With a data set of Eurasian forest litter fall based on 471 stands,annual litterfall was estimated to be 6.53Pg dm·a^-1 (1Pg=10^15g;dm,dry matter)in Eurasian forests,of which more than half occurred in tropical a...With a data set of Eurasian forest litter fall based on 471 stands,annual litterfall was estimated to be 6.53Pg dm·a^-1 (1Pg=10^15g;dm,dry matter)in Eurasian forests,of which more than half occurred in tropical and subtropical forests,and a third in the boreal area.With litterfall,around 2.94Pg C per year is transferred from the forest vegetation to the soil on this continent.展开更多
To evaluate the biocide effect of quaternary ammonium chloride (QAC), a whitewater sample was taken from a fine papermachine headbox. By plate spreading method, 51 strains of facultative anaerobe were isolated morphol...To evaluate the biocide effect of quaternary ammonium chloride (QAC), a whitewater sample was taken from a fine papermachine headbox. By plate spreading method, 51 strains of facultative anaerobe were isolated morphologically. Then the strains were separately transferred to basal medium and were incubated before the beginning of log phase. To identify strains with different QAC resistance, 30-120 ppmof N-Alkyl-benzyl-dimethyl ammonium chloride were added to basal medium.Biocide effect was investigated by comparison of bacterial growth, which can be monitored by 600 nm light absorbance of basal medium suspension. Among 51 strains, only 2 strains can Survive for QAC concentration up to 120 ppm. API20E aud 16S rRNA gene sequencing technique were applied to identify two strains with highest (120 ppm)QAC resistance. One strain (HB22) was identified as Morganella morganii. HB22 can resist QAC concentration up to 150 ppm. HB22 isGram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole positive, H2S production negative, facultative anaerobe. HB22 has optimum growth condition of 35℃ and pH 7.0.HB22 can catabolize only glucose and D(+)-Mannose. The other (HB45) was identified with high similarity among Pseudomonas cf. monteili or Pseudomonas mosselii or Pseudomonas putida. HB45 can resist QAC concentration up to 200 ppm. HB45 is Gram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole negative, H2S production negative, aerobe. HB45 has optimum growth condition of 30℃ and pH 7.4. HB45 can catabolize L-Arabinose, L(+)-Rhanmose, D(+)-Mannose, Glucose, Glycerol, Lactose, Maltose, Raffmose, Xylose, Cellulose and Xylan. The implication of this work to paper industry is also discussed.展开更多
To obtain a primary overview of gene diversity and expression pattern inLycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were se-quenced and4,687 cleaned ESTs were used for gene expression...To obtain a primary overview of gene diversity and expression pattern inLycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were se-quenced and4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets(totally 1,066 ESTs) had homologues in GenBank and 3,621 ESTs were novel.展开更多
文摘With a data set of Eurasian forest litter fall based on 471 stands,annual litterfall was estimated to be 6.53Pg dm·a^-1 (1Pg=10^15g;dm,dry matter)in Eurasian forests,of which more than half occurred in tropical and subtropical forests,and a third in the boreal area.With litterfall,around 2.94Pg C per year is transferred from the forest vegetation to the soil on this continent.
文摘To evaluate the biocide effect of quaternary ammonium chloride (QAC), a whitewater sample was taken from a fine papermachine headbox. By plate spreading method, 51 strains of facultative anaerobe were isolated morphologically. Then the strains were separately transferred to basal medium and were incubated before the beginning of log phase. To identify strains with different QAC resistance, 30-120 ppmof N-Alkyl-benzyl-dimethyl ammonium chloride were added to basal medium.Biocide effect was investigated by comparison of bacterial growth, which can be monitored by 600 nm light absorbance of basal medium suspension. Among 51 strains, only 2 strains can Survive for QAC concentration up to 120 ppm. API20E aud 16S rRNA gene sequencing technique were applied to identify two strains with highest (120 ppm)QAC resistance. One strain (HB22) was identified as Morganella morganii. HB22 can resist QAC concentration up to 150 ppm. HB22 isGram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole positive, H2S production negative, facultative anaerobe. HB22 has optimum growth condition of 35℃ and pH 7.0.HB22 can catabolize only glucose and D(+)-Mannose. The other (HB45) was identified with high similarity among Pseudomonas cf. monteili or Pseudomonas mosselii or Pseudomonas putida. HB45 can resist QAC concentration up to 200 ppm. HB45 is Gram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole negative, H2S production negative, aerobe. HB45 has optimum growth condition of 30℃ and pH 7.4. HB45 can catabolize L-Arabinose, L(+)-Rhanmose, D(+)-Mannose, Glucose, Glycerol, Lactose, Maltose, Raffmose, Xylose, Cellulose and Xylan. The implication of this work to paper industry is also discussed.
基金Hi-tech Research &Development Program of China (863 Program, No. 2002AA241051) and Science & Technology Program for Agriculture Development of Shanghai.
文摘To obtain a primary overview of gene diversity and expression pattern inLycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were se-quenced and4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets(totally 1,066 ESTs) had homologues in GenBank and 3,621 ESTs were novel.
