OBJECTIVE: To investigate the effects on the brain using three needling manipulations(twirling, lifting-thrusting, and twirling plus lifting-thrusting)when the right-side Zusanli(ST 36) acupoint was stimulated with ne...OBJECTIVE: To investigate the effects on the brain using three needling manipulations(twirling, lifting-thrusting, and twirling plus lifting-thrusting)when the right-side Zusanli(ST 36) acupoint was stimulated with needles.METHODS: Seventeen healthy subjects accepted three needling manipulations stimulating the right Zusanli(ST 36) over separate days. Functional magnetic resonance imaging was used to detect changes in the brain during the manipulations, and then the needling sensations were recorded using the MGH acupuncture sensation scale(MASS) after each scan. f MRI data were processed using Statistical Parametric Mapping 8 to analyze the positiveand negative activation in the brain induced by different acupuncture manipulations.RESULTS: The individual needling sensations showed no statistically significant differences among the three manipulations. However, the MASS index showed that lifting-thrusting > twirling plus lifting-thrusting > twirling. Lifting-thrusting activated left premotor cortex, left postcentral,right middle frontal gyrus, left inferior frontal gyrus,right lingual gyrus, left insula, right putamen, bilateral cingulate gyrus and right cerebellum; and deactivated bilateral hippocampus and left caudate.Twirling activated bilateral orbital middle frontal gyrus, left opercular and triangular inferior frontal gyrus, and right middle occipital gyrus; and deactivated bilateral precuneus, right amygdala, left anterior cingulate gyrus, right inferior temporal gyrus,right middle frontal gyrus, right supplementary motor gyrus, and left postcentral. Twirling plus lifting-thrusting activated bilateral postcentral, left inferior occipital gyrus, left insula, left thalamus, left cingulate gyrus, and right putamen; and deactivated right superior frontal gyrus, right superior parietal gyrus, right temporal gyrus, right middle occipital gyrus, right insula, and left lingual gyrus. Pairwise comparisons of the three manipulations showed that signals induced by lifting-thrusting were the strongest, especially in the limbic system,followed by twirling plus lifting-thrusting; twirling alone was the weakest.CONCLUSION: Three methods of needling manipulations similarly activated areas associated with the somatosensory system, vision, cognition, and emotional regulation. This may have significant implications for acupuncture in clinical practice.展开更多
Myocardial ischemia(MI)causes somatic referred pain and sympathetic hyperactivity,and the role of sensory inputs from referred areas in cardiac function and sympathetic hyperactivity remain unclear.Here,in a rat model...Myocardial ischemia(MI)causes somatic referred pain and sympathetic hyperactivity,and the role of sensory inputs from referred areas in cardiac function and sympathetic hyperactivity remain unclear.Here,in a rat model,we showed that MI not only led to referred mechanical hypersensitivity on the forelimbs and upper back,but also elicited sympathetic sprouting in the skin of the referred area and C8–T6 dorsal root ganglia,and increased cardiac sympathetic tone,indicating sympathetic-sensory coupling.Moreover,intensifying referred hyperalgesic inputs with noxious mechanical,thermal,and electro-stimulation(ES)of the forearm augmented sympathetic hyperactivity and regulated cardiac function,whereas deafferentation of the left brachial plexus diminished sympathoexcitation.Intradermal injection of the α_(2) adrenoceptor(α_(2)AR)antagonist yohimbine and agonist dexmedetomidine in the forearm attenuated the cardiac adjustment by ES.Overall,these findings suggest that sensory inputs from the referred pain area contribute to cardiac functional adjustment via peripheral α_(2)AR-mediated sympathetic-sensory coupling.展开更多
Objective:To investigate the effects of different acupuncture manipulations on Deqi sensations and surface myoelectricity,and explore the correlation between Deqi sensations and needling manipulations.Methods:Forty-fi...Objective:To investigate the effects of different acupuncture manipulations on Deqi sensations and surface myoelectricity,and explore the correlation between Deqi sensations and needling manipulations.Methods:Forty-five healthy participants accepted twirling,lifting-thrusting,and twirling plus lifting-thrusting manipulanions at right Zusanli(ST 36),respectively.The acupuncturist’s and participants’Deqi sensations were collected by MGH Acupuncture Sensation Scale(MASS).The intensity and occurrence rate of soreness,dull pain,pressure,heaviness,fullness,numbness,sharp pain,warmth,coolness,and throbbing feelings of participants,and tightness,smooth,and tangle feelings of acupuncturist were measured.The correlation between the acupuncturist’s and participant’s Deqi sensations was analyzed.Surface electromyogram(EMG)was recorded before,during and after needling in 30 participants.The integrated EMG(iEMG),mean power frequency(MPF)and media frequency(MF)were analyzed.Result:Both fullness and soreness of participants and tightness of acupuncturist were the most frequently occurred ones.A positive correlation between participants’fullness and acupuncturist’s tightness was observed during the three aforementioned needling manipulations(P<0.05,OR>1).Almost all the needling sensations measured in the present study could be induced by the three needling manipulations.However,strength of Deqi sensations was exhibited as lifting-thrusting>twirling plus lifting-thrusting>twirling according to MASS index.The i EMG values were increased and MPF,MF values were decreased during needling compaired to those before needling,especially during lifting-thrusting(P<0.01).Conclusions:The intensity and occurrence rate of the different Deqi sensations induced by different needling manipulations were basically similar.The fullness and soreness were both the most frequently induced Deqi sensations.The strongest Deqi sensation could be induced by lifting-thrusting manipulation.There is a positive correlation between participants’fullness and acupuncturist’s tightness during the three needling manipulations.The myoelectricity around the acupoint is related to Deqi responses.(Registration No.AMCTR-IOR-20000314).展开更多
基金Supported by China Academy of Chinese Medical Sciences Foundation(No.ZZ08010)General Program of National Natural Science Foundation of China(No.81273674)
文摘OBJECTIVE: To investigate the effects on the brain using three needling manipulations(twirling, lifting-thrusting, and twirling plus lifting-thrusting)when the right-side Zusanli(ST 36) acupoint was stimulated with needles.METHODS: Seventeen healthy subjects accepted three needling manipulations stimulating the right Zusanli(ST 36) over separate days. Functional magnetic resonance imaging was used to detect changes in the brain during the manipulations, and then the needling sensations were recorded using the MGH acupuncture sensation scale(MASS) after each scan. f MRI data were processed using Statistical Parametric Mapping 8 to analyze the positiveand negative activation in the brain induced by different acupuncture manipulations.RESULTS: The individual needling sensations showed no statistically significant differences among the three manipulations. However, the MASS index showed that lifting-thrusting > twirling plus lifting-thrusting > twirling. Lifting-thrusting activated left premotor cortex, left postcentral,right middle frontal gyrus, left inferior frontal gyrus,right lingual gyrus, left insula, right putamen, bilateral cingulate gyrus and right cerebellum; and deactivated bilateral hippocampus and left caudate.Twirling activated bilateral orbital middle frontal gyrus, left opercular and triangular inferior frontal gyrus, and right middle occipital gyrus; and deactivated bilateral precuneus, right amygdala, left anterior cingulate gyrus, right inferior temporal gyrus,right middle frontal gyrus, right supplementary motor gyrus, and left postcentral. Twirling plus lifting-thrusting activated bilateral postcentral, left inferior occipital gyrus, left insula, left thalamus, left cingulate gyrus, and right putamen; and deactivated right superior frontal gyrus, right superior parietal gyrus, right temporal gyrus, right middle occipital gyrus, right insula, and left lingual gyrus. Pairwise comparisons of the three manipulations showed that signals induced by lifting-thrusting were the strongest, especially in the limbic system,followed by twirling plus lifting-thrusting; twirling alone was the weakest.CONCLUSION: Three methods of needling manipulations similarly activated areas associated with the somatosensory system, vision, cognition, and emotional regulation. This may have significant implications for acupuncture in clinical practice.
基金supported by the National Key R&D Program of China(2018YFC1704600)the National Natural Science Foundation of China(81674085,81904309).
文摘Myocardial ischemia(MI)causes somatic referred pain and sympathetic hyperactivity,and the role of sensory inputs from referred areas in cardiac function and sympathetic hyperactivity remain unclear.Here,in a rat model,we showed that MI not only led to referred mechanical hypersensitivity on the forelimbs and upper back,but also elicited sympathetic sprouting in the skin of the referred area and C8–T6 dorsal root ganglia,and increased cardiac sympathetic tone,indicating sympathetic-sensory coupling.Moreover,intensifying referred hyperalgesic inputs with noxious mechanical,thermal,and electro-stimulation(ES)of the forearm augmented sympathetic hyperactivity and regulated cardiac function,whereas deafferentation of the left brachial plexus diminished sympathoexcitation.Intradermal injection of the α_(2) adrenoceptor(α_(2)AR)antagonist yohimbine and agonist dexmedetomidine in the forearm attenuated the cardiac adjustment by ES.Overall,these findings suggest that sensory inputs from the referred pain area contribute to cardiac functional adjustment via peripheral α_(2)AR-mediated sympathetic-sensory coupling.
基金Supported by the National Natural Science Foundation of China(No.81973758)China Academy of Chinese Medical Sciences Foundation(Nos.ZZ11-048,ZZ-ZR2017004,and ZZ08010)。
文摘Objective:To investigate the effects of different acupuncture manipulations on Deqi sensations and surface myoelectricity,and explore the correlation between Deqi sensations and needling manipulations.Methods:Forty-five healthy participants accepted twirling,lifting-thrusting,and twirling plus lifting-thrusting manipulanions at right Zusanli(ST 36),respectively.The acupuncturist’s and participants’Deqi sensations were collected by MGH Acupuncture Sensation Scale(MASS).The intensity and occurrence rate of soreness,dull pain,pressure,heaviness,fullness,numbness,sharp pain,warmth,coolness,and throbbing feelings of participants,and tightness,smooth,and tangle feelings of acupuncturist were measured.The correlation between the acupuncturist’s and participant’s Deqi sensations was analyzed.Surface electromyogram(EMG)was recorded before,during and after needling in 30 participants.The integrated EMG(iEMG),mean power frequency(MPF)and media frequency(MF)were analyzed.Result:Both fullness and soreness of participants and tightness of acupuncturist were the most frequently occurred ones.A positive correlation between participants’fullness and acupuncturist’s tightness was observed during the three aforementioned needling manipulations(P<0.05,OR>1).Almost all the needling sensations measured in the present study could be induced by the three needling manipulations.However,strength of Deqi sensations was exhibited as lifting-thrusting>twirling plus lifting-thrusting>twirling according to MASS index.The i EMG values were increased and MPF,MF values were decreased during needling compaired to those before needling,especially during lifting-thrusting(P<0.01).Conclusions:The intensity and occurrence rate of the different Deqi sensations induced by different needling manipulations were basically similar.The fullness and soreness were both the most frequently induced Deqi sensations.The strongest Deqi sensation could be induced by lifting-thrusting manipulation.There is a positive correlation between participants’fullness and acupuncturist’s tightness during the three needling manipulations.The myoelectricity around the acupoint is related to Deqi responses.(Registration No.AMCTR-IOR-20000314).
