To investigate the correlation and difference between Paspalum notatum’s leaf and root under drought adversity,this study tested the physiological responses of wild Paspalum notatum’s leaf and root under 0(CK),1,3,5...To investigate the correlation and difference between Paspalum notatum’s leaf and root under drought adversity,this study tested the physiological responses of wild Paspalum notatum’s leaf and root under 0(CK),1,3,5,7,14,21,and 28 d drought stress treatment.These physiological responses include osmotic regulating substance,antioxidant enzyme activity,malonaldehyde content,cell membrane permeability,and chlorophyll content.According to the results,the soluble protein content,proline concentration,malonaldehyde content,cell membrane permeability and CAT activity in Paspalum notatum’s leaf and root tended to increase with the prolongation of drought stress;the soluble sugar content and SOD activity first increased and then decreased;POD dropped gradually;the contents of chlorophyll a,b and total chlorophyll first declined,then mounted,and lastly dropped again.During the early stage of drought stress,SOD and CAT acted as the main antioxidant enzymes in Paspalum notatum’s leaf and root.However,with the aggravation of drought stress the predominant antioxidant enzymes became SOD in the root system whereas CAT and POD in the leaf.It implicates drought has a strong influence on root’s CAT and leaf’s SOD and POD.Leaf is the major organ regulating the osmosis and photosynthesis of Paspalum notatum.The findings can provide a useful theoretical basis for improving Paspalum notatum’s drought tolerance.展开更多
Schisandrin B(Sch B)is a monomer with anti-cancer and anti-inflammatory effects,which are isolated from the plant Schisandra chinensis(Turcz)Baillon.We investigated the anti-gastric cancer(GC)effects of Sch B and its ...Schisandrin B(Sch B)is a monomer with anti-cancer and anti-inflammatory effects,which are isolated from the plant Schisandra chinensis(Turcz)Baillon.We investigated the anti-gastric cancer(GC)effects of Sch B and its underlying molecular mechanisms.The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines.Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B.Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins.The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen species(ROS)levels and related signaling pathways in GC cells.The results showed that Sch B could regulate the phosphorylation level of mitogen-activated protein kinase(MAPK)by upregulating ROS accumulation in gastric cancer cells,and then reduce the expression of nuclear factor kappa B(NF-κB)and phosphorylated transcription 3(p-STAT3).In addition,Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,and arrested cells in the G0/G1 phase.Moreover,it also inhibited cell migration,which was reversed with Nacetylcysteine pretreatment.In summary,Sch B has killing effects on GC cells by upregulating the production of intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway,leading to the migration arrest and apoptosis of GC cells.展开更多
Marine collagen peptides(MCPs)are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes.MCPs have a range of structures and biological activities and are wid...Marine collagen peptides(MCPs)are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes.MCPs have a range of structures and biological activities and are widely present in marine species.MCPs also have a small molecular weight,are easily modified,and absorbed by the body.These properties have attracted great interest from researchers studying antioxidant,anti-tumor,and anti-aging activities.MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects.Thus,MCPs have the potential use as anti-cancer adjuvant drugs.Free radicals produced by oxidation are closely related to human aging,cancer,arteriosclerosis,and other diseases,but their relationship with cancer is not well known.In this review,we focus on the antioxidant properties of MCPs in the treatment of cancer,highlighting their antioxidant molecular structure and potential for clinical practice.展开更多
基金Supported by National Key Research&Development Project(2016YFC0502603,2017YFD0502101-3)National Natural Science Foundation of China(31602005)+2 种基金Science and Technology Support Project of Guizhou Province(Qiankehe Support[2016]2516,Qiankehe Cheng Zhuan [2015]5324-2)Scientific and Technol-ogical Innovation Talents Team Construction Project(Qiankehe Platform Talents[2016]5617)Guizhou Province Outstanding Youth and Scientifictechnological Talents Cultivation Project(Qiankehe Ren[2015]02)~~
文摘To investigate the correlation and difference between Paspalum notatum’s leaf and root under drought adversity,this study tested the physiological responses of wild Paspalum notatum’s leaf and root under 0(CK),1,3,5,7,14,21,and 28 d drought stress treatment.These physiological responses include osmotic regulating substance,antioxidant enzyme activity,malonaldehyde content,cell membrane permeability,and chlorophyll content.According to the results,the soluble protein content,proline concentration,malonaldehyde content,cell membrane permeability and CAT activity in Paspalum notatum’s leaf and root tended to increase with the prolongation of drought stress;the soluble sugar content and SOD activity first increased and then decreased;POD dropped gradually;the contents of chlorophyll a,b and total chlorophyll first declined,then mounted,and lastly dropped again.During the early stage of drought stress,SOD and CAT acted as the main antioxidant enzymes in Paspalum notatum’s leaf and root.However,with the aggravation of drought stress the predominant antioxidant enzymes became SOD in the root system whereas CAT and POD in the leaf.It implicates drought has a strong influence on root’s CAT and leaf’s SOD and POD.Leaf is the major organ regulating the osmosis and photosynthesis of Paspalum notatum.The findings can provide a useful theoretical basis for improving Paspalum notatum’s drought tolerance.
基金This work was supported by the National Natural Science Foundation of China[Grant No.82060118]the Research Program of Science and Technology at Universities of Inner Mongolia Autonomous Region[Grant No.NJZY20203]+3 种基金the Program for Young Talents of Chifeng University[Grant No.CFXYYT2202]the Central Government Supports Local College Reform and Development Fund Talent Training Projects[Grant No.2020GSP16]the Heilongjiang Touyan Innovation Team Program[Grant No.2019HTY078]the Project for Heilongjiang Bayi Agricultural University[Grant No.XDB202012].
文摘Schisandrin B(Sch B)is a monomer with anti-cancer and anti-inflammatory effects,which are isolated from the plant Schisandra chinensis(Turcz)Baillon.We investigated the anti-gastric cancer(GC)effects of Sch B and its underlying molecular mechanisms.The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines.Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B.Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins.The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen species(ROS)levels and related signaling pathways in GC cells.The results showed that Sch B could regulate the phosphorylation level of mitogen-activated protein kinase(MAPK)by upregulating ROS accumulation in gastric cancer cells,and then reduce the expression of nuclear factor kappa B(NF-κB)and phosphorylated transcription 3(p-STAT3).In addition,Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,and arrested cells in the G0/G1 phase.Moreover,it also inhibited cell migration,which was reversed with Nacetylcysteine pretreatment.In summary,Sch B has killing effects on GC cells by upregulating the production of intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway,leading to the migration arrest and apoptosis of GC cells.
基金supported by the Central Government Supports Local College Reform and Development Fund Talent Training Projects[Grant Number 2020GSP16]the Heilongjiang Touyan Innovation Team Program[Grant Number 2019HTY078].
文摘Marine collagen peptides(MCPs)are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes.MCPs have a range of structures and biological activities and are widely present in marine species.MCPs also have a small molecular weight,are easily modified,and absorbed by the body.These properties have attracted great interest from researchers studying antioxidant,anti-tumor,and anti-aging activities.MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects.Thus,MCPs have the potential use as anti-cancer adjuvant drugs.Free radicals produced by oxidation are closely related to human aging,cancer,arteriosclerosis,and other diseases,but their relationship with cancer is not well known.In this review,we focus on the antioxidant properties of MCPs in the treatment of cancer,highlighting their antioxidant molecular structure and potential for clinical practice.
