Chronic treatment with Salvia Miltiorrhiza preventing left ventricular hypertrophy (L VH) and its possible mechanism- inhibiting the action of cardiac aldosterone in spontaneously hypertensive rats (SHR) were invest...Chronic treatment with Salvia Miltiorrhiza preventing left ventricular hypertrophy (L VH) and its possible mechanism- inhibiting the action of cardiac aldosterone in spontaneously hypertensive rats (SHR) were investigated.Normotensive Wistar- kyoto (WKY ) rats and SHRs were used.Part of SHRs was treated with Salvia Miltiorrhiza for 12 weeks.Systolic blood pres- sure (SBP) and left ventricular mass index were measured.Sections of heart tissue were stained with HE method and Van Gieson method.Collagen volume fraction was determined in the leftven- tricle by automatically quantitative m orphometry.Cardiac aldosterone concentration was measured by radioimm unoassay.The results indicated thatcom pared with WKY rats,SHRs exhibited high- er SBP,left ventricular collagen volume fraction,and aldosterone concentration (all P<0 .0 5 ) . After the treatm ent with Salvia Miltiorrhiza,SBP,left ventricular collagen volum e fraction,and aldosterone concentration in SHR were decreased as compared with control group (P<0 .0 5 ) ex- cept SBP.It was concluded thatchronic treatment with Salvia Miltiorrhiza could preventleftven- tricular hypertrophy in SHR,significantly inhibit collagen compositions in left ventricle.The m echanism was probably related with the inhibition of the cardiac aldosterone action.展开更多
The heart is an organ frequently affected in the acquired immunodeficiency syndrome.~1 But there is little information as to whether the coronary artery calcification can identify asymptomatic individuals at high risk...The heart is an organ frequently affected in the acquired immunodeficiency syndrome.~1 But there is little information as to whether the coronary artery calcification can identify asymptomatic individuals at high risk for having cardiac morphological and functional abnormalities and cardiac risk factors in human immunodeficiency virus (HIV)-infected persons. Accordingly, the purpose of this study was to determine whether coronary artery calcification was associated with cardiac morphological and functional abnormalities, atherogenic lipid and C-reactive protein (CRP) changes in a black adult population with HIV-1 infection.展开更多
Background:Tolerogenic dendritic cells(DCs)are associated with poor prognosis of sepsis.Matrix metalloproteinases(MMPs)have been shown to have immunomodulatory effects.However,whether MMPs are involved in the function...Background:Tolerogenic dendritic cells(DCs)are associated with poor prognosis of sepsis.Matrix metalloproteinases(MMPs)have been shown to have immunomodulatory effects.However,whether MMPs are involved in the functional reprogramming of DCs is unknown.The study aims to investigate the role of MMPs in sepsis-induced DCs tolerance and the potential mechanisms.Methods:A murine model of late sepsis was induced by cecal ligation and puncture(CLP).The expression levels of members of the MMP family were detected in sepsis-induced tolerogenic DCs by using microarray assessment.The potential roles and mechanisms underlying MMP8 in the differentiation,maturation and functional reprogramming of DCs during late sepsis were assessed both in vitro and in vivo.Results:DCs from late septic mice expressed higher levels of MMP8,MMP9,MMP14,MMP19,MMP25 and MMP27,and MMP8 levels were the highest.MMP8 deficiency significantly alleviated sepsis-induced immune tolerance of DCs both in vivo and in vitro.Adoptive transfer of MMP8 knockdown post-septic bone marrow-derived DCs protected mice against sepsis-associated lethality and organ dysfunction,inhibited regulatory T-cell expansion and enhanced Th1 response.Furthermore,the effect of MMP8 on DC tolerance was found to be associated with the nuclear factor kappa-B p65/β-catenin pathway.Conclusions:Increased MMP8 levels in septic DCs might serve as a negative feedback loop,thereby suppressing the proinflammatory response and inducing DC tolerance.展开更多
基金Thisprojectwassupported by a grant from Natural Sci-ences Foundation of Hubei Province(No. 2 0 0 0 J0 6 4 )
文摘Chronic treatment with Salvia Miltiorrhiza preventing left ventricular hypertrophy (L VH) and its possible mechanism- inhibiting the action of cardiac aldosterone in spontaneously hypertensive rats (SHR) were investigated.Normotensive Wistar- kyoto (WKY ) rats and SHRs were used.Part of SHRs was treated with Salvia Miltiorrhiza for 12 weeks.Systolic blood pres- sure (SBP) and left ventricular mass index were measured.Sections of heart tissue were stained with HE method and Van Gieson method.Collagen volume fraction was determined in the leftven- tricle by automatically quantitative m orphometry.Cardiac aldosterone concentration was measured by radioimm unoassay.The results indicated thatcom pared with WKY rats,SHRs exhibited high- er SBP,left ventricular collagen volume fraction,and aldosterone concentration (all P<0 .0 5 ) . After the treatm ent with Salvia Miltiorrhiza,SBP,left ventricular collagen volum e fraction,and aldosterone concentration in SHR were decreased as compared with control group (P<0 .0 5 ) ex- cept SBP.It was concluded thatchronic treatment with Salvia Miltiorrhiza could preventleftven- tricular hypertrophy in SHR,significantly inhibit collagen compositions in left ventricle.The m echanism was probably related with the inhibition of the cardiac aldosterone action.
文摘The heart is an organ frequently affected in the acquired immunodeficiency syndrome.~1 But there is little information as to whether the coronary artery calcification can identify asymptomatic individuals at high risk for having cardiac morphological and functional abnormalities and cardiac risk factors in human immunodeficiency virus (HIV)-infected persons. Accordingly, the purpose of this study was to determine whether coronary artery calcification was associated with cardiac morphological and functional abnormalities, atherogenic lipid and C-reactive protein (CRP) changes in a black adult population with HIV-1 infection.
基金supported partly by grants from the National Natural Science Foundation of China(81871583)Key R&D Program Projects of Zhejiang Province(2021C03072)the Natural Science Foundation of Zhejiang Province(LQ19H100003).
文摘Background:Tolerogenic dendritic cells(DCs)are associated with poor prognosis of sepsis.Matrix metalloproteinases(MMPs)have been shown to have immunomodulatory effects.However,whether MMPs are involved in the functional reprogramming of DCs is unknown.The study aims to investigate the role of MMPs in sepsis-induced DCs tolerance and the potential mechanisms.Methods:A murine model of late sepsis was induced by cecal ligation and puncture(CLP).The expression levels of members of the MMP family were detected in sepsis-induced tolerogenic DCs by using microarray assessment.The potential roles and mechanisms underlying MMP8 in the differentiation,maturation and functional reprogramming of DCs during late sepsis were assessed both in vitro and in vivo.Results:DCs from late septic mice expressed higher levels of MMP8,MMP9,MMP14,MMP19,MMP25 and MMP27,and MMP8 levels were the highest.MMP8 deficiency significantly alleviated sepsis-induced immune tolerance of DCs both in vivo and in vitro.Adoptive transfer of MMP8 knockdown post-septic bone marrow-derived DCs protected mice against sepsis-associated lethality and organ dysfunction,inhibited regulatory T-cell expansion and enhanced Th1 response.Furthermore,the effect of MMP8 on DC tolerance was found to be associated with the nuclear factor kappa-B p65/β-catenin pathway.Conclusions:Increased MMP8 levels in septic DCs might serve as a negative feedback loop,thereby suppressing the proinflammatory response and inducing DC tolerance.
