Histochemical localization of uterine E2 receptors using fluorescence conjugated E2 has more advantages than the autoradiographic method in its simplicity, rapidity and freedom from isotope contamination.The 4-amino-N...Histochemical localization of uterine E2 receptors using fluorescence conjugated E2 has more advantages than the autoradiographic method in its simplicity, rapidity and freedom from isotope contamination.The 4-amino-N-fluorescein-isothicoyanate-17βestradiol （4NHEF） was synthesized and its binding capacity to the nterine E2 receptors was also identified in the rat.展开更多
Just as Tang and Jiang pointed out in ref. [1], the total π-electron energy E<sub>π</sub> of a conjugate molecule is of significance for understanding the stability and aromatic character of molecule or ...Just as Tang and Jiang pointed out in ref. [1], the total π-electron energy E<sub>π</sub> of a conjugate molecule is of significance for understanding the stability and aromatic character of molecule or the reaction route. They also proposed the expanded formula for evaluating E<sub>π</sub> by using molecular moment μ<sub>1</sub>.展开更多
The introduction of a 15-methyl group into a prostaglandin E2 （or its methyl ester） molecule blocks the metabolic action of the 15-hydroxyprostaglandin dehydrogenase and results in a considerable increase of the bio...The introduction of a 15-methyl group into a prostaglandin E2 （or its methyl ester） molecule blocks the metabolic action of the 15-hydroxyprostaglandin dehydrogenase and results in a considerable increase of the biological activities as well as prolongs the duration of the action of resulting derivative. Up to date, only one route for展开更多
Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and...Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - density lipoprotein cholesterol ( HDL - C) ; HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely; HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group, CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、 LDL-C、apoA1、apoB、CD8 + 、MDA of men all presented a positive correlation with E_2/T respectively, their correlation coefficients (γ) were 0. 262、0. 136、 0. 532、0. 379、0. 394、0. 234 (P < 0. 001 ) ; HDL - C、 HDL- C/TC、HDL- C/LDL- C、CD3 + 、CD4 +/CD8 + 、SOD showed a negatively correlation with E_2/T respectively, γequaled - 0.563、- 0.332、- 0.654、- 0.1530、-0.4140、-0.236(P<0.001). In women, the serum concentrations of FSH、LH increased significantly after menopause; PRL increased little with aging; compared with young group, E_2 and P in postmenopausal groups reduced obviously, E_2/P revealed significant reduce with aging. T enhanced significantly after menopause, but nor did FT. E_2, P and the ratio of E_2/P were negatively correlated with age respectively by bivariate correlation analysis, and a positive relation between T and age. After 70 years old, the level of TC increased obviously, and so did that of TG after menopause; HDL decreased with aging, but LDL increased after 70, with the result that the ratios of HDL- C/TC and HDL- C/LDL- C all reduced with aging; apoA1 decreased gently after 70, but apoB increased signifi- cantly after menopause; correspondingly, the ratio of apoA1/apoB declined obviously. The concentration of GLU increased with aging. CD3 + and CD4 + didn't change until 60, but reduced after 60. Compared with the young groups, CD8 + remained unchanged, CD4 +/CD8 + reduced greatly with aging, CD4 + and CD8 + presented a negatively correlation with age respectively. The value of MDA in serum of women increased notably after 70 years old, but SOD activity already decreased significantly from 60. By partial correlation analysis (controlling BMI, FSH, LH and PRL), HDL-C、CD4 +、CD4 +/CD8 + showed a certain correlation with E_2/P respectively; γ were 0. 245、 0.157、0. 154 ( P <0.05 ) ; TG、 LDL、 apoB、 apoA1/ apoB、SOD presented a negatively correlation with E_2/P respectively, γ were 0. 452、 0. 236、 0.321、 0. 135、 0.156、0.154、0.426 ( P < 0.05 ). Conclusions The Disequilibrium of SH had correlations with lipid profile, cellular immunological function and lipid peroxides and oxidation system; these suggested SH took an important role in the process of aging.展开更多
Ⅰ. INTRODUCTION In 1974, Collier et al. reported that morphine and its congeners inhibited the activity of adenylate cyclase （AC） of rat brain homogenates, stimulated by prostaglandins E1 （PGE1） and E, （PGE2）, ...Ⅰ. INTRODUCTION In 1974, Collier et al. reported that morphine and its congeners inhibited the activity of adenylate cyclase （AC） of rat brain homogenates, stimulated by prostaglandins E1 （PGE1） and E, （PGE2）, and that the inhibition was mediated by opiate receptors. Sharma et al. also demonstrated that morphine and a series of opiates could inhibit this enzyme in neuroblastoma×glioma hybrid cells stimulated by PGE1,展开更多
基金This work was supported by the World Health Organization
文摘Histochemical localization of uterine E2 receptors using fluorescence conjugated E2 has more advantages than the autoradiographic method in its simplicity, rapidity and freedom from isotope contamination.The 4-amino-N-fluorescein-isothicoyanate-17βestradiol （4NHEF） was synthesized and its binding capacity to the nterine E2 receptors was also identified in the rat.
文摘Just as Tang and Jiang pointed out in ref. [1], the total π-electron energy E<sub>π</sub> of a conjugate molecule is of significance for understanding the stability and aromatic character of molecule or the reaction route. They also proposed the expanded formula for evaluating E<sub>π</sub> by using molecular moment μ<sub>1</sub>.
文摘The introduction of a 15-methyl group into a prostaglandin E2 （or its methyl ester） molecule blocks the metabolic action of the 15-hydroxyprostaglandin dehydrogenase and results in a considerable increase of the biological activities as well as prolongs the duration of the action of resulting derivative. Up to date, only one route for
文摘Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - density lipoprotein cholesterol ( HDL - C) ; HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely; HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group, CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、 LDL-C、apoA1、apoB、CD8 + 、MDA of men all presented a positive correlation with E_2/T respectively, their correlation coefficients (γ) were 0. 262、0. 136、 0. 532、0. 379、0. 394、0. 234 (P < 0. 001 ) ; HDL - C、 HDL- C/TC、HDL- C/LDL- C、CD3 + 、CD4 +/CD8 + 、SOD showed a negatively correlation with E_2/T respectively, γequaled - 0.563、- 0.332、- 0.654、- 0.1530、-0.4140、-0.236(P<0.001). In women, the serum concentrations of FSH、LH increased significantly after menopause; PRL increased little with aging; compared with young group, E_2 and P in postmenopausal groups reduced obviously, E_2/P revealed significant reduce with aging. T enhanced significantly after menopause, but nor did FT. E_2, P and the ratio of E_2/P were negatively correlated with age respectively by bivariate correlation analysis, and a positive relation between T and age. After 70 years old, the level of TC increased obviously, and so did that of TG after menopause; HDL decreased with aging, but LDL increased after 70, with the result that the ratios of HDL- C/TC and HDL- C/LDL- C all reduced with aging; apoA1 decreased gently after 70, but apoB increased signifi- cantly after menopause; correspondingly, the ratio of apoA1/apoB declined obviously. The concentration of GLU increased with aging. CD3 + and CD4 + didn't change until 60, but reduced after 60. Compared with the young groups, CD8 + remained unchanged, CD4 +/CD8 + reduced greatly with aging, CD4 + and CD8 + presented a negatively correlation with age respectively. The value of MDA in serum of women increased notably after 70 years old, but SOD activity already decreased significantly from 60. By partial correlation analysis (controlling BMI, FSH, LH and PRL), HDL-C、CD4 +、CD4 +/CD8 + showed a certain correlation with E_2/P respectively; γ were 0. 245、 0.157、0. 154 ( P <0.05 ) ; TG、 LDL、 apoB、 apoA1/ apoB、SOD presented a negatively correlation with E_2/P respectively, γ were 0. 452、 0. 236、 0.321、 0. 135、 0.156、0.154、0.426 ( P < 0.05 ). Conclusions The Disequilibrium of SH had correlations with lipid profile, cellular immunological function and lipid peroxides and oxidation system; these suggested SH took an important role in the process of aging.
文摘Ⅰ. INTRODUCTION In 1974, Collier et al. reported that morphine and its congeners inhibited the activity of adenylate cyclase （AC） of rat brain homogenates, stimulated by prostaglandins E1 （PGE1） and E, （PGE2）, and that the inhibition was mediated by opiate receptors. Sharma et al. also demonstrated that morphine and a series of opiates could inhibit this enzyme in neuroblastoma×glioma hybrid cells stimulated by PGE1,
