Two new C-glucoside flavonoids, namely 8-C-b-D-(2-O-acetyl) glucofuranosyl apigenin and 3-O-acetylvitexin, were isolated from leaves of Crataegus pinnatifida Bge. var. major N. E. Br.. Their structures were elucidated...Two new C-glucoside flavonoids, namely 8-C-b-D-(2-O-acetyl) glucofuranosyl apigenin and 3-O-acetylvitexin, were isolated from leaves of Crataegus pinnatifida Bge. var. major N. E. Br.. Their structures were elucidated by the spectroscopic means and chemical evidence.展开更多
A new labdane-type diterpene glycoside 1 , chlorophytoside A, had been isolated from Chlorophytum laxum R.Br. The structure had been elucidated as (10S)-6α-hydroxy- labda-8,13-dien-15,16-olide 3R-O-β-D-glucopyranosi...A new labdane-type diterpene glycoside 1 , chlorophytoside A, had been isolated from Chlorophytum laxum R.Br. The structure had been elucidated as (10S)-6α-hydroxy- labda-8,13-dien-15,16-olide 3R-O-β-D-glucopyranoside on the basis of chemical and spectroscopic data.展开更多
A new furostanoside, aspafilioside D (1) has been isolated from the root of Asparagus filicinus. Its structure was determined by spectral and chemical methods.
Three new dammarane-type glycosides (1-3) had been isolated from the aerial part of Gynostemma pentaphyllum (Thunb) Makino. The structures of compounds 1-3 had been elucidated as 3b, 12b, 25-trihydroxy-20(S), 24(S)-ep...Three new dammarane-type glycosides (1-3) had been isolated from the aerial part of Gynostemma pentaphyllum (Thunb) Makino. The structures of compounds 1-3 had been elucidated as 3b, 12b, 25-trihydroxy-20(S), 24(S)-epoxydammarane 3-O-b-D-glucopyranosyl (12)-b-D- xylo -pyranoside, 3b, 12b, 25-trihydroxy-20(S), 24(R)-epoxydammarane 3-O-b-D-glucopyranosyl (12) -b-D-xylopyranoside and 3b, 12b, 23b, 25-tetrahydroxy-20(S), 24(S)-epoxydammarane 3-O-b-D- glucopyranosyl (12)-b-D-xylopyranoside, respectively, on the basis of chemical and spectro -scopic methods.展开更多
Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of E...Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of ERK1/2 in human cervical cancer cells, HeLa, by shikonin. Methods The inhibitory effect of shikonin on the growth of HeLa cells was measured by MTT assay. Fluorescent microscopic analysis of apoptotic cells stained with 4’,6’-oliiamiclino-2-phenylindole C (DAPI) and Hoechst 33258 was carried out. Caspase-3 and -8 activities were detected using caspase-3 substrate and caspase-8 substrate as substrates, respectively. The protein levels of ERK, p53 and p-ERK were determined by Western blot analysis.Results Shikonin inhibited cell growth in a time- and dose-dependent manner. Caspase-3 and caspase-8 were activated in the apoptotic process and caspase inhibitors effectively reversed shikonin-induced apoptosis. Phosphorylation of ERK resulted in up-regulation of p53 expression, which was blocked by mitogen-activated protein kinase (MEK), inhibitor PD 98059.Conclusion Shikonin induces HeLa cell apoptosis through the ERK, p53 and caspase pathways.展开更多
文摘Two new C-glucoside flavonoids, namely 8-C-b-D-(2-O-acetyl) glucofuranosyl apigenin and 3-O-acetylvitexin, were isolated from leaves of Crataegus pinnatifida Bge. var. major N. E. Br.. Their structures were elucidated by the spectroscopic means and chemical evidence.
文摘A new labdane-type diterpene glycoside 1 , chlorophytoside A, had been isolated from Chlorophytum laxum R.Br. The structure had been elucidated as (10S)-6α-hydroxy- labda-8,13-dien-15,16-olide 3R-O-β-D-glucopyranoside on the basis of chemical and spectroscopic data.
基金This work was supported by the National Natural Science Fundation of China (30100229) and Science and Technology Development Fundation of Shanghai China (01QB14051) and these supports are gratefully acknowledged.
文摘A new furostanoside, aspafilioside D (1) has been isolated from the root of Asparagus filicinus. Its structure was determined by spectral and chemical methods.
文摘Three new dammarane-type glycosides (1-3) had been isolated from the aerial part of Gynostemma pentaphyllum (Thunb) Makino. The structures of compounds 1-3 had been elucidated as 3b, 12b, 25-trihydroxy-20(S), 24(S)-epoxydammarane 3-O-b-D-glucopyranosyl (12)-b-D- xylo -pyranoside, 3b, 12b, 25-trihydroxy-20(S), 24(R)-epoxydammarane 3-O-b-D-glucopyranosyl (12) -b-D-xylopyranoside and 3b, 12b, 23b, 25-tetrahydroxy-20(S), 24(S)-epoxydammarane 3-O-b-D- glucopyranosyl (12)-b-D-xylopyranoside, respectively, on the basis of chemical and spectro -scopic methods.
文摘Background The role of extracellular signal-regulated kinase 1/2 (ERK1/2) in shikonin-induced HeLa cells apoptosis remains vague. This study was to investigate the activation of caspase pathways and the role of ERK1/2 in human cervical cancer cells, HeLa, by shikonin. Methods The inhibitory effect of shikonin on the growth of HeLa cells was measured by MTT assay. Fluorescent microscopic analysis of apoptotic cells stained with 4’,6’-oliiamiclino-2-phenylindole C (DAPI) and Hoechst 33258 was carried out. Caspase-3 and -8 activities were detected using caspase-3 substrate and caspase-8 substrate as substrates, respectively. The protein levels of ERK, p53 and p-ERK were determined by Western blot analysis.Results Shikonin inhibited cell growth in a time- and dose-dependent manner. Caspase-3 and caspase-8 were activated in the apoptotic process and caspase inhibitors effectively reversed shikonin-induced apoptosis. Phosphorylation of ERK resulted in up-regulation of p53 expression, which was blocked by mitogen-activated protein kinase (MEK), inhibitor PD 98059.Conclusion Shikonin induces HeLa cell apoptosis through the ERK, p53 and caspase pathways.
