Aim:To study the effect of prothymosin α(Pro Tα)as a fusion protein on the secetion of INF-γ,IFN-αand TNF-α in vitro.Methods:The in vitro study was carried out on the cultured of splenocytes,splenic and peritonea...Aim:To study the effect of prothymosin α(Pro Tα)as a fusion protein on the secetion of INF-γ,IFN-αand TNF-α in vitro.Methods:The in vitro study was carried out on the cultured of splenocytes,splenic and peritoneal macrophages isolated from Balb/c mice.Splenocytes were incubated with various concentrations of Pro Tα(1×10^-7-1×10^-10mol·L^-1) with or without Con A(5μg·mL^-1) for 72h.Splenic and peritoeal macrophages were respectively treated with Pro Tα(1×10^-7-1×10^-10mol.L^-1) in the presence of LPS(10μg· mL^-1) for 24h.Then INF-γ′s,INF-α′s and TNF-α′s levels in the supernatant were deteced by ELISA,Results:Pro Tα(1×10^-7)mol.L^-1) was found to obviously in crease INF-γ level(P<0.05) in the supernatant of splenocytes compared with the control group.Moreover,Pro Tα(1×10^-7mol.L^-1) significantly induced the secretion of INF-α(P<0.01) and TNF-α(P<0.01) in splenic and peritoneal macrophages.Conchusion:In vitro,Pro Tα could increase the secretion of IFN-γ,IFN-α and TNF-α.展开更多
AIM: To study the global gene expression of chemotactic genes in macrophage line U937 treated with human monocyte chemoattractant protein-1 (MCP-1) through the use of ExpreeChipTMO2 cDNA array. METHODS: Total RNA was ...AIM: To study the global gene expression of chemotactic genes in macrophage line U937 treated with human monocyte chemoattractant protein-1 (MCP-1) through the use of ExpreeChipTMO2 cDNA array. METHODS: Total RNA was extracted from MCP-1 treated macrophage line U937 and normal U937 cells, reversely transcribed to cDNA, and then screened in parallel with HO2 human cDNA array chip. The scanned result was additionally validated using RT-PCR. RESULTS: The result of cDNA array showed that one chemotactic-related gene was up-regulated more than two-fold (RANTES) and seven chemotactic-related genes were down-regulated more than two-fold (CCR1, CCR5, ccll6, GROβ, GROγ,IL-8 and granulocyte chemotactic protein 2) in MCP-1 treated U937 cells at mRNA level. RT-PCR analysis of four of these differentially expressed genes gave results consistent with cDNA array findings. CONCLUSION: MCP-1 could influence some chemokine and receptor expressions in macrophages in vitro. MCP-1 mainly down-regulates the expression of chemotactic genes influencing neutrophilic granulocyte expression (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2), and the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.展开更多
文摘Aim:To study the effect of prothymosin α(Pro Tα)as a fusion protein on the secetion of INF-γ,IFN-αand TNF-α in vitro.Methods:The in vitro study was carried out on the cultured of splenocytes,splenic and peritoneal macrophages isolated from Balb/c mice.Splenocytes were incubated with various concentrations of Pro Tα(1×10^-7-1×10^-10mol·L^-1) with or without Con A(5μg·mL^-1) for 72h.Splenic and peritoeal macrophages were respectively treated with Pro Tα(1×10^-7-1×10^-10mol.L^-1) in the presence of LPS(10μg· mL^-1) for 24h.Then INF-γ′s,INF-α′s and TNF-α′s levels in the supernatant were deteced by ELISA,Results:Pro Tα(1×10^-7)mol.L^-1) was found to obviously in crease INF-γ level(P<0.05) in the supernatant of splenocytes compared with the control group.Moreover,Pro Tα(1×10^-7mol.L^-1) significantly induced the secretion of INF-α(P<0.01) and TNF-α(P<0.01) in splenic and peritoneal macrophages.Conchusion:In vitro,Pro Tα could increase the secretion of IFN-γ,IFN-α and TNF-α.
文摘AIM: To study the global gene expression of chemotactic genes in macrophage line U937 treated with human monocyte chemoattractant protein-1 (MCP-1) through the use of ExpreeChipTMO2 cDNA array. METHODS: Total RNA was extracted from MCP-1 treated macrophage line U937 and normal U937 cells, reversely transcribed to cDNA, and then screened in parallel with HO2 human cDNA array chip. The scanned result was additionally validated using RT-PCR. RESULTS: The result of cDNA array showed that one chemotactic-related gene was up-regulated more than two-fold (RANTES) and seven chemotactic-related genes were down-regulated more than two-fold (CCR1, CCR5, ccll6, GROβ, GROγ,IL-8 and granulocyte chemotactic protein 2) in MCP-1 treated U937 cells at mRNA level. RT-PCR analysis of four of these differentially expressed genes gave results consistent with cDNA array findings. CONCLUSION: MCP-1 could influence some chemokine and receptor expressions in macrophages in vitro. MCP-1 mainly down-regulates the expression of chemotactic genes influencing neutrophilic granulocyte expression (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2), and the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.
