AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and patholog...AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and pathological features of primary hepatocellular carcinoma (HCC). METHODS: Forty-seven HCC and ten normal liver specimens were collected during surgical operation between 2001 and 2003. FHIT and proliferating cell nuclear antigen (PCNA) expression were detected by immunohistochemistry, and apoptotic level was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay on the tissue sections. RESULTS: All normal liver tissues showed a strong expression of FHIT, whereas 28 of 47 (59.6%) carcinomas showed a significant loss or absence of FHIT expression (P= 0.001). The proportion of reduced FHIT expression in those carcinomas at stages Ⅲ-Ⅳ (70.6%) and in those with extrahepatic metastasis (86.7%) showed an increasing trend compared with those at stages HI (30.8%, P= 0.013) and those without metastasis (46.9%, P = 0.010) respectively. Apoptotic incidence in advanced TNM stage carcinoma and those with positive FHIT expression was higher than that in early stage carcinoma (P=0.030) and in those with negative FHIT expression (P=0.044) respectively. The proliferating potential of hepatocellular carcinoma was associated with FHIT expression (P= 0.016) and the aggressive feature (P = 0.019). Kaplan-Meier analysis demonstrated that the survival time of these 47 patients correlated with TNM stage, FHIT expression and metastasis. CONCLUSION: There is marked loss or absence of FHIT expression, as well as abnormal apoptosis-prdiferation balance in HCC. FHIT may play an important role in carcinogenesis and development of HCC.展开更多
AIM: To discuss the angiographic features of gastrointestinastromal tumor (GIST) and to evaluate their diagnostic role.METHODS: Twelve patients with pathologically proved GISTunderwent angiography (DSA)I wk before ope...AIM: To discuss the angiographic features of gastrointestinastromal tumor (GIST) and to evaluate their diagnostic role.METHODS: Twelve patients with pathologically proved GISTunderwent angiography (DSA)I wk before operation, usingPuck and digital subtraction DSA. The origin, size, morphologyand angiographic appearance of the lesions were reviewed.RESULTS: Two tumors arose from stomach, 8 from jejunum,and 2 from ileum. Seven cases were benign and 5 weremalignant. Obviously thickened supplying arteries weredetected in 8 tumors, and early-developed veins were foundin 3. Two types of angiographic changes of GIST were observed.Four cases had twisted irregular neoplastic vessels withpartially coarse and indistinct margins, which were allmalignant. Eight cases had ball-like neoplastic vessels withuniform tumor staining, of which 7 were benign and 1 wasmalignant.CONCLUSION: Angiography facilitates localization anddiagnosis of GIST, helps define their size, range and location,and is especially valuable to patients suffering from melenawith unknown reasons.展开更多
文摘AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, product of a candidate tumor suppressor, and to investigate the relationship between FHIT, cell apoptosis and proliferation, and pathological features of primary hepatocellular carcinoma (HCC). METHODS: Forty-seven HCC and ten normal liver specimens were collected during surgical operation between 2001 and 2003. FHIT and proliferating cell nuclear antigen (PCNA) expression were detected by immunohistochemistry, and apoptotic level was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay on the tissue sections. RESULTS: All normal liver tissues showed a strong expression of FHIT, whereas 28 of 47 (59.6%) carcinomas showed a significant loss or absence of FHIT expression (P= 0.001). The proportion of reduced FHIT expression in those carcinomas at stages Ⅲ-Ⅳ (70.6%) and in those with extrahepatic metastasis (86.7%) showed an increasing trend compared with those at stages HI (30.8%, P= 0.013) and those without metastasis (46.9%, P = 0.010) respectively. Apoptotic incidence in advanced TNM stage carcinoma and those with positive FHIT expression was higher than that in early stage carcinoma (P=0.030) and in those with negative FHIT expression (P=0.044) respectively. The proliferating potential of hepatocellular carcinoma was associated with FHIT expression (P= 0.016) and the aggressive feature (P = 0.019). Kaplan-Meier analysis demonstrated that the survival time of these 47 patients correlated with TNM stage, FHIT expression and metastasis. CONCLUSION: There is marked loss or absence of FHIT expression, as well as abnormal apoptosis-prdiferation balance in HCC. FHIT may play an important role in carcinogenesis and development of HCC.
文摘AIM: To discuss the angiographic features of gastrointestinastromal tumor (GIST) and to evaluate their diagnostic role.METHODS: Twelve patients with pathologically proved GISTunderwent angiography (DSA)I wk before operation, usingPuck and digital subtraction DSA. The origin, size, morphologyand angiographic appearance of the lesions were reviewed.RESULTS: Two tumors arose from stomach, 8 from jejunum,and 2 from ileum. Seven cases were benign and 5 weremalignant. Obviously thickened supplying arteries weredetected in 8 tumors, and early-developed veins were foundin 3. Two types of angiographic changes of GIST were observed.Four cases had twisted irregular neoplastic vessels withpartially coarse and indistinct margins, which were allmalignant. Eight cases had ball-like neoplastic vessels withuniform tumor staining, of which 7 were benign and 1 wasmalignant.CONCLUSION: Angiography facilitates localization anddiagnosis of GIST, helps define their size, range and location,and is especially valuable to patients suffering from melenawith unknown reasons.
