The sustainable development of aquaculture industry is deeply constrained by pathogens and diseases,and traditional detection methods are difficult to adapt to the needs of intensive aquaculture due to low efficiency ...The sustainable development of aquaculture industry is deeply constrained by pathogens and diseases,and traditional detection methods are difficult to adapt to the needs of intensive aquaculture due to low efficiency and insufficient sensitivity.This article reviews the progress of rapid detection technology for aquatic pathogens based on molecular biology,immunology,biosensors,etc.,and analyzes the application value of innovative methods such as isothermal amplification and CRISPR.This technology injects core momentum into the new quality productivity of fisheries through early and precise identification of pathogens:reducing aquaculture losses to improve resource efficiency,promoting the transformation of aquaculture models to data-driven,ensuring the safety of aquatic products to enhance the competitiveness of the industry chain.The current technology has shortcomings such as lagging standardization,weak on-site anti-interference ability,and insufficient recognition of new pathogens.In the future,we need to focus on technological integration and innovation,intelligent upgrading,and standardization construction,promote technology from laboratories to industrial applications,and provide continuous support for the high-quality development of fisheries.展开更多
Two important mycotoxins, aflatoxin and fumonisin, are among the most potent naturally occurring carcinogens, contaminating maize(Zea mays) and affecting crop yield and quality.Resistance of maize to pre-harvest mycot...Two important mycotoxins, aflatoxin and fumonisin, are among the most potent naturally occurring carcinogens, contaminating maize(Zea mays) and affecting crop yield and quality.Resistance of maize to pre-harvest mycotoxin contamination, specifically aflatoxin produced by Aspergillus flavus and fumonisin produced by Fusarium verticillioides, is a goal in breeding programs that screen for these important traits with the aim of developing resistant commercial hybrids. We conducted two years of field evaluations on 87 inbred lines originating primarily in China and Mexico and not previously screened for resistance.The objectives of our study were to identify resistant germplasm for breeding purposes and to examine possible relationships between resistances to the two mycotoxins. Aflatoxin and fumonisin were present in samples harvested from all lines in both years.Concentrations of total aflatoxin ranged from 52.00 ± 20.00 to 1524.00 ± 396.00 μg kg^(-1),while those of fumonisin ranged from 0.60 ± 0.06 to 124.00 ± 19.50 mg kg^(-1). The inbred lines TUN15, TUN61, TUN37, CY2, and TUN49 showed the lowest aflatoxin accumulation and CN1, GT601, TUN09, TUN61, and MP717 the lowest fumonisin accumulation. TUN61 showed the lowest accumulation of both mycotoxins. This study confirmed previous observations that high levels of aflatoxin can coexist with fumonisin, with 55 maize lines showing a positive correlation coefficient between the concentrations of aflatoxin and fumonisin and 32 lines showing a negative correlation coefficient. These selected lines,particularly TUN61, may provide sources of resistance to mycotoxin contamination in breeding programs. However, the mechanism of resistance in this germplasm remains to be identified. Future research should also address factors that influence the fungus–plant interaction, such as herbivory and environmental stress.展开更多
基金supported by the National Modern Agricultural Industry Technology System(CARS-45-33)Innovation Team of Tianjin Freshwater Aquaculture Industry Technology System(ITTFRS2021000-002,ITTFRS2021000-001)+1 种基金Tianjin Science and Technology Plan Project(24KPHDRC00280,24ZYCGSN00250,23YDTPJC00420)the Open Fund Project of Key Laboratory of Ocean Observation Technology,MNR(No.2023klootA03).
文摘The sustainable development of aquaculture industry is deeply constrained by pathogens and diseases,and traditional detection methods are difficult to adapt to the needs of intensive aquaculture due to low efficiency and insufficient sensitivity.This article reviews the progress of rapid detection technology for aquatic pathogens based on molecular biology,immunology,biosensors,etc.,and analyzes the application value of innovative methods such as isothermal amplification and CRISPR.This technology injects core momentum into the new quality productivity of fisheries through early and precise identification of pathogens:reducing aquaculture losses to improve resource efficiency,promoting the transformation of aquaculture models to data-driven,ensuring the safety of aquatic products to enhance the competitiveness of the industry chain.The current technology has shortcomings such as lagging standardization,weak on-site anti-interference ability,and insufficient recognition of new pathogens.In the future,we need to focus on technological integration and innovation,intelligent upgrading,and standardization construction,promote technology from laboratories to industrial applications,and provide continuous support for the high-quality development of fisheries.
基金partially supported by the U.S.Department of Agriculture-Agricultural Research Service(USDA-ARS)the Georgia Agricultural Commodity Commission for Corn+1 种基金the National Corn Growers AssociationAMCOE(Aflatoxin Mitigation Center of Excellence)
文摘Two important mycotoxins, aflatoxin and fumonisin, are among the most potent naturally occurring carcinogens, contaminating maize(Zea mays) and affecting crop yield and quality.Resistance of maize to pre-harvest mycotoxin contamination, specifically aflatoxin produced by Aspergillus flavus and fumonisin produced by Fusarium verticillioides, is a goal in breeding programs that screen for these important traits with the aim of developing resistant commercial hybrids. We conducted two years of field evaluations on 87 inbred lines originating primarily in China and Mexico and not previously screened for resistance.The objectives of our study were to identify resistant germplasm for breeding purposes and to examine possible relationships between resistances to the two mycotoxins. Aflatoxin and fumonisin were present in samples harvested from all lines in both years.Concentrations of total aflatoxin ranged from 52.00 ± 20.00 to 1524.00 ± 396.00 μg kg^(-1),while those of fumonisin ranged from 0.60 ± 0.06 to 124.00 ± 19.50 mg kg^(-1). The inbred lines TUN15, TUN61, TUN37, CY2, and TUN49 showed the lowest aflatoxin accumulation and CN1, GT601, TUN09, TUN61, and MP717 the lowest fumonisin accumulation. TUN61 showed the lowest accumulation of both mycotoxins. This study confirmed previous observations that high levels of aflatoxin can coexist with fumonisin, with 55 maize lines showing a positive correlation coefficient between the concentrations of aflatoxin and fumonisin and 32 lines showing a negative correlation coefficient. These selected lines,particularly TUN61, may provide sources of resistance to mycotoxin contamination in breeding programs. However, the mechanism of resistance in this germplasm remains to be identified. Future research should also address factors that influence the fungus–plant interaction, such as herbivory and environmental stress.
