Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into thre...Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups After 30day experiment, myocardial histopathological observation was performed. Location and distribution of NFκB p50 was examined by immunohistochemical assay. Expression of NFκB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NFκB; Myocardium p53 gene expression was examined by RTPCR analysis Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group Compared with control group, there were many myocardium nucleuses, which expressed NFκB p50 and distribute under epicardium Expression of NFκB p50 protein in nucleus increased significantly in ADR group The NFκB binding activity increased significantly in ADR group Myocardium expressions of p53 mRNA increased in ADR group Conclusions The NFκB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NFκB plays an important role in causing degeneration of myocardial tissue and regulating expression of relatedapoptosis genes展开更多
Objective To review the recent development of functional MRI application in epilepsy. Data sources Both Chinese and English language literatures were researched using MEDLINE/ CD ROM (1996-2005) and the Chinese Biom...Objective To review the recent development of functional MRI application in epilepsy. Data sources Both Chinese and English language literatures were researched using MEDLINE/ CD ROM (1996-2005) and the Chinese Biomedical Literature Disk (1996-2005). Study selection Published articles about functional MRI application and epilepsy were selected.Data extraction Data were mainly extracted from 38 articles which are listed in the reference section of this review.Results fMRI can be used to localize seizure foci through detecting these cerebral hemodynamic changes produced by epileptiform discharges. EEG-triggered fMRI, which has higher spatial and temporal resolution, helps to detect the spatiotemporal pattern of spike origin and propagation, and define localization of the epileptogenic focus. fMRI is also useful in language and memory cognitive function assessment and presurgical assessment of refractory epilepsy. Atypically distributed cognitive function areas can be detected by fMRI, because of cortical language and memory areas reorganization during long-term epileptic activity in patients with epilepsy. Conclusions fMRI technique plays a very important role in cognitive function and presurgical assessment of patients with epilepsy. It is meaningful for understanding pathogenesis of epilepsy.展开更多
文摘Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups After 30day experiment, myocardial histopathological observation was performed. Location and distribution of NFκB p50 was examined by immunohistochemical assay. Expression of NFκB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NFκB; Myocardium p53 gene expression was examined by RTPCR analysis Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group Compared with control group, there were many myocardium nucleuses, which expressed NFκB p50 and distribute under epicardium Expression of NFκB p50 protein in nucleus increased significantly in ADR group The NFκB binding activity increased significantly in ADR group Myocardium expressions of p53 mRNA increased in ADR group Conclusions The NFκB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NFκB plays an important role in causing degeneration of myocardial tissue and regulating expression of relatedapoptosis genes
文摘Objective To review the recent development of functional MRI application in epilepsy. Data sources Both Chinese and English language literatures were researched using MEDLINE/ CD ROM (1996-2005) and the Chinese Biomedical Literature Disk (1996-2005). Study selection Published articles about functional MRI application and epilepsy were selected.Data extraction Data were mainly extracted from 38 articles which are listed in the reference section of this review.Results fMRI can be used to localize seizure foci through detecting these cerebral hemodynamic changes produced by epileptiform discharges. EEG-triggered fMRI, which has higher spatial and temporal resolution, helps to detect the spatiotemporal pattern of spike origin and propagation, and define localization of the epileptogenic focus. fMRI is also useful in language and memory cognitive function assessment and presurgical assessment of refractory epilepsy. Atypically distributed cognitive function areas can be detected by fMRI, because of cortical language and memory areas reorganization during long-term epileptic activity in patients with epilepsy. Conclusions fMRI technique plays a very important role in cognitive function and presurgical assessment of patients with epilepsy. It is meaningful for understanding pathogenesis of epilepsy.
