AIM:To investigate the antiangiogenic effects of endostatin on colonic carcinoma cell line implanted in nude mice and its mechanism. METHODS:Nude mice underwent subcutaneous injection with LS-174t colonic carcinoma ce...AIM:To investigate the antiangiogenic effects of endostatin on colonic carcinoma cell line implanted in nude mice and its mechanism. METHODS:Nude mice underwent subcutaneous injection with LS-174t colonic carcinoma cell line to generate carcinoma and were randomly separated into two groups.Mice received injection of vehicle or endostatin every day for two weeks. After the tumor was harvested,the tumor volumes were determined,and the expressions of CD34,VEGF and FIk-1 were examined by immunohistochemical method. RESULTS:Tumor volume was significantly inhibited in the endostatin group(84.17%)and tumor weight was significantly inhibited in the endostatin group(0.197±0.049) compared to the control group(1.198±0.105)(F=22.56, P=0.001),microvessel density(MVD)was significantly decreased in the treated group(31.857±3.515)compared to the control group(100.143±4.290)(F=151.62,P<0.001). Furthermore,the expression of FIk-1 was significantly inhibited in the treated group(34.29%) ompared to the control group(8.57%)(X^2=13.745,P=0.001).However no significant decrease was observed in the expression of vascular endothelial growth factor(VEGF)between these two groups(X^2=0.119,P=0.730). CONCLUSION:Endostatin can inhibit tumor growth and angiogenesis by blocking Vegf/FIk-1 pathway.This experiment provides the theory basis for developing a new anti-carcinoma drug through studying the properties of anti-angiogenesis inhibitors.展开更多
AIM: To investigate the expression of annexin I in pancreatic cancer and its relationship with the clinicopathologic factors,and to evaluate its potential clinical significance.METHODS: Annexin I expression was analyz...AIM: To investigate the expression of annexin I in pancreatic cancer and its relationship with the clinicopathologic factors,and to evaluate its potential clinical significance.METHODS: Annexin I expression was analyzed by Western blot and immunohistochemical staining in pancreatic adenocarcinoma and multi-tissue microarrays (MTAs).RESULTS: Western blot analysis showed that annexin I was overexpressed in 84.6% (11/13) pancreatic ductal adenocarcinomas. Immunohistochemistry analysis of pancreatic cancer in MTAs showed that annexin I protein was 71.4%(30/42) positive which was markedly increased compared with that in the tumor matched normal pancreas tissues 18.4%(7/38) (P<0.01). In the meantime, the high expression of annexin 1 was correlated with the poor differentiation of pancreatic adenocarcinoma.CONCLUSION: Annexin 1 overexpression is a frequent biological marker and correlates with the differentiation of pancreatic cancer during tumorigenesis.展开更多
AIM: To investigate the inhibitory effect of phosphorothioate anti-sense oligodeoxynucleotides (PASODN) on colorectal cancer LS-174T cells in vitro and the mechanism of inhibition of telomerase activity in these cells...AIM: To investigate the inhibitory effect of phosphorothioate anti-sense oligodeoxynucleotides (PASODN) on colorectal cancer LS-174T cells in vitro and the mechanism of inhibition of telomerase activity in these cells.METHODS: PASODN were used to infect LS-174T cells and block human telomerase RNA (hTR) through anti-sense technology. The inhibitory effect of PASODN was evaluated by colony-forming inhibition assay and growth curve. Changes of telomerase activity in LS-174T cells were detected by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA), and the level of apoptosis was analyzed by flow cytometry (FCM) assay.RESULTS: PASODN showed a dose and time-dependent inhibition of cell proliferation. The optimal dosage of PASODN was 10 limol/L The colony-forming efficiency was 10.3% in PASODN group after 10 d, whereas that in phosphorothioate mis-sense oligodeoxynucleotides (PMSODN) group with the same concentration and in PBS group (blank control) was 49.1% and 50.7%, respectively. PCR-ELISA results indicated that telomerase activity in the PASODN group was obviously inhibited in comparison with in the control groups (P<0.01,t= 3.317 and 3.241, to01(2o) = 2.845). Meanwhile, before the number of cells was decreased, the morphological changes were observed in the cells of PASODN group. The cells in PASODN group showed the apoptotic peak at 72 h after infection, whereas the control group did not show.CONCLUSION: Specific sequence oligonucleotides can inhibit telomerase activity and lead to cell apoptosis,suggesting a novel treatment strategy for malignant tumors induced by telomerase.展开更多
AIM: To assess the efficacy and safety of intraperitoneal chemotherapy in patients undergoing curative resection for gastric cancer through literature review.METHODS: Medline (PubMed) (1980-2003/i), Embase(1980-2003/1...AIM: To assess the efficacy and safety of intraperitoneal chemotherapy in patients undergoing curative resection for gastric cancer through literature review.METHODS: Medline (PubMed) (1980-2003/i), Embase(1980-2003/1), Cancerlit Database (1983-2003/1) and Chinese Biomedicine Database (1990-2003/1) were searched. Language was restricted to Chinese and English.The statistical analysis was performed by RevMan4.2 software provided by the Cochrane Collaboration. The results were expressed with odds ratio for the categorical variables.RESULTS: Eleven trials involving 1 161 cases were included.The pooled odds ratio was 0.51, with a 95% confidence interval (0.40-0.65). Intraperitoneal chemotherapy may benefit the patients after curative resection for locally advanced gastric cancer, and the combination of intraperitoneal chemotherapy with hyperthermia or activated carbon particles may provide more benefits to patients due to the enhanced antitumor activity of drugs. Sensitivity analysis and fail-safe number suggested that the result was comparatively reliable.However, of 11 trials, only 3 studies were of high quality.CONCLUSION: Intraperitoneal chemotherapy after curative resection for locally advanced gastric cancer may be beneficial to patients. Continuous multicenter, randomized,double blind, rigorously designed trials should be conducted to draw definitive conclusions.展开更多
AIM: To determine bhe accuracy of p53 gene mutations predicted by overexpression of p53 protein immunohistochemically,and to investigate the co-mutation of p53 and K-ras genes in rectal cancer and its effect on promot...AIM: To determine bhe accuracy of p53 gene mutations predicted by overexpression of p53 protein immunohistochemically,and to investigate the co-mutation of p53 and K-ras genes in rectal cancer and its effect on promoting malignant biologic behaviors of tumors.METHODS: Ninety-seven specimens of rectal cancer were surgically resected in our hospital from August 1996 to October 1997. The hot mutation areas of p53 gene (in exons 5-8) and K-rasgene (in codon 5/12 and 13) were detected with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and overexpression of p53 protein was detected with immunohistochemistry (IHC) in the 97 specimens of rectal cancer. Correlation between gene mutations and tumor clinicopathologic factors was studied, and survival analysis was penfomed as well.RESULTS: There were 36 cases of p53 gene mutations in 61 p53 protein positive cases, and 21 cases of p53 gene non-mutation in 36 p53 protein negative cases respectively.The coincidence rate of p53 gene mutation by IHC method with PCR-SSCP method was 58.8% (57/97). The mutation rate of p53 gene was 52.6% (51/97), while K-ras gene mutation was observed in codons 12 and 13 in 61 cases with a mutation rate of 62.9% (61/97). Single gene mutation of p53 or K-raswas found in 32 cases. Both p53 and K-ras gene mutation were found in 48 cases. Statistical analysis showed that p53 and K-ras gene mutations were not related to the dinicopathologic factors, including tumor size, gross tumor type, histological dassification, differentiation, invasion to intestinal veins, lymphatics and nerves, invasive depth to.wall, lymph node metastasis, and Dukes' stages (P>0.05).The survival in patients with no gene mutation, single gene mutation and both gene mutations were similar (P>0.05).CONCLUSION: IHC has a certain false positive and false negative rate in detecting p53 gene mutations. Malignant biological behaviours of rectal cancer are not enhanced by p53 and K-rasgene mutations. Co-mutation of p53 and K-ras gene has neither synergic carcinogenesis-promoting effect,nor prognostic effect on rectal cancer.展开更多
AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay....AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcriptionpolymerase chain reaction and the expression of p53 protein was examined by Western blotting.RESULTS: The reversal activity after treatment with wide type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.展开更多
OBJECTIVE To discuss the clinical efects of concomitant splenectomy in hepatocellular carcinoma patients accompanied with cirrhosis and hypersplenismMETHODS Sixty-seven patients who had hepatocellular carcinoma (HCC) ...OBJECTIVE To discuss the clinical efects of concomitant splenectomy in hepatocellular carcinoma patients accompanied with cirrhosis and hypersplenismMETHODS Sixty-seven patients who had hepatocellular carcinoma (HCC) accompanied with hypersplenism from December 1999 to March 2002 were reviewed retrospectively. Thirty-eight patients underwent liver and spleen united resection (splenectomy group) and 29 patients received a hepatectomy (non-splenectomy group).One day before operation and 7 days after operation, the concentration of vascular endothelium growth factor (VEGF) in peripheral blood and splenic venous blood were compared between the two groups.RESULTS The increase of PLT and WBC was significantly higher in patients who underwent concomitant splenectomy compared to patients who did not receive a splenectomy (P<0.05). The occurrence of complications was 28.9% (11/38) in the splenectomy group and 20.6% (6/29) in the nonsplenectomy group, and the recurrence rate one year later was 21.1 %(8/38) in the splenectomy group and 20.6%(6/29) in the non-splenectomy group. There was no significant difference in occurrence of complications and recurrence rates between the two groups. The concentration of VEGF was not significantly different between peripheral blood versus splenic venous blood. Twenty-nine patients in the splenectomy group received hepatic arterial chemoembolization 1-3 times successfully after operation, but in the non-splenectomy group there were 7 patients who had to stop receiving the successive treatment because the PLT and WBC were too low.CONCLUSION Combined splenectomy is helpful to raise the PLT and WBC count and enable patients to receive subsequent chemoembolization. Early recurrence and metastases are not significantly different between patients with and without splenectomy.展开更多
AIM: To investigate the potential role of cytokines in promoting Fas ligand (FasL)-expressing colon cancer cells. METHODS: Immunohistochemical SABC method was used to observe the expression of Fas receptor and ligand ...AIM: To investigate the potential role of cytokines in promoting Fas ligand (FasL)-expressing colon cancer cells. METHODS: Immunohistochemical SABC method was used to observe the expression of Fas receptor and ligand in SW620 colon cancer cell line and Jurkat T cells in order to provide the morphological evidence for the functions of Fas receptor and ligand. To examine the cytotoxicity of effector cells, CytoTox96 non-radioactive cytotoxicity assay was adopted to measure the lactate dehydrogenase-releasing value after SW620 cells were co-cultured with Jurkat T lymphocytes. RESULTS: The FasL of colon cancer SW620 cells was positive. The positive substances were distributed in the cell membrane and cytoplasm. The Fas receptor of colon cancer SW620 cells was negative. The Fas receptor and ligand of Jurkat T lymphocytes turned out to be positive. The positive substances were distributed in the cell membrane. After phytohemagglutinin (PHA)-stimulated Jurkat T lymphocytes were co-cultured with phorbol 12-myristate 13-acetate (PMA)-plus-ionomycin-stimulated (for 48 h) SW620 cells or tumor necrosis factor-alpha (TNF-α)-stimulated (for 48 h) SW620 cells or unstimulated SW620 cells for 4 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 10:1, 5:1, 2.5:1, and 1.25:1 was 74.6%, 40.8%, 32.4%, and 10.9% (F= 8.19, P<0.05); or 54.9%, 35.3%, 22.0%, and 10.3% (F= 11.12, P<0.05); or 14.9%, 10.5%, 6.9%, and 5.8% (F = 3.45, P<0.05). After PHA-stimulated Jurkat T lymphocytes were co-cultured with unstimulated SW620 cells for 8 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 5:1, 2.5:1, and 1.25:1 from the experiment was 83.9%, 74.1%, and 28.5% (F=137.04, P<0.05) respectively. Non-radioactive cytotoxicity assay showed that the apoptotic rate of Jurkat cells remarkably increased with the increase of planting concentration of SW620 cells and co-culture time after the SW620 cells were co-cultured with the Jurkat T lymphocytes. The cytotoxicity was significantly enhanced by PMA+ionomycin or TNF-α. CONCLUSION: The FasL expressed in human colon cancer cells may be regulated by endogenous factors in the microenvironment of the host and facilitate the escape of tumor cells from the host immune system.展开更多
AIM: To detect lymph nodes micrometastases and analyze its correlation with clinicopathological parameters in Dukes' A and B colorectal cancer patients.METHODS: One hundred and fourteen patients with colorectal ca...AIM: To detect lymph nodes micrometastases and analyze its correlation with clinicopathological parameters in Dukes' A and B colorectal cancer patients.METHODS: One hundred and fourteen patients with colorectal cancer (Dukes' A 16; Dukes' B 98) undergoing curative operation without histological lymph nodes metastases were studied between 2001 and 2003. A total of 2 481 lymph nodes were analyzed using monoclonal cytokeratin antibody AE1/AE3 (DAKO, Carpinteria, CA) for immunohistochemistry.RESULTS: In total, 33 (29%) patients were positive forcancer cell by immunohistochemistry. In 31 (94%) patientsof them positive nodes showed single tumor cell or small groups of tumor cells; and tumor deposits measuring 0.2and 0.37 mm in diameter in another 2 (6%) patients.Micrometastases were mainly located in the subcapsular sinus or paracortical sinus. There was no correlation between the positive lymph nodes and gender, age, tumor site, tumor size, histological type, histological grade, invasion depth, Dukes' staging and microsatellite instability (P＞0.05).CONCLUSION: Our findings suggest that immunohistochemical technique using monoclonal cytokeratin antibody AE1/AE3 may be a sensitive and reliable method for detecting lymph nodes micrometastases in Dukes' A and B colorectal cancer. The clinical significance of lymph nodes micrometastases is still not confirmed.展开更多
OBJECTIVE To study the relationship between the expression of VEGF, FIk-1 and Fit-1 proteins and clinical pathology in hepatocellular carcinoma.METHODS The expression of VEGF, FIk-1 and Fit-1 proteins in hepatocellula...OBJECTIVE To study the relationship between the expression of VEGF, FIk-1 and Fit-1 proteins and clinical pathology in hepatocellular carcinoma.METHODS The expression of VEGF, FIk-1 and Fit-1 proteins in hepatocellular carcinomas from 60 patients was determined by immunohistochemistry (ABC method) and VEGF expression in relation to the clinicopathology evaluated.RESULTS The positive rates of VEGF, FIk-1 and Fit-1 protein expression were 81.3%, 88.3%, 80.0% in tumor tissues, respectively, rates which were significantly higher than those in normal liver tissue (P<0.05). The expression of VEGF protein was correlated with the histologic grade and metastases of the tumors.CONCLUSION The results showed that, in hepatocellular carcinoma, a higher expression of VEGF protein was associated with a higher degree of malignancy and a greater tendency for metastases. VEGF, FIk-1 and Fit-1 play an important role in tumourgenesis.展开更多
OBJECTIVE To explore the clinico-pathological characteristics, diagnosis, treatment, and prognosis factors for primary breast malignant lymphoma (PBL). METHODS The clinical data from 21 cases of PBL were retrospective...OBJECTIVE To explore the clinico-pathological characteristics, diagnosis, treatment, and prognosis factors for primary breast malignant lymphoma (PBL). METHODS The clinical data from 21 cases of PBL were retrospectively analysed. RESULTS There were 19 females and 2 males with a median age of 37 years. All cases had diffuse non-Hodgkin lymphoma from B-cell lineage. The overall 5-year survival rate was 62.50% for the whole group and 81.82% for stage Ⅰ and Ⅱ diseases. CONCLUSION The prognosis of PBL is related to the stage and treatment modality. Operation combined with chemoradiotherapy is the best treatment method. Local resection should be the first surgical treatment.展开更多
Aim To study the method and clinical significancehepatic arterial and portal venous angiographyimplantable drug delivery system (IDDS).Methods Intraoperative hepatic artery and poaalcatheterization were performed on 5...Aim To study the method and clinical significancehepatic arterial and portal venous angiographyimplantable drug delivery system (IDDS).Methods Intraoperative hepatic artery and poaalcatheterization were performed on 56 patients withneoplasm.viaveinliverCatheters were connected with IDDS implanted inthe skin of abdominal wall, 76% meglueaminediatrzoatewas regularly injected via the IDDS to observe both vesselsand take desired spot films.Resulte Satisfactory mdiologieal manifestitions ofvessels and their branches were obtained, 5 casesintrahepatic cancer ous recurrence that were not discoveredby CT scan were detected, 4 by arteriography,pertovenography.with IDDS, andangiography.Conclusionthe other byNo obvious adverse effect was connectedno obvious complication occurredduringHepatic angiography via IDDS is a useful wayto monitor postoperative cancerous receurrenee in liver,also a new way to study the blood supply of hepatic neoplasmand blood hydrodynamics of peaal vein.展开更多
Aim To investigate the characteristics of portovenousradiology of primary hepatic carcinoma (PHC).Methods Intraoperative portovenous catheterization wereperformed on 51 patients with PHC. The catheters wereconnected w...Aim To investigate the characteristics of portovenousradiology of primary hepatic carcinoma (PHC).Methods Intraoperative portovenous catheterization wereperformed on 51 patients with PHC. The catheters wereconnected with implantible drug delivery system (IDDS)implanted in the skin of abdominal wall (adjacent to theincision cut). After operation, portovenography was doneand desired film were taken, then the radiologicalcharacteristics of portovenous system were analyzed.Resulte Satisfactory radiological manifestation ofpertovenous system was obtained. The main characteristicsof pertovenous system were: leaf-like sign caused byproliferation of small vessels near the major intrahepaticportal branches, portovenous branches stretched into the PHC lesion to participate its blood supply, thread-like sign,crab-like sign and goblet-rim sign or serpentine head signbecause of partial or total cancerous obstruction of portalveins, and manifestation of portal venous hypertensionconcomitant with PHC.Conclusion Radiological study of portal system of PHC isnot only useful to eonsummate the diagnosis of PHC, butalso helpful to direct the intervening treatment of it.展开更多
基金Supported by the Key Technologies Research and Development Program of Heilongjiang Province During the 9th Five-Year Plan Period,No.G99C 19-5
文摘AIM:To investigate the antiangiogenic effects of endostatin on colonic carcinoma cell line implanted in nude mice and its mechanism. METHODS:Nude mice underwent subcutaneous injection with LS-174t colonic carcinoma cell line to generate carcinoma and were randomly separated into two groups.Mice received injection of vehicle or endostatin every day for two weeks. After the tumor was harvested,the tumor volumes were determined,and the expressions of CD34,VEGF and FIk-1 were examined by immunohistochemical method. RESULTS:Tumor volume was significantly inhibited in the endostatin group(84.17%)and tumor weight was significantly inhibited in the endostatin group(0.197±0.049) compared to the control group(1.198±0.105)(F=22.56, P=0.001),microvessel density(MVD)was significantly decreased in the treated group(31.857±3.515)compared to the control group(100.143±4.290)(F=151.62,P<0.001). Furthermore,the expression of FIk-1 was significantly inhibited in the treated group(34.29%) ompared to the control group(8.57%)(X^2=13.745,P=0.001).However no significant decrease was observed in the expression of vascular endothelial growth factor(VEGF)between these two groups(X^2=0.119,P=0.730). CONCLUSION:Endostatin can inhibit tumor growth and angiogenesis by blocking Vegf/FIk-1 pathway.This experiment provides the theory basis for developing a new anti-carcinoma drug through studying the properties of anti-angiogenesis inhibitors.
基金Supported by National Natural Science Foundation of China NO.30240050,30225045,39990570,30171049,and 30370713 and National High Tech R & D Program of China,2001AA227091
文摘AIM: To investigate the expression of annexin I in pancreatic cancer and its relationship with the clinicopathologic factors,and to evaluate its potential clinical significance.METHODS: Annexin I expression was analyzed by Western blot and immunohistochemical staining in pancreatic adenocarcinoma and multi-tissue microarrays (MTAs).RESULTS: Western blot analysis showed that annexin I was overexpressed in 84.6% (11/13) pancreatic ductal adenocarcinomas. Immunohistochemistry analysis of pancreatic cancer in MTAs showed that annexin I protein was 71.4%(30/42) positive which was markedly increased compared with that in the tumor matched normal pancreas tissues 18.4%(7/38) (P<0.01). In the meantime, the high expression of annexin 1 was correlated with the poor differentiation of pancreatic adenocarcinoma.CONCLUSION: Annexin 1 overexpression is a frequent biological marker and correlates with the differentiation of pancreatic cancer during tumorigenesis.
基金Supported by the Youth Science Foundation of Heilongjiang Province,China,No.QC01C08,and Heilongjiang Provincial Technology Council Foundations,China,No.GC03C605-6
文摘AIM: To investigate the inhibitory effect of phosphorothioate anti-sense oligodeoxynucleotides (PASODN) on colorectal cancer LS-174T cells in vitro and the mechanism of inhibition of telomerase activity in these cells.METHODS: PASODN were used to infect LS-174T cells and block human telomerase RNA (hTR) through anti-sense technology. The inhibitory effect of PASODN was evaluated by colony-forming inhibition assay and growth curve. Changes of telomerase activity in LS-174T cells were detected by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA), and the level of apoptosis was analyzed by flow cytometry (FCM) assay.RESULTS: PASODN showed a dose and time-dependent inhibition of cell proliferation. The optimal dosage of PASODN was 10 limol/L The colony-forming efficiency was 10.3% in PASODN group after 10 d, whereas that in phosphorothioate mis-sense oligodeoxynucleotides (PMSODN) group with the same concentration and in PBS group (blank control) was 49.1% and 50.7%, respectively. PCR-ELISA results indicated that telomerase activity in the PASODN group was obviously inhibited in comparison with in the control groups (P<0.01,t= 3.317 and 3.241, to01(2o) = 2.845). Meanwhile, before the number of cells was decreased, the morphological changes were observed in the cells of PASODN group. The cells in PASODN group showed the apoptotic peak at 72 h after infection, whereas the control group did not show.CONCLUSION: Specific sequence oligonucleotides can inhibit telomerase activity and lead to cell apoptosis,suggesting a novel treatment strategy for malignant tumors induced by telomerase.
基金Supported by the Natural Science Foundationof Guangdong Province,No.20030245
文摘AIM: To assess the efficacy and safety of intraperitoneal chemotherapy in patients undergoing curative resection for gastric cancer through literature review.METHODS: Medline (PubMed) (1980-2003/i), Embase(1980-2003/1), Cancerlit Database (1983-2003/1) and Chinese Biomedicine Database (1990-2003/1) were searched. Language was restricted to Chinese and English.The statistical analysis was performed by RevMan4.2 software provided by the Cochrane Collaboration. The results were expressed with odds ratio for the categorical variables.RESULTS: Eleven trials involving 1 161 cases were included.The pooled odds ratio was 0.51, with a 95% confidence interval (0.40-0.65). Intraperitoneal chemotherapy may benefit the patients after curative resection for locally advanced gastric cancer, and the combination of intraperitoneal chemotherapy with hyperthermia or activated carbon particles may provide more benefits to patients due to the enhanced antitumor activity of drugs. Sensitivity analysis and fail-safe number suggested that the result was comparatively reliable.However, of 11 trials, only 3 studies were of high quality.CONCLUSION: Intraperitoneal chemotherapy after curative resection for locally advanced gastric cancer may be beneficial to patients. Continuous multicenter, randomized,double blind, rigorously designed trials should be conducted to draw definitive conclusions.
文摘AIM: To determine bhe accuracy of p53 gene mutations predicted by overexpression of p53 protein immunohistochemically,and to investigate the co-mutation of p53 and K-ras genes in rectal cancer and its effect on promoting malignant biologic behaviors of tumors.METHODS: Ninety-seven specimens of rectal cancer were surgically resected in our hospital from August 1996 to October 1997. The hot mutation areas of p53 gene (in exons 5-8) and K-rasgene (in codon 5/12 and 13) were detected with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and overexpression of p53 protein was detected with immunohistochemistry (IHC) in the 97 specimens of rectal cancer. Correlation between gene mutations and tumor clinicopathologic factors was studied, and survival analysis was penfomed as well.RESULTS: There were 36 cases of p53 gene mutations in 61 p53 protein positive cases, and 21 cases of p53 gene non-mutation in 36 p53 protein negative cases respectively.The coincidence rate of p53 gene mutation by IHC method with PCR-SSCP method was 58.8% (57/97). The mutation rate of p53 gene was 52.6% (51/97), while K-ras gene mutation was observed in codons 12 and 13 in 61 cases with a mutation rate of 62.9% (61/97). Single gene mutation of p53 or K-raswas found in 32 cases. Both p53 and K-ras gene mutation were found in 48 cases. Statistical analysis showed that p53 and K-ras gene mutations were not related to the dinicopathologic factors, including tumor size, gross tumor type, histological dassification, differentiation, invasion to intestinal veins, lymphatics and nerves, invasive depth to.wall, lymph node metastasis, and Dukes' stages (P>0.05).The survival in patients with no gene mutation, single gene mutation and both gene mutations were similar (P>0.05).CONCLUSION: IHC has a certain false positive and false negative rate in detecting p53 gene mutations. Malignant biological behaviours of rectal cancer are not enhanced by p53 and K-rasgene mutations. Co-mutation of p53 and K-ras gene has neither synergic carcinogenesis-promoting effect,nor prognostic effect on rectal cancer.
文摘AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcriptionpolymerase chain reaction and the expression of p53 protein was examined by Western blotting.RESULTS: The reversal activity after treatment with wide type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.
文摘OBJECTIVE To discuss the clinical efects of concomitant splenectomy in hepatocellular carcinoma patients accompanied with cirrhosis and hypersplenismMETHODS Sixty-seven patients who had hepatocellular carcinoma (HCC) accompanied with hypersplenism from December 1999 to March 2002 were reviewed retrospectively. Thirty-eight patients underwent liver and spleen united resection (splenectomy group) and 29 patients received a hepatectomy (non-splenectomy group).One day before operation and 7 days after operation, the concentration of vascular endothelium growth factor (VEGF) in peripheral blood and splenic venous blood were compared between the two groups.RESULTS The increase of PLT and WBC was significantly higher in patients who underwent concomitant splenectomy compared to patients who did not receive a splenectomy (P<0.05). The occurrence of complications was 28.9% (11/38) in the splenectomy group and 20.6% (6/29) in the nonsplenectomy group, and the recurrence rate one year later was 21.1 %(8/38) in the splenectomy group and 20.6%(6/29) in the non-splenectomy group. There was no significant difference in occurrence of complications and recurrence rates between the two groups. The concentration of VEGF was not significantly different between peripheral blood versus splenic venous blood. Twenty-nine patients in the splenectomy group received hepatic arterial chemoembolization 1-3 times successfully after operation, but in the non-splenectomy group there were 7 patients who had to stop receiving the successive treatment because the PLT and WBC were too low.CONCLUSION Combined splenectomy is helpful to raise the PLT and WBC count and enable patients to receive subsequent chemoembolization. Early recurrence and metastases are not significantly different between patients with and without splenectomy.
基金Supported by the Science and Technology Commission Foundation of Tianjin, No. 003119711
文摘AIM: To investigate the potential role of cytokines in promoting Fas ligand (FasL)-expressing colon cancer cells. METHODS: Immunohistochemical SABC method was used to observe the expression of Fas receptor and ligand in SW620 colon cancer cell line and Jurkat T cells in order to provide the morphological evidence for the functions of Fas receptor and ligand. To examine the cytotoxicity of effector cells, CytoTox96 non-radioactive cytotoxicity assay was adopted to measure the lactate dehydrogenase-releasing value after SW620 cells were co-cultured with Jurkat T lymphocytes. RESULTS: The FasL of colon cancer SW620 cells was positive. The positive substances were distributed in the cell membrane and cytoplasm. The Fas receptor of colon cancer SW620 cells was negative. The Fas receptor and ligand of Jurkat T lymphocytes turned out to be positive. The positive substances were distributed in the cell membrane. After phytohemagglutinin (PHA)-stimulated Jurkat T lymphocytes were co-cultured with phorbol 12-myristate 13-acetate (PMA)-plus-ionomycin-stimulated (for 48 h) SW620 cells or tumor necrosis factor-alpha (TNF-α)-stimulated (for 48 h) SW620 cells or unstimulated SW620 cells for 4 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 10:1, 5:1, 2.5:1, and 1.25:1 was 74.6%, 40.8%, 32.4%, and 10.9% (F= 8.19, P<0.05); or 54.9%, 35.3%, 22.0%, and 10.3% (F= 11.12, P<0.05); or 14.9%, 10.5%, 6.9%, and 5.8% (F = 3.45, P<0.05). After PHA-stimulated Jurkat T lymphocytes were co-cultured with unstimulated SW620 cells for 8 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 5:1, 2.5:1, and 1.25:1 from the experiment was 83.9%, 74.1%, and 28.5% (F=137.04, P<0.05) respectively. Non-radioactive cytotoxicity assay showed that the apoptotic rate of Jurkat cells remarkably increased with the increase of planting concentration of SW620 cells and co-culture time after the SW620 cells were co-cultured with the Jurkat T lymphocytes. The cytotoxicity was significantly enhanced by PMA+ionomycin or TNF-α. CONCLUSION: The FasL expressed in human colon cancer cells may be regulated by endogenous factors in the microenvironment of the host and facilitate the escape of tumor cells from the host immune system.
文摘AIM: To detect lymph nodes micrometastases and analyze its correlation with clinicopathological parameters in Dukes' A and B colorectal cancer patients.METHODS: One hundred and fourteen patients with colorectal cancer (Dukes' A 16; Dukes' B 98) undergoing curative operation without histological lymph nodes metastases were studied between 2001 and 2003. A total of 2 481 lymph nodes were analyzed using monoclonal cytokeratin antibody AE1/AE3 (DAKO, Carpinteria, CA) for immunohistochemistry.RESULTS: In total, 33 (29%) patients were positive forcancer cell by immunohistochemistry. In 31 (94%) patientsof them positive nodes showed single tumor cell or small groups of tumor cells; and tumor deposits measuring 0.2and 0.37 mm in diameter in another 2 (6%) patients.Micrometastases were mainly located in the subcapsular sinus or paracortical sinus. There was no correlation between the positive lymph nodes and gender, age, tumor site, tumor size, histological type, histological grade, invasion depth, Dukes' staging and microsatellite instability (P＞0.05).CONCLUSION: Our findings suggest that immunohistochemical technique using monoclonal cytokeratin antibody AE1/AE3 may be a sensitive and reliable method for detecting lymph nodes micrometastases in Dukes' A and B colorectal cancer. The clinical significance of lymph nodes micrometastases is still not confirmed.
文摘OBJECTIVE To study the relationship between the expression of VEGF, FIk-1 and Fit-1 proteins and clinical pathology in hepatocellular carcinoma.METHODS The expression of VEGF, FIk-1 and Fit-1 proteins in hepatocellular carcinomas from 60 patients was determined by immunohistochemistry (ABC method) and VEGF expression in relation to the clinicopathology evaluated.RESULTS The positive rates of VEGF, FIk-1 and Fit-1 protein expression were 81.3%, 88.3%, 80.0% in tumor tissues, respectively, rates which were significantly higher than those in normal liver tissue (P<0.05). The expression of VEGF protein was correlated with the histologic grade and metastases of the tumors.CONCLUSION The results showed that, in hepatocellular carcinoma, a higher expression of VEGF protein was associated with a higher degree of malignancy and a greater tendency for metastases. VEGF, FIk-1 and Fit-1 play an important role in tumourgenesis.
文摘OBJECTIVE To explore the clinico-pathological characteristics, diagnosis, treatment, and prognosis factors for primary breast malignant lymphoma (PBL). METHODS The clinical data from 21 cases of PBL were retrospectively analysed. RESULTS There were 19 females and 2 males with a median age of 37 years. All cases had diffuse non-Hodgkin lymphoma from B-cell lineage. The overall 5-year survival rate was 62.50% for the whole group and 81.82% for stage Ⅰ and Ⅱ diseases. CONCLUSION The prognosis of PBL is related to the stage and treatment modality. Operation combined with chemoradiotherapy is the best treatment method. Local resection should be the first surgical treatment.
文摘Aim To study the method and clinical significancehepatic arterial and portal venous angiographyimplantable drug delivery system (IDDS).Methods Intraoperative hepatic artery and poaalcatheterization were performed on 56 patients withneoplasm.viaveinliverCatheters were connected with IDDS implanted inthe skin of abdominal wall, 76% meglueaminediatrzoatewas regularly injected via the IDDS to observe both vesselsand take desired spot films.Resulte Satisfactory mdiologieal manifestitions ofvessels and their branches were obtained, 5 casesintrahepatic cancer ous recurrence that were not discoveredby CT scan were detected, 4 by arteriography,pertovenography.with IDDS, andangiography.Conclusionthe other byNo obvious adverse effect was connectedno obvious complication occurredduringHepatic angiography via IDDS is a useful wayto monitor postoperative cancerous receurrenee in liver,also a new way to study the blood supply of hepatic neoplasmand blood hydrodynamics of peaal vein.
文摘Aim To investigate the characteristics of portovenousradiology of primary hepatic carcinoma (PHC).Methods Intraoperative portovenous catheterization wereperformed on 51 patients with PHC. The catheters wereconnected with implantible drug delivery system (IDDS)implanted in the skin of abdominal wall (adjacent to theincision cut). After operation, portovenography was doneand desired film were taken, then the radiologicalcharacteristics of portovenous system were analyzed.Resulte Satisfactory radiological manifestation ofpertovenous system was obtained. The main characteristicsof pertovenous system were: leaf-like sign caused byproliferation of small vessels near the major intrahepaticportal branches, portovenous branches stretched into the PHC lesion to participate its blood supply, thread-like sign,crab-like sign and goblet-rim sign or serpentine head signbecause of partial or total cancerous obstruction of portalveins, and manifestation of portal venous hypertensionconcomitant with PHC.Conclusion Radiological study of portal system of PHC isnot only useful to eonsummate the diagnosis of PHC, butalso helpful to direct the intervening treatment of it.
