In order to cultivate excellent clinical medical talents with a solid foundation, strong literacy, refined skills, excellent communication abilities, innovative thinking, and a strong focus on practicality, functional...In order to cultivate excellent clinical medical talents with a solid foundation, strong literacy, refined skills, excellent communication abilities, innovative thinking, and a strong focus on practicality, functional experiments have undergone a series of reforms in areas such as constructing new curriculum systems, improving teaching content, updating teaching equipment, introducing new teaching models, and enhancing teaching evaluation systems.展开更多
A rapid and sensitive liquid chromatography-tandem mass chromatography (LC-MS/MS) method has been developed and validated for simultaneous determination of catalpol and harpagide in rat plasma. The samples were extrac...A rapid and sensitive liquid chromatography-tandem mass chromatography (LC-MS/MS) method has been developed and validated for simultaneous determination of catalpol and harpagide in rat plasma. The samples were extracted by one-step protein precipitation and separated on a SunFireTM C18 column (100 mm × 2.1 mm, 3.5 μm;Waters) using acetonitrile-10 mM ammonium formate as mobile phase at a flow rate 0.3 mL/min in gradient mode. The analytes were detected without interference in Multiple Reaction Monitoring (MRM) mode with negative electrospray ionization. Linear responses were obtained for catalpol ranging from 20 to 5000 ng/mL and harpagide ranging from 10 to 2500 ng/mL. Coefficients of correlation (r) for the calibration curves were more than 0.99 for both analytes. Intra- and inter-day accuracy and precision were within the acceptable limits of less than 15.0% at all concentrations. The quantitation method was successfully applied for simultaneous estimation of catalpol and harpagide after oral administration of Zeng Ye Decoction.展开更多
Alzheimer’s disease (AD) is the most common form of dementia which mostly affects persons younger than 65 years old. Mounting findings showed that amyloid-β(Aβ) peptides, oxidative stress, neuroinfl...Alzheimer’s disease (AD) is the most common form of dementia which mostly affects persons younger than 65 years old. Mounting findings showed that amyloid-β(Aβ) peptides, oxidative stress, neuroinflammation and insulin resistance may play central role in the pathogenesis of AD. There are very many methods to slow it through affecting these aforementioned factors. However, more efficient prevention of the progression of AD is still ambiguous. Fibroblast growth factor 21 (FGF21) is an endocrine hormone that is expressed by several organs. It increases insulin sensitivity and regulates lipid metabolism and energy homeostasis. Emerging evidence demonstrates that FGF21 has potential effects in the brain involving metabolic regulation, neuroprotection and cognition. Hence, we hypothesize that FGF21 may be a protective factor in AD by attenuating Aβ generation, inflammation, oxidative stress, and insulin resistance. Our hypothesis will shed new light on the understanding of pathogenesis of AD and help to find a new way to prevent the genesis and progress of AD.展开更多
Purpose: To analyze the miRNA expression profiles in C57 mice ageing hippocampus in detail, and investigate the functional information of these hippocampus specific miRNAs and the related regulatory networks. Methods:...Purpose: To analyze the miRNA expression profiles in C57 mice ageing hippocampus in detail, and investigate the functional information of these hippocampus specific miRNAs and the related regulatory networks. Methods: Microarrays were used to analyze miRNA expression profiles in adult and aged hippocampi, and bioinformatics analysis methods, such as three public datasets (Mirbase, Miranda, and Mirdb), DAVID online tools and KEGG pathway tools were used to study in detail the target genes of the differentially expressed miRNAs. Results: 26 differentially expressed miRNAs were identified by greater than 1.5-fold change (intersection of two sets), of which 16 were up-regulated and 10 were down-regulated. DAVID Functional Annotation Cluster (FAC) analysis of the 132 predicted target genes of up-regulated miRNAs revealed confident enrichment scores for synaptic function and apoptosis etc. (Figure 1), indicating the functional significance and importance of these miRNAs during hippocampal ageing. Conclusions: Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus ageing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in ageing process of brain tissue.展开更多
MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocamp...MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocampal development, in this study, newborn and adult hippocampi were deliberately selected to analyze the miRNA expression profiles by microarray. Microarray analyses identified 22 differentially expressed miRNAs (>1.5 fold, intersection of two sets). Of these, 12 were down-regulated and 10 were up-regulated during hippocampus development. DAVID Functional Annotation Cluster (FAC) analysis of the 317 predicted target genes of down-regulated miRNAs revealed confident enrichment scores for cell adhesion and neuron development etc., indicating the functional significance and importance of these miRNAs during hippocampal development. Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus developing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in brain tissue.展开更多
MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays ...MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays a crucial role in learning and memory. In this study, we detected the negative relationship between CREB1 protein and miR-181d expression in lewis rat hippocampus development. And the bioinformatic analysis showed that, CREB1 mRNA contains complementary sequence to the miR-181d seed region. Then we further demonstrated that miR-181d controls the expression level of CREB1 gene in PC12 cells by luciferase assay and western blot. Taken together, our data demonstrated that CREB1 mRNA is the target gene of miR-181d, and conformed CREB1 protein expression was regulated by miR-181d in PC12 cells.展开更多
文摘In order to cultivate excellent clinical medical talents with a solid foundation, strong literacy, refined skills, excellent communication abilities, innovative thinking, and a strong focus on practicality, functional experiments have undergone a series of reforms in areas such as constructing new curriculum systems, improving teaching content, updating teaching equipment, introducing new teaching models, and enhancing teaching evaluation systems.
文摘A rapid and sensitive liquid chromatography-tandem mass chromatography (LC-MS/MS) method has been developed and validated for simultaneous determination of catalpol and harpagide in rat plasma. The samples were extracted by one-step protein precipitation and separated on a SunFireTM C18 column (100 mm × 2.1 mm, 3.5 μm;Waters) using acetonitrile-10 mM ammonium formate as mobile phase at a flow rate 0.3 mL/min in gradient mode. The analytes were detected without interference in Multiple Reaction Monitoring (MRM) mode with negative electrospray ionization. Linear responses were obtained for catalpol ranging from 20 to 5000 ng/mL and harpagide ranging from 10 to 2500 ng/mL. Coefficients of correlation (r) for the calibration curves were more than 0.99 for both analytes. Intra- and inter-day accuracy and precision were within the acceptable limits of less than 15.0% at all concentrations. The quantitation method was successfully applied for simultaneous estimation of catalpol and harpagide after oral administration of Zeng Ye Decoction.
文摘Alzheimer’s disease (AD) is the most common form of dementia which mostly affects persons younger than 65 years old. Mounting findings showed that amyloid-β(Aβ) peptides, oxidative stress, neuroinflammation and insulin resistance may play central role in the pathogenesis of AD. There are very many methods to slow it through affecting these aforementioned factors. However, more efficient prevention of the progression of AD is still ambiguous. Fibroblast growth factor 21 (FGF21) is an endocrine hormone that is expressed by several organs. It increases insulin sensitivity and regulates lipid metabolism and energy homeostasis. Emerging evidence demonstrates that FGF21 has potential effects in the brain involving metabolic regulation, neuroprotection and cognition. Hence, we hypothesize that FGF21 may be a protective factor in AD by attenuating Aβ generation, inflammation, oxidative stress, and insulin resistance. Our hypothesis will shed new light on the understanding of pathogenesis of AD and help to find a new way to prevent the genesis and progress of AD.
文摘Purpose: To analyze the miRNA expression profiles in C57 mice ageing hippocampus in detail, and investigate the functional information of these hippocampus specific miRNAs and the related regulatory networks. Methods: Microarrays were used to analyze miRNA expression profiles in adult and aged hippocampi, and bioinformatics analysis methods, such as three public datasets (Mirbase, Miranda, and Mirdb), DAVID online tools and KEGG pathway tools were used to study in detail the target genes of the differentially expressed miRNAs. Results: 26 differentially expressed miRNAs were identified by greater than 1.5-fold change (intersection of two sets), of which 16 were up-regulated and 10 were down-regulated. DAVID Functional Annotation Cluster (FAC) analysis of the 132 predicted target genes of up-regulated miRNAs revealed confident enrichment scores for synaptic function and apoptosis etc. (Figure 1), indicating the functional significance and importance of these miRNAs during hippocampal ageing. Conclusions: Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus ageing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in ageing process of brain tissue.
文摘MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocampal development, in this study, newborn and adult hippocampi were deliberately selected to analyze the miRNA expression profiles by microarray. Microarray analyses identified 22 differentially expressed miRNAs (>1.5 fold, intersection of two sets). Of these, 12 were down-regulated and 10 were up-regulated during hippocampus development. DAVID Functional Annotation Cluster (FAC) analysis of the 317 predicted target genes of down-regulated miRNAs revealed confident enrichment scores for cell adhesion and neuron development etc., indicating the functional significance and importance of these miRNAs during hippocampal development. Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus developing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in brain tissue.
文摘MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays a crucial role in learning and memory. In this study, we detected the negative relationship between CREB1 protein and miR-181d expression in lewis rat hippocampus development. And the bioinformatic analysis showed that, CREB1 mRNA contains complementary sequence to the miR-181d seed region. Then we further demonstrated that miR-181d controls the expression level of CREB1 gene in PC12 cells by luciferase assay and western blot. Taken together, our data demonstrated that CREB1 mRNA is the target gene of miR-181d, and conformed CREB1 protein expression was regulated by miR-181d in PC12 cells.
