AIM:To investigate the effect of transfected survivin antisense oligonucleotide (ASODN) on proliferation and apoptosis of gastric cancer ceils. METHODS:The authors designed ASODNs targeting different regions of survMn...AIM:To investigate the effect of transfected survivin antisense oligonucleotide (ASODN) on proliferation and apoptosis of gastric cancer ceils. METHODS:The authors designed ASODNs targeting different regions of survMn mRNA,including surviving ASODN1,ASODN2 and ASODN3.ASODNs were transfected into gastric cancer cell line SGC 7901,cell growth was detected by MTT assay.Cells exposed to the potent oligonucleotide were also examined for apoptosis induction by FCM and fluorescence microscopy.Semiquantitive RT-PCR and Western blot examinations were carried for expression of survivin mRNA and protein. RESULTS:ASODN3 caused a statistically significant reduction of cell viability to 60.6% (±2.9%) (P<0.01),while ASODN1 and ASODN2 had no such changes (P>0.05).The cell growth was also significantly inhibited by ASODN3, compared with reversal and scrambled sequence.A significant loss of survivin mRNA was presented in ASODN3 treated ceils and this was not seen in treatment with sense ODN or scramble ODN.Protein level was significantly decreased 48 h after survivin ASODN trasfected by approximately 2-fold decrease comparedwith untreated controls.However. ASODN3 did not induce significant apoptosis response until 48 h after transfection (P>0.05). CONCLUSION:ASODN3,which targets translation initiation part,can be identified as a most potent antisense compound. Srvivin ASODN3 may provide a novel approach to therapy of gastric cancer.展开更多
AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied b...AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied by using FT-IR and thespecial spectra characteristics were analyzed in differenttissues.RESULTS: Different spectra were found in normal andcancerous tissues. The peak at 1 550/cm was weak andwide in cancerous tissues but strong and high in normaltissues.The ratio of I1 647/I1 550 was 2.0 in normal tissuesand 2.36 in cancerous tissues (P＜0.05). The ratio of Ⅰ1 550/I 1 080 was 4.5 in normal tissues and 3.4 in canceroustissues (P＜0.01). The peak at 1453/cm was higher than at1 402/cm in normal tissue and lower than at 1 402/cm incancerous tissues.CONCLUSION: The results indicate that F-FIR may be used in clinical diagnosis.展开更多
AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (...AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology. METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies. RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research. CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopie method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.展开更多
基金Supported by Shanghai Key Research Project Grant in Medical Science Development (99ZD003)
文摘AIM:To investigate the effect of transfected survivin antisense oligonucleotide (ASODN) on proliferation and apoptosis of gastric cancer ceils. METHODS:The authors designed ASODNs targeting different regions of survMn mRNA,including surviving ASODN1,ASODN2 and ASODN3.ASODNs were transfected into gastric cancer cell line SGC 7901,cell growth was detected by MTT assay.Cells exposed to the potent oligonucleotide were also examined for apoptosis induction by FCM and fluorescence microscopy.Semiquantitive RT-PCR and Western blot examinations were carried for expression of survivin mRNA and protein. RESULTS:ASODN3 caused a statistically significant reduction of cell viability to 60.6% (±2.9%) (P<0.01),while ASODN1 and ASODN2 had no such changes (P>0.05).The cell growth was also significantly inhibited by ASODN3, compared with reversal and scrambled sequence.A significant loss of survivin mRNA was presented in ASODN3 treated ceils and this was not seen in treatment with sense ODN or scramble ODN.Protein level was significantly decreased 48 h after survivin ASODN trasfected by approximately 2-fold decrease comparedwith untreated controls.However. ASODN3 did not induce significant apoptosis response until 48 h after transfection (P>0.05). CONCLUSION:ASODN3,which targets translation initiation part,can be identified as a most potent antisense compound. Srvivin ASODN3 may provide a novel approach to therapy of gastric cancer.
基金the National Natural Science Foundation of China, No.39730160
文摘AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied by using FT-IR and thespecial spectra characteristics were analyzed in differenttissues.RESULTS: Different spectra were found in normal andcancerous tissues. The peak at 1 550/cm was weak andwide in cancerous tissues but strong and high in normaltissues.The ratio of I1 647/I1 550 was 2.0 in normal tissuesand 2.36 in cancerous tissues (P＜0.05). The ratio of Ⅰ1 550/I 1 080 was 4.5 in normal tissues and 3.4 in canceroustissues (P＜0.01). The peak at 1453/cm was higher than at1 402/cm in normal tissue and lower than at 1 402/cm incancerous tissues.CONCLUSION: The results indicate that F-FIR may be used in clinical diagnosis.
基金Supported by the National Natural Science Foundation of China, No. 30371604 State Key Project of China, No. 2002CCA01900
文摘AIM: Real-time and rapid Identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology. METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies. RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research. CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopie method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.
