AIM: To construct and identify the recombinant vectors carrying herpes simplex virus thymidine kinase (HSV-TK) and tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) genes expressed in gastric carcinoma cell...AIM: To construct and identify the recombinant vectors carrying herpes simplex virus thymidine kinase (HSV-TK) and tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) genes expressed in gastric carcinoma cell line SGC7901. METHODS: The fragments of HSV-TK, internal ribosome entry sites (IRES) and TNF-α or IL-2 genes were inserted in a TK-IRES-TNF-α or TK-IRES-IL-2 order into pEGFP-N3 and pLXSN to generate the therapeutic vectors pEGFP-TT,pEGFP-TI, pL(TT)SN and pL(TI)SN respectively, which were structurally confirmed by the digestion analysis of restriction endonuclease. The former two plasmids were used for the transient expression of recombinant proteins in the target cells while pL(TT)SN and pL(TI)SN were transfected into SGC7901 cells by lipofectamine for the stable expression of objective genes through G418 selection. The protein products expressed transiently and stably in SGC7901 cells by the constructed vectors were confirmed by fluorescent microscopy and Western blot respectively. RESULTS: The inserted fragme.nts in all constructed plasmids were structurally confirmed to be consistent with that of the published data. In the transient expression, both pEGFP-TT and pEGFP-TI were shown expressed in nearly 50% of the transfected SGC7901 cells. Similarly, the G418 selected vectors PL(TT)SN and PL(TI)SN were confirmed to be successful in the stable expression of the objective proteins in the target cells. CONCLUSION: The constructed recombinant vectors in the present study that can express the suicide gene TK in combination with cytokines genes may serve as the potential tools to perform more effective investigations in future for the gene therapy of gastric carcinoma.展开更多
AIM: To evaluate the synergistic antitumor effects of herpes simplex virus thymidine kinase (HSV-TK) together with tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) gene expression on gastric cancer cell li...AIM: To evaluate the synergistic antitumor effects of herpes simplex virus thymidine kinase (HSV-TK) together with tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) gene expression on gastric cancer cell line SGC7901. METHODS: Recombinant vectors pL(TT)SN and pL(TI)SN,which express TK-IRES-TNF-α and TK-IRES-IL-2 genes separately, as well as the control plasmids pL(TK)SN and pLXSN were employed to transfect PA317 cells respectively to generate the viruses that can stably express the objective genes through G418 selection. The gastric cancer cells were then transfected by the retroviral serum from the package cells and maintained in culture to determine the cell growth and apoptosis. The cytotoxic effects of HSV-TK together with TNF-α or IL-2 gene expression on the transfected cancer cells were evaluated by the cell viability and bystander effects in the presence of GCV supplemented in the cultural medium. RESULTS: Expression of recombinant proteins including TNF-α and IL-2 by stable transfectants was confirmed by Western blotting. The percentage of cell apoptosis in the SGC/0, SGC/TK-TNF-α SGC/TK-IL-2 and SGC/TK done was 2.3%, 12.3%, 11.1% and 10.9% respectively at 24 h posttransfection. Cell growth status among all the experimental groups as judged by cell absorbance (A) at 570nm did not exhibit any significant difference (P>0.05); although it was noted to be slightly lower in the SGC/TT group. Cell survival rate in SGC/TI, SGC/TT and SGC/TK group was significantly decreased in a dose-dependent manner of GCV compared with that of the SGC/0 group (P<0.05-0.01). Among all studied cells, the SGC/TT was shown most sensitive to GCV with a half lethal dose of 0.5 mg.L^-1. In contrast, the survival rate of SGC/0 cells was not affected by the presence of GCV with the doses less than 10 mg-L^-1 The half lethal dose of GCV for SGC/0 cells was more than 100 mg-L^-1. Marked bystander effect induced by SGC/TI, SGC/TT and SGC/TK cells was confirmed by the fact that 20% of these stable transfectants could kill 50% of the co-cultured cells, in which the most prominent bystander effect was found in the circumstance of SGC/TT presence. However, no significant difference of these variables was found among SGC/TI,SGC/TT and SGC/TK cells (P>0.05). CONCLUSION: The synergistic antitumor effects produced by the co-expression of HSV-TK with TNF-α or IL-2 geneswere not present in the transfected SGC7901 cells. The mechanism underlying these phenomena was not known.展开更多
Simulating biological olfactory neural system, KⅢnetwork, which is a high-dimensional chaotic neural network, is designed in this paper. Different from conventional artificial neural network, the KⅢnetwork works...Simulating biological olfactory neural system, KⅢnetwork, which is a high-dimensional chaotic neural network, is designed in this paper. Different from conventional artificial neural network, the KⅢnetwork works in its chaotic trajectory. It can simulate not only the output EEG waveform observed in electrophysiological experiments, but also the biological intelligence for pattern classification. The simulation analysis and application to the recognition of handwriting numerals are presented here. The classification performance of the KⅢnetwork at different noise levels was also investigated.展开更多
A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear ...A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear stochastic optimal control to minimize the response of quasi non-integrable Hamiltonian systems subject to Gaussian white noise excitation.By using the stochastic averaging method for quasi non-integrable Hamiltonian systems the equations of motion of a controlled quasi non-integrable Hamiltonian system is reduced to a one-dimensional av- eraged It stochastic differential equation.By using the stochastic dynamical programming princi- ple the dynamical programming equation for minimizing the response of the system is formulated. The optimal control law is derived from the dynamical programming equation and the bounded control constraints.The response of optimally controlled systems is predicted through solving the FPK equation associated with It stochastic differential equation.An example is worked out in detail to illustrate the application of the control strategy proposed.展开更多
AIM The aim of the present study was to explore cytotoxic activity and the mechanism of tumor cell killing by isorhamnetin and to investigate the effect of isorhamnetin on tumor growth, cell prolification and apoptosi...AIM The aim of the present study was to explore cytotoxic activity and the mechanism of tumor cell killing by isorhamnetin and to investigate the effect of isorhamnetin on tumor growth, cell prolification and apoptosis in transplantation tumor of lung cancer of Lewis cell line in C57BL/6 mice. METHODS Human A549 cells were treated with 10-320(g/ml isorhamnetin, C57BL/6 mice were subcutaneously inoculated Lewis cells 0.2ml/each (1×107cells/ml) below the right forelimb armpit and were treated with 50 (g/ml isorhamnetin isorhamnetin.The results were observed and analyzed under light-microscope, electronic microscopy, growth inhibition was analyzed by MTT, clonogenic asssays and growth curve;the apoptosis and the expression-associated genes peaks were detected with flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay,展开更多
The tendency of particles in a water-saturated granular mass to re-arrange into a denser state during cyclic shearing under pressure results in an increase in pore water pressure. The increase in the pore water pressu...The tendency of particles in a water-saturated granular mass to re-arrange into a denser state during cyclic shearing under pressure results in an increase in pore water pressure. The increase in the pore water pressure causes a reduction in the inner particle contact forces, and in turn easier re-arrangement of the particles. Eventually, the material loses its shear strength, partially or almost completely. In this paper, a general three-dimensional continuum mechanics model is presented for the deformation of granular materials. A physically based model is also presented for characterization of liquefaction of the water saturated granular material under undrained cyclic shearing. The model incorporates the fabric of the granular mass, which develops as the frictional granular mass is deformed in shear. It includes the coupling between shearing and excess pore water pressure. The model parameters are estimated, based on the results of cyclic shearing experiments on large hollow cylindrical samples of silica sand. Basically, the calculation results utilizing this model can embody liquefaction phenomena of the water saturated granular material under undrained cyclic shearing.展开更多
In this paper we develop a general exact fan-beam reconstruction algorithm for free-form trajectories not only closed but also unclosed,based on the fan-beam reconstruction formula recently developed by Noo et al.. A ...In this paper we develop a general exact fan-beam reconstruction algorithm for free-form trajectories not only closed but also unclosed,based on the fan-beam reconstruction formula recently developed by Noo et al.. A mathematical proof is then provided with the geometrical explanation of equi-spatial detectors. With this algorithm we can obtain exact region of interest (ROI) reconstruction if and only if every projecting line passing through the ROI intersects the free-form source trajectory,when the projections are not truncated. Furthermore,under the condi- tion that the source-to-detectors distance changes slowly enough relative to the length itself,we obtain a very good approximate reconstruction algorithm,which is the same as the algorithm of the circular trajectory except that the source-to-detectors distance is a function of the rotation angle. Then the algorithms are tested using the Shepp-Logan phantom and the experiment shows that the algorithms can get perfect numerical results.展开更多
Mutual information has currently been one of the most intensivelyresearched measures. It has been proven to be accurate and effective registrationmeasure. Despite the general promising results, mutual information some...Mutual information has currently been one of the most intensivelyresearched measures. It has been proven to be accurate and effective registrationmeasure. Despite the general promising results, mutual information sometimes mightlead to misregistration because of neglecting spatial information and treating intensityvariations with undue sensitivity. In this paper, an extension of mutual informationframework was proposed in which higher-order spatial information regarding imagestructures was incorporated into the registration processing of PET and MR. Thesecond-order estimate of mutual information algorithm was applied to the registrationof seven patients. Evaluation from Vanderbilt University and our visual inspectionshowed that sub-voxel accuracy and robust results were achieved in all cases withsecond-order mutual information as the similarity measure and with Powell's multi-dimensional direction set method as optimization strategy.展开更多
This paper presents a method for unsupervised segmentation of images consisting of multiple textures. The images under study are modeled by a proposed hierarchical random field model, which has two layers. The first l...This paper presents a method for unsupervised segmentation of images consisting of multiple textures. The images under study are modeled by a proposed hierarchical random field model, which has two layers. The first layer is modeled as a Markov Random Field (MRF) representing an unobservable region image and the second layer uses 'Filters, Random and Maximum Entropy (Abb. FRAME)' model to represent multiple textures which cover each region. Compared with the traditional Hierarchical Markov Random Field (HMRF), the FRAME can use a bigger neighborhood system and model more complex patterns. The segmentation problem is formulated as Maximum a Posteriori (MAP) estimation according to the Bayesian rule. The iterated conditional modes (ICM) algorithm is carried out to find the solution of the MAP estimation. An algorithm based on the local entropy rate is proposed to simplify the estimation of the parameters of MRF. The parameters of FRAME are estimated by the ExpectationMaximum (EM) algorithm. Finally, an experiment with synthesized and real images is given, which shows that the method can segment images with complex textures efficiently and is robust to noise.展开更多
基金Supported by Doctoral Foundation of Xi'an Jiaotong University,No.69925101 National Natural Science Foundation of China,No.30270404
文摘AIM: To construct and identify the recombinant vectors carrying herpes simplex virus thymidine kinase (HSV-TK) and tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) genes expressed in gastric carcinoma cell line SGC7901. METHODS: The fragments of HSV-TK, internal ribosome entry sites (IRES) and TNF-α or IL-2 genes were inserted in a TK-IRES-TNF-α or TK-IRES-IL-2 order into pEGFP-N3 and pLXSN to generate the therapeutic vectors pEGFP-TT,pEGFP-TI, pL(TT)SN and pL(TI)SN respectively, which were structurally confirmed by the digestion analysis of restriction endonuclease. The former two plasmids were used for the transient expression of recombinant proteins in the target cells while pL(TT)SN and pL(TI)SN were transfected into SGC7901 cells by lipofectamine for the stable expression of objective genes through G418 selection. The protein products expressed transiently and stably in SGC7901 cells by the constructed vectors were confirmed by fluorescent microscopy and Western blot respectively. RESULTS: The inserted fragme.nts in all constructed plasmids were structurally confirmed to be consistent with that of the published data. In the transient expression, both pEGFP-TT and pEGFP-TI were shown expressed in nearly 50% of the transfected SGC7901 cells. Similarly, the G418 selected vectors PL(TT)SN and PL(TI)SN were confirmed to be successful in the stable expression of the objective proteins in the target cells. CONCLUSION: The constructed recombinant vectors in the present study that can express the suicide gene TK in combination with cytokines genes may serve as the potential tools to perform more effective investigations in future for the gene therapy of gastric carcinoma.
基金Supported by Doctoral Foundation of Xi'an Jiaotong University,No.69925101National Natural Science Foundation of China,No.30270404
文摘AIM: To evaluate the synergistic antitumor effects of herpes simplex virus thymidine kinase (HSV-TK) together with tumor necrosis factor alpha (TNF-α) or interleukin-2 (IL-2) gene expression on gastric cancer cell line SGC7901. METHODS: Recombinant vectors pL(TT)SN and pL(TI)SN,which express TK-IRES-TNF-α and TK-IRES-IL-2 genes separately, as well as the control plasmids pL(TK)SN and pLXSN were employed to transfect PA317 cells respectively to generate the viruses that can stably express the objective genes through G418 selection. The gastric cancer cells were then transfected by the retroviral serum from the package cells and maintained in culture to determine the cell growth and apoptosis. The cytotoxic effects of HSV-TK together with TNF-α or IL-2 gene expression on the transfected cancer cells were evaluated by the cell viability and bystander effects in the presence of GCV supplemented in the cultural medium. RESULTS: Expression of recombinant proteins including TNF-α and IL-2 by stable transfectants was confirmed by Western blotting. The percentage of cell apoptosis in the SGC/0, SGC/TK-TNF-α SGC/TK-IL-2 and SGC/TK done was 2.3%, 12.3%, 11.1% and 10.9% respectively at 24 h posttransfection. Cell growth status among all the experimental groups as judged by cell absorbance (A) at 570nm did not exhibit any significant difference (P>0.05); although it was noted to be slightly lower in the SGC/TT group. Cell survival rate in SGC/TI, SGC/TT and SGC/TK group was significantly decreased in a dose-dependent manner of GCV compared with that of the SGC/0 group (P<0.05-0.01). Among all studied cells, the SGC/TT was shown most sensitive to GCV with a half lethal dose of 0.5 mg.L^-1. In contrast, the survival rate of SGC/0 cells was not affected by the presence of GCV with the doses less than 10 mg-L^-1 The half lethal dose of GCV for SGC/0 cells was more than 100 mg-L^-1. Marked bystander effect induced by SGC/TI, SGC/TT and SGC/TK cells was confirmed by the fact that 20% of these stable transfectants could kill 50% of the co-cultured cells, in which the most prominent bystander effect was found in the circumstance of SGC/TT presence. However, no significant difference of these variables was found among SGC/TI,SGC/TT and SGC/TK cells (P>0.05). CONCLUSION: The synergistic antitumor effects produced by the co-expression of HSV-TK with TNF-α or IL-2 geneswere not present in the transfected SGC7901 cells. The mechanism underlying these phenomena was not known.
文摘Simulating biological olfactory neural system, KⅢnetwork, which is a high-dimensional chaotic neural network, is designed in this paper. Different from conventional artificial neural network, the KⅢnetwork works in its chaotic trajectory. It can simulate not only the output EEG waveform observed in electrophysiological experiments, but also the biological intelligence for pattern classification. The simulation analysis and application to the recognition of handwriting numerals are presented here. The classification performance of the KⅢnetwork at different noise levels was also investigated.
基金Project supported by the National Natural Science Foundation of China(No.19972059).
文摘A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear stochastic optimal control to minimize the response of quasi non-integrable Hamiltonian systems subject to Gaussian white noise excitation.By using the stochastic averaging method for quasi non-integrable Hamiltonian systems the equations of motion of a controlled quasi non-integrable Hamiltonian system is reduced to a one-dimensional av- eraged It stochastic differential equation.By using the stochastic dynamical programming princi- ple the dynamical programming equation for minimizing the response of the system is formulated. The optimal control law is derived from the dynamical programming equation and the bounded control constraints.The response of optimally controlled systems is predicted through solving the FPK equation associated with It stochastic differential equation.An example is worked out in detail to illustrate the application of the control strategy proposed.
文摘AIM The aim of the present study was to explore cytotoxic activity and the mechanism of tumor cell killing by isorhamnetin and to investigate the effect of isorhamnetin on tumor growth, cell prolification and apoptosis in transplantation tumor of lung cancer of Lewis cell line in C57BL/6 mice. METHODS Human A549 cells were treated with 10-320(g/ml isorhamnetin, C57BL/6 mice were subcutaneously inoculated Lewis cells 0.2ml/each (1×107cells/ml) below the right forelimb armpit and were treated with 50 (g/ml isorhamnetin isorhamnetin.The results were observed and analyzed under light-microscope, electronic microscopy, growth inhibition was analyzed by MTT, clonogenic asssays and growth curve;the apoptosis and the expression-associated genes peaks were detected with flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay,
文摘The tendency of particles in a water-saturated granular mass to re-arrange into a denser state during cyclic shearing under pressure results in an increase in pore water pressure. The increase in the pore water pressure causes a reduction in the inner particle contact forces, and in turn easier re-arrangement of the particles. Eventually, the material loses its shear strength, partially or almost completely. In this paper, a general three-dimensional continuum mechanics model is presented for the deformation of granular materials. A physically based model is also presented for characterization of liquefaction of the water saturated granular material under undrained cyclic shearing. The model incorporates the fabric of the granular mass, which develops as the frictional granular mass is deformed in shear. It includes the coupling between shearing and excess pore water pressure. The model parameters are estimated, based on the results of cyclic shearing experiments on large hollow cylindrical samples of silica sand. Basically, the calculation results utilizing this model can embody liquefaction phenomena of the water saturated granular material under undrained cyclic shearing.
基金Supported by a grant from the Ph.D. Programs Foundation of Ministry of Education of China (No.20030003074) and the National Natural Science Foundation of China (No.10135040).
文摘In this paper we develop a general exact fan-beam reconstruction algorithm for free-form trajectories not only closed but also unclosed,based on the fan-beam reconstruction formula recently developed by Noo et al.. A mathematical proof is then provided with the geometrical explanation of equi-spatial detectors. With this algorithm we can obtain exact region of interest (ROI) reconstruction if and only if every projecting line passing through the ROI intersects the free-form source trajectory,when the projections are not truncated. Furthermore,under the condi- tion that the source-to-detectors distance changes slowly enough relative to the length itself,we obtain a very good approximate reconstruction algorithm,which is the same as the algorithm of the circular trajectory except that the source-to-detectors distance is a function of the rotation angle. Then the algorithms are tested using the Shepp-Logan phantom and the experiment shows that the algorithms can get perfect numerical results.
基金The images and the standard transformation were provided as part of the project,"Retrospective Im-age Registration Evaluation"(National Institutes of Health,1 R01 CA89323),the principal investigator,J.Michael Fitzpatrick,Vanderbilt Universi
文摘Mutual information has currently been one of the most intensivelyresearched measures. It has been proven to be accurate and effective registrationmeasure. Despite the general promising results, mutual information sometimes mightlead to misregistration because of neglecting spatial information and treating intensityvariations with undue sensitivity. In this paper, an extension of mutual informationframework was proposed in which higher-order spatial information regarding imagestructures was incorporated into the registration processing of PET and MR. Thesecond-order estimate of mutual information algorithm was applied to the registrationof seven patients. Evaluation from Vanderbilt University and our visual inspectionshowed that sub-voxel accuracy and robust results were achieved in all cases withsecond-order mutual information as the similarity measure and with Powell's multi-dimensional direction set method as optimization strategy.
文摘This paper presents a method for unsupervised segmentation of images consisting of multiple textures. The images under study are modeled by a proposed hierarchical random field model, which has two layers. The first layer is modeled as a Markov Random Field (MRF) representing an unobservable region image and the second layer uses 'Filters, Random and Maximum Entropy (Abb. FRAME)' model to represent multiple textures which cover each region. Compared with the traditional Hierarchical Markov Random Field (HMRF), the FRAME can use a bigger neighborhood system and model more complex patterns. The segmentation problem is formulated as Maximum a Posteriori (MAP) estimation according to the Bayesian rule. The iterated conditional modes (ICM) algorithm is carried out to find the solution of the MAP estimation. An algorithm based on the local entropy rate is proposed to simplify the estimation of the parameters of MRF. The parameters of FRAME are estimated by the ExpectationMaximum (EM) algorithm. Finally, an experiment with synthesized and real images is given, which shows that the method can segment images with complex textures efficiently and is robust to noise.
