The European Standard EN 1504 ＂Products and systems for protection and repair of concrete structures -- Definitions, requirements, quality control and evaluation of conformity＂ can be considered a real milestone for...The European Standard EN 1504 ＂Products and systems for protection and repair of concrete structures -- Definitions, requirements, quality control and evaluation of conformity＂ can be considered a real milestone for the companies involved in the rehabilitation of buildings, bridges, piers, pipelines, and other civil or industrial concrete structures. However in some cases the examination and, even more, the practical application of the proposed guidelines are made difficult by a complicated sub-division in principles and methods, and by a confusing and repetitive layout. The paper deals in particular with the principles and methods for the rehabilitation of concrete damage due to reinforcement corrosion, often strictly connected, anyway, to those adopted in order to rehabilitate the concrete itself. Suggestions are given for a possible future update of the Standard, based on the personal experience of the Authors.展开更多
Colitis is a common disease of the colon that is very difficult to treat.Probiotic bacteria could be an effective treatment.The probiotic Escherichia coli Nissle 1917(EcN)was engineered to synthesize the ketone body(R...Colitis is a common disease of the colon that is very difficult to treat.Probiotic bacteria could be an effective treatment.The probiotic Escherichia coli Nissle 1917(EcN)was engineered to synthesize the ketone body(R)-3-hydroxybutyrate(3HB)for sustainable production in the gut lumen of mice suffering from colitis.Components of heterologous 3HB synthesis routes were constructed,expressed,optimized,and inserted into the EcN genome,combined with deletions in competitive branch pathways.The genome-engineered EcN produced the highest 3HB level of 0.6 g/L under microaerobic conditions.The live therapeutic was found to colonize the mouse gastrointestinal tract over 14 days,elevating gut 3HB and short-chain-length fatty acid(SCFA)levels 8.7-and 3.1-fold compared to those of wild-type EcN,respectively.The sustainable presence of 3HB in mouse guts promoted the growth of probiotic bacteria,especially Akkermansia spp.,to over 31%from the initial 2%of all the microbiome.As a result,the engineered EcN termed EcNL4 ameliorated colitis induced via dextran sulfate sodium(DSS)in mice.Compared to wild-type EcN or oral administration of 3HB,oral EcNL4 uptake demonstrated better effects on mouse weights,colon lengths,occult blood levels,gut tissue myeloperoxidase activity and proinflammatory cytokine concentrations.Thus,a promising live bacterium was developed to improve colonic microenvironments and further treat colitis.This proof-of-concept design can be employed to treat other diseases of the colon.展开更多
基金National Key Fundamental Research Program (No.2004CCA07300) National Natural Science Foundation of China (No.20176017)+1 种基金 National Hi-tech Research and Development Program (No.AA514020-02) Anhui Excellent Youth Science and Technology Foundation (No.04044059)
文摘The European Standard EN 1504 ＂Products and systems for protection and repair of concrete structures -- Definitions, requirements, quality control and evaluation of conformity＂ can be considered a real milestone for the companies involved in the rehabilitation of buildings, bridges, piers, pipelines, and other civil or industrial concrete structures. However in some cases the examination and, even more, the practical application of the proposed guidelines are made difficult by a complicated sub-division in principles and methods, and by a confusing and repetitive layout. The paper deals in particular with the principles and methods for the rehabilitation of concrete damage due to reinforcement corrosion, often strictly connected, anyway, to those adopted in order to rehabilitate the concrete itself. Suggestions are given for a possible future update of the Standard, based on the personal experience of the Authors.
基金This research was financially supported by a grant from the Chunfeng Foundation(2020Z99CFG002)of Tsinghua UniversityOther support includes the National Natural Science Foundation of China(Grant Nos.31870859,21761132013,31771886,and 31971170).
文摘Colitis is a common disease of the colon that is very difficult to treat.Probiotic bacteria could be an effective treatment.The probiotic Escherichia coli Nissle 1917(EcN)was engineered to synthesize the ketone body(R)-3-hydroxybutyrate(3HB)for sustainable production in the gut lumen of mice suffering from colitis.Components of heterologous 3HB synthesis routes were constructed,expressed,optimized,and inserted into the EcN genome,combined with deletions in competitive branch pathways.The genome-engineered EcN produced the highest 3HB level of 0.6 g/L under microaerobic conditions.The live therapeutic was found to colonize the mouse gastrointestinal tract over 14 days,elevating gut 3HB and short-chain-length fatty acid(SCFA)levels 8.7-and 3.1-fold compared to those of wild-type EcN,respectively.The sustainable presence of 3HB in mouse guts promoted the growth of probiotic bacteria,especially Akkermansia spp.,to over 31%from the initial 2%of all the microbiome.As a result,the engineered EcN termed EcNL4 ameliorated colitis induced via dextran sulfate sodium(DSS)in mice.Compared to wild-type EcN or oral administration of 3HB,oral EcNL4 uptake demonstrated better effects on mouse weights,colon lengths,occult blood levels,gut tissue myeloperoxidase activity and proinflammatory cytokine concentrations.Thus,a promising live bacterium was developed to improve colonic microenvironments and further treat colitis.This proof-of-concept design can be employed to treat other diseases of the colon.
