BACKGROUND Globally,complete neurological recovery of spinal cord injury(SCI)is still less than 1%,and 90%experience permanent disability.The key issue is that a pharmacological neuroprotective-neuroregenerative agent...BACKGROUND Globally,complete neurological recovery of spinal cord injury(SCI)is still less than 1%,and 90%experience permanent disability.The key issue is that a pharmacological neuroprotective-neuroregenerative agent and SCI regeneration mechanism have not been found.The secretomes of stem cell are an emerging neurotrophic agent,but the effect of human neural stem cells(HNSCs)secretome on SCI is still unclear.AIM To investigate the regeneration mechanism of SCI and neuroprotective-neuroregenerative effects of HNSCs-secretome on subacute SCI post-laminectomy in rats.METHODS An experimental study was conducted with 45 Rattus norvegicus,divided into 15 normal,15 control(10 mL physiologic saline),and 15 treatment(30μL HNSCssecretome,intrathecal T10,three days post-traumatic).Locomotor function was evaluated weekly by blinded evaluators.Fifty-six days post-injury,specimens were collected,and spinal cord lesion,free radical oxidative stress(F2-Isoprostanes),nuclear factor-kappa B(NF-κB),matrix metallopeptidase 9(MMP9),tumor necrosis factor-alpha(TNF-α),interleukin-10(IL-10),transforming growth factor-beta(TGF-β),vascular endothelial growth factor(VEGF),B cell lymphoma-2(Bcl-2),nestin,brain-derived neurotrophic factor(BDNF),glial cell line-derived neurotrophic factor(GDNF)were analyzed.The SCI regeneration mechanism was analyzed using partial least squares structural equation modeling(PLS SEM).RESULTS HNSCs-secretome significantly improved locomotor recovery according to Basso,Beattie,Bresnahan(BBB)scores and increased neurogenesis(nestin,BDNF,and GDNF),neuroangiogenesis(VEGF),anti-apoptotic(Bcl-2),anti-inflammatory(IL-10 and TGF-β),but decreased proinflammatory(NF-κB,MMP9,TNF-α),F2-Isoprostanes,and spinal cord lesion size.The SCI regeneration mechanism is valid by analyzed outer model,inner model,and hypothesis testing in PLS SEM,started with pro-inflammation followed by anti-inflammation,anti-apoptotic,neuroangiogenesis,neurogenesis,and locomotor function.CONCLUSION HNSCs-secretome as a potential neuroprotective-neuroregenerative agent for the treatment of SCI and uncover the SCI regeneration mechanism.展开更多
Objective:Endometriosis is a chronic gynecological disease requiring relatively long therapy of at least 3 to 6 months,and has a high recurrence rate.Further research using animal models is needed to better understand...Objective:Endometriosis is a chronic gynecological disease requiring relatively long therapy of at least 3 to 6 months,and has a high recurrence rate.Further research using animal models is needed to better understand the disease.During the COVID-19 pandemic,laparoscopic surgeries were suspended to minimize infection risk.This study aims to establish an experimental animal model of endometriosis using stored chocolate cyst pulp.Methods:This laboratory experimental study included 12 female Mus musculus mice.Immunodeficient mice were intraperitoneally injected with a previously prepared chocolate cyst slurry.On the 15th day,the mice were euthanized,and anatomical pathological examination was performed using hematoxylin and eosin(HE)staining.Results:Anatomical pathology examination revealed stromatosis in the fat tissue of all 12 mice treated with stored chocolate cyst slurry,confirming the presence of endometriosis.The protocol demonstrated a 100%success rate in developing a mouse model of endometriosis.Conclusion:The successful development of a mouse endometriosis model from stored chocolate cyst slurry using this protocol is expected to contribute to experimental animal studies.展开更多
文摘BACKGROUND Globally,complete neurological recovery of spinal cord injury(SCI)is still less than 1%,and 90%experience permanent disability.The key issue is that a pharmacological neuroprotective-neuroregenerative agent and SCI regeneration mechanism have not been found.The secretomes of stem cell are an emerging neurotrophic agent,but the effect of human neural stem cells(HNSCs)secretome on SCI is still unclear.AIM To investigate the regeneration mechanism of SCI and neuroprotective-neuroregenerative effects of HNSCs-secretome on subacute SCI post-laminectomy in rats.METHODS An experimental study was conducted with 45 Rattus norvegicus,divided into 15 normal,15 control(10 mL physiologic saline),and 15 treatment(30μL HNSCssecretome,intrathecal T10,three days post-traumatic).Locomotor function was evaluated weekly by blinded evaluators.Fifty-six days post-injury,specimens were collected,and spinal cord lesion,free radical oxidative stress(F2-Isoprostanes),nuclear factor-kappa B(NF-κB),matrix metallopeptidase 9(MMP9),tumor necrosis factor-alpha(TNF-α),interleukin-10(IL-10),transforming growth factor-beta(TGF-β),vascular endothelial growth factor(VEGF),B cell lymphoma-2(Bcl-2),nestin,brain-derived neurotrophic factor(BDNF),glial cell line-derived neurotrophic factor(GDNF)were analyzed.The SCI regeneration mechanism was analyzed using partial least squares structural equation modeling(PLS SEM).RESULTS HNSCs-secretome significantly improved locomotor recovery according to Basso,Beattie,Bresnahan(BBB)scores and increased neurogenesis(nestin,BDNF,and GDNF),neuroangiogenesis(VEGF),anti-apoptotic(Bcl-2),anti-inflammatory(IL-10 and TGF-β),but decreased proinflammatory(NF-κB,MMP9,TNF-α),F2-Isoprostanes,and spinal cord lesion size.The SCI regeneration mechanism is valid by analyzed outer model,inner model,and hypothesis testing in PLS SEM,started with pro-inflammation followed by anti-inflammation,anti-apoptotic,neuroangiogenesis,neurogenesis,and locomotor function.CONCLUSION HNSCs-secretome as a potential neuroprotective-neuroregenerative agent for the treatment of SCI and uncover the SCI regeneration mechanism.
文摘Objective:Endometriosis is a chronic gynecological disease requiring relatively long therapy of at least 3 to 6 months,and has a high recurrence rate.Further research using animal models is needed to better understand the disease.During the COVID-19 pandemic,laparoscopic surgeries were suspended to minimize infection risk.This study aims to establish an experimental animal model of endometriosis using stored chocolate cyst pulp.Methods:This laboratory experimental study included 12 female Mus musculus mice.Immunodeficient mice were intraperitoneally injected with a previously prepared chocolate cyst slurry.On the 15th day,the mice were euthanized,and anatomical pathological examination was performed using hematoxylin and eosin(HE)staining.Results:Anatomical pathology examination revealed stromatosis in the fat tissue of all 12 mice treated with stored chocolate cyst slurry,confirming the presence of endometriosis.The protocol demonstrated a 100%success rate in developing a mouse model of endometriosis.Conclusion:The successful development of a mouse endometriosis model from stored chocolate cyst slurry using this protocol is expected to contribute to experimental animal studies.
