AIM: To investigate the value of transabdominal ulbasonography (US) in the preoperative staging of gastric cancer.METHODS: A total of 198 patients with gastric cancer underwent preoperatively transabdominal US, depth...AIM: To investigate the value of transabdominal ulbasonography (US) in the preoperative staging of gastric cancer.METHODS: A total of 198 patients with gastric cancer underwent preoperatively transabdominal US, depth of tumor infiltration was assessed in 125 patients, and lymph node metastasis was assessed in 106 patients,RESULTS: The staging accuracy of transabdominal US was 55.6%, 75.0%, 87.3% and 71.1% in T1, T2, T3 and T4 carcinomas, respectively. The overall accuracy was 77.6%.The detection rate for pancreatic invasion and liver invasion was 77.4%, 71.4%, respectively. The sensitivity, specificity,accuracy of transabdominal US in assessment of lymph node metastasis were 77.6%, 64.1%, 72.6%, respectively.Various shapes such as round, ovoid, spindle were encountered in benign and malignant lymph nodes. Majority of boa benign and malignant lymph nodes were hyperechoic and had a distinct border. Benign lymph nodes were smaller than malignant lymph nodes in length and width (P = 0.000,0.005). Irregular shape, fusional shape, infiltrative signs,inhomogenous echo were seen mainly in malignant lymph nodes (P = 0.045, 0.006, 0.027, 0.006).CONCLUSION: Transabdominal US is useful for preoperative staging in gastric cancer, although it is difficult to differentiate benign from malignant lymph nodes.展开更多
AIM: To establish a nude mice model of human hepatocellular carcinoma (HCC) via orthotopic implantation of histologically intact tissue, in order to study biologic features of HCC in vivo and to direct clinical treatm...AIM: To establish a nude mice model of human hepatocellular carcinoma (HCC) via orthotopic implantation of histologically intact tissue, in order to study biologic features of HCC in vivo and to direct clinical treatment respectively.METHODS: Histologically intact fresh specimens of HCC were orthotopically implanted in nude mice (BALB/c, nu/nu). Survival rate and growth curve were investigated with Bultrasound. Morphological characteristics of pathology and spontaneous metastatic rates were detected with microscopy. Expression of multidrug resistance genes studied with immunohistochemical method and RT-PCR, and other biologic features of implanted tumor were observed and compared with human HCC specimens. RESULTS: Out of the specimens from two patients with HCC, only one specimen survived in nude mice. The orthotopic implantation tumor survival rate, spontaneous intrahepatic metastatic rate, pulmonary metastatic rate and bone metastases rate were 100%, 75.0%, 37.5% and 37.5% respectively in the first passage. AFP was kept on secreting and increasing with the size of the tumor. The morphological characteristics and biologic features were similar to the donor's, the protein and mRNA of MDR1 and LRP were expressed in tumors of the model and the donor, and there was no significant difference between them (P>0.05). CONCLUTION: The model of nude mice with orthotopic implantation of histologically intact HCC tissue is an ideal model to study biologic features of HCC in vivo and to direct clinical treatment.展开更多
AIM: To verify the expressing efficiency and angiogenesiseffect after administration of expression vector encodingfor vascular endothelial growth factor D in normal andischemic rat liver.METHODS: Ten female S-D rats w...AIM: To verify the expressing efficiency and angiogenesiseffect after administration of expression vector encodingfor vascular endothelial growth factor D in normal andischemic rat liver.METHODS: Ten female S-D rats were administrated withliver tissue dot injection of naked PCHO/hVEGF-D, 50 μg/dot, three dots for each. The same amount of physiologicalsaline was used as control in the neighboring lobe. ForteenS-D rats, using inflow occlusion of left lateral lobe, were dividedinto two groups, seven rats in each group. One was ischemicplasmid group, which received naked plasmid PCHO/hVEGF-D injection of 150 μg. The other received the equal amountof natural saline injection and designed as control. Theexpressions of hVEGF-D in mRNA and protein levels wereidentified by in situ hybridyzation and immunohistochemistry,respectively. Endothelial cells were labeled by the factor VIIIimmunohistochemistrically. The average number of peri-sinusoidal capillaries of each group was calculated andcompared statistically 8 days after injection.RESULTS: A large amount of hVEGF-D in mRNA level wasfound in both normal and ischemic plasmid groups and butnone in their corresponding control groups. The protein ofhVEGF was also highly expressed in both normal and ischemicplasmid groups than in the controls. The mean number ofcapillaries under microscopy (×200) of the plasmid group andcontrol was 10.2±2.78 vs7.1±2.02 (P＜0.05), and those ofischemic plasmid group and ischemic control were 7.43+1.72vs 4.71± 1.11 with statistical difference (P＜0.05).CONCLUSION; The naked PCHO/hVEGF-D dot injection tonormal, ischemic rat liver can produce comparatively highexpression of hVEGF in both protein and mRNA levels, andprominently increase the number of new capillaries aroundhepatic sinuses. Therefore, it could be another ideal choicefor the treatment of ischemic liver diseases.展开更多
87 patients with pulmonary space-occupied lesions of the peripheral type which were either adhesive of close to pleura were examined using ultuasonography.Of them,64 cases of lung cancers and 23 of bening lesions were...87 patients with pulmonary space-occupied lesions of the peripheral type which were either adhesive of close to pleura were examined using ultuasonography.Of them,64 cases of lung cancers and 23 of bening lesions were then confirmed by展开更多
基金Supported by theHospital Medicine Development Foundation,No.ZD 199909
文摘AIM: To investigate the value of transabdominal ulbasonography (US) in the preoperative staging of gastric cancer.METHODS: A total of 198 patients with gastric cancer underwent preoperatively transabdominal US, depth of tumor infiltration was assessed in 125 patients, and lymph node metastasis was assessed in 106 patients,RESULTS: The staging accuracy of transabdominal US was 55.6%, 75.0%, 87.3% and 71.1% in T1, T2, T3 and T4 carcinomas, respectively. The overall accuracy was 77.6%.The detection rate for pancreatic invasion and liver invasion was 77.4%, 71.4%, respectively. The sensitivity, specificity,accuracy of transabdominal US in assessment of lymph node metastasis were 77.6%, 64.1%, 72.6%, respectively.Various shapes such as round, ovoid, spindle were encountered in benign and malignant lymph nodes. Majority of boa benign and malignant lymph nodes were hyperechoic and had a distinct border. Benign lymph nodes were smaller than malignant lymph nodes in length and width (P = 0.000,0.005). Irregular shape, fusional shape, infiltrative signs,inhomogenous echo were seen mainly in malignant lymph nodes (P = 0.045, 0.006, 0.027, 0.006).CONCLUSION: Transabdominal US is useful for preoperative staging in gastric cancer, although it is difficult to differentiate benign from malignant lymph nodes.
基金Supported by the Science and Technology Special Fund of Ministry of Public Health,No.WKZ-2000-1-15 the Key Clinic Programs of Ministry of Public Health,No.2001-2003
文摘AIM: To establish a nude mice model of human hepatocellular carcinoma (HCC) via orthotopic implantation of histologically intact tissue, in order to study biologic features of HCC in vivo and to direct clinical treatment respectively.METHODS: Histologically intact fresh specimens of HCC were orthotopically implanted in nude mice (BALB/c, nu/nu). Survival rate and growth curve were investigated with Bultrasound. Morphological characteristics of pathology and spontaneous metastatic rates were detected with microscopy. Expression of multidrug resistance genes studied with immunohistochemical method and RT-PCR, and other biologic features of implanted tumor were observed and compared with human HCC specimens. RESULTS: Out of the specimens from two patients with HCC, only one specimen survived in nude mice. The orthotopic implantation tumor survival rate, spontaneous intrahepatic metastatic rate, pulmonary metastatic rate and bone metastases rate were 100%, 75.0%, 37.5% and 37.5% respectively in the first passage. AFP was kept on secreting and increasing with the size of the tumor. The morphological characteristics and biologic features were similar to the donor's, the protein and mRNA of MDR1 and LRP were expressed in tumors of the model and the donor, and there was no significant difference between them (P>0.05). CONCLUTION: The model of nude mice with orthotopic implantation of histologically intact HCC tissue is an ideal model to study biologic features of HCC in vivo and to direct clinical treatment.
基金the National Science Fund for Postdoctoral Fellows in China,No 2001.6the Medical Science Found of Shandong Province,No.2001CA1DBA10
文摘AIM: To verify the expressing efficiency and angiogenesiseffect after administration of expression vector encodingfor vascular endothelial growth factor D in normal andischemic rat liver.METHODS: Ten female S-D rats were administrated withliver tissue dot injection of naked PCHO/hVEGF-D, 50 μg/dot, three dots for each. The same amount of physiologicalsaline was used as control in the neighboring lobe. ForteenS-D rats, using inflow occlusion of left lateral lobe, were dividedinto two groups, seven rats in each group. One was ischemicplasmid group, which received naked plasmid PCHO/hVEGF-D injection of 150 μg. The other received the equal amountof natural saline injection and designed as control. Theexpressions of hVEGF-D in mRNA and protein levels wereidentified by in situ hybridyzation and immunohistochemistry,respectively. Endothelial cells were labeled by the factor VIIIimmunohistochemistrically. The average number of peri-sinusoidal capillaries of each group was calculated andcompared statistically 8 days after injection.RESULTS: A large amount of hVEGF-D in mRNA level wasfound in both normal and ischemic plasmid groups and butnone in their corresponding control groups. The protein ofhVEGF was also highly expressed in both normal and ischemicplasmid groups than in the controls. The mean number ofcapillaries under microscopy (×200) of the plasmid group andcontrol was 10.2±2.78 vs7.1±2.02 (P＜0.05), and those ofischemic plasmid group and ischemic control were 7.43+1.72vs 4.71± 1.11 with statistical difference (P＜0.05).CONCLUSION; The naked PCHO/hVEGF-D dot injection tonormal, ischemic rat liver can produce comparatively highexpression of hVEGF in both protein and mRNA levels, andprominently increase the number of new capillaries aroundhepatic sinuses. Therefore, it could be another ideal choicefor the treatment of ischemic liver diseases.
文摘87 patients with pulmonary space-occupied lesions of the peripheral type which were either adhesive of close to pleura were examined using ultuasonography.Of them,64 cases of lung cancers and 23 of bening lesions were then confirmed by
