Dear Editor,Type 2 diabetes mellitus(T2DM)has been identified as a risk factor for increased severity in acute pancreatitis(AP)(Mikóet al,2018).However,to date,no studies have simultaneously included healthy indi...Dear Editor,Type 2 diabetes mellitus(T2DM)has been identified as a risk factor for increased severity in acute pancreatitis(AP)(Mikóet al,2018).However,to date,no studies have simultaneously included healthy individuals,T2DM patients,and AP patients to investigate which diabetes-associated metabolites are linked to AP severity.展开更多
Beyond glycemic control,applications of glucagon-like peptide-1 receptor(GLP-1r)agonists(GLP-1 RAs)inhibit inflammationand plaque development in murine atherosclerotic models.However,whether they modulate hematopoieti...Beyond glycemic control,applications of glucagon-like peptide-1 receptor(GLP-1r)agonists(GLP-1 RAs)inhibit inflammationand plaque development in murine atherosclerotic models.However,whether they modulate hematopoietic stem/progenitor cells(HSPCs)to prohibit skewed myelopoiesis in hypercholesteremia remains unknown.In this study,GLP-1r expression in fluorescenceactivated cell sorting(FACS)-sorted wild-type HSPCs was determined by capillary western blotting.Bone marrow cells(BMCs)of wild-type or GLP-1r−/−mice were transplanted into lethally irradiated low-density lipoprotein receptor deficient(LDLr−/−)recipients followed by high-fat diet(HFD)for chimerism analysis by FACS.In parallel,LDLr−/−mice were placed on HFD for 6weeks and then treated with saline or Exendin-4(Ex-4)for another 6 weeks.HSPC frequency and cell cycle were analyzed byFACS,and intracellular metabolite levels were assessed by targeted metabolomics.The results demonstrated that HSPCs expressedGLP-1r and transplantation of GLP-1r−/−BMCs resulted in skewed myelopoiesis in hypercholesterolemic LDLr−/−recipients.In vitro,Ex-4 treatment of FACS-purified HSPCs suppressed cell expansion and granulocyte production induced by LDL.In vivo,Ex-4treatment inhibited plaque progression,suppressed HSPC proliferation,and modified glycolytic and lipid metabolism in HSPCs ofhypercholesteremic LDLr−/−mice.In conclusion,Ex-4 could directly inhibit HSPC proliferation induced by hypercholesteremia.展开更多
To the Editor:The emergence of Omicron has gained global attention since November 2021.Of the Omicron strains,Omicron XBB.1.5 is the most recently identified.It carries deletions and more than 30 mutations,which have ...To the Editor:The emergence of Omicron has gained global attention since November 2021.Of the Omicron strains,Omicron XBB.1.5 is the most recently identified.It carries deletions and more than 30 mutations,which have modified the biological features of severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)for enhanced transmissibility and immune escape capacity.展开更多
基金supported by the National Natural Science Foundation of China(82070841 to Yingmei Feng,82200715 to Yuan Dong,32321004 and 92357308 to Guanghou Shui).
文摘Dear Editor,Type 2 diabetes mellitus(T2DM)has been identified as a risk factor for increased severity in acute pancreatitis(AP)(Mikóet al,2018).However,to date,no studies have simultaneously included healthy individuals,T2DM patients,and AP patients to investigate which diabetes-associated metabolites are linked to AP severity.
基金supported by grants from the National Natural Science Foundation of China(81670765 and 82070841)to Y.F.
文摘Beyond glycemic control,applications of glucagon-like peptide-1 receptor(GLP-1r)agonists(GLP-1 RAs)inhibit inflammationand plaque development in murine atherosclerotic models.However,whether they modulate hematopoietic stem/progenitor cells(HSPCs)to prohibit skewed myelopoiesis in hypercholesteremia remains unknown.In this study,GLP-1r expression in fluorescenceactivated cell sorting(FACS)-sorted wild-type HSPCs was determined by capillary western blotting.Bone marrow cells(BMCs)of wild-type or GLP-1r−/−mice were transplanted into lethally irradiated low-density lipoprotein receptor deficient(LDLr−/−)recipients followed by high-fat diet(HFD)for chimerism analysis by FACS.In parallel,LDLr−/−mice were placed on HFD for 6weeks and then treated with saline or Exendin-4(Ex-4)for another 6 weeks.HSPC frequency and cell cycle were analyzed byFACS,and intracellular metabolite levels were assessed by targeted metabolomics.The results demonstrated that HSPCs expressedGLP-1r and transplantation of GLP-1r−/−BMCs resulted in skewed myelopoiesis in hypercholesterolemic LDLr−/−recipients.In vitro,Ex-4 treatment of FACS-purified HSPCs suppressed cell expansion and granulocyte production induced by LDL.In vivo,Ex-4treatment inhibited plaque progression,suppressed HSPC proliferation,and modified glycolytic and lipid metabolism in HSPCs ofhypercholesteremic LDLr−/−mice.In conclusion,Ex-4 could directly inhibit HSPC proliferation induced by hypercholesteremia.
基金supported by a grant from the National Key R&D Program of China(No.2023YFC0872400).
文摘To the Editor:The emergence of Omicron has gained global attention since November 2021.Of the Omicron strains,Omicron XBB.1.5 is the most recently identified.It carries deletions and more than 30 mutations,which have modified the biological features of severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)for enhanced transmissibility and immune escape capacity.
