Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hep...Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hepatic ApoE expression(ΔApoE),24-month-oldΔApoE mice displayed a 95%reduction in circulating ApoE levels and significantly improved fracture healing.ApoE treatment of aged BMSCs inhibited osteoblast differentiation in tissue culture models;RNA-seq,Western blot,immunofluorescence,and RT-PCR analyses indicated that the Wnt/β-catenin pathway is the target of this inhibition.Indeed,we showed that ApoE had no effect on cultures with stabilizedβ-catenin levels.Next,we determined that Lrp4 serves as the osteoblast cell surface receptor to ApoE,as expression of Lrp4 is required in ApoE-based inhibition of Wnt/β-catenin signaling and osteoblast differentiation.Importantly,we validated this ApoE-Lrp4-Wnt/β-catenin molecular mechanism in human osteoblast differentiation.Finally,we identified an ApoE-neutralizing antibody(NAb)and used it to treat aged,wildtype mice 3 days after fracture surgery resulting in fracture calluses with 35%more bone deposition.Our work here identifies novel liver-to-bone cross-talk and a noninvasive,translatable therapeutic intervention for aged bone regeneration.展开更多
Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh...Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh)regulates NSC quiescence and differentiation within neurogenic niches,glioblastoma cells exploit th ese pathways to enhance their adaptability and invasiveness.The involvement of muscarinic(M3)and nicotinic(α7)receptors in both cell types suggests that glioblastoma retains neural progenitor-like traits,contributing to its plasticity and resilience.This article explores the shared cholinergic mechanisms between NSCs and GSCs,highlighting their role in both neural development and glioblastoma progression.展开更多
British Prime Minister Keir Starmer’s visit to China underscores economic cooperation and strategic stability amid global uncertainties.U.K.Prime Minister Keir Starmer’s visit to China in January marks a significant...British Prime Minister Keir Starmer’s visit to China underscores economic cooperation and strategic stability amid global uncertainties.U.K.Prime Minister Keir Starmer’s visit to China in January marks a significant shift of Britain toward a more pragmatic and stability-oriented approach in Sino-British relations.After years of political tensions and limited high-level engagement,this trip underscores London’s intent to rebuild ties based on mutual economic and strategic interests.展开更多
Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remai...Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remains largely unknown.Lateral organ boundary(LOB)domain(LBD)proteins are pivotal regulators of plant growth and development.To date,certain LOB/LBD transcription factors are seemingly implicated in fruit softening.In this study,we identified 42 LOB/LBD genes in the peach genome.Expression analysis showed a significant upregulation of PpLOB1 transcripts toward peach fruit ripening.PpLOB1 was classified into Class II subgroup,and showed high sequence similarity to several softening-related LOB/LBD transcription factors.Transient transformation assays showed that PpLOB1 positively modulates peach softening.Further experiments demonstrated that PpLOB1 directly targets and activates the promoters of pectate lyase 1(PpPL1)and PpPL15,thereby contributing to the regulation of fruit softening.Additionally,PpNAP4 up-regulates PpLOB1 expression by binding to its promoter.Meanwhile,our findings revealed that PpNAP4 and PpNAP6 cooperatively modulate the expression of Pp LOB1.Taken together,our findings revealed a novel regulatory module involving PpNAP4 and PpLOB1 that modulates peach fruit softening.展开更多
The application of deep learning in fabric defect detection has become increasingly widespread.To address false positives and false negatives in fabric roll seam detection,and to improve automation efficiency and prod...The application of deep learning in fabric defect detection has become increasingly widespread.To address false positives and false negatives in fabric roll seam detection,and to improve automation efficiency and product quality,we propose the Multi-scale Context DeepLabV3+(MSC-DeepLabV3+),a semantic segmentation network designed for fabric roll seam detection,based on DeepLabV3+.The model improvements include enhancing the backbone performance through optimization of the UIB-MobileNetV2 network;designing the Dynamic Atrous and Sliding-window Fusion(DASF)module to improve adaptability to multi-scale seam structures with dynamic dilation rates and a sliding-window mechanism;and utilizing the Progressive Low-level Feature Fusion(PLFF)module to progressively restore seam boundary details via shallow feature fusion.Additionally,an enhanced 3-SE attention mechanism is employed,replacing the direct concatenation operation.Experimental results show thatMSCDeepLabV3+outperforms classical and recent segmentation models.Compared to DeepLabV3+with an Xception backbone,MSC-DeepLabV3+achieves a mean intersection over union(mIoU)of 92.30%and the boundary Fscore(BF)of 92.54%,representing improvements of 3.04%and 3.14%,respectively.Moreover,the model complexity is significantly reduced,with the model parameters(params)decreasing to 3.44M and Frames Per Second(FPS)increasing from 101 to 273,demonstrating its potential for deployment in resource-constrained industrial scenarios.展开更多
This study explores the nonlinear resonance of a rotating solar sail membrane exposed to time-varying solar thermal and solar radiation pressure.The sail membrane is modeled using a cantilever membrane,applying the vo...This study explores the nonlinear resonance of a rotating solar sail membrane exposed to time-varying solar thermal and solar radiation pressure.The sail membrane is modeled using a cantilever membrane,applying the von Kármán theory for membrane large deflection.The membrane’s nonlinear equation is derived by employing the Lagrange equation while accounting for excitations from solar thermal and radiation pressure.The equation is solved via the Rayleigh-Ritz method.The bifurcation diagram of membrane motion is applied to reveal membrane resonance responses under different solar sail rotating frequencies.The displacement time history,phase portrait,Poincarémap,frequency spectrum,and the largest Lyapunov exponent are used to study nonlinear vibrations that occur near resonance regions.The results indicate that time-varying thermal loading excites membrane motions with multiple natural frequencies by the parametric resonance mechanics,leading to the onset of membrane chaotic motion.The membrane’s primary resonance is stimulated in harmonic oscillation by the time-varying radiation pressure.The divergence instability caused by thermal excitation is also illustrated by comparing the membrane’s vibration amplitude with and without thermal excitation.The membrane’s nonlinear vibration characteristics vary significantly with solar illumination angles,the membrane’s thermal expansion coefficients,and structural damping.展开更多
The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside thei...The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments.One such species,B.calcarea,has been described from the Chandalaz Mountains in northeastern Asia(Russia).This study aimed to investigate the genome structure and evolutionary history of B.calcarea.However,our analyses reveal that the species does not belong to Boechera.Instead,an integrative approach combining cytogenetic,phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae.The diploid Parryodes calcarea(2n=16)exhibits Arabideae-specific chromosomal signatures,including multiple centromere repositionings.These findings clarify the misclassification of P.calcarea as Boechera,leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World.This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe,the Arabideae.展开更多
Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inh...Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation of these new neurons in the olfactory bulb supports both structural and functional plasticity, aiding in circuit remodeling triggered by memory and learning processes. However, the presence of these neurons, coupled with the cellular diversity within the olfactory bulb, presents an ongoing challenge in understanding its network organization and function. Moreover,the continuous integration of new neurons in the olfactory bulb plays a pivotal role in regulating olfactory information processing. This adaptive process responds to changes in epithelial composition and contributes to the formation of olfactory memories by modulating cellular connectivity within the olfactory bulb and interacting intricately with higher-order brain regions. The role of adult neurogenesis in olfactory bulb functions remains a topic of debate. Nevertheless, the functionality of the olfactory bulb is intricately linked to the organization of granule cells around mitral and tufted cells. This organizational pattern significantly impacts output, network behavior, and synaptic plasticity, which are crucial for olfactory perception and memory. Additionally, this organization is further shaped by axon terminals originating from cortical and subcortical regions. Despite the crucial role of olfactory bulb in brain functions and behaviors related to olfaction, these complex and highly interconnected processes have not been comprehensively studied as a whole. Therefore, this manuscript aims to discuss our current understanding and explore how neural plasticity and olfactory neurogenesis contribute to enhancing the adaptability of the olfactory system. These mechanisms are thought to support olfactory learning and memory, potentially through increased complexity and restructuring of neural network structures, as well as the addition of new granule granule cells that aid in olfactory adaptation. Additionally, the manuscript underscores the importance of employing precise methodologies to elucidate the specific roles of adult neurogenesis amidst conflicting data and varying experimental paradigms. Understanding these processes is essential for gaining insights into the complexities of olfactory function and behavior.展开更多
BACKGROUND Coronavirus disease 2019(COVID-19)disrupted healthcare and led to increased telehealth use.We explored the impact of COVID-19 on liver transplant evaluation(LTE).AIM To understand the impact of telehealth o...BACKGROUND Coronavirus disease 2019(COVID-19)disrupted healthcare and led to increased telehealth use.We explored the impact of COVID-19 on liver transplant evaluation(LTE).AIM To understand the impact of telehealth on LTE during COVID-19 and to identify disparities in outcomes disaggregated by sociodemographic factors.METHODS This was a retrospective study of patients who initiated LTE at our center from 3/16/20-3/16/21(“COVID-19 era”)and the year prior(3/16/19-3/15/20,“pre-COVID-19 era”).We compared LTE duration times between eras and explored the effects of telehealth and inpatient evaluations on LTE duration,listing,and pretransplant mortality.RESULTS One hundred and seventy-eight patients were included in the pre-COVID-19 era cohort and one hundred and ninety-nine in the COVID-19 era cohort.Twentynine percent(58/199)of COVID-19 era initial LTE were telehealth,compared to 0%(0/178)pre-COVID-19.There were more inpatient evaluations during COVID-19 era(40%vs 28%,P<0.01).Among outpatient encounters,telehealth use for initial LTE during COVID-19 era did not impact likelihood of listing,pretransplant mortality,or time to LTE and listing.Median times to LTE and listing during COVID-19 were shorter than pre-COVID-19,driven by increased inpatient evaluations.Sociodemographic factors were not predictive of telehealth.CONCLUSION COVID-19 demonstrates a shift to telehealth and inpatient LTE.Telehealth does not impact LTE or listing duration,likelihood of listing,or mortality,suggesting telehealth may facilitate LTE without negative outcomes.展开更多
Appreciation of soft-tissue thickness(STT)at surgical sites is an increasingly recognized aspect of arthroplasty procedures as it may potentially impacting postoperative outcomes.Recent research has focused on the pre...Appreciation of soft-tissue thickness(STT)at surgical sites is an increasingly recognized aspect of arthroplasty procedures as it may potentially impacting postoperative outcomes.Recent research has focused on the predictive value of preoperative STT measurements for complications following various forms of arthroplasty,particularly infections,across procedures such as total knee,hip,shoulder,and ankle replacements.Several studies have indicated that increased STT is associated with a higher risk of complications,including infection and wound healing issues.The assessment of STT before surgery could play a crucial role in identifying patients at a higher risk of complications and may be instru-mental in guiding preoperative planning to optimize outcomes in arthroplasty procedures.Standardized measurement techniques and further research are essential to enhance the reliability and clinical utility of STT assessment for arthro-plasty surgery.展开更多
The U.S.imposition of high tariffs on Chinese goods has triggered short-term strains on China’s exports.At the same time,it has also accelerated its strategic pivot toward technological self-reliance,regional integra...The U.S.imposition of high tariffs on Chinese goods has triggered short-term strains on China’s exports.At the same time,it has also accelerated its strategic pivot toward technological self-reliance,regional integration,and domestic demand expansion.展开更多
China’s five-year plans crystallize a governance model that merges long-term strategic vision with adaptive execution.AS China prepares to unveil its 15th Five-Year Plan in 2026,policymakers,investors,and scholars ar...China’s five-year plans crystallize a governance model that merges long-term strategic vision with adaptive execution.AS China prepares to unveil its 15th Five-Year Plan in 2026,policymakers,investors,and scholars around the world are watching closely.For over 70 years,these plans have guided the country’s economic and social development.展开更多
In 2012,we published the first special issue on the mechanisms of pain and itch in Neuroscience Bulletin[1],covering peripheral[2,3],central[4],and glial[5]mechanisms.In 2018,the second special issue expanded on these...In 2012,we published the first special issue on the mechanisms of pain and itch in Neuroscience Bulletin[1],covering peripheral[2,3],central[4],and glial[5]mechanisms.In 2018,the second special issue expanded on these topics[6],featuring single-cell profiling and in vivo Ca2+imaging of primary sensory neurons[7,8],and illustrating how nociceptors regulate pain,itch,and infection[9].It also highlighted spinal neurocircuits of pain[10]and itch[11],glial contributions[12],sex differences[13],and supraspinal mechanisms underlying pain and empathy[14,15].Over the past seven years,significant advances have been made in neuroglial and neuroimmune interactions and supraspinal circuits.Thus,this third special issue—comprising one review,eleven original articles,and one research highlight[16,17,18,19,20,21,22,23,24,25,26,27,28]—timely summarizes recent progress in pain and itch research.展开更多
Purpose:The purpose of this narrative review is to:1)summarize findings from the three Studies of a Targeted Risk Reduction Intervention through Defined Exercise(STRRIDE)randomized trials regarding the differential ef...Purpose:The purpose of this narrative review is to:1)summarize findings from the three Studies of a Targeted Risk Reduction Intervention through Defined Exercise(STRRIDE)randomized trials regarding the differential effects of exercise amount,intensity,and mode on metabolic syndrome(MetS);and 2)compare the STRRIDE findings with other published randomized exercise trials related to changes in MetS.Methods:A literature review was performed to investigate the effects of exercise on composite measures of MetS.PubMed was searched between October 2023 and December 2023.To be included in this review,studies must have employed a randomized study design,whereby exercise amount,intensity,or mode was varied.Results:Findings from the STRRIDE trials and other randomized exercise trials suggest:1)there is a relationship between exercise energy expenditure(ExEE)and improvements in composite measures of MetS;2)there may be an asymptotic effect for ExEE beyond which further improvements in MetS are negligible or counterproductive;3)improvements in composite measures of MetS are closely linked to insulin sensitivity;and 4)without controlling for total ExEE,combined aerobic and resistance training interventions offer the most robust improvements for composite MetS outcomes compared to either mode alone.Conclusion:Additional,large-scale,randomized exercise trials should be designed to investigate the potential asymptotic effect and associated threshold for ExEE,the interaction between exercise intensity and baseline insulin sensitivity,and the independent effects of exercise mode on MetS.展开更多
To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of...To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of the autophagy related protein(ATG) CaATG8c, a core component in autophagy. However, the involvement of CaBBX9 in both autophagy and heat tolerance remains unclear. In this study, we further confirmed the interaction between CaBBX9 and CaATG8c and defined the interaction regions of CaBBX9 as CONSTANS, CONSTANS-Like, and TOC1(CCT) domain and the fragment region. The expression of CaBBX9 can be induced by heat treatment. CaBBX9 is co-localized with CaATG8c in the nucleus and exhibits a transcriptional activity. When the expression of CaBBX9 is silenced, the heat tolerance of pepper is enhanced, shown by the decrement of MDA content, H2O2and dead cells accumulation, and relative electrolyte leakage, along with the increment of chlorophyll content and expression level of heat-tolerance-related genes. Overexpression of CaBBX9 in tomatoes displays the opposite effects. Taken together, we demonstrate that CaBBX9 negatively regulates the heat tolerance of peppers by exacerbating oxidative damage and inhibiting the expression of heat-related genes. Our findings provide a new clue for guiding crop breeding for pepper tolerance to heat stress.展开更多
This paper examines the design and optimization of optical fibers for high-speed data transmission, emphasizing advancements that maximize efficiency in modern communication networks. Optical fibers, core components o...This paper examines the design and optimization of optical fibers for high-speed data transmission, emphasizing advancements that maximize efficiency in modern communication networks. Optical fibers, core components of global communication infrastructure, are capable of transmitting data over long distances with minimal loss through principles like total internal reflection. This study explores single-mode and multi-mode fiber designs, providing an overview of key parameters such as core diameter, refractive index profile, and numerical aperture. Mathematical modeling using Maxwell’s equations plays a central role in optimizing fiber performance, helping engineers mitigate challenges like attenuation and dispersion. The paper also discusses advanced techniques, including dense wavelength division multiplexing (DWDM), which enables terabit-per-second data rates. Case studies in practical applications, such as fiber-to-the-home (FTTH) networks and transoceanic cables, highlight the impact of optimized designs on network performance. Looking forward, innovations in photonic crystal fibers and hollow-core fibers are expected to drive further improvements, enabling ultra-high-speed data transmission. The paper concludes by underscoring the significance of continuous research and development to address challenges in optical fiber technology and support the increasing demands of global communication systems.展开更多
Background:Bacterial pneumonia remains a leading cause of morbidity and mortality worldwide despite the widespread availability of antibiotics.Novel pneumonia therapies and biomarkers are urgently needed to improve ou...Background:Bacterial pneumonia remains a leading cause of morbidity and mortality worldwide despite the widespread availability of antibiotics.Novel pneumonia therapies and biomarkers are urgently needed to improve outcomes and advance personalized therapy.Using an established baboon model of S.pneumoniae pneumonia,we sought to characterize the temporal dynamics of pneumonia host responses to identify novel potential diagnostic and therapeutic molecular targets.Methods:We performed whole blood transcriptomics,unbiased proteomics,and peripheral cytokine measurements serially in baboons inoculated with S.pneumoniae(n=23)or saline(n=10)and modeled the peripheral blood host response using principal components analysis and complex sparse logistic regression.Differentially expressed genes were analyzed for pathway analysis.Results:Inoculated animals developed characteristic signs and symptoms of pneumonia.A 39-gene signature was derived that classified S.pneumoniae infection with high accuracy(auROC 0.9 and 0.99 at 24 and 48 h post-inoculation,respectively).Similar performance was observed for 48-h biomarker signatures derived from peripheral blood plasma proteomic and cytokine measurements(both auROC>0.9).The gene signature retained strong diagnostic performance(auROC=0.88)when transformed to human orthologs and applied to patients with acute respiratory illness(n=34)or healthy controls(n=20).Pathway analysis at 48 h identified downregulation of mitophagy and glucocorticoid signaling in peripheral blood.Conclusions:We report novel peripheral blood gene and protein expression signatures of S.pneumoniae pneumonia that could improve pneumonia diagnosis and found distinct pathways that may be amenable to modulation.Our findings illustrate how non-human primate models of bacterial pneumonia can successfully translate biomarker discoveries to patients.展开更多
Point-of-care ultrasonography(POCUS)is increasingly recognized as a valuable extension of the physical exam,offering real-time bedside insights to support clinical decision-making.In nephrology,lung ultrasound(LUS)is ...Point-of-care ultrasonography(POCUS)is increasingly recognized as a valuable extension of the physical exam,offering real-time bedside insights to support clinical decision-making.In nephrology,lung ultrasound(LUS)is gaining prominence for its ability to assess extravascular lung water and guide fluid management,especially in patients with end-stage renal disease.This narrative review highlights current applications,technical aspects,and limitations of LUS in nephrology.Studies such as the Lung Water by Ultrasound-Guided Treatment in Haemodialysis Patients trial indicate that LUS-guided ultrafiltration may help improve blood pressure control,reduce pulmonary congestion and acute heart failure events in dialysis patients.Simplified approaches like the 8-zone protocol have shown diagnostic accuracy comparable to the traditional 28-zone method,improving feasibility in clinical practice.Nonetheless,limitations exist,including reduced specificity in non-cardiogenic lung conditions and under recognition of right-sided congestion when used in isolation.A comprehensive hemodynamic assessment requires integrating LUS with inferior vena cava ultrasound,focused cardiac ultrasound,and venous Doppler.Successful implementation depends on structured training and an understanding of potential interpretation challenges.Looking ahead,streamlined protocols,multimodal integration,and standardized training will be key to establishing POCUS as a core tool in nephrology.展开更多
This paper analyzes North American shifts in renewable energy finance and building energy technologies from 2022 to 2025.Fueled by net-zero targets and policy incentives,clean energy investment now surpasses fossil fu...This paper analyzes North American shifts in renewable energy finance and building energy technologies from 2022 to 2025.Fueled by net-zero targets and policy incentives,clean energy investment now surpasses fossil fuel spending.We examine key financial tools—green bonds,corporate power purchase agreements(PPAs),public-private partnerships,and tax credits—and parallel advances in smart meters,grid-interactive efficient buildings,battery storage,heat pumps,and net-zero construction.Through case studies of municipal retrofit financing,integrated smart homes,and net-zero campuses,we illustrate emerging finance-technology-policy ecosystems poised to accelerate the energy transition and bolster climate resilience.展开更多
基金supported by a Borden Scholars awardDuke Claude D.Pepper Older Americans Independence Center Pilot Award(P30AG028716)by the NIH/NIA(R01AG081393)。
文摘Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hepatic ApoE expression(ΔApoE),24-month-oldΔApoE mice displayed a 95%reduction in circulating ApoE levels and significantly improved fracture healing.ApoE treatment of aged BMSCs inhibited osteoblast differentiation in tissue culture models;RNA-seq,Western blot,immunofluorescence,and RT-PCR analyses indicated that the Wnt/β-catenin pathway is the target of this inhibition.Indeed,we showed that ApoE had no effect on cultures with stabilizedβ-catenin levels.Next,we determined that Lrp4 serves as the osteoblast cell surface receptor to ApoE,as expression of Lrp4 is required in ApoE-based inhibition of Wnt/β-catenin signaling and osteoblast differentiation.Importantly,we validated this ApoE-Lrp4-Wnt/β-catenin molecular mechanism in human osteoblast differentiation.Finally,we identified an ApoE-neutralizing antibody(NAb)and used it to treat aged,wildtype mice 3 days after fracture surgery resulting in fracture calluses with 35%more bone deposition.Our work here identifies novel liver-to-bone cross-talk and a noninvasive,translatable therapeutic intervention for aged bone regeneration.
文摘Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh)regulates NSC quiescence and differentiation within neurogenic niches,glioblastoma cells exploit th ese pathways to enhance their adaptability and invasiveness.The involvement of muscarinic(M3)and nicotinic(α7)receptors in both cell types suggests that glioblastoma retains neural progenitor-like traits,contributing to its plasticity and resilience.This article explores the shared cholinergic mechanisms between NSCs and GSCs,highlighting their role in both neural development and glioblastoma progression.
文摘British Prime Minister Keir Starmer’s visit to China underscores economic cooperation and strategic stability amid global uncertainties.U.K.Prime Minister Keir Starmer’s visit to China in January marks a significant shift of Britain toward a more pragmatic and stability-oriented approach in Sino-British relations.After years of political tensions and limited high-level engagement,this trip underscores London’s intent to rebuild ties based on mutual economic and strategic interests.
基金supported by grants from the China Postdoctoral Science Foundation(2023M742867)the High-level Innovation and Entrepreneurship Talents Program of QinChuangYuan,Shaanxi Province,China(QCYRCXM-2022-234)the National Key R&D Program of China(2019YFD1000203)。
文摘Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remains largely unknown.Lateral organ boundary(LOB)domain(LBD)proteins are pivotal regulators of plant growth and development.To date,certain LOB/LBD transcription factors are seemingly implicated in fruit softening.In this study,we identified 42 LOB/LBD genes in the peach genome.Expression analysis showed a significant upregulation of PpLOB1 transcripts toward peach fruit ripening.PpLOB1 was classified into Class II subgroup,and showed high sequence similarity to several softening-related LOB/LBD transcription factors.Transient transformation assays showed that PpLOB1 positively modulates peach softening.Further experiments demonstrated that PpLOB1 directly targets and activates the promoters of pectate lyase 1(PpPL1)and PpPL15,thereby contributing to the regulation of fruit softening.Additionally,PpNAP4 up-regulates PpLOB1 expression by binding to its promoter.Meanwhile,our findings revealed that PpNAP4 and PpNAP6 cooperatively modulate the expression of Pp LOB1.Taken together,our findings revealed a novel regulatory module involving PpNAP4 and PpLOB1 that modulates peach fruit softening.
文摘The application of deep learning in fabric defect detection has become increasingly widespread.To address false positives and false negatives in fabric roll seam detection,and to improve automation efficiency and product quality,we propose the Multi-scale Context DeepLabV3+(MSC-DeepLabV3+),a semantic segmentation network designed for fabric roll seam detection,based on DeepLabV3+.The model improvements include enhancing the backbone performance through optimization of the UIB-MobileNetV2 network;designing the Dynamic Atrous and Sliding-window Fusion(DASF)module to improve adaptability to multi-scale seam structures with dynamic dilation rates and a sliding-window mechanism;and utilizing the Progressive Low-level Feature Fusion(PLFF)module to progressively restore seam boundary details via shallow feature fusion.Additionally,an enhanced 3-SE attention mechanism is employed,replacing the direct concatenation operation.Experimental results show thatMSCDeepLabV3+outperforms classical and recent segmentation models.Compared to DeepLabV3+with an Xception backbone,MSC-DeepLabV3+achieves a mean intersection over union(mIoU)of 92.30%and the boundary Fscore(BF)of 92.54%,representing improvements of 3.04%and 3.14%,respectively.Moreover,the model complexity is significantly reduced,with the model parameters(params)decreasing to 3.44M and Frames Per Second(FPS)increasing from 101 to 273,demonstrating its potential for deployment in resource-constrained industrial scenarios.
基金supported by the Science Fund of NPU-Duke China Seeds Program(Grant No.119003067)the CAST-BISEE Fund(Grant No.MC010175)+1 种基金the Project of National Natural Science Foundation of China(Grant No.12372233)the“111”project of China(Grant No.B17037).
文摘This study explores the nonlinear resonance of a rotating solar sail membrane exposed to time-varying solar thermal and solar radiation pressure.The sail membrane is modeled using a cantilever membrane,applying the von Kármán theory for membrane large deflection.The membrane’s nonlinear equation is derived by employing the Lagrange equation while accounting for excitations from solar thermal and radiation pressure.The equation is solved via the Rayleigh-Ritz method.The bifurcation diagram of membrane motion is applied to reveal membrane resonance responses under different solar sail rotating frequencies.The displacement time history,phase portrait,Poincarémap,frequency spectrum,and the largest Lyapunov exponent are used to study nonlinear vibrations that occur near resonance regions.The results indicate that time-varying thermal loading excites membrane motions with multiple natural frequencies by the parametric resonance mechanics,leading to the onset of membrane chaotic motion.The membrane’s primary resonance is stimulated in harmonic oscillation by the time-varying radiation pressure.The divergence instability caused by thermal excitation is also illustrated by comparing the membrane’s vibration amplitude with and without thermal excitation.The membrane’s nonlinear vibration characteristics vary significantly with solar illumination angles,the membrane’s thermal expansion coefficients,and structural damping.
基金supported by the Czech Science Foundation(projects 24-11371S to TM and 2306840S to MAL)Masaryk University Grant Agency(project MUNI/R/1268/2022 to TM)+1 种基金part of a long-term research project of the Czech Academy of Sciences,Institute of Botany(RVO 67985939)provided by the ELIXIR-CZ project(LM2023055),part of the international ELIXIR infrastructure。
文摘The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments.One such species,B.calcarea,has been described from the Chandalaz Mountains in northeastern Asia(Russia).This study aimed to investigate the genome structure and evolutionary history of B.calcarea.However,our analyses reveal that the species does not belong to Boechera.Instead,an integrative approach combining cytogenetic,phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae.The diploid Parryodes calcarea(2n=16)exhibits Arabideae-specific chromosomal signatures,including multiple centromere repositionings.These findings clarify the misclassification of P.calcarea as Boechera,leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World.This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe,the Arabideae.
文摘Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation of these new neurons in the olfactory bulb supports both structural and functional plasticity, aiding in circuit remodeling triggered by memory and learning processes. However, the presence of these neurons, coupled with the cellular diversity within the olfactory bulb, presents an ongoing challenge in understanding its network organization and function. Moreover,the continuous integration of new neurons in the olfactory bulb plays a pivotal role in regulating olfactory information processing. This adaptive process responds to changes in epithelial composition and contributes to the formation of olfactory memories by modulating cellular connectivity within the olfactory bulb and interacting intricately with higher-order brain regions. The role of adult neurogenesis in olfactory bulb functions remains a topic of debate. Nevertheless, the functionality of the olfactory bulb is intricately linked to the organization of granule cells around mitral and tufted cells. This organizational pattern significantly impacts output, network behavior, and synaptic plasticity, which are crucial for olfactory perception and memory. Additionally, this organization is further shaped by axon terminals originating from cortical and subcortical regions. Despite the crucial role of olfactory bulb in brain functions and behaviors related to olfaction, these complex and highly interconnected processes have not been comprehensively studied as a whole. Therefore, this manuscript aims to discuss our current understanding and explore how neural plasticity and olfactory neurogenesis contribute to enhancing the adaptability of the olfactory system. These mechanisms are thought to support olfactory learning and memory, potentially through increased complexity and restructuring of neural network structures, as well as the addition of new granule granule cells that aid in olfactory adaptation. Additionally, the manuscript underscores the importance of employing precise methodologies to elucidate the specific roles of adult neurogenesis amidst conflicting data and varying experimental paradigms. Understanding these processes is essential for gaining insights into the complexities of olfactory function and behavior.
文摘BACKGROUND Coronavirus disease 2019(COVID-19)disrupted healthcare and led to increased telehealth use.We explored the impact of COVID-19 on liver transplant evaluation(LTE).AIM To understand the impact of telehealth on LTE during COVID-19 and to identify disparities in outcomes disaggregated by sociodemographic factors.METHODS This was a retrospective study of patients who initiated LTE at our center from 3/16/20-3/16/21(“COVID-19 era”)and the year prior(3/16/19-3/15/20,“pre-COVID-19 era”).We compared LTE duration times between eras and explored the effects of telehealth and inpatient evaluations on LTE duration,listing,and pretransplant mortality.RESULTS One hundred and seventy-eight patients were included in the pre-COVID-19 era cohort and one hundred and ninety-nine in the COVID-19 era cohort.Twentynine percent(58/199)of COVID-19 era initial LTE were telehealth,compared to 0%(0/178)pre-COVID-19.There were more inpatient evaluations during COVID-19 era(40%vs 28%,P<0.01).Among outpatient encounters,telehealth use for initial LTE during COVID-19 era did not impact likelihood of listing,pretransplant mortality,or time to LTE and listing.Median times to LTE and listing during COVID-19 were shorter than pre-COVID-19,driven by increased inpatient evaluations.Sociodemographic factors were not predictive of telehealth.CONCLUSION COVID-19 demonstrates a shift to telehealth and inpatient LTE.Telehealth does not impact LTE or listing duration,likelihood of listing,or mortality,suggesting telehealth may facilitate LTE without negative outcomes.
文摘Appreciation of soft-tissue thickness(STT)at surgical sites is an increasingly recognized aspect of arthroplasty procedures as it may potentially impacting postoperative outcomes.Recent research has focused on the predictive value of preoperative STT measurements for complications following various forms of arthroplasty,particularly infections,across procedures such as total knee,hip,shoulder,and ankle replacements.Several studies have indicated that increased STT is associated with a higher risk of complications,including infection and wound healing issues.The assessment of STT before surgery could play a crucial role in identifying patients at a higher risk of complications and may be instru-mental in guiding preoperative planning to optimize outcomes in arthroplasty procedures.Standardized measurement techniques and further research are essential to enhance the reliability and clinical utility of STT assessment for arthro-plasty surgery.
文摘The U.S.imposition of high tariffs on Chinese goods has triggered short-term strains on China’s exports.At the same time,it has also accelerated its strategic pivot toward technological self-reliance,regional integration,and domestic demand expansion.
文摘China’s five-year plans crystallize a governance model that merges long-term strategic vision with adaptive execution.AS China prepares to unveil its 15th Five-Year Plan in 2026,policymakers,investors,and scholars around the world are watching closely.For over 70 years,these plans have guided the country’s economic and social development.
文摘In 2012,we published the first special issue on the mechanisms of pain and itch in Neuroscience Bulletin[1],covering peripheral[2,3],central[4],and glial[5]mechanisms.In 2018,the second special issue expanded on these topics[6],featuring single-cell profiling and in vivo Ca2+imaging of primary sensory neurons[7,8],and illustrating how nociceptors regulate pain,itch,and infection[9].It also highlighted spinal neurocircuits of pain[10]and itch[11],glial contributions[12],sex differences[13],and supraspinal mechanisms underlying pain and empathy[14,15].Over the past seven years,significant advances have been made in neuroglial and neuroimmune interactions and supraspinal circuits.Thus,this third special issue—comprising one review,eleven original articles,and one research highlight[16,17,18,19,20,21,22,23,24,25,26,27,28]—timely summarizes recent progress in pain and itch research.
基金Leanna M.Ross is supported by the American Heart Association Career Development Award 23CDA1051777National Institute on Aging grant 5P30AG028716-18。
文摘Purpose:The purpose of this narrative review is to:1)summarize findings from the three Studies of a Targeted Risk Reduction Intervention through Defined Exercise(STRRIDE)randomized trials regarding the differential effects of exercise amount,intensity,and mode on metabolic syndrome(MetS);and 2)compare the STRRIDE findings with other published randomized exercise trials related to changes in MetS.Methods:A literature review was performed to investigate the effects of exercise on composite measures of MetS.PubMed was searched between October 2023 and December 2023.To be included in this review,studies must have employed a randomized study design,whereby exercise amount,intensity,or mode was varied.Results:Findings from the STRRIDE trials and other randomized exercise trials suggest:1)there is a relationship between exercise energy expenditure(ExEE)and improvements in composite measures of MetS;2)there may be an asymptotic effect for ExEE beyond which further improvements in MetS are negligible or counterproductive;3)improvements in composite measures of MetS are closely linked to insulin sensitivity;and 4)without controlling for total ExEE,combined aerobic and resistance training interventions offer the most robust improvements for composite MetS outcomes compared to either mode alone.Conclusion:Additional,large-scale,randomized exercise trials should be designed to investigate the potential asymptotic effect and associated threshold for ExEE,the interaction between exercise intensity and baseline insulin sensitivity,and the independent effects of exercise mode on MetS.
基金supported by a grant from the National Natural Science Foundation of China (32172552, 31572114)the earmarked fund for the China Agriculture Research System (CARS23-G22)。
文摘To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of the autophagy related protein(ATG) CaATG8c, a core component in autophagy. However, the involvement of CaBBX9 in both autophagy and heat tolerance remains unclear. In this study, we further confirmed the interaction between CaBBX9 and CaATG8c and defined the interaction regions of CaBBX9 as CONSTANS, CONSTANS-Like, and TOC1(CCT) domain and the fragment region. The expression of CaBBX9 can be induced by heat treatment. CaBBX9 is co-localized with CaATG8c in the nucleus and exhibits a transcriptional activity. When the expression of CaBBX9 is silenced, the heat tolerance of pepper is enhanced, shown by the decrement of MDA content, H2O2and dead cells accumulation, and relative electrolyte leakage, along with the increment of chlorophyll content and expression level of heat-tolerance-related genes. Overexpression of CaBBX9 in tomatoes displays the opposite effects. Taken together, we demonstrate that CaBBX9 negatively regulates the heat tolerance of peppers by exacerbating oxidative damage and inhibiting the expression of heat-related genes. Our findings provide a new clue for guiding crop breeding for pepper tolerance to heat stress.
文摘This paper examines the design and optimization of optical fibers for high-speed data transmission, emphasizing advancements that maximize efficiency in modern communication networks. Optical fibers, core components of global communication infrastructure, are capable of transmitting data over long distances with minimal loss through principles like total internal reflection. This study explores single-mode and multi-mode fiber designs, providing an overview of key parameters such as core diameter, refractive index profile, and numerical aperture. Mathematical modeling using Maxwell’s equations plays a central role in optimizing fiber performance, helping engineers mitigate challenges like attenuation and dispersion. The paper also discusses advanced techniques, including dense wavelength division multiplexing (DWDM), which enables terabit-per-second data rates. Case studies in practical applications, such as fiber-to-the-home (FTTH) networks and transoceanic cables, highlight the impact of optimized designs on network performance. Looking forward, innovations in photonic crystal fibers and hollow-core fibers are expected to drive further improvements, enabling ultra-high-speed data transmission. The paper concludes by underscoring the significance of continuous research and development to address challenges in optical fiber technology and support the increasing demands of global communication systems.
基金Bill and Melinda Gates Foundation,Grant/Award Number:OPP1017554。
文摘Background:Bacterial pneumonia remains a leading cause of morbidity and mortality worldwide despite the widespread availability of antibiotics.Novel pneumonia therapies and biomarkers are urgently needed to improve outcomes and advance personalized therapy.Using an established baboon model of S.pneumoniae pneumonia,we sought to characterize the temporal dynamics of pneumonia host responses to identify novel potential diagnostic and therapeutic molecular targets.Methods:We performed whole blood transcriptomics,unbiased proteomics,and peripheral cytokine measurements serially in baboons inoculated with S.pneumoniae(n=23)or saline(n=10)and modeled the peripheral blood host response using principal components analysis and complex sparse logistic regression.Differentially expressed genes were analyzed for pathway analysis.Results:Inoculated animals developed characteristic signs and symptoms of pneumonia.A 39-gene signature was derived that classified S.pneumoniae infection with high accuracy(auROC 0.9 and 0.99 at 24 and 48 h post-inoculation,respectively).Similar performance was observed for 48-h biomarker signatures derived from peripheral blood plasma proteomic and cytokine measurements(both auROC>0.9).The gene signature retained strong diagnostic performance(auROC=0.88)when transformed to human orthologs and applied to patients with acute respiratory illness(n=34)or healthy controls(n=20).Pathway analysis at 48 h identified downregulation of mitophagy and glucocorticoid signaling in peripheral blood.Conclusions:We report novel peripheral blood gene and protein expression signatures of S.pneumoniae pneumonia that could improve pneumonia diagnosis and found distinct pathways that may be amenable to modulation.Our findings illustrate how non-human primate models of bacterial pneumonia can successfully translate biomarker discoveries to patients.
文摘Point-of-care ultrasonography(POCUS)is increasingly recognized as a valuable extension of the physical exam,offering real-time bedside insights to support clinical decision-making.In nephrology,lung ultrasound(LUS)is gaining prominence for its ability to assess extravascular lung water and guide fluid management,especially in patients with end-stage renal disease.This narrative review highlights current applications,technical aspects,and limitations of LUS in nephrology.Studies such as the Lung Water by Ultrasound-Guided Treatment in Haemodialysis Patients trial indicate that LUS-guided ultrafiltration may help improve blood pressure control,reduce pulmonary congestion and acute heart failure events in dialysis patients.Simplified approaches like the 8-zone protocol have shown diagnostic accuracy comparable to the traditional 28-zone method,improving feasibility in clinical practice.Nonetheless,limitations exist,including reduced specificity in non-cardiogenic lung conditions and under recognition of right-sided congestion when used in isolation.A comprehensive hemodynamic assessment requires integrating LUS with inferior vena cava ultrasound,focused cardiac ultrasound,and venous Doppler.Successful implementation depends on structured training and an understanding of potential interpretation challenges.Looking ahead,streamlined protocols,multimodal integration,and standardized training will be key to establishing POCUS as a core tool in nephrology.
文摘This paper analyzes North American shifts in renewable energy finance and building energy technologies from 2022 to 2025.Fueled by net-zero targets and policy incentives,clean energy investment now surpasses fossil fuel spending.We examine key financial tools—green bonds,corporate power purchase agreements(PPAs),public-private partnerships,and tax credits—and parallel advances in smart meters,grid-interactive efficient buildings,battery storage,heat pumps,and net-zero construction.Through case studies of municipal retrofit financing,integrated smart homes,and net-zero campuses,we illustrate emerging finance-technology-policy ecosystems poised to accelerate the energy transition and bolster climate resilience.
