The yeast, Saccharomyces cerevisiae, has an ENOX1 activity with a period length of 24 min similar to that of other eukaryotes. In contrast to other eukaryotes, however, Saccharomyces cerevisiae has a second ENOX1-like...The yeast, Saccharomyces cerevisiae, has an ENOX1 activity with a period length of 24 min similar to that of other eukaryotes. In contrast to other eukaryotes, however, Saccharomyces cerevisiae has a second ENOX1-like activity with a period length of 25 min. The latter is distinguishable from the traditional ENOX1 on the basis of the longer period length along with resistance to an ENOX1 inhibitor, simalikalactone D, and failure to be phased by melatonin. In addition, two periods are apparent in measurements of oxygen consumption indicating that the consumption of oxygen to water occurs independently by homodimers of both of the two forms of ENOX. Based on the measurements of glyceraldehyde-3- phosphate dehydrogenase, S. cerevisiae exhibits circadian activity maxima at 24 and 25 h together with a 40 h period possibly representing the 40 min metabolic rhythm of yeast not observed in our measurement of oxygen consumption and normally observed only with continuous cultures. The findings are indicative of at least three independent time-keeping systems being operative in a single cell.展开更多
Objective: To investigate the antioxidant and anti-infective potential of Phrynium capitatum and Dryptes indica extract. Methods: The antioxidant potentials were determined by DPPH radical scavenging, reducing power, ...Objective: To investigate the antioxidant and anti-infective potential of Phrynium capitatum and Dryptes indica extract. Methods: The antioxidant potentials were determined by DPPH radical scavenging, reducing power, hydroxyl radical scavenging and total antioxidant assays. We further examined anti-quorum sensing activity and inhibition of synthesis of pathogenic factor of Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Bioactive compounds were determined using gas chromatography–mass spectrometry analysis. In silico analysis was conducted to determine the binding affinity of bioactive compounds of plant extracts for the quorum sensing regulatory receptor LasR. Results: DPPH assay showed that the ethanolic extract of Phrynium capitatum and Dryptes indica at 500 μg/mL showed (86.96 ± 4.07)% and (74.83 ± 3.47)% inhibition, respectively. Hydroxyl radical scavenging assay showed (73.17 ± 3.03)% and (62.63 ± 4.59)% activity, respectively. The ethanolic extract of Phrynium capitatum and Dryptes indica showed high level of attenuation of quorum sensing regulated pyocyanin production. Confocal laser scanning microscopic analysis revealed that the extracts had the potential to effectively inhibit biofilm formation of Pseudomonas aeruginosa. Molecular docking analysis showed a better binding affinity of bioactive compounds from the extracts for the structure of LasR protein of Pseudomonas aeruginosa. Conclusions: The ethanolic extracts of Phrynium capitatum and Dryptes indica possess antioxidant activity and the potential to inhibit the quorum sensing system and its regulatory virulence traits in Pseudomonas aeruginosa PAO1.展开更多
Aim: The aim of the present study was to examine associations among maternal stress level, general health, mother to infant bonding, maternal depression level, and mode of delivery. Methods: Mothers who delivered a si...Aim: The aim of the present study was to examine associations among maternal stress level, general health, mother to infant bonding, maternal depression level, and mode of delivery. Methods: Mothers who delivered a single baby at term were recruited with a total 435 mothers participating in the study. Outcome measures: Data were collected 6 months after delivery using General Health Questionnaire (GHQ-28), Mother-to-Infant Bonding Scale (MIBS), Edinburgh Postnatal Depression Scale (EPDS) as well as socio-demographic and medical information at 6 months. Additionally, salivary cortisol and chromogranin levels were determined. Results: The proportion of breast-feeding mothers in the vaginal delivery group (51.4%) was significantly higher than that of the cesarean section group (24%). GHQ-28 scores were significantly related to EPDS scores and MIBS scores (P Conclusion: Maternal general health is associated with maternal psychological health. These results suggest that the support of maternal health is important to maintain maternal psychological status and bonding to infants. Because EPDS scores were significantly worse in the planned cesarean section group (4.2 ± 3.3), careful management is needed of mothers who deliver by planned cesarean section.展开更多
Background: Diet plays a vital role in managing diabetes. Foods with a low glycemic index provide lower postprandial glucose spikes and induce satiety. The objective of this study was to assess the Glycemic index (GI)...Background: Diet plays a vital role in managing diabetes. Foods with a low glycemic index provide lower postprandial glucose spikes and induce satiety. The objective of this study was to assess the Glycemic index (GI) without milk and Glycemic response (GR) with milk of two different flavours of a plant-based supplement which is high in protein and fibre, along with a subjective assessment of satiety. Methods: Fifteen overweight/obese subjects aged 18 - 45 years were recruited. After overnight fasting, blood samples were drawn at 5 mins before food consumption (-5), 0, 15, 30, 45, 60, 90 and 120 minutes. Participants underwent 3 days of reference food testing and 1 day of test food in random order with 2 days of wash-out period. The GI was assessed using a validated protocol recognized by FAO/WHO, as well as the guidelines by the International Dietary Carbohydrate Task Force for GI Methodology. The satiety index was measured using the Visual Analog Scale (VAS). The dietary intake of the subjects was measured by 24-hour dietary recall. The Incremental Area Under the Curve (IAUC) was calculated using the trapezoid rule. Results: Both the flavours of the supplement had low GI & GR. The GI and GR of Flavour 1 were 27.3 ± 4.8 & 16.4 ± 2.6 (Mean ± SEM) respectively. For Flavour 2 the GI and GR were 36.7 ± 4.4 & 25.7 ± 2.3 (Mean ± SEM). For Flavour 1, 60% and for flavour 2 66.7% of subjects reported feeling hungry only after 3 hours, showing good satiety. Conclusion: The plant-based high fibre high protein supplement in both flavours showed a low glycemic index and hence may be useful to include in the diets to reduce the postprandial glycemic response and could improve satiety.展开更多
A yeast (Saccharomyces cerevisiae) deletion library was screened based on NADH fluorescence using a 384 well plate assay and robotics to identify a yeast isolate lacking the 24 min periodic cell surface oxidase. The o...A yeast (Saccharomyces cerevisiae) deletion library was screened based on NADH fluorescence using a 384 well plate assay and robotics to identify a yeast isolate lacking the 24 min periodic cell surface oxidase. The oxidase was shown previously to be a candidate ultradian oscillator of the yeast’s biological clock. The cDNA was cloned from a yeast overexpression library and the encoded protein was expressed in bacteria and characterized. Glyceraldehyde-3-phosphate dehydrogenase activity was used as the cellular circadian indicator. The identified gene was YML117W which encodes a ca 126 kDa putative RNA-binding protein. The candidate ENOX1 activity from yeast had functional characteristics similar to those of other constitutive ENOX1 proteins of eukaryotes exhibiting oscillating activities with a temperature independent period length of 24 min phased by melatonin and low frequency electromagnetic fields and susceptible to inhibition by the ENOX1 inhibitor, simalikalactone D. The YML117W deletion mutant cells lacked the ENOX1 clock output present in wild type yeast. The findings identify YML117W as the ENOX1 of Saccharomyces cerevisiae and support its proposed function as an ultradian oscillator of the yeast biological clock.展开更多
With increasing incidence of diabetes, use of diabetes specific nutrition supplements (DSNS) is common for better management of the disease. To study effect of 12-week DSNS supplementation on glycemic markers, anthrop...With increasing incidence of diabetes, use of diabetes specific nutrition supplements (DSNS) is common for better management of the disease. To study effect of 12-week DSNS supplementation on glycemic markers, anthropometry, lipid profile, SCFAs, and gut microbiome in individuals with diabetes. Markers studied were glycemic [Fasting Blood Glucose (FBG), Post Prandial Glucose (PPG), HbA1c, Incremental Area under curve (iAUC), Mean Amplitude of Glycemic Excursions (MAGE), Time in/above Range (TIR/TAR)], anthropometry [weight, Body Mass Index (BMI), waist circumference (WC)], lipid profile, diet and gut health [plasma short chain fatty acids (SCFAs)]. N = 210 adults were randomized to receive either DSNS with standard care (DSNS + SC;n = 105) or standard care alone (SC alone;n = 105). After 12 weeks, significant differences between DSNS + SC versus SC alone was observed in FBG [−3 ± 6 vs 14 ± 6 mg/dl;p = 0.03], PPG [−35 ± 9 vs −3 ± 9 mg/dl;p = 0.01], weight [−0.6 ± 0.1 vs 0.2 ± 0.1 kg;p = 0.0001], BMI [−0.3 ± 0.1 vs 0.1 ± 0.1 kg/m2;p = 0.0001] and WC [−0.3 ± 0.2 vs 0.2 ± 0.2 cm;p = 0.01]. HbA1C and low-density lipoprotein (LDL) were significantly reduced in DSNS + SC [−0.2 ± 0.9;p = 0.04 and −5 mg/dl;p = 0.03] respectively with no change in control. Continuous Glucose Monitoring (CGM) reported significant differences between DSNS + SC versus SC alone for mean glucose [−12 ± 65 vs 28 ± 93 mg/dl;p < 0.01], TAR 180 [−9 ± 42 vs 7 ± 45 mg/dl;p = 0.04], TAR 250 [−3 ± 27 vs 9 ± 38 mg/dl;p = 0.05], iAUC [−192 (1.1) vs −48 (1.1) mg/dl;p = 0.03]. MAGE was significantly reduced for both DSNS + SC (−19 ± 67;p < 0.001) and SC alone (−8 ± 70;p = 0.04), with reduction being more pronounced for DSNS + SC. DSNS + SC reported a decrease in carbohydrate energy % [−9.4 (−11.3, −7.6) %;p < 0.0001] and amount [−47.4 (−67.1, −27.7) g;p < 0.0001], increased dietary fiber [9.5 (7.2, 11.8) g;p < 0.0001] and protein energy % [0.9 (0.5, 1.3) %;p < 0.0001] versus SC alone. DSNS + SC reported significant increases versus SC alone in total (0.3 ng/ml;p = 0.03) and individual plasma SCFAs. The consumption of DSNS significantly improves the glycemic, anthropometric, dietary, and gut health markers in diabetes.展开更多
Carbohydrate, in the form of glucose, is the preferred fuel for working muscles particularly during high intensity activity. Some carbohydrate will be used up irrespective of the type of exercise performed. A study wa...Carbohydrate, in the form of glucose, is the preferred fuel for working muscles particularly during high intensity activity. Some carbohydrate will be used up irrespective of the type of exercise performed. A study was conducted to assess the knowledge of adolescent female football players regarding carbohydrate and its importance. It was found that 70% subjects were aware of the term carbohydrate. The awareness of the subjects regarding the term carbohydrate and its association with the function of carbohydrate is highly significant （P 〈 0.001）. The awareness level of the subjects on carbohydrate sources, type of carbohydrate to be consumed before, after and during competition was not significant. A need for developing nutrition education tools and imparting nutrition education programs becomes pertinent to enable the players to choose appropriate diet to enhance their performance.展开更多
Background and Objectives:Evidence suggests that bioactive components present in plant foods have beneficial effects.Fenugreek is commonly used in Indian culinary practices and can help to keep blood sugar levels unde...Background and Objectives:Evidence suggests that bioactive components present in plant foods have beneficial effects.Fenugreek is commonly used in Indian culinary practices and can help to keep blood sugar levels under control.The objective of this study was to assess the efficacy of defatted fenugreek seed flakes(Fenuflakes^(TM))on the 24-hour glycaemic response(Incremental Area Under the Curve,iAUC),variability(Mean Amplitude Glycaemic Excursion assessed by Continuous Glucose Monitoring system)in participants with type 2 diabetes.展开更多
Sugar loaded confectioneries have a rapidly growing market globally.Consumption of such products may lead to multiple health risks.Products like gummies can be substituted with healthy sugar alternatives such as Galac...Sugar loaded confectioneries have a rapidly growing market globally.Consumption of such products may lead to multiple health risks.Products like gummies can be substituted with healthy sugar alternatives such as Galactooligosaccharide(GOS),which can be consumed by all age groups.The aim of the study was to develop standard gummies and sugar substituted gummies,conduct sensory analysis,shelf life studies and characterize it for its physico chemical properties.The standard gummies were made using agar,sugar,citric acid,water,and FSSAI(Food Safety Standards Authority of India)certified natural colours and flavours,while GOS supplemented gummies were made by replacing sugar in varied amounts,upto 100%.A trained panel(n=8)evaluated the gummies using a composite score card in triplicates for a variety of sensory attributes.GOS recovery analysis,physicochemical variables such as colour,moisture,pH,and texture were assessed.Shelf life Studies of 100 percent GOS supplemented gummies were carried out at accelerated temperatures(37℃)over a period of 6 months.The results revealed that gummies with varying levels of GOS were acceptable to the panelists,with no significant differences in the keeping quality.However,F test revealed a significant improvement(p<0.05)in the texture of the gummies with a slight reduction in colour and flavour at the end of 6 months.The moisture content and pH values were 24.8%and 3.37 respectively.HPLC analysis revealed a recovery of 95%GOS in the prepared gummies.Hence,sugar can be substituted with 100%GOS to fulfil the increasing demand for healthy confectioneries without any change in organoleptic qualities and shelf life for 6 months.展开更多
This paper aims to identify the antecedents of buying behavior for secondhand clothing among millennials,as well as to determine their underlying causal relationships.Upon a comprehensive literature search,a total of ...This paper aims to identify the antecedents of buying behavior for secondhand clothing among millennials,as well as to determine their underlying causal relationships.Upon a comprehensive literature search,a total of 18 antecedents were found,and these are categorized into three motives,namely,economic,hedonic and recreational,and critical.As a case study in the Philippines,a focus group discussion among experts who are active millennial secondhand clothing users and buyers were tasked to identify the antecedents they have experienced and further confirm those extracted from the literature.To establish the causal relationships of these antecedents,categorize them into net causes or net effects,and address the vagueness associated with the decision-making process,a fuzzy DEMATEL method is used.Results reveal that avoidance of conventional channels proves to be the antecedent providing the highest impact among all other antecedents.Uniqueness,high quality,and fashion trend found to be the antecedents with the highest impacts received,making them the major net effects.Findings from this work hope to provide a framework among practitioners that would lead to a better understanding of millennials’buying behavior for secondhand clothing.展开更多
Malnutrition is almost universally present in patients undergoing liver transplantation.In this report,a male adult patient was followed from his pre-liver transplant phase until chronic post-transplant phase(3 months...Malnutrition is almost universally present in patients undergoing liver transplantation.In this report,a male adult patient was followed from his pre-liver transplant phase until chronic post-transplant phase(3 months after the transplant).Improvement in nutrition status,quality of life,and performance status was seen from the pre-transplant to chronic post-transplant phase.Day to day nutrition monitoring and gradual increase in calorie and protein intake was seen in the acute post-transplant phase,but during pre-and chronic post-transplant phase,lack of nutrition support was observed in the patient.展开更多
Emerging research has suggested that inflammatory stress may play a role in the development of obesity.Both the leptin and insulin receptor are sensitive to intracellular inflammatory signaling that can be stimulated ...Emerging research has suggested that inflammatory stress may play a role in the development of obesity.Both the leptin and insulin receptor are sensitive to intracellular inflammatory signaling that can be stimulated through toll-like receptor 4 activation by saturated fat. Pharmacological intervention within this cascade often protects animals from becoming obese, thus highlighting inflammatory pathways as a possible site of study in the prevention of pathologic weight gain. It has been well established in animal models that females display a marked reduction in the susceptibility to weight gain on high-fat diets compared to males. In addition, it has been widely accepted that females are partially protected from inflammatory-related diseases. At the molecular level, this reduction in disease susceptibility has been suggested to be due to the anti-inflammatory properties of 17 β-estradiol. Through direct free radical scavenging, transcriptional regulation, and protein interactions, chronic exposure to estradiol can reduce systemic inflammatory stress. As the knowledge base continues to grow on the etiology of obesity, further research is needed on the precise molecular pathways that can be inhibited by estradiol. Understanding of such pathways may provide a basis for the future use of estrogen and its related compounds （daidzein, genistein, resveratrol） to prevent weight gain in peri- and post-menopausal females.展开更多
The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon pow...The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon powder(Cinnamomum verum),Timur(Zanthoxylum alatum),Tulsi(Holy basil)(Ocimum tenuiflorum),Gandraini(Achillea millefolium),Lemon grass(Cymbopogon citratus),Blend(Clove+Timur+Gandraini),and one only corn silk without any other herbs and to know antioxidant properties of developed tea.Result of sensory evaluation experiment showed that all combinations were liked moderately.Lemon grass flavour followed by cinnamon and clove blend obtained highest score.Total phenolic content,total flavonoid content,DPPH free radical scavenging activity and total antioxidant activity for all flavour are analyzed and reported.Results on DPPH free radical scavenging activity indicated that corn silk tea had 81.215±0.75μg/mL whereas blend with Timur had significant highest value and Thyme blend had lowest value 64.20±0.40 among all eight different blends with corn silk.The values for total antioxidant capacity blends with Timur(Zanthoxylum alatu)in corn silk showed significantly(P<0.05)highest value and Clove,Timur,Gandraini blends got lowest value.展开更多
Malnutrition is universally prevalent among pre-liver transplantation patients.Malnutrition among cirrhotic patients had been associated to increased morbidity and mortality rates.Also,severely malnourished patients b...Malnutrition is universally prevalent among pre-liver transplantation patients.Malnutrition among cirrhotic patients had been associated to increased morbidity and mortality rates.Also,severely malnourished patients before the transplant surgery have a higher rate of complications and a decreased overall survival rate after liver transplantation.In light of the high incidence of malnutrition and associated complications,it is essential to initiate treatment as early as it is assessed.This review addresses the aetiologies of malnutrition and appropriate treatment strategies to correct it in pre-liver transplant phase.Treatment should focus on maintaining nutrient intake and correcting various nutritional deficiencies.The dietician plays an integral role as part of the transplant team by providing appropriate nutrition therapy for solving various nutrition problems.展开更多
文摘The yeast, Saccharomyces cerevisiae, has an ENOX1 activity with a period length of 24 min similar to that of other eukaryotes. In contrast to other eukaryotes, however, Saccharomyces cerevisiae has a second ENOX1-like activity with a period length of 25 min. The latter is distinguishable from the traditional ENOX1 on the basis of the longer period length along with resistance to an ENOX1 inhibitor, simalikalactone D, and failure to be phased by melatonin. In addition, two periods are apparent in measurements of oxygen consumption indicating that the consumption of oxygen to water occurs independently by homodimers of both of the two forms of ENOX. Based on the measurements of glyceraldehyde-3- phosphate dehydrogenase, S. cerevisiae exhibits circadian activity maxima at 24 and 25 h together with a 40 h period possibly representing the 40 min metabolic rhythm of yeast not observed in our measurement of oxygen consumption and normally observed only with continuous cultures. The findings are indicative of at least three independent time-keeping systems being operative in a single cell.
文摘Objective: To investigate the antioxidant and anti-infective potential of Phrynium capitatum and Dryptes indica extract. Methods: The antioxidant potentials were determined by DPPH radical scavenging, reducing power, hydroxyl radical scavenging and total antioxidant assays. We further examined anti-quorum sensing activity and inhibition of synthesis of pathogenic factor of Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Bioactive compounds were determined using gas chromatography–mass spectrometry analysis. In silico analysis was conducted to determine the binding affinity of bioactive compounds of plant extracts for the quorum sensing regulatory receptor LasR. Results: DPPH assay showed that the ethanolic extract of Phrynium capitatum and Dryptes indica at 500 μg/mL showed (86.96 ± 4.07)% and (74.83 ± 3.47)% inhibition, respectively. Hydroxyl radical scavenging assay showed (73.17 ± 3.03)% and (62.63 ± 4.59)% activity, respectively. The ethanolic extract of Phrynium capitatum and Dryptes indica showed high level of attenuation of quorum sensing regulated pyocyanin production. Confocal laser scanning microscopic analysis revealed that the extracts had the potential to effectively inhibit biofilm formation of Pseudomonas aeruginosa. Molecular docking analysis showed a better binding affinity of bioactive compounds from the extracts for the structure of LasR protein of Pseudomonas aeruginosa. Conclusions: The ethanolic extracts of Phrynium capitatum and Dryptes indica possess antioxidant activity and the potential to inhibit the quorum sensing system and its regulatory virulence traits in Pseudomonas aeruginosa PAO1.
文摘Aim: The aim of the present study was to examine associations among maternal stress level, general health, mother to infant bonding, maternal depression level, and mode of delivery. Methods: Mothers who delivered a single baby at term were recruited with a total 435 mothers participating in the study. Outcome measures: Data were collected 6 months after delivery using General Health Questionnaire (GHQ-28), Mother-to-Infant Bonding Scale (MIBS), Edinburgh Postnatal Depression Scale (EPDS) as well as socio-demographic and medical information at 6 months. Additionally, salivary cortisol and chromogranin levels were determined. Results: The proportion of breast-feeding mothers in the vaginal delivery group (51.4%) was significantly higher than that of the cesarean section group (24%). GHQ-28 scores were significantly related to EPDS scores and MIBS scores (P Conclusion: Maternal general health is associated with maternal psychological health. These results suggest that the support of maternal health is important to maintain maternal psychological status and bonding to infants. Because EPDS scores were significantly worse in the planned cesarean section group (4.2 ± 3.3), careful management is needed of mothers who deliver by planned cesarean section.
文摘Background: Diet plays a vital role in managing diabetes. Foods with a low glycemic index provide lower postprandial glucose spikes and induce satiety. The objective of this study was to assess the Glycemic index (GI) without milk and Glycemic response (GR) with milk of two different flavours of a plant-based supplement which is high in protein and fibre, along with a subjective assessment of satiety. Methods: Fifteen overweight/obese subjects aged 18 - 45 years were recruited. After overnight fasting, blood samples were drawn at 5 mins before food consumption (-5), 0, 15, 30, 45, 60, 90 and 120 minutes. Participants underwent 3 days of reference food testing and 1 day of test food in random order with 2 days of wash-out period. The GI was assessed using a validated protocol recognized by FAO/WHO, as well as the guidelines by the International Dietary Carbohydrate Task Force for GI Methodology. The satiety index was measured using the Visual Analog Scale (VAS). The dietary intake of the subjects was measured by 24-hour dietary recall. The Incremental Area Under the Curve (IAUC) was calculated using the trapezoid rule. Results: Both the flavours of the supplement had low GI & GR. The GI and GR of Flavour 1 were 27.3 ± 4.8 & 16.4 ± 2.6 (Mean ± SEM) respectively. For Flavour 2 the GI and GR were 36.7 ± 4.4 & 25.7 ± 2.3 (Mean ± SEM). For Flavour 1, 60% and for flavour 2 66.7% of subjects reported feeling hungry only after 3 hours, showing good satiety. Conclusion: The plant-based high fibre high protein supplement in both flavours showed a low glycemic index and hence may be useful to include in the diets to reduce the postprandial glycemic response and could improve satiety.
文摘A yeast (Saccharomyces cerevisiae) deletion library was screened based on NADH fluorescence using a 384 well plate assay and robotics to identify a yeast isolate lacking the 24 min periodic cell surface oxidase. The oxidase was shown previously to be a candidate ultradian oscillator of the yeast’s biological clock. The cDNA was cloned from a yeast overexpression library and the encoded protein was expressed in bacteria and characterized. Glyceraldehyde-3-phosphate dehydrogenase activity was used as the cellular circadian indicator. The identified gene was YML117W which encodes a ca 126 kDa putative RNA-binding protein. The candidate ENOX1 activity from yeast had functional characteristics similar to those of other constitutive ENOX1 proteins of eukaryotes exhibiting oscillating activities with a temperature independent period length of 24 min phased by melatonin and low frequency electromagnetic fields and susceptible to inhibition by the ENOX1 inhibitor, simalikalactone D. The YML117W deletion mutant cells lacked the ENOX1 clock output present in wild type yeast. The findings identify YML117W as the ENOX1 of Saccharomyces cerevisiae and support its proposed function as an ultradian oscillator of the yeast biological clock.
文摘With increasing incidence of diabetes, use of diabetes specific nutrition supplements (DSNS) is common for better management of the disease. To study effect of 12-week DSNS supplementation on glycemic markers, anthropometry, lipid profile, SCFAs, and gut microbiome in individuals with diabetes. Markers studied were glycemic [Fasting Blood Glucose (FBG), Post Prandial Glucose (PPG), HbA1c, Incremental Area under curve (iAUC), Mean Amplitude of Glycemic Excursions (MAGE), Time in/above Range (TIR/TAR)], anthropometry [weight, Body Mass Index (BMI), waist circumference (WC)], lipid profile, diet and gut health [plasma short chain fatty acids (SCFAs)]. N = 210 adults were randomized to receive either DSNS with standard care (DSNS + SC;n = 105) or standard care alone (SC alone;n = 105). After 12 weeks, significant differences between DSNS + SC versus SC alone was observed in FBG [−3 ± 6 vs 14 ± 6 mg/dl;p = 0.03], PPG [−35 ± 9 vs −3 ± 9 mg/dl;p = 0.01], weight [−0.6 ± 0.1 vs 0.2 ± 0.1 kg;p = 0.0001], BMI [−0.3 ± 0.1 vs 0.1 ± 0.1 kg/m2;p = 0.0001] and WC [−0.3 ± 0.2 vs 0.2 ± 0.2 cm;p = 0.01]. HbA1C and low-density lipoprotein (LDL) were significantly reduced in DSNS + SC [−0.2 ± 0.9;p = 0.04 and −5 mg/dl;p = 0.03] respectively with no change in control. Continuous Glucose Monitoring (CGM) reported significant differences between DSNS + SC versus SC alone for mean glucose [−12 ± 65 vs 28 ± 93 mg/dl;p < 0.01], TAR 180 [−9 ± 42 vs 7 ± 45 mg/dl;p = 0.04], TAR 250 [−3 ± 27 vs 9 ± 38 mg/dl;p = 0.05], iAUC [−192 (1.1) vs −48 (1.1) mg/dl;p = 0.03]. MAGE was significantly reduced for both DSNS + SC (−19 ± 67;p < 0.001) and SC alone (−8 ± 70;p = 0.04), with reduction being more pronounced for DSNS + SC. DSNS + SC reported a decrease in carbohydrate energy % [−9.4 (−11.3, −7.6) %;p < 0.0001] and amount [−47.4 (−67.1, −27.7) g;p < 0.0001], increased dietary fiber [9.5 (7.2, 11.8) g;p < 0.0001] and protein energy % [0.9 (0.5, 1.3) %;p < 0.0001] versus SC alone. DSNS + SC reported significant increases versus SC alone in total (0.3 ng/ml;p = 0.03) and individual plasma SCFAs. The consumption of DSNS significantly improves the glycemic, anthropometric, dietary, and gut health markers in diabetes.
文摘Carbohydrate, in the form of glucose, is the preferred fuel for working muscles particularly during high intensity activity. Some carbohydrate will be used up irrespective of the type of exercise performed. A study was conducted to assess the knowledge of adolescent female football players regarding carbohydrate and its importance. It was found that 70% subjects were aware of the term carbohydrate. The awareness of the subjects regarding the term carbohydrate and its association with the function of carbohydrate is highly significant （P 〈 0.001）. The awareness level of the subjects on carbohydrate sources, type of carbohydrate to be consumed before, after and during competition was not significant. A need for developing nutrition education tools and imparting nutrition education programs becomes pertinent to enable the players to choose appropriate diet to enhance their performance.
基金funded by Indus Biotech,Ltd.(India)(Study number:IBS448)。
文摘Background and Objectives:Evidence suggests that bioactive components present in plant foods have beneficial effects.Fenugreek is commonly used in Indian culinary practices and can help to keep blood sugar levels under control.The objective of this study was to assess the efficacy of defatted fenugreek seed flakes(Fenuflakes^(TM))on the 24-hour glycaemic response(Incremental Area Under the Curve,iAUC),variability(Mean Amplitude Glycaemic Excursion assessed by Continuous Glucose Monitoring system)in participants with type 2 diabetes.
文摘Sugar loaded confectioneries have a rapidly growing market globally.Consumption of such products may lead to multiple health risks.Products like gummies can be substituted with healthy sugar alternatives such as Galactooligosaccharide(GOS),which can be consumed by all age groups.The aim of the study was to develop standard gummies and sugar substituted gummies,conduct sensory analysis,shelf life studies and characterize it for its physico chemical properties.The standard gummies were made using agar,sugar,citric acid,water,and FSSAI(Food Safety Standards Authority of India)certified natural colours and flavours,while GOS supplemented gummies were made by replacing sugar in varied amounts,upto 100%.A trained panel(n=8)evaluated the gummies using a composite score card in triplicates for a variety of sensory attributes.GOS recovery analysis,physicochemical variables such as colour,moisture,pH,and texture were assessed.Shelf life Studies of 100 percent GOS supplemented gummies were carried out at accelerated temperatures(37℃)over a period of 6 months.The results revealed that gummies with varying levels of GOS were acceptable to the panelists,with no significant differences in the keeping quality.However,F test revealed a significant improvement(p<0.05)in the texture of the gummies with a slight reduction in colour and flavour at the end of 6 months.The moisture content and pH values were 24.8%and 3.37 respectively.HPLC analysis revealed a recovery of 95%GOS in the prepared gummies.Hence,sugar can be substituted with 100%GOS to fulfil the increasing demand for healthy confectioneries without any change in organoleptic qualities and shelf life for 6 months.
文摘This paper aims to identify the antecedents of buying behavior for secondhand clothing among millennials,as well as to determine their underlying causal relationships.Upon a comprehensive literature search,a total of 18 antecedents were found,and these are categorized into three motives,namely,economic,hedonic and recreational,and critical.As a case study in the Philippines,a focus group discussion among experts who are active millennial secondhand clothing users and buyers were tasked to identify the antecedents they have experienced and further confirm those extracted from the literature.To establish the causal relationships of these antecedents,categorize them into net causes or net effects,and address the vagueness associated with the decision-making process,a fuzzy DEMATEL method is used.Results reveal that avoidance of conventional channels proves to be the antecedent providing the highest impact among all other antecedents.Uniqueness,high quality,and fashion trend found to be the antecedents with the highest impacts received,making them the major net effects.Findings from this work hope to provide a framework among practitioners that would lead to a better understanding of millennials’buying behavior for secondhand clothing.
文摘Malnutrition is almost universally present in patients undergoing liver transplantation.In this report,a male adult patient was followed from his pre-liver transplant phase until chronic post-transplant phase(3 months after the transplant).Improvement in nutrition status,quality of life,and performance status was seen from the pre-transplant to chronic post-transplant phase.Day to day nutrition monitoring and gradual increase in calorie and protein intake was seen in the acute post-transplant phase,but during pre-and chronic post-transplant phase,lack of nutrition support was observed in the patient.
文摘Emerging research has suggested that inflammatory stress may play a role in the development of obesity.Both the leptin and insulin receptor are sensitive to intracellular inflammatory signaling that can be stimulated through toll-like receptor 4 activation by saturated fat. Pharmacological intervention within this cascade often protects animals from becoming obese, thus highlighting inflammatory pathways as a possible site of study in the prevention of pathologic weight gain. It has been well established in animal models that females display a marked reduction in the susceptibility to weight gain on high-fat diets compared to males. In addition, it has been widely accepted that females are partially protected from inflammatory-related diseases. At the molecular level, this reduction in disease susceptibility has been suggested to be due to the anti-inflammatory properties of 17 β-estradiol. Through direct free radical scavenging, transcriptional regulation, and protein interactions, chronic exposure to estradiol can reduce systemic inflammatory stress. As the knowledge base continues to grow on the etiology of obesity, further research is needed on the precise molecular pathways that can be inhibited by estradiol. Understanding of such pathways may provide a basis for the future use of estrogen and its related compounds （daidzein, genistein, resveratrol） to prevent weight gain in peri- and post-menopausal females.
文摘The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon powder(Cinnamomum verum),Timur(Zanthoxylum alatum),Tulsi(Holy basil)(Ocimum tenuiflorum),Gandraini(Achillea millefolium),Lemon grass(Cymbopogon citratus),Blend(Clove+Timur+Gandraini),and one only corn silk without any other herbs and to know antioxidant properties of developed tea.Result of sensory evaluation experiment showed that all combinations were liked moderately.Lemon grass flavour followed by cinnamon and clove blend obtained highest score.Total phenolic content,total flavonoid content,DPPH free radical scavenging activity and total antioxidant activity for all flavour are analyzed and reported.Results on DPPH free radical scavenging activity indicated that corn silk tea had 81.215±0.75μg/mL whereas blend with Timur had significant highest value and Thyme blend had lowest value 64.20±0.40 among all eight different blends with corn silk.The values for total antioxidant capacity blends with Timur(Zanthoxylum alatu)in corn silk showed significantly(P<0.05)highest value and Clove,Timur,Gandraini blends got lowest value.
文摘Malnutrition is universally prevalent among pre-liver transplantation patients.Malnutrition among cirrhotic patients had been associated to increased morbidity and mortality rates.Also,severely malnourished patients before the transplant surgery have a higher rate of complications and a decreased overall survival rate after liver transplantation.In light of the high incidence of malnutrition and associated complications,it is essential to initiate treatment as early as it is assessed.This review addresses the aetiologies of malnutrition and appropriate treatment strategies to correct it in pre-liver transplant phase.Treatment should focus on maintaining nutrient intake and correcting various nutritional deficiencies.The dietician plays an integral role as part of the transplant team by providing appropriate nutrition therapy for solving various nutrition problems.
