Oral lichen planus (OLP) is a disease of unknown etiology affecting oral mucosa by mediated chronic inflammation and is classified as a potentially malignant oral disorder. SOCS1 and SOCS3 in the SOCS family have been...Oral lichen planus (OLP) is a disease of unknown etiology affecting oral mucosa by mediated chronic inflammation and is classified as a potentially malignant oral disorder. SOCS1 and SOCS3 in the SOCS family have been identified as negative regulators of the cytokine-activated JAK/STAT pathway responsible for inflammatory reaction. The DNA methylation in the promoter regions of SOCS1 and SOCS3 have been reported to correlate with carcinogenesis. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of the promoter regions in SOCS1 and SOCS3 genes using biopsy samples from OLP and oral squamous cell carcinoma (OSCC) patients. SOCS1 was methylated in 14/29 (48.3%) cases with OLP and 7/15 (46.7%) cases with OSCC. At the same time, SOCS3 was methylated in 25/29 (86.2%) cases with OLP and 11/15 (73.3%) cases with OSCC. We didn’t recognize any DNA methylation in SOCS1 or SOCS3 genes from the exfoliated cytological specimens of normal buccal mucosa. Furthermore, mRNA expression level was analyzed using real-time RT-PCR method to evaluate the correlation with DNA methylation status. DNA methylation status of SOCS1 seemed not to affect the expression level of SOCS1 mRNA. At the same time, DNA methylation status of SOCS3 was negatively correlated with the expression level of SOCS3 mRNA (p < 0.05). We posit frequent methylation of the SOCS3 gene promoter, theoretically resulting in the increase of cytokines expression, might be associated with the etiological mechanism of OLP.展开更多
We combine the de Broglie Matter Wave Equation with the Heisenberg Uncertainty Principle to derive an equation for time as a wave. This happens to be the first time that these two statements have been combined in this...We combine the de Broglie Matter Wave Equation with the Heisenberg Uncertainty Principle to derive an equation for time as a wave. This happens to be the first time that these two statements have been combined in this manner to derive an equation for time. The result is astounding. Time turns out to be a minuscule blob of quantum electromagnetic energy in perpetual angular momentum. From this time equation, we derive an equation for space which turns out to also predict a string (like the string of string theory). We then combine the time equation with the space equation to derive an equation for the inverse of quantum gravity which is also surprisingly electromagnetic in nature. This last statement implies that space is multidimensional and gravity in multidimensional space is not quantized, but its inverse (which is single-dimensional) is.展开更多
文摘Oral lichen planus (OLP) is a disease of unknown etiology affecting oral mucosa by mediated chronic inflammation and is classified as a potentially malignant oral disorder. SOCS1 and SOCS3 in the SOCS family have been identified as negative regulators of the cytokine-activated JAK/STAT pathway responsible for inflammatory reaction. The DNA methylation in the promoter regions of SOCS1 and SOCS3 have been reported to correlate with carcinogenesis. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of the promoter regions in SOCS1 and SOCS3 genes using biopsy samples from OLP and oral squamous cell carcinoma (OSCC) patients. SOCS1 was methylated in 14/29 (48.3%) cases with OLP and 7/15 (46.7%) cases with OSCC. At the same time, SOCS3 was methylated in 25/29 (86.2%) cases with OLP and 11/15 (73.3%) cases with OSCC. We didn’t recognize any DNA methylation in SOCS1 or SOCS3 genes from the exfoliated cytological specimens of normal buccal mucosa. Furthermore, mRNA expression level was analyzed using real-time RT-PCR method to evaluate the correlation with DNA methylation status. DNA methylation status of SOCS1 seemed not to affect the expression level of SOCS1 mRNA. At the same time, DNA methylation status of SOCS3 was negatively correlated with the expression level of SOCS3 mRNA (p < 0.05). We posit frequent methylation of the SOCS3 gene promoter, theoretically resulting in the increase of cytokines expression, might be associated with the etiological mechanism of OLP.
文摘We combine the de Broglie Matter Wave Equation with the Heisenberg Uncertainty Principle to derive an equation for time as a wave. This happens to be the first time that these two statements have been combined in this manner to derive an equation for time. The result is astounding. Time turns out to be a minuscule blob of quantum electromagnetic energy in perpetual angular momentum. From this time equation, we derive an equation for space which turns out to also predict a string (like the string of string theory). We then combine the time equation with the space equation to derive an equation for the inverse of quantum gravity which is also surprisingly electromagnetic in nature. This last statement implies that space is multidimensional and gravity in multidimensional space is not quantized, but its inverse (which is single-dimensional) is.
