This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of...This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of highly skilled talents in urban agriculture in the era of smart agriculture and their cultivation pathways and practices.It proposes measures such as building multi-level practical teaching platforms,implementing the“Enjoy Horticulture”series of high-quality activities,and establishing the“1234”applied talent training model to cultivate high-quality talents that meet the development needs of modern urban horticulture industry.Taking Beijing University of Agriculture and other universities as examples,the paper analyzes the practical cases and effects of the urban horticulture discipline’s industry-education-research collaborative talent training model,which has reference significance for further improving and perfecting the urban horticulture industry-education-research collaborative talent training plan.展开更多
Lignin is a significant secondary metabolite produced through the phenylpropanoid pathway.As a vital component of the plant cell wall,lignin affects various fruit characteristics,including size,seed quantity,and firmn...Lignin is a significant secondary metabolite produced through the phenylpropanoid pathway.As a vital component of the plant cell wall,lignin affects various fruit characteristics,including size,seed quantity,and firmness.In this study,we conducted comprehensive identification and phylogenetic analysis of 265 Caffeic acid O-methyltransferase(COMT)genes across ten different plant species,including Vaccinium corymbosum and four other Vaccinium species.The results reveal that VcCOMT38 is a promising structural gene for the biosynthesis of lignin in blueberry.An in vitro enzymatic assay of VcCOMT38 demonstrated that it is a special enzyme in the lignin biosynthesis pathway and prefers to use caffeic acid as a substrate over 5-hydroxyferulic acid.Transient overexpression and silencing of VcCOMT38 in Vaccinium corymbosum‘Northland’fruits demonstrated that VcCOMT38 participates in lignin biosynthesis and contributes to both an increased number of immature seeds and enhanced fruit firmness.The heterologous overexpression of VcCOMT38 in Nicotiana benthamiana revealed that this gene could increase the lignin content and the syringyl/guaiacyl(S/G)ratio,which determines the maximum monomer yield during lignin depolymerization.These results highlight VcCOMT38 as a crucial gene in lignin biosynthesis and its potential for improving lignin production in industry through genetically modified woody plants.展开更多
Directed breeding of horticultural crops is essential for increasing yield,nutritional content,and consumer-valued characteristics such as shape and color of the produce.However,limited genetic diversity restricts the...Directed breeding of horticultural crops is essential for increasing yield,nutritional content,and consumer-valued characteristics such as shape and color of the produce.However,limited genetic diversity restricts the amount of crop improvement that can be achieved through conventional breeding approaches.Natural genetic changes in cisregulatory regions of genes play important roles in shaping phenotypic diversity by altering their expression.Utilization of CRISPR/Cas editing in crop species can accelerate crop improvement through the introduction of genetic variation in a targeted manner.The advent of CRISPR/Cas-mediated cis-regulatory region engineering(cis-engineering)provides a more refined method for modulating gene expression and creating phenotypic diversity to benefit crop improvement.Here,we focus on the current applications of CRISPR/Cas-mediated cis-engineering in horticultural crops.We describe strategies and limitations for its use in crop improvement,including de novo cis-regulatory element(CRE)discovery,precise genome editing,and transgene-free genome editing.In addition,we discuss the challenges and prospects regarding current technologies and achievements.CRISPR/Cas-mediated cis-engineering is a critical tool for generating horticultural crops that are better able to adapt to climate change and providing food for an increasing world population.展开更多
Organic matter increases biological activity within the root zone because it contains beneficial microbes that stimulate vital processes.This study aimed to determine the effect of Ochrobactrum anthropi added to the s...Organic matter increases biological activity within the root zone because it contains beneficial microbes that stimulate vital processes.This study aimed to determine the effect of Ochrobactrum anthropi added to the soil as a single or mixed solution with different concentrations of organic matter on the vegetative growth of the zinnia plant and its content of nutrients.The study was conducted with a randomized complete block design(RCBD)with three replications.The plant was grown in soil with a high salinity level of 8 ds m^(–1).Plant characteristics were estimated 30 and 60 days after planting the plant.The treatment with the addition of bio-inoculum produced the best results;within 30 days,there was a 56.89%increase in plant length;after 60 days,there was a 52.56%increase;additionally,there was a 52.56%increase in leaf count within 30 days;after 60 days,there was a 53.50%increase;and finally,there was an increase in flower count.For plants after 60 days,it reached 3.66%.With the addition of bio-inoculum to soil at a level of 3 gm kg^(–1)of organic matter,the mixing treatment achieved the highest dry weight,29.86%.The addition of bio-inoculum resulted in the largest and most significant increase in chlorophyll content in leaves,reaching 18.76%.In the mixing addition of the biological inoculum treatment,the organic content of the plant showed an increase in nitrogen(14.38%),phosphorus(21.18%),and potassium(39.75%)at 2 and 3 gm kg^(–1)organic matter,respectively.展开更多
Pepper (Capsicum annuum L.) is an important agricultural crop because of the nutritional value of the fruit and its economic importance.Various techniques have been practiced to enhance pepper's productivity and n...Pepper (Capsicum annuum L.) is an important agricultural crop because of the nutritional value of the fruit and its economic importance.Various techniques have been practiced to enhance pepper's productivity and nutritional value.Therefore,this study was conducted to determine the impact of different training methods and biostimulant applications on sweet pepper plants'growth,yield,and chemical composition under greenhouse conditions.For the training method,unpruned plants were compared with one stem and two stem plants.Unpruned plants had the fruit number of 33.98,fruit weight of 2.18 kg·plant^(-1),and total marketable yield of 1 090.0 kg·hm^(-2).One stem plant gave the best average fruit weight of 86.63 g,vitamin C content of 13.66 mg·kg^(-1)FW,and TSS content of 7.21%.However,two stem plants had the highest fruit setting of 62.41%,carotenoid content of 0.14 mg·kg^(-1)FW,and fruit chlorophyll content of 3.57 mg·kg^(-1)FW.For biostimulant applications,control plants were compared with the Disper Root (DR) and Disper Vital (DV).DR application significantly increased total sugar,carotenoid,fruit chlorophyll,and TSS contents compared to the control and DV applications.While,applying DV increased fruit setting,plant fruit number,weight,and total marketable yield.In addition,integrating one stem plant with the DR application improved fiber,vitamin C,and TSS contents significantly.Two stem plants,and the DV application improved fruit setting and carotenoid content.Thus,one and two stem training methods integrated with the DR and DV biostimulant applications could be considered for developing agricultural practices to obtain commercial yield and improve the nutrition values of sweet peppers,as unpruned plants without biostimulant applications have a negative impact.展开更多
The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for se...The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for seedling production in nursery conditions often remain below the expected productivity level due to biotic and abiotic stress-related reasons.Studies to increase nursery yields are still on the agenda of grapevine researchers.In this study,the effects of silver nanoparticles(AgNPs)produced by the green synthesis method using grape seed extract and AgNO3 on rooting and vegetative growth of the standard(TS 4027)cuttings taken during the dormancy period of Vitis vinifera L.cvs Ekşi Kara and GökÜzüm were investigated under greenhouse conditions.Cuttings treated by keeping in 0.1,0.2 g·L^(−1)AgNPs,0.1,0.2 g·L^(−1)IBA aqueous solutions for 24 h were planted in black,1 L volume seedling bags filled with 1:1 peat:perlite in the greenhouse,while the control was kept in pure water for 24 h and planted.Changes in sprouting rate,plant transformation rate,shoot length,shoot diameter,number of nodes,stomatal conductance,leaf temperature,photosynthetic efficiency,leaf fresh and dry weight,SPAD,root number,root length,root fresh and root weight were examined in developing seedlings.In evaluating the effects of AgNPs and Indole-3-butyric acid(IBA)treatments on cutting rooting and vegetative development,ANOVA,post hoc analysis with the Tukey test,and Principal Component Analyses(PCAs)were used to better understand and depict the correlations between the examined variables.This analysis method was performed using ggplot2 in the R Studio program.The heatmap generated by the pheatmap package was used to visualize the correlation and variation.As a result of this study,AgNPs applications were found to be more effective than IBA treatments in the rooting of grapevine cuttings and the vegetative development of young plants.In conclusion,0.1 g·L^(−1)AgNPs can be tested as a support and/or economical alternative to IBA for the promotion of rooting of cuttings and vegetative development of young plants for subsequent clonal propagation.展开更多
Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation s...Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation strategies are widely recommended to enhance water use efficiency,knowledge remains limited regarding their interactions with soil amendments such as biochar and the consequent impacts on reproductive traits.This study aimed to evaluate the combined effects of deficit irrigation strategies and biochar application on pollen viability and morphology in Capsicum annuum.The experiment was conducted under full,partial,and deficit irrigation regimes with and without biochar treatment,following a randomized block design.The primary parameters examined were pollen viability(viable,semi-viable,and non-viable rates),anther width and length,and pollen width and length.Microscopic measurements and statistical analyses(p≤0.05)revealed significant effects of both irrigation regimes and biochar applications.Under deficit irrigation,viable,semi-viable,and non-viable pollen rates were 29.84%,32.95%,and 37.21%,respectively,whereas the highest viable pollen rate was observed under full irrigation.In partial irrigation,viable pollen accounted for 31.67%,semi-viable for 38.81%,and non-viable for 29.49%.In plots treated with biochar under partial irrigation,anther width(1700.89μm),anther length(3805.34μm),pollen width(26.93μm),and pollen length(37.42μm)reached the highest values,while the lowest values were recorded in deficit irrigation plots without biochar.These findings emphasize the importance of integrating biochar into irrigation management to mitigate the adverse effects of water stress on pollen development.Nevertheless,further research is needed to clarify the long-term implications of these practices for reproductive success and agricultural sustainability.展开更多
Bemisia tabaci is a polyphagous herbivore that feeds on a wide range of horticultural and ornamental crops cultivated under diverse ecological zones. In Sierra Leone, B. tabaci is found to infest a wide range of veget...Bemisia tabaci is a polyphagous herbivore that feeds on a wide range of horticultural and ornamental crops cultivated under diverse ecological zones. In Sierra Leone, B. tabaci is found to infest a wide range of vegetable crops by directly feeding on phloem sap thereby inducing physiological disorders, and also serve as a vector to gemini viruses. Invariably the destructive feeding of B. tabaci affects the productivity and aesthetic values of vegetables and other horticultural crops and hence is considered a serious economic pest. A bioassay experiment was carried out by rearing B. tabaci populations on four vegetable crops under controlled laboratory conditions to determine its life table and demographic parameters. Results showed that the intrinsic rate of growth which measures the population size and growth pattern was highest for populations reared on tomato crops with the following values: rm 0.145 female female−1 day−1, the gross reproduction rate (Ro), and finite growth rate λ were highest for population reared on tomato, correspondingly the development period from egg-adult emergence was shortest with a value of 26 d. Conversely, the computed demographical parameters rm, λ and Ro for the population reared on sweet pepper were 0.106 female female−1 day−1 respectively, with a corresponding development period egg-adult emergence as 36d. The computed biological parameters for okra and garden egg varied with intermediary values between tomato and pepper host materials. The survivorship rates were quite significant for the smaller instars (Instars 1-III) with over 80% surviving to pre-pupa and pupa stage for the populations reared for all the test materials. High mortality was noticed for the pre-pupa and pupa stages as their survival rates were significantly low compared to the high survival rates of the smaller instars. Less than 50% of pupae failed to emerge to adults except for populations reared on tomato test materials where 52% emerged to adults. The study indicated tomato as the most suitable host among the four vegetable crops. Although life table and demographic parameters are invaluable information for forecasting pest populations and help in designing pest management efforts, further investigations such as the economic threshold and economic injury levels of B. tabaci population are requisite decision tools for sound pest management decisions of B. tabaci on these vegetable crops. The information obtained from this investigation would be quite relevant for extension service and pest management practitioners where mixed vegetable farming is a common practice.展开更多
Newly planted apple orchards in the USA comprising the highly biennial cultivar, “Honeycrisp”, are prone to flower within the first two years from planting and set fruit. These processes limit canopy development and...Newly planted apple orchards in the USA comprising the highly biennial cultivar, “Honeycrisp”, are prone to flower within the first two years from planting and set fruit. These processes limit canopy development and subsequent yield potential. GA4 + 7 can inhibit floral formation processes of apples. The timing and dose for eliminating return bloom of young “Honeycrisp” trees, however, is unclear. A factorial experimental design to test GA4 + 7 application timing and rate produced significant reductions in return bloom for both factors and their interaction. Treatment responses demonstrated that florigenic processes in “Honeycrisp” occurred early. The most pronounced reduction in return bloom followed the 2-week after full bloom (WAFB) application timing, increasing with increasing rate. The effect on return bloom was progressively diminished over the next two weeks of applications but remained significantly lower than the control. Three successive applications timed one week apart eliminated return bloom of spurs at the highest rate. Both factors and their interaction also significantly reduced return bloom of terminal buds of leaders;in contrast to floral buds on spurs, the response of terminal buds on leaders improved with delayed application timings. Timing exerted a stronger effect than rate on return flowering of the terminal buds of leaders. Leader growth was positively affected by GA4 + 7, the year of application, when three successive applications were made. Tree height, overall growth, expressed as the increase in trunk cross-sectional area, and limb number were also significantly improved by GA4 + 7 but varied in their responses to application timing and rate, and were inconsistent. Overall, successive applications of GA4 + 7 had good efficacy for inhibiting floral initiation of meristems on spurs and terminals while improving the vegetative growth of young “Honeycrisp” trees.展开更多
Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives wer...Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.展开更多
Phalaenopsis orchids are economically important ornamental crops;however,their commercial micropropagation is often limited by poor rooting efficiency and inconsistent growth.In this study,we investigated the effects ...Phalaenopsis orchids are economically important ornamental crops;however,their commercial micropropagation is often limited by poor rooting efficiency and inconsistent growth.In this study,we investigated the effects of silver nanoparticles(Ag-NPs)on the in vitro regeneration and growth of Phalaenopsis cultivar 611B to determine the optimal concentration of Ag-NPs for improved micropropagation outcomes.Shoot tip explants(2–3 mm)—derived from protocorm-like bodies were cultured on a regeneration medium containing Hyponex(20:20:20 and 6.5:6.5:19),18 g/L sugar,2 g/L peptone,0.8 g/L activated charcoal,12.5 g/L potato extract,50 mL/L apple juice,and 10 mg/L 6-benzylaminopurine(6-BA),with varying concentrations of Ag-NPs(0,0.5,1.0,2.0,and 2.5 mg/L).After 10–12 weeks,shoot and root formation,plant height,fresh weight,leaf number,and chlorophyll contents were evaluated.At 1.0 mg/L Ag-NPs,shoot regeneration(5.4 vs.2.9 shoots per explant),root induction(2.1 vs.1.4 roots per explant),and shoot formation frequency(100%vs.55%)were significantly higher than the control(0 mg/L).Fresh weight(592.4 mg)and leaf number(9.7)also showed notable increases at this concentration.Although chlorophyll a and b levels peaked at 2.0 mg/L,the difference from 1.0 mg/L was not statistically significant.These results suggest that 1.0mg/LAg-NPs is the optimal concentration for enhancing shoot and root development and improving overall plantlet quality in Phalaenopsis.The findings highlight the potential of nanomaterials to improve the efficiency of orchid tissue culture systems.展开更多
This study was conducted to determine the content,distribution and transformation of iron oxides in the soils of the Middle Euphrates regions in Iraq.The study included four sites:Tuwairij area in Karbala Governorate,...This study was conducted to determine the content,distribution and transformation of iron oxides in the soils of the Middle Euphrates regions in Iraq.The study included four sites:Tuwairij area in Karbala Governorate,College of Agriculture at the University of Kufa in Najaf Governorate,College of Agriculture at the University of Qadisiyah in Diwaniyah Governorate,and the Nile District in Babylon Governorate.The results showed that the soils of Najaf and Qadisiyah were superior in terms of their content of total free iron oxides(Fet)compared to the soils of Karbala and Babylon.The relative distribution of free iron oxides was generally close among the studied sites,with a homogeneous pattern in the distribution of these oxides within the soil horizons.As for silicate iron oxides(Fes),a homogeneous pattern was observed in the soil of Babylon with its content increasing with depth,while these patterns varied in the soils of Karbala,Najaf and Qadisiyah.Regarding the ratios of crystalline iron oxides(Fed/Fet),the study showed that the Babylon and Qadisiyah soils recorded the highest values,while these values were lower in the Najaf and Karbala soils.On the other hand,amorphous iron oxides(FeO)showed similar values in the Najaf and Qadisiyah soils.In general,these results clearly showed the effect of environmental and geochemical factors of the study areas on the distribution and transformations of iron oxides in the soil of the Middle Euphrates regions.展开更多
Plant height(PH),primary lateral branch length(PBL),and branch number(BN)are architectural components impacting peanut pod yield,biomass production,and adaptivity to mechanical harvesting.In this study,a recombinant i...Plant height(PH),primary lateral branch length(PBL),and branch number(BN)are architectural components impacting peanut pod yield,biomass production,and adaptivity to mechanical harvesting.In this study,a recombinant inbred population consisting of 181 individual lines was used to determine genetic controls of PH,PBL,and BN across three environments.Phenotypic data collected from the population demonstrated continuous distributions and transgressive segregation patterns.Broad-sense heritability of PH,PBL,and BN was found to be 0.87,0.88,and 0.92,respectively.Unconditional individual environmental analysis revealed 35 additive QTLs with phenotypic variation explained(PVE)ranging from 4.57 to 21.68%.A two-round meta-analysis resulted in 24consensus and 19 unique QTLs.Five unique QTLs exhibited pleiotropic effects and their genetic bases(pleiotropy or tight linkage)were evaluated.A joint analysis was performed to estimate the QTL by environment interaction(QEI)effects on PH,PBL,and BN,collectively explaining phenotypic variations of 10.80,11.02,and 7.89%,respectively.We identified 3 major and stable QTL regions(uq9-3,uq10-2,and uq16-1)on chromosomes 9,10,and 16,spanning1.43-1.53 Mb genomic regions.Candidate genes involved in phytohormones biosynthesis,signaling,and cell wall development were proposed to regulate these morphological traits.These results provide valuable information for further genetic studies and the development of molecular markers applicable to peanut architecture improvement.展开更多
Plants are continuously exposed to abiotic and biotic stresses that threaten their growth,reproduction,and survival.Adaptation to these stresses requires complex regulatory networks that coordinate physiological,molec...Plants are continuously exposed to abiotic and biotic stresses that threaten their growth,reproduction,and survival.Adaptation to these stresses requires complex regulatory networks that coordinate physiological,molecular,and ecological responses.However,such adaptation often incurs significant costs,including reduced growth,yield penalties,and altered ecological interactions.This review systematically synthesizes recent advances published between 2018 and 2025,following PRISMA criteria,on plant responses to abiotic and biotic stressors,with an emphasis on the trade-offs between adaptation and productivity.It also highlights major discrepancies in the literature and discusses strategies for enhancing plant stress tolerance in agriculture.By integrating findings from genomics,transcriptomics,proteomics,and metabolomics,the review categorizes both mechanistic insights and ecological consequences.The findings underscore the need for multi-stress,systems-level,field-based research that connects molecular processes to ecological and agricultural outcomes.Accordingly,critical gaps are identified—particularly the scarcity of multi-stress and field-based studies—and future directions that integrate omics approaches,systems biology,and eco-physiological frameworks are proposed.Understanding the costs of adaptation is essential not only for breeding resilient,high-yielding crops but also for ensuring their successful incorporation into sustainable agricultural practices under changing climate conditions.展开更多
Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro ...Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro regeneration of the Kirik bread wheat cultivar via somatic embryogenesis was investigated using endospermsupported mature embryos.Callus cultures were initiated from mature embryos supported by endosperm,cultured on phytagel-based Murashige and Skoog(MS)basal mediumcontaining dicamba(12mg/L)and indole-3-acetic acid(IAA)(0.5 mg/L)under dark conditions.This research was designed to examine the impact of putrescine(Put)(0.0 and 1.0 mM)on inducing embryonic callus and the effects of thidiazuron(TDZ)(0.0,0.1,0.2,0.3,0.4,and 0.5 mg/L)on wheat regeneration.Adding 1.0mM putrescine to MS mediumsignificantly increased(p<0.01)embryogenic callus formation,resulting in a complete(100%)induction rate.Moreover,the highest number of regenerated plants per explant(5.8)was obtained through the synergistic interaction between 1.0 mM putrescine and 0.5 mg/L TDZ.To assess the genetic homogeneity of regenerated plants,10 different inter-simple sequence repeat(ISSR)primers were utilized,revealing a high level of genetic stability.The results of all the applications of a particular plant tissue culture technique showed a level of somaclonal variation within acceptable limits,indicating that the genetic diversity of the plant populations was protectedwithout compromising the desired traits.These improvements offer a promising tool forwheat biotechnology,especially for genetic transformation.展开更多
Plants undergo dynamic morphological changes in response to fluctuating light conditions.Despite significant progress in elucidating the mechanisms of light signal transduction,the precise influence of light on the de...Plants undergo dynamic morphological changes in response to fluctuating light conditions.Despite significant progress in elucidating the mechanisms of light signal transduction,the precise influence of light on the development and regulation of shoot architecture remains a central research question.Studies focusing on model plants such as Arabidopsis thaliana and rice suggest light modulates shoot architecture through intricate regulatory networks.However,the molecular mechanisms governing the diverse effects of light on horticultural crops are still poorly understood.This review primarily focuses on horticultural crops,integrating research on model plants,including Arabidopsis and rice,to provide an overview of the regulatory mechanisms of light signals in plant architectural development.It also explores the prospects for manipulating light environments in greenhouse management strategies.展开更多
Autophagy is a universal cellular process in eukaryotes that plays a critical role in plant growth and stress response.However,the role of autophagy in fruit ripening is largely unknown.Here,we demonstrated that most ...Autophagy is a universal cellular process in eukaryotes that plays a critical role in plant growth and stress response.However,the role of autophagy in fruit ripening is largely unknown.Here,we demonstrated that most autophagy-related genes(ATGs)were up-regulated during tomato(Solanum lycopersicum L.)fruit ripening.By using mutants of different autophagy pathway genes(ATG6,ATG10,ATG18a),we revealed that the deficiency of autophagy delayed the ripening of fruit.Compared with wild-type(WT),the production of ethylene was significantly reduced and the accumulation of lycopene was delayed in atg mutants during fruit ripening.We also observed the contents of glucose and fructose were both significantly decreased in atg mutants compared with WT,while the content of organic acids showed the opposite trend.Additionally,the negative regulator of ethylene production,APETALA2a(AP2a),interacted with ATG8 through a specific ATG8-interacting motif(AIM)and could be degraded through the autophagy pathway.These results demonstrate that autophagy plays a critical role in fruit ripening by regulating ethylene production and the accumulation of pigments,sugars and organic acids in tomato.展开更多
Heterotrimeric G protein serves as a central hub in plant signal transduction,playing a pivotal role in integrating endogenous developmental signals and external environmental cues.While significant advances have been...Heterotrimeric G protein serves as a central hub in plant signal transduction,playing a pivotal role in integrating endogenous developmental signals and external environmental cues.While significant advances have been made in understanding G protein signaling mechanisms in model plants such as Arabidopsis and major crops like rice and maize,the precise regulatory roles in growth,development,and adaptation in horticultural crops are still poorly understood.In this review,we systematically summarize recent advances in uncovering both conserved and species-specific regulatory mechanisms of G protein signaling across diverse plant species.We also highlight key discoveries on the crosstalk between G protein-mediated pathways and other signaling cascades,such as hormone signaling,transcriptional regulation,and stress response networks.Finally,we discuss the potential applications of G protein signaling research in future crop improvement,offering new perspectives for advancing sustainable horticultural production.展开更多
The agricultural sector is notably affected by climate change,especially soybeans,which may face diminished yields because of severe water shortages.The evaluation of germplasm at morphological and molecular levels is...The agricultural sector is notably affected by climate change,especially soybeans,which may face diminished yields because of severe water shortages.The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement.This study employed 10 simple sequence repeat(SSR)markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23.The results of the screening experiment(PEG-6000)revealed that the soybean genotypes SPS13,SPS195,PGRB83,and 39982 exhibited significant correlations in growth parameters.The results of molecular characterization indicated that five out of ten molecular markers,specifically Satt373,Satt454,Satt471,Satt478,and Satt581,exhibited distinct banding patterns along with elevated levels of genetic diversity and heterozygosity.The phylogenetic analysis findings indicated that soybean genotypes were categorized into many clusters,with at least six genotypes located in cluster 5 and the most seventeen genotypes in cluster 7.The results obtained from principal component analysis indicated that PC1 explained up to 44.7%of the variance,while PC2 accounted for 17.3%.The results of the heatmap indicated that PGBR83 exhibited the highest expression in plant height,GP39982 and SPS109 in chlorophyll content,GP39982 in proline accumulation,and SPS2,GP40025,SPS69,and GP40174 in protein content,number of pods per plant,and yield per plant,whereas GP40116 and PGRA83 demonstrated consistently low expression.The results of biochemical analysis indicated that the soybean genotypes SPS13,PGRA83,SPS176,40158,SPS162,SPS195,SPS175,SPS109,and SPS80 were identified as superior sources of protein and oil content,along with genotypes such as PGRB55,SPS177,40116,and 40111,which exhibited a significant increase under drought stress conditions.The findings of this research provide complete information derived from molecular approaches on soybean genotypes,which might assist breeders in selecting parental lines to generate drought-tolerant soybean cultivars in the future.展开更多
Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contr...Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contrasting day times:in the morning with the highest levels of leaf Se and polyphenol(TP)and the lowest dry matter,and in the evening with the opposite characteristics.Compared to the control,the morning Se supply demonstrated a higher increase of root biomass(1.27–1.37 times),Se(1.82–2.85 times),TP content(1.42–1.44 times),and antioxidant activity(AOA)(1.47–1.48 times)than the evening treatment.The latter did not affect root biomass and TP levels,but increased Se(1.38–2.57 times)and AOA(1.47–1.48 times).Contrary,compared to control,the evening Se supply improved leaf parameters more significantly than the morning treatment:AOA(1.22–1.25 vs.1.12–1.17),TP(1.29–1.33 vs.1.10–1.25),and Se(7.03–8.58 vs.5.32–7.19).Similar photosynthetic pigment increase was recorded under organic and inorganic Se supply and between morning and evening treatments.Contrasting trends in root disaccharide accumulation under Se supply were recorded between morning and evening treatments,with a significant decrease of the mentioned parameter in the former case(1.27–1.15 times)and an increase in the latter(1.11–1.31 times).Contrary to other dandelion characteristics,only disaccharide root levels demonstrated higher changes under selenocystine supply,compared to selenate.The revealed phenomenon indicates the differences in root/leaf biochemical profile response to the time of Se supplementation and may become the basis of targeted production of functional food products with improved yield and nutritional value.展开更多
基金The Enhancement Project of Young Teachers Research Innovation Ability(JKC2022006)Beijing Municipal Higher Education Institutions’Teacher Team Construction Support Plan-High-Level Teaching Innovation Team(BPHR20220211)+1 种基金Beijing Higher Education Undergraduate Teaching Reform and Innovation Project(2023003)2024 Beijing University of Agriculture Student Party Members“Vanguard Force Action”Project。
文摘This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of highly skilled talents in urban agriculture in the era of smart agriculture and their cultivation pathways and practices.It proposes measures such as building multi-level practical teaching platforms,implementing the“Enjoy Horticulture”series of high-quality activities,and establishing the“1234”applied talent training model to cultivate high-quality talents that meet the development needs of modern urban horticulture industry.Taking Beijing University of Agriculture and other universities as examples,the paper analyzes the practical cases and effects of the urban horticulture discipline’s industry-education-research collaborative talent training model,which has reference significance for further improving and perfecting the urban horticulture industry-education-research collaborative talent training plan.
文摘Lignin is a significant secondary metabolite produced through the phenylpropanoid pathway.As a vital component of the plant cell wall,lignin affects various fruit characteristics,including size,seed quantity,and firmness.In this study,we conducted comprehensive identification and phylogenetic analysis of 265 Caffeic acid O-methyltransferase(COMT)genes across ten different plant species,including Vaccinium corymbosum and four other Vaccinium species.The results reveal that VcCOMT38 is a promising structural gene for the biosynthesis of lignin in blueberry.An in vitro enzymatic assay of VcCOMT38 demonstrated that it is a special enzyme in the lignin biosynthesis pathway and prefers to use caffeic acid as a substrate over 5-hydroxyferulic acid.Transient overexpression and silencing of VcCOMT38 in Vaccinium corymbosum‘Northland’fruits demonstrated that VcCOMT38 participates in lignin biosynthesis and contributes to both an increased number of immature seeds and enhanced fruit firmness.The heterologous overexpression of VcCOMT38 in Nicotiana benthamiana revealed that this gene could increase the lignin content and the syringyl/guaiacyl(S/G)ratio,which determines the maximum monomer yield during lignin depolymerization.These results highlight VcCOMT38 as a crucial gene in lignin biosynthesis and its potential for improving lignin production in industry through genetically modified woody plants.
基金Funding in the Van der Knaap laboratory is from the National Science Foundation(IOS 1564366,IOS 1732253,and USDA 2017-67013-26199).
文摘Directed breeding of horticultural crops is essential for increasing yield,nutritional content,and consumer-valued characteristics such as shape and color of the produce.However,limited genetic diversity restricts the amount of crop improvement that can be achieved through conventional breeding approaches.Natural genetic changes in cisregulatory regions of genes play important roles in shaping phenotypic diversity by altering their expression.Utilization of CRISPR/Cas editing in crop species can accelerate crop improvement through the introduction of genetic variation in a targeted manner.The advent of CRISPR/Cas-mediated cis-regulatory region engineering(cis-engineering)provides a more refined method for modulating gene expression and creating phenotypic diversity to benefit crop improvement.Here,we focus on the current applications of CRISPR/Cas-mediated cis-engineering in horticultural crops.We describe strategies and limitations for its use in crop improvement,including de novo cis-regulatory element(CRE)discovery,precise genome editing,and transgene-free genome editing.In addition,we discuss the challenges and prospects regarding current technologies and achievements.CRISPR/Cas-mediated cis-engineering is a critical tool for generating horticultural crops that are better able to adapt to climate change and providing food for an increasing world population.
文摘Organic matter increases biological activity within the root zone because it contains beneficial microbes that stimulate vital processes.This study aimed to determine the effect of Ochrobactrum anthropi added to the soil as a single or mixed solution with different concentrations of organic matter on the vegetative growth of the zinnia plant and its content of nutrients.The study was conducted with a randomized complete block design(RCBD)with three replications.The plant was grown in soil with a high salinity level of 8 ds m^(–1).Plant characteristics were estimated 30 and 60 days after planting the plant.The treatment with the addition of bio-inoculum produced the best results;within 30 days,there was a 56.89%increase in plant length;after 60 days,there was a 52.56%increase;additionally,there was a 52.56%increase in leaf count within 30 days;after 60 days,there was a 53.50%increase;and finally,there was an increase in flower count.For plants after 60 days,it reached 3.66%.With the addition of bio-inoculum to soil at a level of 3 gm kg^(–1)of organic matter,the mixing treatment achieved the highest dry weight,29.86%.The addition of bio-inoculum resulted in the largest and most significant increase in chlorophyll content in leaves,reaching 18.76%.In the mixing addition of the biological inoculum treatment,the organic content of the plant showed an increase in nitrogen(14.38%),phosphorus(21.18%),and potassium(39.75%)at 2 and 3 gm kg^(–1)organic matter,respectively.
文摘Pepper (Capsicum annuum L.) is an important agricultural crop because of the nutritional value of the fruit and its economic importance.Various techniques have been practiced to enhance pepper's productivity and nutritional value.Therefore,this study was conducted to determine the impact of different training methods and biostimulant applications on sweet pepper plants'growth,yield,and chemical composition under greenhouse conditions.For the training method,unpruned plants were compared with one stem and two stem plants.Unpruned plants had the fruit number of 33.98,fruit weight of 2.18 kg·plant^(-1),and total marketable yield of 1 090.0 kg·hm^(-2).One stem plant gave the best average fruit weight of 86.63 g,vitamin C content of 13.66 mg·kg^(-1)FW,and TSS content of 7.21%.However,two stem plants had the highest fruit setting of 62.41%,carotenoid content of 0.14 mg·kg^(-1)FW,and fruit chlorophyll content of 3.57 mg·kg^(-1)FW.For biostimulant applications,control plants were compared with the Disper Root (DR) and Disper Vital (DV).DR application significantly increased total sugar,carotenoid,fruit chlorophyll,and TSS contents compared to the control and DV applications.While,applying DV increased fruit setting,plant fruit number,weight,and total marketable yield.In addition,integrating one stem plant with the DR application improved fiber,vitamin C,and TSS contents significantly.Two stem plants,and the DV application improved fruit setting and carotenoid content.Thus,one and two stem training methods integrated with the DR and DV biostimulant applications could be considered for developing agricultural practices to obtain commercial yield and improve the nutrition values of sweet peppers,as unpruned plants without biostimulant applications have a negative impact.
文摘The reproduction of grapevine genotypes,one of the most important species in the world,while preserving their genetic characteristics,is practically done by rooting cuttings.Adventive rooting of cutting studies for seedling production in nursery conditions often remain below the expected productivity level due to biotic and abiotic stress-related reasons.Studies to increase nursery yields are still on the agenda of grapevine researchers.In this study,the effects of silver nanoparticles(AgNPs)produced by the green synthesis method using grape seed extract and AgNO3 on rooting and vegetative growth of the standard(TS 4027)cuttings taken during the dormancy period of Vitis vinifera L.cvs Ekşi Kara and GökÜzüm were investigated under greenhouse conditions.Cuttings treated by keeping in 0.1,0.2 g·L^(−1)AgNPs,0.1,0.2 g·L^(−1)IBA aqueous solutions for 24 h were planted in black,1 L volume seedling bags filled with 1:1 peat:perlite in the greenhouse,while the control was kept in pure water for 24 h and planted.Changes in sprouting rate,plant transformation rate,shoot length,shoot diameter,number of nodes,stomatal conductance,leaf temperature,photosynthetic efficiency,leaf fresh and dry weight,SPAD,root number,root length,root fresh and root weight were examined in developing seedlings.In evaluating the effects of AgNPs and Indole-3-butyric acid(IBA)treatments on cutting rooting and vegetative development,ANOVA,post hoc analysis with the Tukey test,and Principal Component Analyses(PCAs)were used to better understand and depict the correlations between the examined variables.This analysis method was performed using ggplot2 in the R Studio program.The heatmap generated by the pheatmap package was used to visualize the correlation and variation.As a result of this study,AgNPs applications were found to be more effective than IBA treatments in the rooting of grapevine cuttings and the vegetative development of young plants.In conclusion,0.1 g·L^(−1)AgNPs can be tested as a support and/or economical alternative to IBA for the promotion of rooting of cuttings and vegetative development of young plants for subsequent clonal propagation.
文摘Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation strategies are widely recommended to enhance water use efficiency,knowledge remains limited regarding their interactions with soil amendments such as biochar and the consequent impacts on reproductive traits.This study aimed to evaluate the combined effects of deficit irrigation strategies and biochar application on pollen viability and morphology in Capsicum annuum.The experiment was conducted under full,partial,and deficit irrigation regimes with and without biochar treatment,following a randomized block design.The primary parameters examined were pollen viability(viable,semi-viable,and non-viable rates),anther width and length,and pollen width and length.Microscopic measurements and statistical analyses(p≤0.05)revealed significant effects of both irrigation regimes and biochar applications.Under deficit irrigation,viable,semi-viable,and non-viable pollen rates were 29.84%,32.95%,and 37.21%,respectively,whereas the highest viable pollen rate was observed under full irrigation.In partial irrigation,viable pollen accounted for 31.67%,semi-viable for 38.81%,and non-viable for 29.49%.In plots treated with biochar under partial irrigation,anther width(1700.89μm),anther length(3805.34μm),pollen width(26.93μm),and pollen length(37.42μm)reached the highest values,while the lowest values were recorded in deficit irrigation plots without biochar.These findings emphasize the importance of integrating biochar into irrigation management to mitigate the adverse effects of water stress on pollen development.Nevertheless,further research is needed to clarify the long-term implications of these practices for reproductive success and agricultural sustainability.
文摘Bemisia tabaci is a polyphagous herbivore that feeds on a wide range of horticultural and ornamental crops cultivated under diverse ecological zones. In Sierra Leone, B. tabaci is found to infest a wide range of vegetable crops by directly feeding on phloem sap thereby inducing physiological disorders, and also serve as a vector to gemini viruses. Invariably the destructive feeding of B. tabaci affects the productivity and aesthetic values of vegetables and other horticultural crops and hence is considered a serious economic pest. A bioassay experiment was carried out by rearing B. tabaci populations on four vegetable crops under controlled laboratory conditions to determine its life table and demographic parameters. Results showed that the intrinsic rate of growth which measures the population size and growth pattern was highest for populations reared on tomato crops with the following values: rm 0.145 female female−1 day−1, the gross reproduction rate (Ro), and finite growth rate λ were highest for population reared on tomato, correspondingly the development period from egg-adult emergence was shortest with a value of 26 d. Conversely, the computed demographical parameters rm, λ and Ro for the population reared on sweet pepper were 0.106 female female−1 day−1 respectively, with a corresponding development period egg-adult emergence as 36d. The computed biological parameters for okra and garden egg varied with intermediary values between tomato and pepper host materials. The survivorship rates were quite significant for the smaller instars (Instars 1-III) with over 80% surviving to pre-pupa and pupa stage for the populations reared for all the test materials. High mortality was noticed for the pre-pupa and pupa stages as their survival rates were significantly low compared to the high survival rates of the smaller instars. Less than 50% of pupae failed to emerge to adults except for populations reared on tomato test materials where 52% emerged to adults. The study indicated tomato as the most suitable host among the four vegetable crops. Although life table and demographic parameters are invaluable information for forecasting pest populations and help in designing pest management efforts, further investigations such as the economic threshold and economic injury levels of B. tabaci population are requisite decision tools for sound pest management decisions of B. tabaci on these vegetable crops. The information obtained from this investigation would be quite relevant for extension service and pest management practitioners where mixed vegetable farming is a common practice.
文摘Newly planted apple orchards in the USA comprising the highly biennial cultivar, “Honeycrisp”, are prone to flower within the first two years from planting and set fruit. These processes limit canopy development and subsequent yield potential. GA4 + 7 can inhibit floral formation processes of apples. The timing and dose for eliminating return bloom of young “Honeycrisp” trees, however, is unclear. A factorial experimental design to test GA4 + 7 application timing and rate produced significant reductions in return bloom for both factors and their interaction. Treatment responses demonstrated that florigenic processes in “Honeycrisp” occurred early. The most pronounced reduction in return bloom followed the 2-week after full bloom (WAFB) application timing, increasing with increasing rate. The effect on return bloom was progressively diminished over the next two weeks of applications but remained significantly lower than the control. Three successive applications timed one week apart eliminated return bloom of spurs at the highest rate. Both factors and their interaction also significantly reduced return bloom of terminal buds of leaders;in contrast to floral buds on spurs, the response of terminal buds on leaders improved with delayed application timings. Timing exerted a stronger effect than rate on return flowering of the terminal buds of leaders. Leader growth was positively affected by GA4 + 7, the year of application, when three successive applications were made. Tree height, overall growth, expressed as the increase in trunk cross-sectional area, and limb number were also significantly improved by GA4 + 7 but varied in their responses to application timing and rate, and were inconsistent. Overall, successive applications of GA4 + 7 had good efficacy for inhibiting floral initiation of meristems on spurs and terminals while improving the vegetative growth of young “Honeycrisp” trees.
基金funded by the‘National Key R&D Program Subject of China’(No.2021YFD1100301)the post subsidy project of National Key R&D Program,and the Guizhou Modern Agriculture Research System(GZMARS)-Plateau characteristic vegetable industry.
文摘Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.
文摘Phalaenopsis orchids are economically important ornamental crops;however,their commercial micropropagation is often limited by poor rooting efficiency and inconsistent growth.In this study,we investigated the effects of silver nanoparticles(Ag-NPs)on the in vitro regeneration and growth of Phalaenopsis cultivar 611B to determine the optimal concentration of Ag-NPs for improved micropropagation outcomes.Shoot tip explants(2–3 mm)—derived from protocorm-like bodies were cultured on a regeneration medium containing Hyponex(20:20:20 and 6.5:6.5:19),18 g/L sugar,2 g/L peptone,0.8 g/L activated charcoal,12.5 g/L potato extract,50 mL/L apple juice,and 10 mg/L 6-benzylaminopurine(6-BA),with varying concentrations of Ag-NPs(0,0.5,1.0,2.0,and 2.5 mg/L).After 10–12 weeks,shoot and root formation,plant height,fresh weight,leaf number,and chlorophyll contents were evaluated.At 1.0 mg/L Ag-NPs,shoot regeneration(5.4 vs.2.9 shoots per explant),root induction(2.1 vs.1.4 roots per explant),and shoot formation frequency(100%vs.55%)were significantly higher than the control(0 mg/L).Fresh weight(592.4 mg)and leaf number(9.7)also showed notable increases at this concentration.Although chlorophyll a and b levels peaked at 2.0 mg/L,the difference from 1.0 mg/L was not statistically significant.These results suggest that 1.0mg/LAg-NPs is the optimal concentration for enhancing shoot and root development and improving overall plantlet quality in Phalaenopsis.The findings highlight the potential of nanomaterials to improve the efficiency of orchid tissue culture systems.
文摘This study was conducted to determine the content,distribution and transformation of iron oxides in the soils of the Middle Euphrates regions in Iraq.The study included four sites:Tuwairij area in Karbala Governorate,College of Agriculture at the University of Kufa in Najaf Governorate,College of Agriculture at the University of Qadisiyah in Diwaniyah Governorate,and the Nile District in Babylon Governorate.The results showed that the soils of Najaf and Qadisiyah were superior in terms of their content of total free iron oxides(Fet)compared to the soils of Karbala and Babylon.The relative distribution of free iron oxides was generally close among the studied sites,with a homogeneous pattern in the distribution of these oxides within the soil horizons.As for silicate iron oxides(Fes),a homogeneous pattern was observed in the soil of Babylon with its content increasing with depth,while these patterns varied in the soils of Karbala,Najaf and Qadisiyah.Regarding the ratios of crystalline iron oxides(Fed/Fet),the study showed that the Babylon and Qadisiyah soils recorded the highest values,while these values were lower in the Najaf and Karbala soils.On the other hand,amorphous iron oxides(FeO)showed similar values in the Najaf and Qadisiyah soils.In general,these results clearly showed the effect of environmental and geochemical factors of the study areas on the distribution and transformations of iron oxides in the soil of the Middle Euphrates regions.
基金supported by the Natural Science Foundation of Shandong Province,China(ZR2022MC045)the National Natural Science Foundation of China(32001584,32201876)+2 种基金the Major Science and Technology Program of Xinjiang Uygur Autonomous Region,China(2022A02008-3)the Breeding Project from Department of Science&Technology of Shandong Province,China(2022LZGC007)the Agricultural Scientific and the Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(CXGC2023A06,CXGC2023A39 and CXGC2023A46),and the Major Scientific and Technological Achievements Cultivation Program of Shandong Academy of Agricultural Sciences,China(CXGC2025E02)。
文摘Plant height(PH),primary lateral branch length(PBL),and branch number(BN)are architectural components impacting peanut pod yield,biomass production,and adaptivity to mechanical harvesting.In this study,a recombinant inbred population consisting of 181 individual lines was used to determine genetic controls of PH,PBL,and BN across three environments.Phenotypic data collected from the population demonstrated continuous distributions and transgressive segregation patterns.Broad-sense heritability of PH,PBL,and BN was found to be 0.87,0.88,and 0.92,respectively.Unconditional individual environmental analysis revealed 35 additive QTLs with phenotypic variation explained(PVE)ranging from 4.57 to 21.68%.A two-round meta-analysis resulted in 24consensus and 19 unique QTLs.Five unique QTLs exhibited pleiotropic effects and their genetic bases(pleiotropy or tight linkage)were evaluated.A joint analysis was performed to estimate the QTL by environment interaction(QEI)effects on PH,PBL,and BN,collectively explaining phenotypic variations of 10.80,11.02,and 7.89%,respectively.We identified 3 major and stable QTL regions(uq9-3,uq10-2,and uq16-1)on chromosomes 9,10,and 16,spanning1.43-1.53 Mb genomic regions.Candidate genes involved in phytohormones biosynthesis,signaling,and cell wall development were proposed to regulate these morphological traits.These results provide valuable information for further genetic studies and the development of molecular markers applicable to peanut architecture improvement.
文摘Plants are continuously exposed to abiotic and biotic stresses that threaten their growth,reproduction,and survival.Adaptation to these stresses requires complex regulatory networks that coordinate physiological,molecular,and ecological responses.However,such adaptation often incurs significant costs,including reduced growth,yield penalties,and altered ecological interactions.This review systematically synthesizes recent advances published between 2018 and 2025,following PRISMA criteria,on plant responses to abiotic and biotic stressors,with an emphasis on the trade-offs between adaptation and productivity.It also highlights major discrepancies in the literature and discusses strategies for enhancing plant stress tolerance in agriculture.By integrating findings from genomics,transcriptomics,proteomics,and metabolomics,the review categorizes both mechanistic insights and ecological consequences.The findings underscore the need for multi-stress,systems-level,field-based research that connects molecular processes to ecological and agricultural outcomes.Accordingly,critical gaps are identified—particularly the scarcity of multi-stress and field-based studies—and future directions that integrate omics approaches,systems biology,and eco-physiological frameworks are proposed.Understanding the costs of adaptation is essential not only for breeding resilient,high-yielding crops but also for ensuring their successful incorporation into sustainable agricultural practices under changing climate conditions.
文摘Wheat is a crucial crop for global food security,and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs.A practical approach for in vitro regeneration of the Kirik bread wheat cultivar via somatic embryogenesis was investigated using endospermsupported mature embryos.Callus cultures were initiated from mature embryos supported by endosperm,cultured on phytagel-based Murashige and Skoog(MS)basal mediumcontaining dicamba(12mg/L)and indole-3-acetic acid(IAA)(0.5 mg/L)under dark conditions.This research was designed to examine the impact of putrescine(Put)(0.0 and 1.0 mM)on inducing embryonic callus and the effects of thidiazuron(TDZ)(0.0,0.1,0.2,0.3,0.4,and 0.5 mg/L)on wheat regeneration.Adding 1.0mM putrescine to MS mediumsignificantly increased(p<0.01)embryogenic callus formation,resulting in a complete(100%)induction rate.Moreover,the highest number of regenerated plants per explant(5.8)was obtained through the synergistic interaction between 1.0 mM putrescine and 0.5 mg/L TDZ.To assess the genetic homogeneity of regenerated plants,10 different inter-simple sequence repeat(ISSR)primers were utilized,revealing a high level of genetic stability.The results of all the applications of a particular plant tissue culture technique showed a level of somaclonal variation within acceptable limits,indicating that the genetic diversity of the plant populations was protectedwithout compromising the desired traits.These improvements offer a promising tool forwheat biotechnology,especially for genetic transformation.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.32330094,U21A20233,323B2057)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(Grant No.SN-ZJUSIAS-0011).
文摘Plants undergo dynamic morphological changes in response to fluctuating light conditions.Despite significant progress in elucidating the mechanisms of light signal transduction,the precise influence of light on the development and regulation of shoot architecture remains a central research question.Studies focusing on model plants such as Arabidopsis thaliana and rice suggest light modulates shoot architecture through intricate regulatory networks.However,the molecular mechanisms governing the diverse effects of light on horticultural crops are still poorly understood.This review primarily focuses on horticultural crops,integrating research on model plants,including Arabidopsis and rice,to provide an overview of the regulatory mechanisms of light signals in plant architectural development.It also explores the prospects for manipulating light environments in greenhouse management strategies.
基金supported by the National Natural Science Foundation of China(Grant Nos.32302642,32272790)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(Grant No.SN-ZJU-SIAS-0011)+1 种基金Collaborative Promotion Program of Zhejiang Provincial Agricultural Technology of China(Grant No.2023ZDXT05)the Fundamental Research Funds for the Central Universities(Grant No.226-2022-00122).
文摘Autophagy is a universal cellular process in eukaryotes that plays a critical role in plant growth and stress response.However,the role of autophagy in fruit ripening is largely unknown.Here,we demonstrated that most autophagy-related genes(ATGs)were up-regulated during tomato(Solanum lycopersicum L.)fruit ripening.By using mutants of different autophagy pathway genes(ATG6,ATG10,ATG18a),we revealed that the deficiency of autophagy delayed the ripening of fruit.Compared with wild-type(WT),the production of ethylene was significantly reduced and the accumulation of lycopene was delayed in atg mutants during fruit ripening.We also observed the contents of glucose and fructose were both significantly decreased in atg mutants compared with WT,while the content of organic acids showed the opposite trend.Additionally,the negative regulator of ethylene production,APETALA2a(AP2a),interacted with ATG8 through a specific ATG8-interacting motif(AIM)and could be degraded through the autophagy pathway.These results demonstrate that autophagy plays a critical role in fruit ripening by regulating ethylene production and the accumulation of pigments,sugars and organic acids in tomato.
基金supported by the National Natural Science Foundation of China(Grant Nos.32172650,32430092)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(Grant No.SN-ZJU-SIAS-0011)+1 种基金the Fundamental Research Funds for the Central Universities of China(Grant No.226-2024-00119)the Innovative Development of Horticulture Discipline of Zhejiang University(Grant No.B231220.0005-25).
文摘Heterotrimeric G protein serves as a central hub in plant signal transduction,playing a pivotal role in integrating endogenous developmental signals and external environmental cues.While significant advances have been made in understanding G protein signaling mechanisms in model plants such as Arabidopsis and major crops like rice and maize,the precise regulatory roles in growth,development,and adaptation in horticultural crops are still poorly understood.In this review,we systematically summarize recent advances in uncovering both conserved and species-specific regulatory mechanisms of G protein signaling across diverse plant species.We also highlight key discoveries on the crosstalk between G protein-mediated pathways and other signaling cascades,such as hormone signaling,transcriptional regulation,and stress response networks.Finally,we discuss the potential applications of G protein signaling research in future crop improvement,offering new perspectives for advancing sustainable horticultural production.
基金funding with Ref.No.PMAS-AAUR/ORIC/2018 dated 08-03-2021 from Office of Research,Innovation and Commercilization,Pir Mahr Ali Shah Air Agriculture University,Rawalpindi,Pakistan for this study.
文摘The agricultural sector is notably affected by climate change,especially soybeans,which may face diminished yields because of severe water shortages.The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement.This study employed 10 simple sequence repeat(SSR)markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23.The results of the screening experiment(PEG-6000)revealed that the soybean genotypes SPS13,SPS195,PGRB83,and 39982 exhibited significant correlations in growth parameters.The results of molecular characterization indicated that five out of ten molecular markers,specifically Satt373,Satt454,Satt471,Satt478,and Satt581,exhibited distinct banding patterns along with elevated levels of genetic diversity and heterozygosity.The phylogenetic analysis findings indicated that soybean genotypes were categorized into many clusters,with at least six genotypes located in cluster 5 and the most seventeen genotypes in cluster 7.The results obtained from principal component analysis indicated that PC1 explained up to 44.7%of the variance,while PC2 accounted for 17.3%.The results of the heatmap indicated that PGBR83 exhibited the highest expression in plant height,GP39982 and SPS109 in chlorophyll content,GP39982 in proline accumulation,and SPS2,GP40025,SPS69,and GP40174 in protein content,number of pods per plant,and yield per plant,whereas GP40116 and PGRA83 demonstrated consistently low expression.The results of biochemical analysis indicated that the soybean genotypes SPS13,PGRA83,SPS176,40158,SPS162,SPS195,SPS175,SPS109,and SPS80 were identified as superior sources of protein and oil content,along with genotypes such as PGRB55,SPS177,40116,and 40111,which exhibited a significant increase under drought stress conditions.The findings of this research provide complete information derived from molecular approaches on soybean genotypes,which might assist breeders in selecting parental lines to generate drought-tolerant soybean cultivars in the future.
文摘Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contrasting day times:in the morning with the highest levels of leaf Se and polyphenol(TP)and the lowest dry matter,and in the evening with the opposite characteristics.Compared to the control,the morning Se supply demonstrated a higher increase of root biomass(1.27–1.37 times),Se(1.82–2.85 times),TP content(1.42–1.44 times),and antioxidant activity(AOA)(1.47–1.48 times)than the evening treatment.The latter did not affect root biomass and TP levels,but increased Se(1.38–2.57 times)and AOA(1.47–1.48 times).Contrary,compared to control,the evening Se supply improved leaf parameters more significantly than the morning treatment:AOA(1.22–1.25 vs.1.12–1.17),TP(1.29–1.33 vs.1.10–1.25),and Se(7.03–8.58 vs.5.32–7.19).Similar photosynthetic pigment increase was recorded under organic and inorganic Se supply and between morning and evening treatments.Contrasting trends in root disaccharide accumulation under Se supply were recorded between morning and evening treatments,with a significant decrease of the mentioned parameter in the former case(1.27–1.15 times)and an increase in the latter(1.11–1.31 times).Contrary to other dandelion characteristics,only disaccharide root levels demonstrated higher changes under selenocystine supply,compared to selenate.The revealed phenomenon indicates the differences in root/leaf biochemical profile response to the time of Se supplementation and may become the basis of targeted production of functional food products with improved yield and nutritional value.
