Background: Breast cancer is a type of cancer that affects more women throughout the world, in developing anddeveloped countries. 2-AEH2P is a phospholipid analog of cellular membrane, which makes it different from ex...Background: Breast cancer is a type of cancer that affects more women throughout the world, in developing anddeveloped countries. 2-AEH2P is a phospholipid analog of cellular membrane, which makes it different from existing molecules fortheir absorption, stability and display anti-inflammatory, anti-proliferative and pro-apoptotic properties. Methods: MCF-7 humanbreast adenocarcinoma cells were treated with 2-AEH2P. The viability and adhesion cells were evaluated by MTT assay. Cell cyclephases, apoptosis, markers and mitochondrial potential were assessed by flow cytometry. Morphological ultrastructural analyzeswere performed by laser confocal microscopy. Results: MCF-7 Tumor cells acquired round shapes, lost cytoplasmic expansions,formed clusters in suspension and decreased significantly viability. There were changes in the morphology, membrane fragmentationand loss of cytoplasmic projection. The obtained concentrations for IC50% were 37.2;25.8;1.8 mM for periods of 24, 48 and 72 h,respectively. Changes in the distribution of cell population phases of the cell cycle showed an increase in fragmented DNA and anincrease in the G2/M phase. The expression β-gal showed proliferative reduction induced by 2-AEH2P. Laser confocal microscopyshowed changes in the mitochondrial membrane and alteration in distribution. Proliferative index of MCF-7 tumor cells treated with2-AEH2P decreased significantly when compared to fibroblast normal cells. The compound 2-AEH2P is a phospholipid withantiproliferative potential and apoptosis modulator.展开更多
User authentication on smart devices is crucial to protecting user privacy and device security.Due to the development of emerging attacks,existing physiological feature-based authentication methods,such as fingerprint...User authentication on smart devices is crucial to protecting user privacy and device security.Due to the development of emerging attacks,existing physiological feature-based authentication methods,such as fingerprint,iris,and face recognition are vulnerable to forgery and attacks.In this paper,GestureID,a system that utilizes acoustic sensing technology to distinguish hand features among users,is proposed.It involves using a speaker to send acoustic signals and a microphone to receive the echoes affected by the reflection of the hand movements of the users.To ensure system accuracy and effectively distinguish users’gestures,a second-order differential-based phase extraction method is proposed.This method calculates the gradient of received signals to separate the effects of the user’s hand movements on the transmitted signal from the background noise.Then,the secondorder differential phase and phase-dependent acceleration information are used as inputs to a Convolutional Neural Networks-Bidirectional Long Short-Term Memory(CNN-BiLSTM)model to model hand motion features.To decrease the time it takes to collect data for new user registration,a transfer learning method is used.This involves creating a user authentication model by utilizing a pre-trained gesture recognition model.As a result,accurate user authentication can be achieved without requiring extensive amounts of training data.Experiments demonstrate that GestureID can achieve 97.8%gesture recognition accuracy and 96.3%user authentication accuracy.展开更多
The animal has the potential to serve as a clinical model for human disease,due to striking similarities and homologies in diseases.Early clinical development of a new drug may influence its final destination,and a ca...The animal has the potential to serve as a clinical model for human disease,due to striking similarities and homologies in diseases.Early clinical development of a new drug may influence its final destination,and a careful and thoughtful approach to Phase-1 clinical trials is essential.Phosphorylethanolamine(2-AEH2P)is a new cytostatic phospholipid agent that,unlike most chemotherapeutic drugs used today,does not target DNA or cytoskeleton,but act on the cell membrane.Studies carried out by our group have shown several effects of 2-AEH2P controlling the progression of the cell cycle,in the alterations of the mitochondrial electrical potential capable of inducing apoptosis and autophagy in several types of human and animal tumor cells.The aim of this study is to establish the recommended safety dose of a 2-AEH2P monophosphoester,in dogs with tumor following the Phase 1 study model proposed by Fibonacci.The Phase-1 study following the Fibonacci model,2-AEH2P was safe at all staggered doses up to 150 mg/Kg for 8 weeks.Intravenous administration in staggered doses according to the Fibonacci model,showed that the drug is safe,no advent of mortality during the study period or acute toxicity were observed.It is not a drug with hemolytic properties or that induces anemia.It does not lead to changes in liver and renal functions and was able to modulate leukocyte production.2-AEH2P is new compound with antitumor potential,being useful for future veterinary and human tumors,as a combination of chemotherapeutic agents.展开更多
基金Fundação de Amparo a Pesquisa do Estado de São Paulo–FAPESP(Process number 2014/02344-1)Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq(Process number 305056/2019-0).
文摘Background: Breast cancer is a type of cancer that affects more women throughout the world, in developing anddeveloped countries. 2-AEH2P is a phospholipid analog of cellular membrane, which makes it different from existing molecules fortheir absorption, stability and display anti-inflammatory, anti-proliferative and pro-apoptotic properties. Methods: MCF-7 humanbreast adenocarcinoma cells were treated with 2-AEH2P. The viability and adhesion cells were evaluated by MTT assay. Cell cyclephases, apoptosis, markers and mitochondrial potential were assessed by flow cytometry. Morphological ultrastructural analyzeswere performed by laser confocal microscopy. Results: MCF-7 Tumor cells acquired round shapes, lost cytoplasmic expansions,formed clusters in suspension and decreased significantly viability. There were changes in the morphology, membrane fragmentationand loss of cytoplasmic projection. The obtained concentrations for IC50% were 37.2;25.8;1.8 mM for periods of 24, 48 and 72 h,respectively. Changes in the distribution of cell population phases of the cell cycle showed an increase in fragmented DNA and anincrease in the G2/M phase. The expression β-gal showed proliferative reduction induced by 2-AEH2P. Laser confocal microscopyshowed changes in the mitochondrial membrane and alteration in distribution. Proliferative index of MCF-7 tumor cells treated with2-AEH2P decreased significantly when compared to fibroblast normal cells. The compound 2-AEH2P is a phospholipid withantiproliferative potential and apoptosis modulator.
基金funded by the Science and Technology Research Program of Henan Province of China(No.182102210130)“Research on Key Technologies of Practical Quantum-Resistant Authenticated Key Agreement Protocols”.
文摘User authentication on smart devices is crucial to protecting user privacy and device security.Due to the development of emerging attacks,existing physiological feature-based authentication methods,such as fingerprint,iris,and face recognition are vulnerable to forgery and attacks.In this paper,GestureID,a system that utilizes acoustic sensing technology to distinguish hand features among users,is proposed.It involves using a speaker to send acoustic signals and a microphone to receive the echoes affected by the reflection of the hand movements of the users.To ensure system accuracy and effectively distinguish users’gestures,a second-order differential-based phase extraction method is proposed.This method calculates the gradient of received signals to separate the effects of the user’s hand movements on the transmitted signal from the background noise.Then,the secondorder differential phase and phase-dependent acceleration information are used as inputs to a Convolutional Neural Networks-Bidirectional Long Short-Term Memory(CNN-BiLSTM)model to model hand motion features.To decrease the time it takes to collect data for new user registration,a transfer learning method is used.This involves creating a user authentication model by utilizing a pre-trained gesture recognition model.As a result,accurate user authentication can be achieved without requiring extensive amounts of training data.Experiments demonstrate that GestureID can achieve 97.8%gesture recognition accuracy and 96.3%user authentication accuracy.
基金Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq).Durvanei Augusto Maria-Process number 305056/2019-0.
文摘The animal has the potential to serve as a clinical model for human disease,due to striking similarities and homologies in diseases.Early clinical development of a new drug may influence its final destination,and a careful and thoughtful approach to Phase-1 clinical trials is essential.Phosphorylethanolamine(2-AEH2P)is a new cytostatic phospholipid agent that,unlike most chemotherapeutic drugs used today,does not target DNA or cytoskeleton,but act on the cell membrane.Studies carried out by our group have shown several effects of 2-AEH2P controlling the progression of the cell cycle,in the alterations of the mitochondrial electrical potential capable of inducing apoptosis and autophagy in several types of human and animal tumor cells.The aim of this study is to establish the recommended safety dose of a 2-AEH2P monophosphoester,in dogs with tumor following the Phase 1 study model proposed by Fibonacci.The Phase-1 study following the Fibonacci model,2-AEH2P was safe at all staggered doses up to 150 mg/Kg for 8 weeks.Intravenous administration in staggered doses according to the Fibonacci model,showed that the drug is safe,no advent of mortality during the study period or acute toxicity were observed.It is not a drug with hemolytic properties or that induces anemia.It does not lead to changes in liver and renal functions and was able to modulate leukocyte production.2-AEH2P is new compound with antitumor potential,being useful for future veterinary and human tumors,as a combination of chemotherapeutic agents.
