AIM: Gastroesophageal reflux disease (GERD) is a common disorder in the Western population, but detailed population-based data in China are limited. The aim of this study was to understand the epidemiology of symptoma...AIM: Gastroesophageal reflux disease (GERD) is a common disorder in the Western population, but detailed population-based data in China are limited. The aim of this study was to understand the epidemiology of symptomatic gastroesophageal reflux (SGER) in adults of Xi'an, a northwestern city of China, and to explore the potential risk factors of GERD. METHODS: Symptoms suggestive of GERD, functional dyspepsia (FD), irritable bowel syndrome (IBS), upper respiratory diseases and some potential risk factors were investigated in a face-to-face manner in a region-stratified random samples of 2 789 residents aged 18-70 years in Xi'an by using a standardized questionnaire. METHODS: With a response rate of 91.8%, the prevalence of SGER was 16.98% (95% CI, 14.2-18.92) in Xi'an adults, and no gender-related difference was observed (P<0.05). SGER was more common among subjects aged 30-70 years than in those aged 18-29 years (P<0.01). The prevalence of SGER in rural, urban and suburban subjects was 21.07%, 17.44% and 12.12%, respectively, and there was a significant difference between rural, urban and suburban regions (P<0.05). Compared with subjects without SGER, the prevalence of symptoms suggestive of FD and IBS, pneumonia, asthma, bronchitis, laryngitis, pharyngitis, chronic cough, wheeze, globus sensation, oral ulcer and snore was significantly increased in subjects with SGER (P<0.01). Heavy smoking (OR=5.76; CI, 3.70-6.67), heavy alcohol use (OR=2.85; CI, 1.67-4.49), peplJc ulcer (OR=5.76; CI, 3.99-8.32), cerebral palsy (OR=3.97; CI, 1.97-8.00), abdominal operation (OR=2.69; CI, 1.75-4.13), obesity(OR=2.16; CI, 1.47-3.16), excessive food intake (OR= 1.43;CI, 1.17-1.15), sweet food (OR=1.23; CI, 0.89-1.54), and consumption of coffee (OR= 1.23; CI, 0.17-2.00) were independently associated with SGER. The episodes of GERD were commonly precipitated by dietary factors (66.05%), followed by body posture (26.54%), ill temper (23.72%), fatigue (22.32%) and stress (10.93%). CONCLUSION: GERD is common in Xi'an's adult population with a mild or moderate degree. The etiology and pathogenesis of GERD are probably associated with FD, IBS, and some respiratory, laryngopharyngeal and odontostological diseases or symptoms. Some lifestyles, diseases and dietary factors are the risk factors of GERD.展开更多
AIM: To construct a phage display library of human singlechain variable fragment (scFv) antibodies associated with esophageal cancer and to preliminarily screen a scFvantibody against esophageal cancer.METHODS: Total ...AIM: To construct a phage display library of human singlechain variable fragment (scFv) antibodies associated with esophageal cancer and to preliminarily screen a scFvantibody against esophageal cancer.METHODS: Total RNA extracted from metastatic lymph nodes of esophageal cancer patients was used to construct a scFv gene library. Rescued by M13K07 helper phage, the scFv phage display library was constructed, esophageal cancer cell line Eca 109 and normal human esophageal epithelial cell line (NHEEC) were used for panning and subtractive panning of the scFv phage display library to obtain positive phage clones. Soluble scFv was expressed in E.coliHB2151 which was transfected with the positive phage clone, then purified by affinity chromatography.Relative molecular mass of soluble scFv was estimated by Western blotting, its bioactivity was detected by cell ELISA assay. Sequence of scFv was determined using the method of dideoxynucleotide sequencing.RESULTS: The size of scFv gene library was approximately 9×10^6 clones. After four rounds of panning with Eca109 and three rounds of subtractive panning with NHEEC cells,25 positive phage clones were obtained. Soluble scFv was found to have a molecular mass of 31 ku and was able to bind to Ecal09 cells, but not to HeLa and NHEEC cells.Variable heavy (VH) gene from one of the positive clones was shown to be derived from the γ chain subgroup IV of immunoglobulin, and variable light (VL) gene from the κ chain subgroup I of immunoglobulin.CONCLUSION: A human scFv phage display library can be constructed from the metastatic lymph nodes of esophageal cancer patients. A whole human scFv against esophageal cancer shows some bioactivity.展开更多
AIM:This paper aims to develop a data-based semi-quantitative food frequency questionnaire (SQFFQ) covering both urban and rural areas in the Chaoshan region of Guangdong Province, China, for the investigation of rela...AIM:This paper aims to develop a data-based semi-quantitative food frequency questionnaire (SQFFQ) covering both urban and rural areas in the Chaoshan region of Guangdong Province, China, for the investigation of relationships between food intake and lifestyle-related diseases among middle-aged Chinese. METHODS: We recruited 417 subjects from the general population and performed an assessment of the diet, using a 3-d weighed dietary record survey. We employed contribution analysis (CA) and multiple regression analysis (MRA) to select food items covering up to a 90% contribution and a 0.90 R2, respectively. The total number of food items consumed was 523 (443 in the urban and 417 in the rural population) and the intake of 29 nutrients was calculated according to the actual consumption by foods/ recipes. RESULTS: The CA selected 233,194, and 183 foods/recipes for the combined, the urban and the rural areas, respectively, and then 196,157, and 160 were chosen by the MRA. Finally, 125 foods/recipes were selected for the final questionnaire. The frequencies were classified into eight categories and standard portion sizes were also calculated. CONCLUSION: For adoption of the area-specific SQFFQ, validity and reproducibility tests are now planned tc determine how the combined SQFFQ performs in actual assessment of disease risk and benefit.展开更多
Determination of soil Se by anodic stripping voltammetry (ASV) with aurum thin-film electrode (ATFE) over-comes the interference of gold peak with selenium peak, and thus has a higher sensitivity with the mininiumdete...Determination of soil Se by anodic stripping voltammetry (ASV) with aurum thin-film electrode (ATFE) over-comes the interference of gold peak with selenium peak, and thus has a higher sensitivity with the mininiumdetectable concentration being 0.017 μg/mL, the standard deviation of the measured results less than 0.012 μg/g,the coefficient of variation lower than 10%, and the recovery rate between 86% to 103%. Besides the measurementconditions, the digestion of soil sample was also studied in detail.展开更多
AIM: To study the effect of manganese (Mn) on heat stressprotein 70 (HSP70) synthesis in the brain and liver of new-bom rats whose mother-rats were exposed to Mn.METHODS: 32 female rats were randomly divided into four...AIM: To study the effect of manganese (Mn) on heat stressprotein 70 (HSP70) synthesis in the brain and liver of new-bom rats whose mother-rats were exposed to Mn.METHODS: 32 female rats were randomly divided into fourgroups. One group was administrated with physiologicalsaline only as control group, the other three groups wereadministrated with 7.5, 15 and 30 mg@ kg-1 manganesechloride (MnCl2) by intraperitioneal injection every two daysfor two weeks. After delivery, the mother-rats receivedMnCl2 unceasingly for a week with the same method. Thenthe contents of Mn、 Zn、 Cu and Fe in the livers of the new-bom rats were determined by atomic absorptionspectroscopy; The level of HSP70 in the brains and thelivers of the new-born rats as detected by Westsrn-dot-blotting, and the SOD activities were measuredsimultaneously.RESULTS: The contents of Mn in the livers of new-bom ratsof the experimental groups(respective 1.38 ± 0.18, 2.73 ±0.65, 3.44 ± 0.89μg @ g-1) were significantly increasedcompared with the control group(0.88 ± 0.18μg@ g-1; p ＜0.01); The contents of Fe in the livers of new-bom rats of 15and 30 mg@ kg-1 experimental groups (426 ± 125,572 ± 175μg@g-1, respectively) were significantly increased comparedwith the control group(286±42μg@g-1; P＜0.05); the levelsof Zn in the livers of the new-bom rats of three experimentalgroups( 254 ± 49, 263 ± 47, 213 ± 28μg@ g-1, respectively)were lower than those of the control group(335 ± 50μg@g-1;respective P＜0.05, P＜0.01); and the levels of Cu showedno significant difference among the four groups (threeexperimental groups: 75 ± 21, 68 ± 241 and 78 ± 18μg@g-1;control group: 83 ± 9μg@ g-1; p ＞ 0.05). There was asignificant increase in the levels of HSP70 in the brains ofnew-bom rats of the 30 mg@kg-1 group (19.5 × 103 ± 1.3 ×103A; control group: 14.3 × 103 ± 1.4 × 103A; P＜ 0.01),andthe levels of HSP70 in the livers of new-bom rats of threeexperinental groups(respective 19.6 × 103 ± 3.9 × 103A, 18.5× 103 ± 3.8 × 103A, 22.4 × 103 ± 1.9 × 103A ) also increasedthan control group(13.3 × 103 ± 1.0 × 103A; P ＜ 0.01), butthe SOD activities showed no significant difference amongbrains of the four groups (experimental groups: 5.04 ± 0.43,4.83±0.48, 4.60±0.84 ku@g-1; control group: 4.91 ± 0.37ku@g-1; P＞ 0.05). The SOD activities in the livers of 15mg@kg-1 group(5.41 ± 0.44 ku@g-1) was lower than the controlgroup(5.95±0.36 ku@g-1; P＜0.05).CONCLUSION: While mother-rats were exposed tomanganese, the metabolisms of Mn、Zn and Fe of new- bornrats in the livers were influenced and were situated in astress status, thus HSP70 syntheses is induced in the brainsand livers of new-bom rats, but the mechanism of this effectin the developmental toxicity of Mn remains to he furtherstudied.展开更多
Information Center for Public Health, Chinese Academy of preventive Medicine, Beijing 100050, China; △ Beijing Research Institute for TB Control, Beijing 100035, China The study investigated the bu...Information Center for Public Health, Chinese Academy of preventive Medicine, Beijing 100050, China; △ Beijing Research Institute for TB Control, Beijing 100035, China The study investigated the burden of smear positive pulmonary TB and its infectivity, using DALY (Disability Adjusted Life Year) as an indicator. An assumed cohort of 2000 cases was set up based on the age specific incidence of 794 newly registered smear positive cases of TB in Beijing in 1994. Prognostic trees and model diagrams of infectivity under natural history and DOTS (Direct Observed Treatment, Short course) strategy were established according to the epidemiological evidence. The results show that 29.6% of DALYs would be neglected if the burden caused by the infectivity was not considered. The results also show that DOTS strategy may reduce 97.3% of the number of potential cases infected, 92.9% of DALYs related to TB patients themselves, and 99.9% of DALYs caused by TB's infectivity as well.展开更多
The crystal structure of the title compound C16H18NO2+·C7H7SO3-·2H2O, (C23H29NSO7, Mr=463.53) has been determined by single-crystal X-ray diffraction analysis. The crystal belongs to the monoclinic system wi...The crystal structure of the title compound C16H18NO2+·C7H7SO3-·2H2O, (C23H29NSO7, Mr=463.53) has been determined by single-crystal X-ray diffraction analysis. The crystal belongs to the monoclinic system with space group P21/n, a=8.101(1), b=8.958(2), c=33.281(5)?,β= 94.910(1)(, V=2406.3(7)?3, Z=4, Dc=1.279g/cm3, μ=0.176mm-1, F(000)=984, final R=0.0409, and Rw=0.0860 for 4401 independent reflections. The result shows that in the crystal structure of the title compound the planar cations have two configurations with equal occupation ratio and are antiparally packed through π…π interactions. Similar packing energies in A and B are probably the main factor that leads to the disorder structure.展开更多
AIM: To ascertain the adequacy of the microsatellite instability (MSI) as a prognostic indicator by assessing MSI status of patients with double primary gastric and colorectal cancer (DPGCC).METHODS: Sixteen patients ...AIM: To ascertain the adequacy of the microsatellite instability (MSI) as a prognostic indicator by assessing MSI status of patients with double primary gastric and colorectal cancer (DPGCC).METHODS: Sixteen patients were studied, all of whom exhibited sporadic DPGCC, and had no family history of hereditary non-polyposis colorectal cancer, according to the Amsterdam criteria. A total of 32 cancers from 16DPGCC patients, and 216 single primary CRC, were assessed for MSI in 5 microsatellite loci, BAT25, BAT26,D2S123, D5S346, and D17S250.RESULTS: MSI was observed in 6 (37.5%) of 16 GC and 4 (25.0%) of 16 CRC. Thirty tumors (13.9%) out of 216single primary CRC and one tumor (16.7%) out of 6 double primary CRC were found to be microsatellite unstable. Of the 6 GC with MSI in DPGCC, 5 (31.3%) were MSI-high and one (6.3%) was MSI-low. In 5 of 16 DPGCC patients,the cancer recurred in or adjacent to the anastomosis or metastasized to the kidney or lung. The MSI-high DPGCC cases were associated with a younger age of onset (47.5 years vs 62.5 years), higher frequency of lymph node metastasis (100% vs 25%), and advanced Dukes stage (C, 100% vs 41.7%), as well as a higher frequency of recurrence or metastasis (100% vs 8.3%). Only recurrence or metastasis showed statistical significance by Fisher's exact test.CONCLUSION: Our data suggest that MSI may play an important role in the development of DPGCC, and that it may be used clinically as a molecular predictive marker for recurrence or late metastasis of DPGCC.展开更多
文摘AIM: Gastroesophageal reflux disease (GERD) is a common disorder in the Western population, but detailed population-based data in China are limited. The aim of this study was to understand the epidemiology of symptomatic gastroesophageal reflux (SGER) in adults of Xi'an, a northwestern city of China, and to explore the potential risk factors of GERD. METHODS: Symptoms suggestive of GERD, functional dyspepsia (FD), irritable bowel syndrome (IBS), upper respiratory diseases and some potential risk factors were investigated in a face-to-face manner in a region-stratified random samples of 2 789 residents aged 18-70 years in Xi'an by using a standardized questionnaire. METHODS: With a response rate of 91.8%, the prevalence of SGER was 16.98% (95% CI, 14.2-18.92) in Xi'an adults, and no gender-related difference was observed (P<0.05). SGER was more common among subjects aged 30-70 years than in those aged 18-29 years (P<0.01). The prevalence of SGER in rural, urban and suburban subjects was 21.07%, 17.44% and 12.12%, respectively, and there was a significant difference between rural, urban and suburban regions (P<0.05). Compared with subjects without SGER, the prevalence of symptoms suggestive of FD and IBS, pneumonia, asthma, bronchitis, laryngitis, pharyngitis, chronic cough, wheeze, globus sensation, oral ulcer and snore was significantly increased in subjects with SGER (P<0.01). Heavy smoking (OR=5.76; CI, 3.70-6.67), heavy alcohol use (OR=2.85; CI, 1.67-4.49), peplJc ulcer (OR=5.76; CI, 3.99-8.32), cerebral palsy (OR=3.97; CI, 1.97-8.00), abdominal operation (OR=2.69; CI, 1.75-4.13), obesity(OR=2.16; CI, 1.47-3.16), excessive food intake (OR= 1.43;CI, 1.17-1.15), sweet food (OR=1.23; CI, 0.89-1.54), and consumption of coffee (OR= 1.23; CI, 0.17-2.00) were independently associated with SGER. The episodes of GERD were commonly precipitated by dietary factors (66.05%), followed by body posture (26.54%), ill temper (23.72%), fatigue (22.32%) and stress (10.93%). CONCLUSION: GERD is common in Xi'an's adult population with a mild or moderate degree. The etiology and pathogenesis of GERD are probably associated with FD, IBS, and some respiratory, laryngopharyngeal and odontostological diseases or symptoms. Some lifestyles, diseases and dietary factors are the risk factors of GERD.
基金Supported by the National Natural Science Foundation of China,No.30070291 andNo.30213914 Medical Scientific Foundation of Guangdong Province,No.A2000434
文摘AIM: To construct a phage display library of human singlechain variable fragment (scFv) antibodies associated with esophageal cancer and to preliminarily screen a scFvantibody against esophageal cancer.METHODS: Total RNA extracted from metastatic lymph nodes of esophageal cancer patients was used to construct a scFv gene library. Rescued by M13K07 helper phage, the scFv phage display library was constructed, esophageal cancer cell line Eca 109 and normal human esophageal epithelial cell line (NHEEC) were used for panning and subtractive panning of the scFv phage display library to obtain positive phage clones. Soluble scFv was expressed in E.coliHB2151 which was transfected with the positive phage clone, then purified by affinity chromatography.Relative molecular mass of soluble scFv was estimated by Western blotting, its bioactivity was detected by cell ELISA assay. Sequence of scFv was determined using the method of dideoxynucleotide sequencing.RESULTS: The size of scFv gene library was approximately 9×10^6 clones. After four rounds of panning with Eca109 and three rounds of subtractive panning with NHEEC cells,25 positive phage clones were obtained. Soluble scFv was found to have a molecular mass of 31 ku and was able to bind to Ecal09 cells, but not to HeLa and NHEEC cells.Variable heavy (VH) gene from one of the positive clones was shown to be derived from the γ chain subgroup IV of immunoglobulin, and variable light (VL) gene from the κ chain subgroup I of immunoglobulin.CONCLUSION: A human scFv phage display library can be constructed from the metastatic lymph nodes of esophageal cancer patients. A whole human scFv against esophageal cancer shows some bioactivity.
基金Supported by the Science and Technology Project Foundation of Guangdong Province, No. 2003C33706Grant-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Science, Sports, Culture and Technology, Japan
文摘AIM:This paper aims to develop a data-based semi-quantitative food frequency questionnaire (SQFFQ) covering both urban and rural areas in the Chaoshan region of Guangdong Province, China, for the investigation of relationships between food intake and lifestyle-related diseases among middle-aged Chinese. METHODS: We recruited 417 subjects from the general population and performed an assessment of the diet, using a 3-d weighed dietary record survey. We employed contribution analysis (CA) and multiple regression analysis (MRA) to select food items covering up to a 90% contribution and a 0.90 R2, respectively. The total number of food items consumed was 523 (443 in the urban and 417 in the rural population) and the intake of 29 nutrients was calculated according to the actual consumption by foods/ recipes. RESULTS: The CA selected 233,194, and 183 foods/recipes for the combined, the urban and the rural areas, respectively, and then 196,157, and 160 were chosen by the MRA. Finally, 125 foods/recipes were selected for the final questionnaire. The frequencies were classified into eight categories and standard portion sizes were also calculated. CONCLUSION: For adoption of the area-specific SQFFQ, validity and reproducibility tests are now planned tc determine how the combined SQFFQ performs in actual assessment of disease risk and benefit.
文摘Determination of soil Se by anodic stripping voltammetry (ASV) with aurum thin-film electrode (ATFE) over-comes the interference of gold peak with selenium peak, and thus has a higher sensitivity with the mininiumdetectable concentration being 0.017 μg/mL, the standard deviation of the measured results less than 0.012 μg/g,the coefficient of variation lower than 10%, and the recovery rate between 86% to 103%. Besides the measurementconditions, the digestion of soil sample was also studied in detail.
基金Natural Science Foundation of Metallurgical Industry Ministry,No.(1996)254-21
文摘AIM: To study the effect of manganese (Mn) on heat stressprotein 70 (HSP70) synthesis in the brain and liver of new-bom rats whose mother-rats were exposed to Mn.METHODS: 32 female rats were randomly divided into fourgroups. One group was administrated with physiologicalsaline only as control group, the other three groups wereadministrated with 7.5, 15 and 30 mg@ kg-1 manganesechloride (MnCl2) by intraperitioneal injection every two daysfor two weeks. After delivery, the mother-rats receivedMnCl2 unceasingly for a week with the same method. Thenthe contents of Mn、 Zn、 Cu and Fe in the livers of the new-bom rats were determined by atomic absorptionspectroscopy; The level of HSP70 in the brains and thelivers of the new-born rats as detected by Westsrn-dot-blotting, and the SOD activities were measuredsimultaneously.RESULTS: The contents of Mn in the livers of new-bom ratsof the experimental groups(respective 1.38 ± 0.18, 2.73 ±0.65, 3.44 ± 0.89μg @ g-1) were significantly increasedcompared with the control group(0.88 ± 0.18μg@ g-1; p ＜0.01); The contents of Fe in the livers of new-bom rats of 15and 30 mg@ kg-1 experimental groups (426 ± 125,572 ± 175μg@g-1, respectively) were significantly increased comparedwith the control group(286±42μg@g-1; P＜0.05); the levelsof Zn in the livers of the new-bom rats of three experimentalgroups( 254 ± 49, 263 ± 47, 213 ± 28μg@ g-1, respectively)were lower than those of the control group(335 ± 50μg@g-1;respective P＜0.05, P＜0.01); and the levels of Cu showedno significant difference among the four groups (threeexperimental groups: 75 ± 21, 68 ± 241 and 78 ± 18μg@g-1;control group: 83 ± 9μg@ g-1; p ＞ 0.05). There was asignificant increase in the levels of HSP70 in the brains ofnew-bom rats of the 30 mg@kg-1 group (19.5 × 103 ± 1.3 ×103A; control group: 14.3 × 103 ± 1.4 × 103A; P＜ 0.01),andthe levels of HSP70 in the livers of new-bom rats of threeexperinental groups(respective 19.6 × 103 ± 3.9 × 103A, 18.5× 103 ± 3.8 × 103A, 22.4 × 103 ± 1.9 × 103A ) also increasedthan control group(13.3 × 103 ± 1.0 × 103A; P ＜ 0.01), butthe SOD activities showed no significant difference amongbrains of the four groups (experimental groups: 5.04 ± 0.43,4.83±0.48, 4.60±0.84 ku@g-1; control group: 4.91 ± 0.37ku@g-1; P＞ 0.05). The SOD activities in the livers of 15mg@kg-1 group(5.41 ± 0.44 ku@g-1) was lower than the controlgroup(5.95±0.36 ku@g-1; P＜0.05).CONCLUSION: While mother-rats were exposed tomanganese, the metabolisms of Mn、Zn and Fe of new- bornrats in the livers were influenced and were situated in astress status, thus HSP70 syntheses is induced in the brainsand livers of new-bom rats, but the mechanism of this effectin the developmental toxicity of Mn remains to he furtherstudied.
文摘Information Center for Public Health, Chinese Academy of preventive Medicine, Beijing 100050, China; △ Beijing Research Institute for TB Control, Beijing 100035, China The study investigated the burden of smear positive pulmonary TB and its infectivity, using DALY (Disability Adjusted Life Year) as an indicator. An assumed cohort of 2000 cases was set up based on the age specific incidence of 794 newly registered smear positive cases of TB in Beijing in 1994. Prognostic trees and model diagrams of infectivity under natural history and DOTS (Direct Observed Treatment, Short course) strategy were established according to the epidemiological evidence. The results show that 29.6% of DALYs would be neglected if the burden caused by the infectivity was not considered. The results also show that DOTS strategy may reduce 97.3% of the number of potential cases infected, 92.9% of DALYs related to TB patients themselves, and 99.9% of DALYs caused by TB's infectivity as well.
文摘The crystal structure of the title compound C16H18NO2+·C7H7SO3-·2H2O, (C23H29NSO7, Mr=463.53) has been determined by single-crystal X-ray diffraction analysis. The crystal belongs to the monoclinic system with space group P21/n, a=8.101(1), b=8.958(2), c=33.281(5)?,β= 94.910(1)(, V=2406.3(7)?3, Z=4, Dc=1.279g/cm3, μ=0.176mm-1, F(000)=984, final R=0.0409, and Rw=0.0860 for 4401 independent reflections. The result shows that in the crystal structure of the title compound the planar cations have two configurations with equal occupation ratio and are antiparally packed through π…π interactions. Similar packing energies in A and B are probably the main factor that leads to the disorder structure.
文摘AIM: To ascertain the adequacy of the microsatellite instability (MSI) as a prognostic indicator by assessing MSI status of patients with double primary gastric and colorectal cancer (DPGCC).METHODS: Sixteen patients were studied, all of whom exhibited sporadic DPGCC, and had no family history of hereditary non-polyposis colorectal cancer, according to the Amsterdam criteria. A total of 32 cancers from 16DPGCC patients, and 216 single primary CRC, were assessed for MSI in 5 microsatellite loci, BAT25, BAT26,D2S123, D5S346, and D17S250.RESULTS: MSI was observed in 6 (37.5%) of 16 GC and 4 (25.0%) of 16 CRC. Thirty tumors (13.9%) out of 216single primary CRC and one tumor (16.7%) out of 6 double primary CRC were found to be microsatellite unstable. Of the 6 GC with MSI in DPGCC, 5 (31.3%) were MSI-high and one (6.3%) was MSI-low. In 5 of 16 DPGCC patients,the cancer recurred in or adjacent to the anastomosis or metastasized to the kidney or lung. The MSI-high DPGCC cases were associated with a younger age of onset (47.5 years vs 62.5 years), higher frequency of lymph node metastasis (100% vs 25%), and advanced Dukes stage (C, 100% vs 41.7%), as well as a higher frequency of recurrence or metastasis (100% vs 8.3%). Only recurrence or metastasis showed statistical significance by Fisher's exact test.CONCLUSION: Our data suggest that MSI may play an important role in the development of DPGCC, and that it may be used clinically as a molecular predictive marker for recurrence or late metastasis of DPGCC.
