Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitorin...Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitoring.Impact Statement:The integration of Ti_(3)C_(2)T_(x)-MXene into BC matrices represents a novel approach to creating multifunctional hydrogels that combine biocompatibility,electrical conductivity,and mechanical durability.These properties make the hydrogels promising candidates for advanced wound care and real-time monitoring applications.Introduction:Wound healing requires materials that support cell growth,promote tissue regeneration,and enable real-time monitoring.MXenes,a class of 2-dimensional materials,offer unique electrical and mechanical properties,making them suitable for biomedical applications.This study explores the integration of Ti_(3)C_(2)T_(x)-MXene with BC,a biopolymer known for its excellent biocompatibility and mechanical strength,to create electroactive composite hydrogel films for advanced wound care.Methods:Ti_(3)C_(2)T_(x)-MXene was synthesized by etching Ti3AlC2 with hydrofluoric acid and integrated into BC pellicles produced by Gluconacetobacter xylinum.The composite hydrogel films underwent characterization through x-ray diffraction(XRD),x-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and thermogravimetric analysis(TGA)to determine structural,chemical,and thermal properties.Mechanical testing assessed tensile and compressive strengths.Biological assessments,including cell viability,hemolysis rate,and protein expression,evaluated biocompatibility and regenerative potential.Results:XRD confirmed the crystallographic structure of MXene and BC composite film.XPS and FTIR validated the successful incorporation of MXene into the film matrix.Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive strength of 4.2 MPa.TGA showed stability up to 350℃,and the electrical conductivity reached 9.14×10^(−4)S/m,enabling real-time monitoring capabilities.Cell viability exceeded 95%,with a hemolysis rate below 2%.Protein expression studies revealed the ability to promote skin regeneration through collagen I,K10,K5,and filaggrin expression.Conclusion:The BC/MXene composite hydrogel films exhibit important potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring.Their unique combination of mechanical durability,electrical conductivity,and biocompatibility highlights their promise for advanced wound care applications.展开更多
文摘Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitoring.Impact Statement:The integration of Ti_(3)C_(2)T_(x)-MXene into BC matrices represents a novel approach to creating multifunctional hydrogels that combine biocompatibility,electrical conductivity,and mechanical durability.These properties make the hydrogels promising candidates for advanced wound care and real-time monitoring applications.Introduction:Wound healing requires materials that support cell growth,promote tissue regeneration,and enable real-time monitoring.MXenes,a class of 2-dimensional materials,offer unique electrical and mechanical properties,making them suitable for biomedical applications.This study explores the integration of Ti_(3)C_(2)T_(x)-MXene with BC,a biopolymer known for its excellent biocompatibility and mechanical strength,to create electroactive composite hydrogel films for advanced wound care.Methods:Ti_(3)C_(2)T_(x)-MXene was synthesized by etching Ti3AlC2 with hydrofluoric acid and integrated into BC pellicles produced by Gluconacetobacter xylinum.The composite hydrogel films underwent characterization through x-ray diffraction(XRD),x-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and thermogravimetric analysis(TGA)to determine structural,chemical,and thermal properties.Mechanical testing assessed tensile and compressive strengths.Biological assessments,including cell viability,hemolysis rate,and protein expression,evaluated biocompatibility and regenerative potential.Results:XRD confirmed the crystallographic structure of MXene and BC composite film.XPS and FTIR validated the successful incorporation of MXene into the film matrix.Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive strength of 4.2 MPa.TGA showed stability up to 350℃,and the electrical conductivity reached 9.14×10^(−4)S/m,enabling real-time monitoring capabilities.Cell viability exceeded 95%,with a hemolysis rate below 2%.Protein expression studies revealed the ability to promote skin regeneration through collagen I,K10,K5,and filaggrin expression.Conclusion:The BC/MXene composite hydrogel films exhibit important potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring.Their unique combination of mechanical durability,electrical conductivity,and biocompatibility highlights their promise for advanced wound care applications.
