AIM:To evaluate the therapeutic effect of compoundChinese drugs, Jianpiyiwei capsule (JPYW) on gastricprecancerous lesions in rats and to explore itsmechanism of action.METHODS:Model of gastric precancerous lesions wa...AIM:To evaluate the therapeutic effect of compoundChinese drugs, Jianpiyiwei capsule (JPYW) on gastricprecancerous lesions in rats and to explore itsmechanism of action.METHODS:Model of gastric precancerous lesions wasconstructed in male Wistar rats: a metal spring wasinserted and fixed through pyloric sphincter. One weekafter recovery, each rat was given 50-60 ℃ hot pastecontainingt50 g/L NaCl 2 mL orally, twice a week for15 weeks.Then 10 normal and 11 model rats wereanaesthetized, after the measurement of gastricmucosa blood flow (GMBF), the rats were killed andthe mucosal hexosamines and malonic dialdehyde(MDA) were measured. The morphological changes ofgastric mucosa were observed macroscopically andmicroscopically, and by an automatic imaging analysissystem. Other rats were treated with JPYW 1.5 g/kg.d-1or 4.5 g/kg@d-1, or distilled water as negative controlrespectively (n=-10 in each group). After 12 weeks, allthe rats were examined as above.RESULTS: The gastric mucosa of model rats showedchronic atrophic gastritis with dysplasia and intestinalmetaplasia (IM), GMBF and hexosamine content werereduced significantly and MDA was increased ascompared to the normal group (P＜0.01). After 12 weekstreatment, the pathological changes of the negativecontrol group became worsened, while in JPYW treatedgroups the changes were modified with significantincrease of GMBF and reduction of MDA, although thehexosamine concentration increased only mildly.CONCLUSION: JPYW increases GMBF and reduces MDAcontent in gastric mucosa and has therapeutic effectson gastric precancerous lesions.展开更多
文摘AIM:To evaluate the therapeutic effect of compoundChinese drugs, Jianpiyiwei capsule (JPYW) on gastricprecancerous lesions in rats and to explore itsmechanism of action.METHODS:Model of gastric precancerous lesions wasconstructed in male Wistar rats: a metal spring wasinserted and fixed through pyloric sphincter. One weekafter recovery, each rat was given 50-60 ℃ hot pastecontainingt50 g/L NaCl 2 mL orally, twice a week for15 weeks.Then 10 normal and 11 model rats wereanaesthetized, after the measurement of gastricmucosa blood flow (GMBF), the rats were killed andthe mucosal hexosamines and malonic dialdehyde(MDA) were measured. The morphological changes ofgastric mucosa were observed macroscopically andmicroscopically, and by an automatic imaging analysissystem. Other rats were treated with JPYW 1.5 g/kg.d-1or 4.5 g/kg@d-1, or distilled water as negative controlrespectively (n=-10 in each group). After 12 weeks, allthe rats were examined as above.RESULTS: The gastric mucosa of model rats showedchronic atrophic gastritis with dysplasia and intestinalmetaplasia (IM), GMBF and hexosamine content werereduced significantly and MDA was increased ascompared to the normal group (P＜0.01). After 12 weekstreatment, the pathological changes of the negativecontrol group became worsened, while in JPYW treatedgroups the changes were modified with significantincrease of GMBF and reduction of MDA, although thehexosamine concentration increased only mildly.CONCLUSION: JPYW increases GMBF and reduces MDAcontent in gastric mucosa and has therapeutic effectson gastric precancerous lesions.
