~ AIM： To study the effects of curcumin on the secretion of interleukin （IL） -6 and IL-8 by corneal limbus epithelial cells. METHODS： Human corneal Iimbus epithelial cells were isolated and cultured from donor ey...~ AIM： To study the effects of curcumin on the secretion of interleukin （IL） -6 and IL-8 by corneal limbus epithelial cells. METHODS： Human corneal Iimbus epithelial cells were isolated and cultured from donor eyes and irradiated by UVB at different dosages with or without curcumin. MTT test was used for studying the effects of UVB and curcumin on the cell viability. The role of mitogen-activated protein kinase （MAPK） and nuclear factor-kappa B （NF-KB） pathways on the UVB-induced secretion of IL.6 and IL-8 were tested by addition of their inhibitors to the culture with or without UVB-radiation. Levels of various signal pathways, IL-6 and IL-8 in the cells and in the conditioned culture medium were measured by ELISA analysis. RESULTS： UVB at 20 mJ/cm2 or less and curcumin at 20 IJmol/L or less did not affect the cell viability of culturedlimbus epithelial cells （P〉0.05）. UVB irradiation at 10 and 20 mJ/cm2 induced a significant increase of secretion of IL-6 and IL-8 and upregulated NF-KB and phosphorylated MAPK pathways of cultured limbus epithelial cells （P〈0.05）. Various signal pathway inhibitors, including SP600125 （JNK inhibitor）, SB203580 （p38 MAPK inhibitor） and BAY11-7082 （NF-KB inhibitor） significantly decreased the UVB-induced secretion of IL-6 and IL-8 secretion （P〈0.05）. Curcumin at 5-20 pmol/L significantly inhibited UVB- induced secretion of IL-6 and IL-8 by limbus epithelial cells in a dose-dependent manner; while curcumin alone did not affect the secretion of IL-6 and IL-8. The upregulation of NF-KB and MAPK pathways induced by UVB treatment was significantly inhibited by curcumin, suggesting that NF-KB and MAPK pathways are involved in the inhibitory effect of curcumin on UVB-induced production of IL-6 and IL-8.展开更多
An estimated 300 million people worldwide suffer from chronic hepatitis C with a prevalence of 0.8%-1.0% of the general population in Canada. An increasing pool of evidence exists supporting the use of pegylated-inter...An estimated 300 million people worldwide suffer from chronic hepatitis C with a prevalence of 0.8%-1.0% of the general population in Canada. An increasing pool of evidence exists supporting the use of pegylated-interferon （pegIFN） and ribavirin combination therapy for hepatitis C. We report a 49-year old male of North American aboriginal descent with chronic hepatitis C （genotype 2b）. Biopsy confirmed that he had cirrhosis with a 2-wk history of left eye pain and decreased visual acuity. He developed retinal vein thrombosis after 16 of 24 wk of pegIFN-α 2a and ribavirin combination therapy. He was urgently referred to a retinal specialist and diagnosed with non-ischemic central retinal vein occlusion of the left eye. PegIFN and ribavirin combination therapy was discontinued and HCV RNA was undetectable after 16 wk of treatment. Hematologic investigations revealed that the patient was a factor V Leiden heterozygote with mildly decreased protein C activity. Our patient had a number of hypercoagulable risk factors, including factor V Leiden heterozygosity, cirrhosis, and hepatitis C that alone would have most likely remained clinically silent. We speculate that in the setting of pegIFN treatment, these risk factors may coalesce and cause the retinal vein thrombosis.展开更多
文摘~ AIM： To study the effects of curcumin on the secretion of interleukin （IL） -6 and IL-8 by corneal limbus epithelial cells. METHODS： Human corneal Iimbus epithelial cells were isolated and cultured from donor eyes and irradiated by UVB at different dosages with or without curcumin. MTT test was used for studying the effects of UVB and curcumin on the cell viability. The role of mitogen-activated protein kinase （MAPK） and nuclear factor-kappa B （NF-KB） pathways on the UVB-induced secretion of IL.6 and IL-8 were tested by addition of their inhibitors to the culture with or without UVB-radiation. Levels of various signal pathways, IL-6 and IL-8 in the cells and in the conditioned culture medium were measured by ELISA analysis. RESULTS： UVB at 20 mJ/cm2 or less and curcumin at 20 IJmol/L or less did not affect the cell viability of culturedlimbus epithelial cells （P〉0.05）. UVB irradiation at 10 and 20 mJ/cm2 induced a significant increase of secretion of IL-6 and IL-8 and upregulated NF-KB and phosphorylated MAPK pathways of cultured limbus epithelial cells （P〈0.05）. Various signal pathway inhibitors, including SP600125 （JNK inhibitor）, SB203580 （p38 MAPK inhibitor） and BAY11-7082 （NF-KB inhibitor） significantly decreased the UVB-induced secretion of IL-6 and IL-8 secretion （P〈0.05）. Curcumin at 5-20 pmol/L significantly inhibited UVB- induced secretion of IL-6 and IL-8 by limbus epithelial cells in a dose-dependent manner; while curcumin alone did not affect the secretion of IL-6 and IL-8. The upregulation of NF-KB and MAPK pathways induced by UVB treatment was significantly inhibited by curcumin, suggesting that NF-KB and MAPK pathways are involved in the inhibitory effect of curcumin on UVB-induced production of IL-6 and IL-8.
文摘An estimated 300 million people worldwide suffer from chronic hepatitis C with a prevalence of 0.8%-1.0% of the general population in Canada. An increasing pool of evidence exists supporting the use of pegylated-interferon （pegIFN） and ribavirin combination therapy for hepatitis C. We report a 49-year old male of North American aboriginal descent with chronic hepatitis C （genotype 2b）. Biopsy confirmed that he had cirrhosis with a 2-wk history of left eye pain and decreased visual acuity. He developed retinal vein thrombosis after 16 of 24 wk of pegIFN-α 2a and ribavirin combination therapy. He was urgently referred to a retinal specialist and diagnosed with non-ischemic central retinal vein occlusion of the left eye. PegIFN and ribavirin combination therapy was discontinued and HCV RNA was undetectable after 16 wk of treatment. Hematologic investigations revealed that the patient was a factor V Leiden heterozygote with mildly decreased protein C activity. Our patient had a number of hypercoagulable risk factors, including factor V Leiden heterozygosity, cirrhosis, and hepatitis C that alone would have most likely remained clinically silent. We speculate that in the setting of pegIFN treatment, these risk factors may coalesce and cause the retinal vein thrombosis.
