To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal cancer.METHODSIn this study, we included forty-eight patients with primary colorectal...To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal cancer.METHODSIn this study, we included forty-eight patients with primary colorectal cancer and nine patients for control tissue from the excision of a colonic segment for benign conditions. We determined the clinicopathological features and evaluated the immunohistochemical expression pattern of B2A receptors as well as the morphological changes of the enteric nervous system (ENS). In order to assess statistical differences, we used the student t-test for comparing the means of two groups and one-way analysis of variance with Bonferroni’s post hoc analysis for comparing the means of more than two groups. Correlations were assessed using the Pearson’s correlation coefficient.RESULTSB2A receptors were significantly associated with tumor grading, tumor size, tumor invasion, lymph node metastasis (P < 0.05), while there were no statistically significant associations with gender, CRC location and gross appearance (P > 0.05). We observed, on one hand, a decrease of the relative area for both Auerbach and Meissner plexuses with the increase of the tumor grading, and on the other hand, an increase of the relative area of other nervous elements not in the Meissner plexus or in the Auerbach plexus with the tumor grading. For G1 tumors we found that epithelial B2A area showed an inverse correlation with the Auerbach plexus areas [r(14) = -0.531, P < 0.05], while for G2 tumors, epithelial B2A areas showed an indirect variation with both the Auerbach plexus areas [r(14) = -0.453, P < 0.05] and the Meissner areas [r(14) = -0.825, P < 0.01]. For G3 tumors, the inverse dependence increased for both Auerbach [r(14) = -0.587, P < 0.05] and Meissner [r(14) = -0.934, P < 0.05] plexuses.CONCLUSIONB2A receptors play an important role in colorectal carcinogenesis and can be utilized as prognostic factors. Furthermore, study of the ENS in colorectal cancer may lead to targeted molecular therapies.展开更多
Water quality and occurrence of water-borne diseases in the Gaza strip are vivid examples for most developing societies. In recent years, the quality and quantity of groundwater, the only source of waters in the Gaza ...Water quality and occurrence of water-borne diseases in the Gaza strip are vivid examples for most developing societies. In recent years, the quality and quantity of groundwater, the only source of waters in the Gaza strip, have deteriorated markedly. A general rundown of the infrastructure and water distribution networks, in particular, the spread of cesspools, excessive use of pesticides and fertilizers, and improper treatment and disposal of wastewater remain major contributing factors to the continued deterioration in the water status in the Gaza strip. Without a doubt, the (Israeli)-Palestinian conflict had a clear negative impact on the water sector in the Gaza strip. Apparently, there is a dire need to adopt the WHO’s water safety plan in the management of Gaza’s water supply systems from catchment to consumer’s tap in order to maintain the sustainability and quality of water resources and prevent outbreaks of waterborne diseases. Therefore, this review has been prepared to highlight the overall picture of the water dilemma in the Gaza strip in the last years and in addition, to identify the sources, sorts, levels, and health risks of consequence to microbial contamination of water. The impact of political conflicts on the water sector in the Gaza strip also was reviewed. Furthermore, recommendations were formulated in order to assist and guide future researchers, stakeholders, and policymakers to avoid the more exacerbation of water contamination as well as to protect public health.展开更多
This paper aimed to describe and explain the expectations concerning the use of music in NICU from the viewpoints of parents, nurses and physicians. The relevant questionnaires were distributed to 836 participants who...This paper aimed to describe and explain the expectations concerning the use of music in NICU from the viewpoints of parents, nurses and physicians. The relevant questionnaires were distributed to 836 participants who were recruited from the country’s five university hospitals (n = 508, response rate 61). Slightly over half of the participants preferred recorded music versus live music in the NICU. They strongly expected that their preferred music could be beneficial both for the infants, parents and staff. The parents agreed most that live music is the most suitable choice for infants (p < 0.001), and that the music could especially benefit the infant (p < 0.001) and parents (p < 0.001) compared with the nurses’ and physicians’ preferences. Some background details such as age, average length of time listening to music, musical training and experiences of using music provided significant explanations for the participants’ expectations. In conclusion, there were significant differences between the groups of the respondents concerning the type of the preferred music and its expected effects. This highlights the importance of discussion with the caregivers when taking music into regular use in the neonatal intensive care units.展开更多
AIM: To evaluate neoangiogenesis in patients with colon cancer by two fluorescently labeled antibodies on fresh biopsy samples imaged with confocal laser endomicroscopy(CLE).METHODS: CLE is an imaging technique for ga...AIM: To evaluate neoangiogenesis in patients with colon cancer by two fluorescently labeled antibodies on fresh biopsy samples imaged with confocal laser endomicroscopy(CLE).METHODS: CLE is an imaging technique for gastrointestinal endoscopy providing in vivo microscopy at subcellular resolution.An important question in validating tumor angiogenesis is what proportion of the tumor vascular network is represented by preexisting parent tissue vessels and newly formed vessels.CD105(endoglin) represents a proliferation-associated endothelial cell adhesion molecule.In contrast to panendothelial markers,such as CD31,CD105 is preferentially expressed in activated endothelial cells that participate in neovascularization.Thus,we evaluated CD105 and CD31 expression from samples of ten patients with primary rectal adenocarcinoma,using a dedicated endomicroscopy system.A imaging software was used to obtain the Z projection of the confocal serial images from each biopsy sample previously combined into stacks.Vascular density and vessel diameters were measured within two 50 μm x 475 μm rectangular regions of interest centered in the middle of each image in the horizontal and vertical direction.The results were averaged over all the patients and were expressed as the mean ± SE.RESULTS: The use of an anti-CD105 antibody was found to be suitable for the detection of blood vessels in colon cancer.Whereas anti-CD31 antibodies stained blood vessels in both normal and pathologic colon equally,CD105 expression was observed primarily in malignant lesions,with little or no expression in the vessels of the normal mucosa(244.21 ± 130.7 vessels/mm3 in only four patients).The average diameter of antiCD105 stained vessels was 10.97 ± 0.6 μm in tumor tissue,and the vessel density was 2787.40 ± 134.8 vessels/mm3.When using the anti-CD31 antibody,the average diameter of vessels in the normal colon tissue was 7.67 ± 0.5 μm and the vessel density was 3191.60 ± 387.8 vessels/mm3,while in the tumors we obtained an average diameter of 10.88 ± 0.8 μm and a vessel density of 4707.30 ± 448.85 vessels/mm3.Thus,there were more vessels stained with CD31 than CD105(P < 0.05).The average vessel diameter was similar for both CD31 and CD105 staining.A qualitative comparison between CLE vs immunohistochemistry lead to similar results.CONCLUSION: Specific imaging and quantification of tumor microvessels are feasible in human rectal cancer using CLE examination and CD105 immunostaining of fresh tissue samples.展开更多
Ambient particulate matter(PM)is the major environmental concern in Tehran,Iran.Exposure to such particles is a serious global health concern and substantial evidence indicates that exposure to particulate matter cont...Ambient particulate matter(PM)is the major environmental concern in Tehran,Iran.Exposure to such particles is a serious global health concern and substantial evidence indicates that exposure to particulate matter contributes to cardiovascular and respiratory disease.Here,we provide a detailed health impact of ambient air pollution in Tehran by linking PM pollution with mortality caused by cardiovascular and respiratory disease.Air quality data of PM obtained from Tehran Air Quality Monitoring Stations(TAQMSs)during 2012–2017.Data mining methods conducted by Spatio-temporal outlier detection and“Air Pollution and Health:a European Approach(APHEA)project”was applied to impute missing values.Time-series data were analyzed to estimate the associations between PM pollution and cause-specific mortality at different lags in Tehran.Based on descriptive analysis,PM_(2.5) is expected to be a major influencing factor in the increased cardiovascular-related death(CVD)and respiratory dysfunction related death(RD)deaths.The cross-correlation analysis showed the same source for PM_(2.5) and PM_(10) in the ambient air of Tehran.Also,the results indicated delays of about 7-15 days from the increased concentrations of PMs and the observed rising in CVD and RD death.Trend analysis showed a more decreasing trend for PM_(2.5) pollutions than PM_(10) pollution and a slightly increasing trend for cause-specific deaths during 2012-2017.Long term exposure to high PM levels increases the CVD and RD deaths and control strategies along with control regulations should be considered to control PM.展开更多
基金Supported by the Romanian National Authority for Scientific Research and Innovation,CNCS-UEFISCDI,project No.PN-IIRU-TE-2014-4-0582,contract No.160/01.10.2015
文摘To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal cancer.METHODSIn this study, we included forty-eight patients with primary colorectal cancer and nine patients for control tissue from the excision of a colonic segment for benign conditions. We determined the clinicopathological features and evaluated the immunohistochemical expression pattern of B2A receptors as well as the morphological changes of the enteric nervous system (ENS). In order to assess statistical differences, we used the student t-test for comparing the means of two groups and one-way analysis of variance with Bonferroni’s post hoc analysis for comparing the means of more than two groups. Correlations were assessed using the Pearson’s correlation coefficient.RESULTSB2A receptors were significantly associated with tumor grading, tumor size, tumor invasion, lymph node metastasis (P < 0.05), while there were no statistically significant associations with gender, CRC location and gross appearance (P > 0.05). We observed, on one hand, a decrease of the relative area for both Auerbach and Meissner plexuses with the increase of the tumor grading, and on the other hand, an increase of the relative area of other nervous elements not in the Meissner plexus or in the Auerbach plexus with the tumor grading. For G1 tumors we found that epithelial B2A area showed an inverse correlation with the Auerbach plexus areas [r(14) = -0.531, P < 0.05], while for G2 tumors, epithelial B2A areas showed an indirect variation with both the Auerbach plexus areas [r(14) = -0.453, P < 0.05] and the Meissner areas [r(14) = -0.825, P < 0.01]. For G3 tumors, the inverse dependence increased for both Auerbach [r(14) = -0.587, P < 0.05] and Meissner [r(14) = -0.934, P < 0.05] plexuses.CONCLUSIONB2A receptors play an important role in colorectal carcinogenesis and can be utilized as prognostic factors. Furthermore, study of the ENS in colorectal cancer may lead to targeted molecular therapies.
文摘Water quality and occurrence of water-borne diseases in the Gaza strip are vivid examples for most developing societies. In recent years, the quality and quantity of groundwater, the only source of waters in the Gaza strip, have deteriorated markedly. A general rundown of the infrastructure and water distribution networks, in particular, the spread of cesspools, excessive use of pesticides and fertilizers, and improper treatment and disposal of wastewater remain major contributing factors to the continued deterioration in the water status in the Gaza strip. Without a doubt, the (Israeli)-Palestinian conflict had a clear negative impact on the water sector in the Gaza strip. Apparently, there is a dire need to adopt the WHO’s water safety plan in the management of Gaza’s water supply systems from catchment to consumer’s tap in order to maintain the sustainability and quality of water resources and prevent outbreaks of waterborne diseases. Therefore, this review has been prepared to highlight the overall picture of the water dilemma in the Gaza strip in the last years and in addition, to identify the sources, sorts, levels, and health risks of consequence to microbial contamination of water. The impact of political conflicts on the water sector in the Gaza strip also was reviewed. Furthermore, recommendations were formulated in order to assist and guide future researchers, stakeholders, and policymakers to avoid the more exacerbation of water contamination as well as to protect public health.
文摘This paper aimed to describe and explain the expectations concerning the use of music in NICU from the viewpoints of parents, nurses and physicians. The relevant questionnaires were distributed to 836 participants who were recruited from the country’s five university hospitals (n = 508, response rate 61). Slightly over half of the participants preferred recorded music versus live music in the NICU. They strongly expected that their preferred music could be beneficial both for the infants, parents and staff. The parents agreed most that live music is the most suitable choice for infants (p < 0.001), and that the music could especially benefit the infant (p < 0.001) and parents (p < 0.001) compared with the nurses’ and physicians’ preferences. Some background details such as age, average length of time listening to music, musical training and experiences of using music provided significant explanations for the participants’ expectations. In conclusion, there were significant differences between the groups of the respondents concerning the type of the preferred music and its expected effects. This highlights the importance of discussion with the caregivers when taking music into regular use in the neonatal intensive care units.
基金Supported by National Research Council(CNCS),Romania,entitled"Clinical and Biomathematical Modeling of Vascular Changes Following anti-angiogenic Therapy in advanced Color-ectal Carcinoma",contract number PN-II-ID-PCE-2011-3-0664the European Social Found,Human Resources Development Operational Programme 2007-2013,No.POSDRU/159/1.5/S/136893
文摘AIM: To evaluate neoangiogenesis in patients with colon cancer by two fluorescently labeled antibodies on fresh biopsy samples imaged with confocal laser endomicroscopy(CLE).METHODS: CLE is an imaging technique for gastrointestinal endoscopy providing in vivo microscopy at subcellular resolution.An important question in validating tumor angiogenesis is what proportion of the tumor vascular network is represented by preexisting parent tissue vessels and newly formed vessels.CD105(endoglin) represents a proliferation-associated endothelial cell adhesion molecule.In contrast to panendothelial markers,such as CD31,CD105 is preferentially expressed in activated endothelial cells that participate in neovascularization.Thus,we evaluated CD105 and CD31 expression from samples of ten patients with primary rectal adenocarcinoma,using a dedicated endomicroscopy system.A imaging software was used to obtain the Z projection of the confocal serial images from each biopsy sample previously combined into stacks.Vascular density and vessel diameters were measured within two 50 μm x 475 μm rectangular regions of interest centered in the middle of each image in the horizontal and vertical direction.The results were averaged over all the patients and were expressed as the mean ± SE.RESULTS: The use of an anti-CD105 antibody was found to be suitable for the detection of blood vessels in colon cancer.Whereas anti-CD31 antibodies stained blood vessels in both normal and pathologic colon equally,CD105 expression was observed primarily in malignant lesions,with little or no expression in the vessels of the normal mucosa(244.21 ± 130.7 vessels/mm3 in only four patients).The average diameter of antiCD105 stained vessels was 10.97 ± 0.6 μm in tumor tissue,and the vessel density was 2787.40 ± 134.8 vessels/mm3.When using the anti-CD31 antibody,the average diameter of vessels in the normal colon tissue was 7.67 ± 0.5 μm and the vessel density was 3191.60 ± 387.8 vessels/mm3,while in the tumors we obtained an average diameter of 10.88 ± 0.8 μm and a vessel density of 4707.30 ± 448.85 vessels/mm3.Thus,there were more vessels stained with CD31 than CD105(P < 0.05).The average vessel diameter was similar for both CD31 and CD105 staining.A qualitative comparison between CLE vs immunohistochemistry lead to similar results.CONCLUSION: Specific imaging and quantification of tumor microvessels are feasible in human rectal cancer using CLE examination and CD105 immunostaining of fresh tissue samples.
文摘Ambient particulate matter(PM)is the major environmental concern in Tehran,Iran.Exposure to such particles is a serious global health concern and substantial evidence indicates that exposure to particulate matter contributes to cardiovascular and respiratory disease.Here,we provide a detailed health impact of ambient air pollution in Tehran by linking PM pollution with mortality caused by cardiovascular and respiratory disease.Air quality data of PM obtained from Tehran Air Quality Monitoring Stations(TAQMSs)during 2012–2017.Data mining methods conducted by Spatio-temporal outlier detection and“Air Pollution and Health:a European Approach(APHEA)project”was applied to impute missing values.Time-series data were analyzed to estimate the associations between PM pollution and cause-specific mortality at different lags in Tehran.Based on descriptive analysis,PM_(2.5) is expected to be a major influencing factor in the increased cardiovascular-related death(CVD)and respiratory dysfunction related death(RD)deaths.The cross-correlation analysis showed the same source for PM_(2.5) and PM_(10) in the ambient air of Tehran.Also,the results indicated delays of about 7-15 days from the increased concentrations of PMs and the observed rising in CVD and RD death.Trend analysis showed a more decreasing trend for PM_(2.5) pollutions than PM_(10) pollution and a slightly increasing trend for cause-specific deaths during 2012-2017.Long term exposure to high PM levels increases the CVD and RD deaths and control strategies along with control regulations should be considered to control PM.
