Purpose:To study the densities and distribution of retinal ganglion cells(RGC)in adult rat retinae with flurogold(FG)labeling retogradely.Methods:FG was injected to the superior colliculi(SC)and dorsal lateral genicul...Purpose:To study the densities and distribution of retinal ganglion cells(RGC)in adult rat retinae with flurogold(FG)labeling retogradely.Methods:FG was injected to the superior colliculi(SC)and dorsal lateral geniculate nuclei(dLGN)in adult rats and the retinae were examined by fluorescence microscopy at various periods of time.Results;FG-labelled RGC were observed in the retina as early as 3days after application of FG.The labelled cells gradually increased in density,reached95%of the maximal number on days7and the maximal number on days30.The density of labelled cells was higher in the posterior pole than in the peripheral area.The fluorescence intensity in labelled cells maintained up to 60days.Conclusion:The FG retrograde labeling method is reliable and effective for quantity of RGC.Eye Science2000;16:29-33.展开更多
基金The project was supported by the National Natural Science Foundation of China (No.39900076)
文摘Purpose:To study the densities and distribution of retinal ganglion cells(RGC)in adult rat retinae with flurogold(FG)labeling retogradely.Methods:FG was injected to the superior colliculi(SC)and dorsal lateral geniculate nuclei(dLGN)in adult rats and the retinae were examined by fluorescence microscopy at various periods of time.Results;FG-labelled RGC were observed in the retina as early as 3days after application of FG.The labelled cells gradually increased in density,reached95%of the maximal number on days7and the maximal number on days30.The density of labelled cells was higher in the posterior pole than in the peripheral area.The fluorescence intensity in labelled cells maintained up to 60days.Conclusion:The FG retrograde labeling method is reliable and effective for quantity of RGC.Eye Science2000;16:29-33.
