Summary： Adult-bom neurons undergo a transient period of plasticity during their integration into the neural circuit. This transient plasticity may involve NMDA receptors containing NR2B, the major sub unit expressed...Summary： Adult-bom neurons undergo a transient period of plasticity during their integration into the neural circuit. This transient plasticity may involve NMDA receptors containing NR2B, the major sub unit expressed at early developmental stages. The main objective of the present study was to investigate the effects of NR2B gene knockdown on the functional integration of the adult-born granule cells gen- erated from the subgranule zone （SGZ） in the hippocampus. The small interfering RNA （siRNA） was used to knock down the NR2B gene in the adult-born hippocampal neurons. In the functional integration test, the mice were exposed to a novel environment （open field arena）, and the expression of c-fos was immunohistochemically detected in the hippocampus. After exposure to the novel environment, siRNA-NR2B mice were significantly different from control mice in either the number of squares or the number of rears they crossed, showing decreased horizontal and vertical activity （P〈0.05）. Moreover, the c-fos expression was increased in both control and siRNA-NR2B mice after open field test. But, it was significantly lower in siRNA-NR2B neurons than in control neurons. It was concluded that the neu- ral activity of newborn neurons is regulated by their own NR2B-containing NMDA glutamate receptors during a short, critical period after neuronal birth.展开更多
in elderly patients with ischemic cardiomyopathy(ICM).MethodsClinical data of patients with coronary heart discase who were discharged from The First Affiliated Hospital,Chongqing Medical University between 2005 and 2...in elderly patients with ischemic cardiomyopathy(ICM).MethodsClinical data of patients with coronary heart discase who were discharged from The First Affiliated Hospital,Chongqing Medical University between 2005 and 2007 were analyzed retrospectively.Echocardiography results,plasma hemoglobin and creatinine concentration were abstracted from the medical records.The study included 235Chinese Han patients with age 60 years and older with angiography confirmed coronary heart disease,silent myocardial ischemia or angina pectoris,of whom 154 had ICM defined as left ventricular end-diastolic diameter(LVDd),male≥56 mm,female≥51 mm(63.51±7.70 mm)measured by M-mode echocardiography.The differences in plasma hemoglobin concentration were analyzed retrospec-tively between patients with and without 1CM,and between patients with an eGFR<60 ml·min'·1.73mand those with an eGFR≥60 ml.min.1.73m².Results There were no significant differences in plasma hemoglobin concentration and eGFR between ICM and non-ICM group(118.49±20.52g·L'vs.115.80±23.32 g*L'and 75.13±24.21 ml·min'.1.73m²vs.79.09±28.41 ml.min'.1.73m²,respectively,both P>0.05).However,in both ICM and non-ICMgroups,plasma hemoglobin concentration was lower in those with an eGFR<60 ml·min'.1.73m2compared with compared with those with an eGFR≥60 ml·min'.1.73m2 group(112.29±18.61 g·L'vs.119.92±20.74L',P<0.05);plasma hemoglobin concentration was related positively to eGFR.Conclusions There were no significant changes in plasma hemoglobin concentration and eGFR;however,plasma hemoglobin concentration was related to ObjectivesTo study the relationship between plasma hemoglobin concentration and estimated glomerular filtration rate(eGFR)eGFR significantly positively in elderly patients with ICM due to coronary heart disease.展开更多
Background:Carotid intima-media thickness(IMT)and diameter,stiffness,and wave reflections,are independent and important clinical biomarkers and risk predictors for cardiovascular diseases.The purpose of the present st...Background:Carotid intima-media thickness(IMT)and diameter,stiffness,and wave reflections,are independent and important clinical biomarkers and risk predictors for cardiovascular diseases.The purpose of the present study was to establish nationwide reference values of carotid properties for healthy Chinese adults and to explore potential clinical determinants.Methods:A total of 3053 healthy Han Chinese adults(1922 women)aged 18-79 years were enrolled at 28 collaborating tertiary centers throughout China between April 2021 and July 2022.The real-time tracking of common carotid artery walls was achieved by the radio frequency(RF)ultrasound system.The IMT,diameter,compliance coefficient,βstiffness,local pulse wave velocity(PWV),local systolic blood pressure,augmented pressure(AP),and augmentation index(AIx)were then automatically measured and reported.Data were stratified by age groups and sex.The relationships between age and carotid property parameters were analyzed by Jonckheere-Terpstra test and simple linear regressions.The major clinical determinants of carotid properties were identified by Pearson’s correlation,multiple linear regression,and analyses of covariance.Results:All the parameters of carotid properties demonstrated significantly age-related trajectories.Women showed thinner IMT,smaller carotid diameter,larger AP,and AIx than men.Theβstiffness and PWV were significantly higher in men than women before forties,but the differences reversed after that.The increase rate of carotid IMT(5.5µm/year in women and 5.8µm/year in men)and diameter(0.03 mm/year in both men and women)were similar between men and women.For the stiffness and wave reflections,women showed significantly larger age-related variations than men as demonstrated by steeper regression slopes(all P for age by sex interaction<0.05).The blood pressures,body mass index(BMI),and triglyceride levels were identified as major clinical determinants of carotid properties with adjustment of age and sex.Conclusions:The age-and sex-specific reference values of carotid properties measured by RF ultrasound for healthy Chinese adults were established.The blood pressures,BMI,and triglyceride levels should be considered for clinical application of corresponding reference values.展开更多
基金supported by the National Natural Science Foundation of China(No.81000537)
文摘Summary： Adult-bom neurons undergo a transient period of plasticity during their integration into the neural circuit. This transient plasticity may involve NMDA receptors containing NR2B, the major sub unit expressed at early developmental stages. The main objective of the present study was to investigate the effects of NR2B gene knockdown on the functional integration of the adult-born granule cells gen- erated from the subgranule zone （SGZ） in the hippocampus. The small interfering RNA （siRNA） was used to knock down the NR2B gene in the adult-born hippocampal neurons. In the functional integration test, the mice were exposed to a novel environment （open field arena）, and the expression of c-fos was immunohistochemically detected in the hippocampus. After exposure to the novel environment, siRNA-NR2B mice were significantly different from control mice in either the number of squares or the number of rears they crossed, showing decreased horizontal and vertical activity （P〈0.05）. Moreover, the c-fos expression was increased in both control and siRNA-NR2B mice after open field test. But, it was significantly lower in siRNA-NR2B neurons than in control neurons. It was concluded that the neu- ral activity of newborn neurons is regulated by their own NR2B-containing NMDA glutamate receptors during a short, critical period after neuronal birth.
基金the Medical Science&Technology Research fund of Health Bureau of Chongqing City,China[No.2004(53)04-2-154]the Nature Science Research fund of Chongqing Science&Technology Com-mission of Chongqing City,China(No.CSTC,2007BB5276).
文摘in elderly patients with ischemic cardiomyopathy(ICM).MethodsClinical data of patients with coronary heart discase who were discharged from The First Affiliated Hospital,Chongqing Medical University between 2005 and 2007 were analyzed retrospectively.Echocardiography results,plasma hemoglobin and creatinine concentration were abstracted from the medical records.The study included 235Chinese Han patients with age 60 years and older with angiography confirmed coronary heart disease,silent myocardial ischemia or angina pectoris,of whom 154 had ICM defined as left ventricular end-diastolic diameter(LVDd),male≥56 mm,female≥51 mm(63.51±7.70 mm)measured by M-mode echocardiography.The differences in plasma hemoglobin concentration were analyzed retrospec-tively between patients with and without 1CM,and between patients with an eGFR<60 ml·min'·1.73mand those with an eGFR≥60 ml.min.1.73m².Results There were no significant differences in plasma hemoglobin concentration and eGFR between ICM and non-ICM group(118.49±20.52g·L'vs.115.80±23.32 g*L'and 75.13±24.21 ml·min'.1.73m²vs.79.09±28.41 ml.min'.1.73m²,respectively,both P>0.05).However,in both ICM and non-ICMgroups,plasma hemoglobin concentration was lower in those with an eGFR<60 ml·min'.1.73m2compared with compared with those with an eGFR≥60 ml·min'.1.73m2 group(112.29±18.61 g·L'vs.119.92±20.74L',P<0.05);plasma hemoglobin concentration was related positively to eGFR.Conclusions There were no significant changes in plasma hemoglobin concentration and eGFR;however,plasma hemoglobin concentration was related to ObjectivesTo study the relationship between plasma hemoglobin concentration and estimated glomerular filtration rate(eGFR)eGFR significantly positively in elderly patients with ICM due to coronary heart disease.
基金supported by the grants from the Key Research and Development Program of Shaanxi Province(No.2023-ZDLSF-22)National Key R&D Program of China(No.2023YFA1801200)+4 种基金National Natural Science Foundation of China(Nos.81901751 and 82272010)Key Clinical Trial Program of Tangdu Hospital(No.2021LCYJ006)Program for Innovative Research Team of Shaanxi Province(No.2020TD-038)Top Talent Program of Tangdu Hospital and Innovative Talent Support Program of Shaanxi Province(No.2022KJXX-106)the Special Fund for Aerospace Medical Research.
文摘Background:Carotid intima-media thickness(IMT)and diameter,stiffness,and wave reflections,are independent and important clinical biomarkers and risk predictors for cardiovascular diseases.The purpose of the present study was to establish nationwide reference values of carotid properties for healthy Chinese adults and to explore potential clinical determinants.Methods:A total of 3053 healthy Han Chinese adults(1922 women)aged 18-79 years were enrolled at 28 collaborating tertiary centers throughout China between April 2021 and July 2022.The real-time tracking of common carotid artery walls was achieved by the radio frequency(RF)ultrasound system.The IMT,diameter,compliance coefficient,βstiffness,local pulse wave velocity(PWV),local systolic blood pressure,augmented pressure(AP),and augmentation index(AIx)were then automatically measured and reported.Data were stratified by age groups and sex.The relationships between age and carotid property parameters were analyzed by Jonckheere-Terpstra test and simple linear regressions.The major clinical determinants of carotid properties were identified by Pearson’s correlation,multiple linear regression,and analyses of covariance.Results:All the parameters of carotid properties demonstrated significantly age-related trajectories.Women showed thinner IMT,smaller carotid diameter,larger AP,and AIx than men.Theβstiffness and PWV were significantly higher in men than women before forties,but the differences reversed after that.The increase rate of carotid IMT(5.5µm/year in women and 5.8µm/year in men)and diameter(0.03 mm/year in both men and women)were similar between men and women.For the stiffness and wave reflections,women showed significantly larger age-related variations than men as demonstrated by steeper regression slopes(all P for age by sex interaction<0.05).The blood pressures,body mass index(BMI),and triglyceride levels were identified as major clinical determinants of carotid properties with adjustment of age and sex.Conclusions:The age-and sex-specific reference values of carotid properties measured by RF ultrasound for healthy Chinese adults were established.The blood pressures,BMI,and triglyceride levels should be considered for clinical application of corresponding reference values.
