Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been rep...Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been reported to be a unique component of histone lysine methylation modification complex MLL3/4,regulating gene transcription by histone 3 lysine 4 methylation(H3K4me).However,it is unknown whether PA1 is involved in the epigenetic regulation in male reproductive system.展开更多
Background:To determine the clinical and endoscopic demographic characteristics of the ingestion of acidic products in the digestive endoscopy unit of the Cocody University Hospital.Methods:This was a retrospective,de...Background:To determine the clinical and endoscopic demographic characteristics of the ingestion of acidic products in the digestive endoscopy unit of the Cocody University Hospital.Methods:This was a retrospective,descriptive and analytical study on endoscopic reports,which took place for five years,from January 1,2014,to December 31,2018.All patients were admitted to the digestive endoscopy unit with the indication of ingestion of caustic products.The parameters studied:are demographic,clinical,and endoscopic.The statistical tests used were Pearson’s CHI 2 and Fisher’s exact tests.The significance threshold was set at 5%.Results:83 patients were included for an endoscopic prevalence of 1.33%.The mean age was 20.77±16.58 years,with extremes ranging from 1 to 63 years.They were divided into 34 men and 49 women for a sex ratio of 0.69.Accidental circumstances were observed in 73.49%of cases,followed by attempted autolysis(26.51%).Bleach was the most offending caustic(75.90%),followed by hydrochloric acid(14.46%).FOGD was normal in 53.01%and revealed stage I(41.54%)digestive lesions,followed by stage II(35.39%)and stage III(21.53%).The lesions were preferentially gastric(36.11%),esogastroduodenal(27.78%),and esogastric(22.23%).Based on severity,54.55%of esophageal lesions were stage IIA,53.12%were stage I gastric lesions,and 54.55%were stage IIA duodenal lesions.One case of gastric perforation was noted(1.54%).In univariate analysis,we found a statistically significant link between the age group of 1–14 years and the accidental intake of caustics(P<0.001)on the one hand and the other hand between the age group of 26 years and over and taking for autolysis(P=0.02).Likewise,bleaching was responsible for less severe lesions(P=0.006).Conclusions:The seriousness of the ingestion of caustic products requires early,specialized and multidisciplinary management to improve the short,medium,and long-term prognosis of patients.展开更多
Objective:Intrahepatic cholangiocarcinoma(ICC)and hepatocellular carcinoma(HCC)are clinically disparate primary liver cancers with etiological and biological heterogeneity.In Thailand,both cancer types represent the p...Objective:Intrahepatic cholangiocarcinoma(ICC)and hepatocellular carcinoma(HCC)are clinically disparate primary liver cancers with etiological and biological heterogeneity.In Thailand,both cancer types represent the primary cause of cancer-related deaths and are a major public health concern.展开更多
1.The endocannabinoid system in obesity and metabolic disorders As obesity and associated metabolic disorders,such as type 2 diabetes and dyslipidemia,are becoming one of the most serious health problems worldwide,dev...1.The endocannabinoid system in obesity and metabolic disorders As obesity and associated metabolic disorders,such as type 2 diabetes and dyslipidemia,are becoming one of the most serious health problems worldwide,development of effective therapies is a high priority.In the search for treatments,the recently discovered endocannabinoid system(ECS) has begun to garner attention,and a wealth of research is now展开更多
Aim of the work: Achalasia is a rare pathology whose physiopathogenesis is mysterious. The treatment is based on endoscopic pneumatic dilation (PD) and surgery. The aim of our work is to show our experiment about endo...Aim of the work: Achalasia is a rare pathology whose physiopathogenesis is mysterious. The treatment is based on endoscopic pneumatic dilation (PD) and surgery. The aim of our work is to show our experiment about endoscopic pneumatic dilation as the principal treatment suggested for patients presenting achalasia. Material and method: It is a retrospective study relating 21 cases of achalasia indexed between 2002 and 2007. The remission was judged on Eckardt’s clinical criteria. If the symptoms persist after three episodes of PD, there’s a therapy failure and the patients were then proposed to surgery or recurrent DP. The results: The Middle Age at the time of diagnosis was of 44 ± 10, 66 years (20 to 76). The sex ratio was 1, 3 (12M?9F) (p = 0.5). The diagnosis of achalasia is based on imaging, endoscopy and manometric arguments. The PD of the cardia was proposed in first intention, except two patients who were treated 6 and 15 years before by surgery. 38 dilations were performed for 21 patients with an average of 1.8 PD per patient [1-6]. The rate of good answers after the third episode of PD was about 90.47% (19 patients). There was no complication of the PD. In plain-varied analysis, no predictive factor of good answers to PD was retained. Conclusion: PD is an effective cure with a negligible morbidity and no mortality. The surgery is reserved for the failures of pneumatic dilation.展开更多
BACKGROUND Post-transplant lymphoproliferative disorder(PTLD)is a rare but highly fatal complication occurring after allogeneic hematopoietic cell transplantation(allo-HCT)or solid organ transplantation(SOT).Unlike SO...BACKGROUND Post-transplant lymphoproliferative disorder(PTLD)is a rare but highly fatal complication occurring after allogeneic hematopoietic cell transplantation(allo-HCT)or solid organ transplantation(SOT).Unlike SOT,PTLD after allo-HCT usually originates from the donor and is rarely accompanied by a loss of donor chimerism.CASE SUMMARY We report a case of Epstein-Barr virus positive PTLD manifesting as diffuse large B-cell lymphoma(DLBCL)with significantly decreased T-cell chimerism early after allo-HCT.A 30-year-old patient with acute myeloid leukemia underwent unrelated allo-HCT after first complete remission.Nearly 3 mo after transplantation,the patient developed cervical lymph node enlargement and gastric lesions,both of which were pathologically suggestive of DLBCL.Meanwhile,the patient experienced a significant and persistent decrease in T-cell chimerism.A partial remission was achieved after chemotherapy with single agent rituximab and subsequent R-CHOP combined chemotherapy.CONCLUSION The loss of T-cell chimerism and the concomitant T-cell insufficiency may be the cause of PTLD in this patient.展开更多
Despite considerable research underscoring the importance of carbohydrate intake in relation to the risk of type 2 diabetes(T2D),a comprehensive assessment of this relationship is currently lacking.We aimed to examine...Despite considerable research underscoring the importance of carbohydrate intake in relation to the risk of type 2 diabetes(T2D),a comprehensive assessment of this relationship is currently lacking.We aimed to examine the associations of various types and food sources of dietary carbohydrate intake with the risk of T2D,to evaluate potential effect modification by other factors,including genetic susceptibility,and to explore the potential mediators for such associations.The present study included 161,872 participants of the UK Biobank who were free of prevalent cancer,cardiovascular disease,or diabetes,and had at least one validated 24-h dietary recall assessment.Multivariableadjusted age-stratified Cox proportional hazard regression models were applied to estimate hazard ratios(HRs)and 95%confidence intervals(CI)for the associations of various types and food sources of dietary carbohydrate intake with risk of T2D.During a median follow-up of 13.6 years,4,176 incident cases of T2D were identified.In the multivariable-adjusted models,a greater intake of fiber,carbohydrates from whole grains,and carbohydrates from non-starchy vegetables was significantly associated with a lower risk of T2D(highest vs.lowest quantile,HR[95%CI]=0.70[0.62–0.79],0.74[0.67–0.82],and 0.83[0.75–0.92],respectively,all P for trend<0.005).In contrast,a higher intake of starch and carbohydrate from starchy vegetables was associated with an increased risk of T2D(highest vs.lowest quantile,HR[95%CI]=1.31[1.16–1.48]and 1.19[1.09–1.31],respectively,both P for trend<0.005).Replacing one serving of refined grains or starchy vegetables with an equal amount of whole grains or non-starchy vegetables was associated with 4%to 10%lower risk of T2D(all P values<0.001).The observed associations were generally similar across population subgroups,including individuals with different genetic susceptibility to T2D.Mediation analyses of the inverse association between T2D risk and isocaloric substitution of carbohydrates from refined grains with carbohydrate from whole grains demonstrated that 39.6%,43.4%,44.0%,27.8%,and 34.9%were mediated through body mass index,waistto-hip ratio,glycosylated hemoglobin,high-density lipoprotein cholesterol,and C-reactive protein,respectively.In addition,the inverse association between the isocaloric substitution of carbohydrates from starchy vegetables with carbohydrates from non-starchy vegetables and T2D was partially mediated through high-density lipoprotein cholesterol(15.9%).These findings underscore the importance of dietary modifications of carbohydrates,particularly considering types and food sources of carbohydrate intake,in the primary prevention of T2D.展开更多
Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role...Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China.展开更多
Background:With the rising global prevalence of fatty liver disease related to metabolic dysfunction,the association of this common liver condition with chronic kidney disease(CKD)has become increasingly evident.In 20...Background:With the rising global prevalence of fatty liver disease related to metabolic dysfunction,the association of this common liver condition with chronic kidney disease(CKD)has become increasingly evident.In 2020,the more inclusive term metabolic dysfunction-associated fatty liver disease(MAFLD)was proposed to replace the term non-alcoholic fatty liver disease(NAFLD).The observed association between MAFLD and CKD and our understanding that CKD can be a consequence of underlying metabolic dysfunction support the notion that individuals with MAFLD are at higher risk of having and developing CKD compared with those without MAFLD.However,to date,there is no appropriate guidance on CKD in individuals with MAFLD.Furthermore,there has been little attention paid to the link between MAFLD and CKD in the Nephrology community.Methods and Results:Using a Delphi-based approach,a multidisciplinary panel of 50 international experts from 26 countries reached a consensus on some of the open research questions regarding the link between MAFLD and CKD.Conclusions:This Delphi-based consensus statement provided guidance on the epidemiology,mechanisms,management and treatment of MAFLD and CKD,as well as the relationship between the severity of MAFLD and risk of CKD,which establish a framework for the early prevention and management of these two common and interconnected diseases.展开更多
To the Editor:Primary liver cancer is one of the most common malignant tumors in China.In 2020,410,038 new cases of liver cancer and 391,152 deaths due to liver cancer were reported in China,which places liver cancer ...To the Editor:Primary liver cancer is one of the most common malignant tumors in China.In 2020,410,038 new cases of liver cancer and 391,152 deaths due to liver cancer were reported in China,which places liver cancer as the second most common cancer-related cause of death in China;of these cancers,hepatocellular carcinoma(HCC)accounts for 85-90%.[1]HCC poses a significant threat to the lives and health of Chinese people.展开更多
Rapid and reliable nucleic acid detection methods are essential in clinical diagnostics and biotechnology.The clustered regularly interspaced short palindromic repeats(CRISPR)system is emerging as a next-generation nu...Rapid and reliable nucleic acid detection methods are essential in clinical diagnostics and biotechnology.The clustered regularly interspaced short palindromic repeats(CRISPR)system is emerging as a next-generation nucleic acid detection technology,offering versatility,convenience and rapid detection.However,CRISPR methods are significantly limited by the protospacer adjacent motif(PAM)sequence,and achieving a one-pot reaction for detecting single nucleotide variations(SNVs)within a short time still remains challenging.Here,we developed a comprehensive method for screening PAM sequences,which significantly expands the CRISPR detection scope.Additionally,we also proposed a one-pot CRISPR method,termed"SIMPLE",capable of identifying SNVs within 30 min.We applied the SIMPLE method to the clinical diagnostics of drug-resistant bacteria and the screening of cancer hotspot mutations.The SIMPLE method successfully detected drug-resistant bacteria mediated by canonical PAM TTN sequence with a sensitivity of 10 copies per reaction and achieved 100%consistency with next-generation sequencing results.Furthermore,the SIMPLE method proved effective in detecting hotspot mutations in cancer,even at a low mutation rate of 1%in the presence of high background interference mediated by non-canonical PAM ATN sequence.Therefore,the SIMPLE method not only expands the CRISPR detection scope but also offers a one-pot reaction with high specificity for SNVs identification,making it a promising tool for next-generation molecular diagnostics.展开更多
Background:Viral pathogens belonging to the orderBunyavirales pose a continuous background threat to global health,but the fact remains that they are usually neglected and their distribution is still ambiguously known...Background:Viral pathogens belonging to the orderBunyavirales pose a continuous background threat to global health,but the fact remains that they are usually neglected and their distribution is still ambiguously known.We aim to map the geographical distribution ofBunyavirales viruses and assess the environmental suitability and transmission risk of majorBunyavirales viruses in China.Methods:We assembled data on allBunyavirales viruses detected in humans,animals and vectors from multiple sources,to update distribution maps of them across China.In addition,we predicted environmental suitability at the 10 km×10 km pixel level by applying boosted regression tree models for two importantBunyavirales viruses,including Crimean-Congo hemorrhagic fever virus(CCHFV)and Rift Valley fever virus(RVFV).Based on model-projected risks and air travel volume,the imported risk of RVFV was also estimated from its endemic areas to the cities in China.Results:Here we mapped all 89 species ofBunyavirales viruses in China from January 1951 to June 2021.Nineteen viruses were shown to infect humans,including ten species first reported as human infections.A total of 447,848 cases infected withBunyavirales viruses were reported,and hantaviruses,Dabie bandavirus and Crimean-Congo hemorrhagic fever virus(CCHFV)had the severest disease burden.Model-predicted maps showed that Xinjiang and southwestern Yunnan had the highest environmental suitability for CCHFV occurrence,mainly related toHyalomma asiaticum presence,while southern China had the highest environmental suitability for Rift Valley fever virus(RVFV)transmission all year round,mainly driven by livestock density,mean precipitation in the previous month.We further identified three cities including Guangzhou,Beijing and Shanghai,with the highest imported risk of RVFV potentially from Egypt,South Africa,Saudi Arabia and Kenya.Conclusions:A variety ofBunyavirales viruses are widely distributed in China,and the two major neglectedBunyavirales viruses including CCHFV and RVFV,both have the potential for outbreaks in local areas of China.Our study can help to promote the understanding of risk distribution and disease burden ofBunyavirales viruses in China,and the risk maps of CCHFV and RVFV occurrence are crucial to the targeted surveillance and control,especially in seasons and locations at high risk.展开更多
文摘Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been reported to be a unique component of histone lysine methylation modification complex MLL3/4,regulating gene transcription by histone 3 lysine 4 methylation(H3K4me).However,it is unknown whether PA1 is involved in the epigenetic regulation in male reproductive system.
文摘Background:To determine the clinical and endoscopic demographic characteristics of the ingestion of acidic products in the digestive endoscopy unit of the Cocody University Hospital.Methods:This was a retrospective,descriptive and analytical study on endoscopic reports,which took place for five years,from January 1,2014,to December 31,2018.All patients were admitted to the digestive endoscopy unit with the indication of ingestion of caustic products.The parameters studied:are demographic,clinical,and endoscopic.The statistical tests used were Pearson’s CHI 2 and Fisher’s exact tests.The significance threshold was set at 5%.Results:83 patients were included for an endoscopic prevalence of 1.33%.The mean age was 20.77±16.58 years,with extremes ranging from 1 to 63 years.They were divided into 34 men and 49 women for a sex ratio of 0.69.Accidental circumstances were observed in 73.49%of cases,followed by attempted autolysis(26.51%).Bleach was the most offending caustic(75.90%),followed by hydrochloric acid(14.46%).FOGD was normal in 53.01%and revealed stage I(41.54%)digestive lesions,followed by stage II(35.39%)and stage III(21.53%).The lesions were preferentially gastric(36.11%),esogastroduodenal(27.78%),and esogastric(22.23%).Based on severity,54.55%of esophageal lesions were stage IIA,53.12%were stage I gastric lesions,and 54.55%were stage IIA duodenal lesions.One case of gastric perforation was noted(1.54%).In univariate analysis,we found a statistically significant link between the age group of 1–14 years and the accidental intake of caustics(P<0.001)on the one hand and the other hand between the age group of 26 years and over and taking for autolysis(P=0.02).Likewise,bleaching was responsible for less severe lesions(P=0.006).Conclusions:The seriousness of the ingestion of caustic products requires early,specialized and multidisciplinary management to improve the short,medium,and long-term prognosis of patients.
文摘Objective:Intrahepatic cholangiocarcinoma(ICC)and hepatocellular carcinoma(HCC)are clinically disparate primary liver cancers with etiological and biological heterogeneity.In Thailand,both cancer types represent the primary cause of cancer-related deaths and are a major public health concern.
基金supported by the Academy of Finland and Shanghai overseas visiting professor scholarship program
文摘1.The endocannabinoid system in obesity and metabolic disorders As obesity and associated metabolic disorders,such as type 2 diabetes and dyslipidemia,are becoming one of the most serious health problems worldwide,development of effective therapies is a high priority.In the search for treatments,the recently discovered endocannabinoid system(ECS) has begun to garner attention,and a wealth of research is now
文摘Aim of the work: Achalasia is a rare pathology whose physiopathogenesis is mysterious. The treatment is based on endoscopic pneumatic dilation (PD) and surgery. The aim of our work is to show our experiment about endoscopic pneumatic dilation as the principal treatment suggested for patients presenting achalasia. Material and method: It is a retrospective study relating 21 cases of achalasia indexed between 2002 and 2007. The remission was judged on Eckardt’s clinical criteria. If the symptoms persist after three episodes of PD, there’s a therapy failure and the patients were then proposed to surgery or recurrent DP. The results: The Middle Age at the time of diagnosis was of 44 ± 10, 66 years (20 to 76). The sex ratio was 1, 3 (12M?9F) (p = 0.5). The diagnosis of achalasia is based on imaging, endoscopy and manometric arguments. The PD of the cardia was proposed in first intention, except two patients who were treated 6 and 15 years before by surgery. 38 dilations were performed for 21 patients with an average of 1.8 PD per patient [1-6]. The rate of good answers after the third episode of PD was about 90.47% (19 patients). There was no complication of the PD. In plain-varied analysis, no predictive factor of good answers to PD was retained. Conclusion: PD is an effective cure with a negligible morbidity and no mortality. The surgery is reserved for the failures of pneumatic dilation.
文摘BACKGROUND Post-transplant lymphoproliferative disorder(PTLD)is a rare but highly fatal complication occurring after allogeneic hematopoietic cell transplantation(allo-HCT)or solid organ transplantation(SOT).Unlike SOT,PTLD after allo-HCT usually originates from the donor and is rarely accompanied by a loss of donor chimerism.CASE SUMMARY We report a case of Epstein-Barr virus positive PTLD manifesting as diffuse large B-cell lymphoma(DLBCL)with significantly decreased T-cell chimerism early after allo-HCT.A 30-year-old patient with acute myeloid leukemia underwent unrelated allo-HCT after first complete remission.Nearly 3 mo after transplantation,the patient developed cervical lymph node enlargement and gastric lesions,both of which were pathologically suggestive of DLBCL.Meanwhile,the patient experienced a significant and persistent decrease in T-cell chimerism.A partial remission was achieved after chemotherapy with single agent rituximab and subsequent R-CHOP combined chemotherapy.CONCLUSION The loss of T-cell chimerism and the concomitant T-cell insufficiency may be the cause of PTLD in this patient.
基金supported by the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0508200,2023ZD0508201)the National Natural Science Foundation of China(82100949,82470931)+2 种基金the Outstanding Young Investigator Award of Hunan Province(2022JJ10094)Central South University Research Programme of Advanced Interdisciplinary Studies(2023QYJC008)to J.Hthe Intramural Research Program of the U.S.National Cancer Institute(NCI),National Institutes of Health(NIH)to D.A。
文摘Despite considerable research underscoring the importance of carbohydrate intake in relation to the risk of type 2 diabetes(T2D),a comprehensive assessment of this relationship is currently lacking.We aimed to examine the associations of various types and food sources of dietary carbohydrate intake with the risk of T2D,to evaluate potential effect modification by other factors,including genetic susceptibility,and to explore the potential mediators for such associations.The present study included 161,872 participants of the UK Biobank who were free of prevalent cancer,cardiovascular disease,or diabetes,and had at least one validated 24-h dietary recall assessment.Multivariableadjusted age-stratified Cox proportional hazard regression models were applied to estimate hazard ratios(HRs)and 95%confidence intervals(CI)for the associations of various types and food sources of dietary carbohydrate intake with risk of T2D.During a median follow-up of 13.6 years,4,176 incident cases of T2D were identified.In the multivariable-adjusted models,a greater intake of fiber,carbohydrates from whole grains,and carbohydrates from non-starchy vegetables was significantly associated with a lower risk of T2D(highest vs.lowest quantile,HR[95%CI]=0.70[0.62–0.79],0.74[0.67–0.82],and 0.83[0.75–0.92],respectively,all P for trend<0.005).In contrast,a higher intake of starch and carbohydrate from starchy vegetables was associated with an increased risk of T2D(highest vs.lowest quantile,HR[95%CI]=1.31[1.16–1.48]and 1.19[1.09–1.31],respectively,both P for trend<0.005).Replacing one serving of refined grains or starchy vegetables with an equal amount of whole grains or non-starchy vegetables was associated with 4%to 10%lower risk of T2D(all P values<0.001).The observed associations were generally similar across population subgroups,including individuals with different genetic susceptibility to T2D.Mediation analyses of the inverse association between T2D risk and isocaloric substitution of carbohydrates from refined grains with carbohydrate from whole grains demonstrated that 39.6%,43.4%,44.0%,27.8%,and 34.9%were mediated through body mass index,waistto-hip ratio,glycosylated hemoglobin,high-density lipoprotein cholesterol,and C-reactive protein,respectively.In addition,the inverse association between the isocaloric substitution of carbohydrates from starchy vegetables with carbohydrates from non-starchy vegetables and T2D was partially mediated through high-density lipoprotein cholesterol(15.9%).These findings underscore the importance of dietary modifications of carbohydrates,particularly considering types and food sources of carbohydrate intake,in the primary prevention of T2D.
文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China.
文摘Background:With the rising global prevalence of fatty liver disease related to metabolic dysfunction,the association of this common liver condition with chronic kidney disease(CKD)has become increasingly evident.In 2020,the more inclusive term metabolic dysfunction-associated fatty liver disease(MAFLD)was proposed to replace the term non-alcoholic fatty liver disease(NAFLD).The observed association between MAFLD and CKD and our understanding that CKD can be a consequence of underlying metabolic dysfunction support the notion that individuals with MAFLD are at higher risk of having and developing CKD compared with those without MAFLD.However,to date,there is no appropriate guidance on CKD in individuals with MAFLD.Furthermore,there has been little attention paid to the link between MAFLD and CKD in the Nephrology community.Methods and Results:Using a Delphi-based approach,a multidisciplinary panel of 50 international experts from 26 countries reached a consensus on some of the open research questions regarding the link between MAFLD and CKD.Conclusions:This Delphi-based consensus statement provided guidance on the epidemiology,mechanisms,management and treatment of MAFLD and CKD,as well as the relationship between the severity of MAFLD and risk of CKD,which establish a framework for the early prevention and management of these two common and interconnected diseases.
基金supported by grants from the State Key Project of the National Natural Science Foundation of China(No.81730097)the Clinical Science and Technology Innovation Project of Shanghai Shenkang Hospital Development Center(Joint Project of Emerging Frontier Technology)(No.SHDC12018116)the Clinical Research Plan of Shanghai Hospital Development Center(No.SHDC2020CR1004A).
文摘To the Editor:Primary liver cancer is one of the most common malignant tumors in China.In 2020,410,038 new cases of liver cancer and 391,152 deaths due to liver cancer were reported in China,which places liver cancer as the second most common cancer-related cause of death in China;of these cancers,hepatocellular carcinoma(HCC)accounts for 85-90%.[1]HCC poses a significant threat to the lives and health of Chinese people.
基金supported by the National Natural Science Foundation of China(32371521,82300220)Special Project for Experimental Animal Research(23141900300)+3 种基金Shanghai Rising Star Program(23QA1404300)Special Project for Medical Innovation Research(22Y11909200)Greater Bay Area Institute of Precision Medicine(Guangzhou)Human Phenome Data Center of Fudan University and Shanghai Municipal Science and Technology Major Project.(2023SHZDZX02).
文摘Rapid and reliable nucleic acid detection methods are essential in clinical diagnostics and biotechnology.The clustered regularly interspaced short palindromic repeats(CRISPR)system is emerging as a next-generation nucleic acid detection technology,offering versatility,convenience and rapid detection.However,CRISPR methods are significantly limited by the protospacer adjacent motif(PAM)sequence,and achieving a one-pot reaction for detecting single nucleotide variations(SNVs)within a short time still remains challenging.Here,we developed a comprehensive method for screening PAM sequences,which significantly expands the CRISPR detection scope.Additionally,we also proposed a one-pot CRISPR method,termed"SIMPLE",capable of identifying SNVs within 30 min.We applied the SIMPLE method to the clinical diagnostics of drug-resistant bacteria and the screening of cancer hotspot mutations.The SIMPLE method successfully detected drug-resistant bacteria mediated by canonical PAM TTN sequence with a sensitivity of 10 copies per reaction and achieved 100%consistency with next-generation sequencing results.Furthermore,the SIMPLE method proved effective in detecting hotspot mutations in cancer,even at a low mutation rate of 1%in the presence of high background interference mediated by non-canonical PAM ATN sequence.Therefore,the SIMPLE method not only expands the CRISPR detection scope but also offers a one-pot reaction with high specificity for SNVs identification,making it a promising tool for next-generation molecular diagnostics.
基金National Key Research and Development Program of China(2021YFC2302004)National Science Foundation for Distinguished Young Scholars of China(81825019)。
文摘Background:Viral pathogens belonging to the orderBunyavirales pose a continuous background threat to global health,but the fact remains that they are usually neglected and their distribution is still ambiguously known.We aim to map the geographical distribution ofBunyavirales viruses and assess the environmental suitability and transmission risk of majorBunyavirales viruses in China.Methods:We assembled data on allBunyavirales viruses detected in humans,animals and vectors from multiple sources,to update distribution maps of them across China.In addition,we predicted environmental suitability at the 10 km×10 km pixel level by applying boosted regression tree models for two importantBunyavirales viruses,including Crimean-Congo hemorrhagic fever virus(CCHFV)and Rift Valley fever virus(RVFV).Based on model-projected risks and air travel volume,the imported risk of RVFV was also estimated from its endemic areas to the cities in China.Results:Here we mapped all 89 species ofBunyavirales viruses in China from January 1951 to June 2021.Nineteen viruses were shown to infect humans,including ten species first reported as human infections.A total of 447,848 cases infected withBunyavirales viruses were reported,and hantaviruses,Dabie bandavirus and Crimean-Congo hemorrhagic fever virus(CCHFV)had the severest disease burden.Model-predicted maps showed that Xinjiang and southwestern Yunnan had the highest environmental suitability for CCHFV occurrence,mainly related toHyalomma asiaticum presence,while southern China had the highest environmental suitability for Rift Valley fever virus(RVFV)transmission all year round,mainly driven by livestock density,mean precipitation in the previous month.We further identified three cities including Guangzhou,Beijing and Shanghai,with the highest imported risk of RVFV potentially from Egypt,South Africa,Saudi Arabia and Kenya.Conclusions:A variety ofBunyavirales viruses are widely distributed in China,and the two major neglectedBunyavirales viruses including CCHFV and RVFV,both have the potential for outbreaks in local areas of China.Our study can help to promote the understanding of risk distribution and disease burden ofBunyavirales viruses in China,and the risk maps of CCHFV and RVFV occurrence are crucial to the targeted surveillance and control,especially in seasons and locations at high risk.
