the freshwater,energy and food crises.However,the performance of solar-driven systems decreases significantly during operation due to uncontrollable weather.This study proposes an integrated water/electricity cogeneration-cultivation system with superior thermal management.The energy storage evaporator,consisting of energy storage microcapsules/hydrogel composites,is optimally designed for sustainable desalination,achieving an evaporation rate of around 1.91 kg m^(-2)h^(-1).In the dark,heat released from the phase-change layer supported an evaporation rate of around 0.54kg m^(-2)h^(-1).Reverse electrodialysis harnessed the salinity-gradient energy enhanced during desalination,enabling the long-running WEC system to achieve a power output of~0.3 W m^(-2),which was almost three times higher than that of conventional seawater/surface water mixing.Additionally,an integrated crop irrigation platform utilized system drainage for real-time,on-demand wheat cultivation without secondary contaminants,facilitating seamless WEF integration.This work presents a novel approach to all-day solar water production,electricity generation and crop irrigation,offering a solution and blueprint for the sustainable development of WEF.
基金supported by the National Natural Science Foundation of China(U24A20550,52273264 and 52470073)the Key Project of the Heilongjiang Provincial Natural Science Foundation(ZD2024B001)Outstanding Youth Fund of Heilongjiang Province(JQ2022E005).
文摘Designing a heterogeneous interface to improve the kinetics of electrocatalysts represents an effective yet challenging approach for enhancing the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).Herein,a simple MOF-assisted etching-pyrolysis strategy is proposed to fabricate an advanced Mott-Schottky(M–S)electrocatalyst composed of Co/CeO_(2)hetero-nanoparticles embedded within N-doped hollow carbon nanoboxes(H-Co/CeO_(2)@NCBs).Notably,the interfacial Co–O–Ce bond bridging productively facilitates the electron transfer and modulates the charge distribution of the active center,thereby contributing to the ORR/OER kinetics.As expected,the optimal M–S H-Co/CeO_(2)@NCBs catalyst exhibits promising bifunctional electrocatalytic activity with a small potential discrepancy of 0.65 V.Theoretical calculations reveal that the built-in electric field in the M–S heterojunction promotes electron transfer in oxygen electrocatalysis and the interfacial bridge-induced electron redistribution optimizes the adsorption/desorption of the oxygen intermediates,leading to reduced activation energy for the bifunctional ORR/OER reactions.Importantly,H-Co/CeO_(2)@NCBs-assembled Zn-air battery(ZAB)delivers high power density(179.8 mW cm^(−2))and long-term stability(400 h).Furthermore,the assembled flexible solid-state ZAB with H-Co/CeO_(2)@NCBs cathode also exhibits excellent charge–discharge reversibility and flexibility at various bending angles.This work provides a novel perspective on developing efficient and stable M–S bifunctional oxygen electrocatalysts.
基金funded by the Yunnan Provincial Key Programs for Basic Research Project,China(202301AS070087)the Yunnan Provincial R&D Program,China(202405AF140014 and 202302AO370015)the National Natural Science Foundation of China(42307058).
文摘Soil organic carbon(SOC)dynamics significantly influence ecosystem carbon source-sink balance,particularly in agroecosystems.However,uncertainty remains regarding optimal land use types for maximizing farmland carbon storage across different soil types,and identifying effective land management practices for enhanced carbon accumulation is essential for reducing agricultural emissions and strengthening carbon sinks.This study examined SOC variations in eastern Yunnan’s subtropical highlands(2,132 sites),analyzing topsoil(0–20 cm)across five land uses(dryland,irrigated land,forestland,grassland and plantation)of five soil types(red,yellow,yellowbrown,brown,purple).The investigation explored relationships between SOC and edaphic factors(26 elements)to determine SOC influencing factors.The study area demonstrated a mean SOC content of 27.78 g kg^(–1),with distinct spatial heterogeneity characterized by lower values in the southwestern sector and higher concentrations in the northeastern region.Brown soils displayed the highest SOC content(P<0.05),followed by yellow-brown then red,yellow,and purple soils.Irrigation significantly enhanced SOC storage,particularly in brown soils where irrigated land contained 2.2-,2.4-,and 1.6-times higher SOC than forestland,grassland,and dryland,respectively.Similar irrigation benefits occurred in purple,yellow,and yellow-brown soils,indicating moisture limitation as the primary SOC constraint.Notably,SOC exhibited strong positive correlations with nitrogen,sulfur,and selenium.Nitrogen fertilization demonstrated dual benefits:enhancing SOC sequestration and promoting Se enrichment in crops,potentially supporting specialty agriculture.Although land use impacts on SOC varied across soil types(P>0.05),irrigation consistently emerged as the optimal management for carbon sink enhancement.These findings suggest that targeted water management could effectively reduce farmland carbon emissions in moisture-limited subtropical highlands.Strategic nitrogen application offers co-benefits for soil fertility and selenium biofortification,providing practical pathways for climate-smart agriculture in similar ecoregions.
