The contamination of Atlantic salmon with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has impeded the development of the cold-chain food industry and posed possible risks to the population.Electron beam...The contamination of Atlantic salmon with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has impeded the development of the cold-chain food industry and posed possible risks to the population.Electron beam(E-beam)irradiation under 2,4,7,and 10 kGy can effectively inactivate SARS-CoV-2 in cold-chain seafood.However,there are few statistics about the quality changes of salmon exposed to these irradiation dosages.This work demonstrated that E-beam irradiation at dosages capable of killing SARS-CoV-2 induced lipid oxidation,decreased vitamin A content,and increased some amino acids and ash content.In addition,irradiation altered the textural features of salmon,such as its hardness,resilience,cohesiveness,and chewiness.The irradiation considerably affected the L*,a*,and b*values of salmon,with the L*value increasing and a*,b*values decreasing.There was no significant difference in the sensory evaluation of control and irradiated salmon.It was shown that irradiation with 2−7 kGy E-beam did not significantly degrade quality.The inactivation of SARS-CoV-2 in salmon is advised at a dose of 2 kGy.展开更多
Synthetic color is added to foods to replace natural color lost during processing. It is causes health problems mainly in children that considered Avery venerable group. This study was carried out investigate the effe...Synthetic color is added to foods to replace natural color lost during processing. It is causes health problems mainly in children that considered Avery venerable group. This study was carried out investigate the effect of using color foods (Color fruit juice for 6 - 12 hr. Tomato ketchup potato chips (TKPC) color sweet and color chocolate at low (15%) and high (30%)) concentration on serum biochemical, WBC (white blood cell)and histopathology of liver and kidney of rats for 13 week. The results indicated that no significant change in body weight gain, serum glucose, HDL and LDL cholesterol. TKPC (30%) showed that significant increase in total cholesterol (TC), triacylglycerol (TG). Low and high concentration of color chocolate and color sweet was exhibited significant increase of TG. The level of ALT and AST was significant increase of rat’s administration color fruit juice (for 12 hr.) and TKPC at 30%. High concentration of color foods and long time administration of color fruit juice showed significant increase in serum creatinine and albumin compared to control group. Both low and high consumed of color foods exhibited significant decrease in liver GSH. High concentration of color foods lead to increase number of WBC as the result to the response of the immune system to the inflammation. Color foods were reveled change in histological structure of liver and kidney. In conclusion, the use synthetic color in various foods has adverse effect on some of biochemical analysis;and the liver and kidney histopathological structure.展开更多
CHD （coronary heart disease） is the single most important cause of death and, more importantly, the single biggest cause of premature death in modem, industrialized countries. On the basis of summarized results in r...CHD （coronary heart disease） is the single most important cause of death and, more importantly, the single biggest cause of premature death in modem, industrialized countries. On the basis of summarized results in respect to assessing file nutritional status and dietary pattern of coronary heart diseased patients, it was concluded that nutritional status of control group was better as compared to diseased group. The dietary pattern of diseased group was similar to control group.展开更多
Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might b...Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might be responsible for the property of invasiveness and/or therapies-resistance.CSCs are a self-renewing,awfully tumorigenic subpopulation of cancer cells,which are notorious for strong chemoresistance and are frequently responsible the aggravated invasion,metastasis,and/or recurrence.Developing targeting therapies against CSCs,therefore,may be deliberated a more encouraging mission for the greater cancer therapy.Innovation for a more potent anti-CSC treatment has been required as soon as possible.Interestingly,vitamin D could modulate the inflammatory condition of the tumor microenvironment(TME)by successfully affecting CSCs,which has an imperative role in determining the malignant phenotype of CSCs.In addition,vitamin D may also contribute to the regulation of the malignant behaviors of CSCs.Consistently,vitamin D could have potential applications for the significant inhibition of several tumor growths within various cancer therapies.The biological significance of vitamin D for CSCs regulation may be involved in the function of APRO family proteins.Therefore,vitamin D could be one of the innovative therapeutic modalities for the development of novel CSCs related tumor therapies.展开更多
The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several differen...The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several different bacteria,can actively contribute to the regulation of host metabolism,immunity,and inflammation.Roles of ncRNAs and gut microbiota could significantly interact with each other to regulate the growth of various types of cancer.In particular,a causal relationship among ncRNAs,gut microbiota,and immune cells has been shown for their potential importance in the development of breast cancer.Alteration of ncRNA expression and/or gut microbiota profiles could also influence several intracellular signaling pathways with the function of anti-proliferative(APRO)family proteins associated with the malignancy.Targeting ncRNAs and/or APRO family proteins for the treatment of various cancers has been revealed with novel immune therapies.Here,the most recent studies to underline the key role of ncRNAs,APRO family proteins,and gut microbiota in breast cancer progression have been discussed.For more effective breast cancer therapy,it would be imperative to figure out the collective mechanism of ncRNAs,APRO family proteins,and gut microbiota.展开更多
Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and li...Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and lifestyle-related factors. This study aimed to assess the prevalence of these conditions in the urban commune of Dubréka, in Guinea, and to assess the role of demographic and cultural factors in their incidence and management. A cross-sectional study was conducted with 287 participants (174 women and 113 men) in Dubréka. Data were summer collected on the characteristics of sociodemographics, BMI, blood pressure and fasting blood glucose. The prevalence of hypertension and diabetes was determined, and participants’ knowledge and treatment practices were assessed in the summer. The prevalence of hypertension in the study population was 73.52%, affecting both sexes, with women presenting a higher prevalence. The prevalence of diabetes is slightly higher (75.29%) than that of men (70.79%) was 36.59%, with men (38.05%) being slightly more affected than women (35.63%). Obesity and overweight were contributory important factors, affecting respectively 25.78% and 37.28% of the population. Among the hypertensive people, 84.83% were aware of their condition, but only 7.26% received treatment, with no case-controlled hypertension having no summer observed. Socio-economic factors, including the urban setting and proximity to the capital, have probably contributed to these results. The high prevalence of hypertension and diabetes in Dubréka reflects the broader epidemiological transition in sub-Saharan Africa, with women being disproportionately affected by hypertension linked to obesity. Access limited to health care, especially in terms of treatment and care, further aggravates the burden of these diseases. Public health interventions are urgently needed to improve awareness, treatment accessibility and culturally appropriate management strategies adapted to fight against the growing burden of NCDs in Guinea peri-urban.展开更多
Tigecycline is one of the most critical drugs for treating Gram-negative bacterial infections;however,the emergence of the tigecycline resistance efflux pump TMexCD1-TOprJ1 poses a global health threat.The evolutionar...Tigecycline is one of the most critical drugs for treating Gram-negative bacterial infections;however,the emergence of the tigecycline resistance efflux pump TMexCD1-TOprJ1 poses a global health threat.The evolutionary relationships and epidemiological trends of tmexCD1-toprJ1-positive strains across various ecological niches remain largely unexplored.In this study,we employed whole-genome sequencing(WGS)of tmexCD1-toprJ1-positive bacteria from humans,food,animals,and the environment in China to assess the epidemiological and genomic features of these strains,analyzing both newly collected strains and data from the GenBank database.From 3434 samples collected during 2019-2022,145tmexCD1-toprJ1-carrying strains(4.5%)were isolated.The majority of the tmexCD1-toprJ1-positive Enterobacterales exhibited resistance to nearly all antimicrobials,including colistin(42.13%).tmexCD1-toprJ1 was predominantly identified in Klebsiella pneumoniae(K.pneumoniae)from chicken feces in China but was also detected in multiple ecological niches and other countries.Phylogenetic analysis revealed the clonal transmission of tmexCD1-toprJ1-positive ST37 K.pneumoniae across diverse ecological niches as well as the international spread of the ST15 K.pneumoniae clone-producing TMexCD1-TOprJ1.tmexCD1-toprJ1 is mainly carried by Klebsiella spp.specific narrow host range plasmids,which may limit the spread of tmexCD1-toprJ1 across different bacterial species.Notably,due to the fitness cost posed by tmexCD1-toprJ1,the occurrence of tmexCD1-toprJ1-positive Enterobacterales in both food animals and humans in China has declined significantly following the withdrawal of antibiotics as growth promoters in food animals in China since 2020.However,tmexCD1-toprJ1 has been captured by broad-host-range plasmids and hypervirulent carbapenem-resistant K.pneumoniae ST11-KL64 strains in healthcare settings.The frequent use of tetracyclines in chicken farming likely contributes to the high detection rate of tmexCD1-toprJ1;therefore,to reduce the threat of tmexCD1-toprJ1-positive K.pneumoniae,continuous monitoring of tmexCD1-toprJ1 across different ecological niches and strict enforcement of antimicrobial policies in animal husbandry,particularly in the poultry industry,are urgently required.展开更多
Insects are considered as an important source of essential nutrients because of their nutritional value which in turn is related to proteins, lipids, and mineral elements. In order to optimise the nutritional quality ...Insects are considered as an important source of essential nutrients because of their nutritional value which in turn is related to proteins, lipids, and mineral elements. In order to optimise the nutritional quality of insects, there is a need to identify processing and cooking methods that will result in higher retention of quality nutrients. Several researchers have investigated the effect of cooking methods on the nutritional quality of edible insects. The effect of cooking time on physico-chemical and nutritional parameters of Macrotermes subhyalinus Rambur and Imbrasia obscura Butler was evaluated in this study. M. subhyalinus échantillons were fried and grilled at a temperature of 150°C at intervals of 3, 6, 9, and 12 minutes. I. obscura was initially boiled at 93.4°C for 6, 9, 12, and 15 minutes. Then, I. obscura which had been boiled for six minutes at 93.4°C was fried for 3, 6, 9, and 12 minutes, respectively, at 150°C. The analysis used the pre-levered samples from those various times. The results obtained for M. subhyalinus and I. obscura respectively demonstrate that these two insects comprise primarily proteins (36.83 and 59.04 g/100g DM), lipids (54.24 and 18.67 g/100g DM), and total mineral content (5.87 and 7.82 g/100g DM). With increased cooking time, physical-chemical and nutritional indicators decreased significantly (p 0.05). When the insects were fried and toasted, the total mineral content increased, but only the lipids increased considerably (p 0.05). Fry for 3 to 6 minutes and toast for 3 to 6 minutes are treatments for M. subhyalinus that better conserve nutrients. To preserve the nutritional value, scalding I. obscura for 6 minutes and combining it with frying it for 3 minutes are highly advised.展开更多
The effect of Gurusa, a fermented traditional food on lipid profile and body weight was investigated. Thirty male albino rats weighing between 75 - 115 g assigned into five study groups designated A to E (n = 6) were ...The effect of Gurusa, a fermented traditional food on lipid profile and body weight was investigated. Thirty male albino rats weighing between 75 - 115 g assigned into five study groups designated A to E (n = 6) were fed 20 g of prepared pellets and tap water given ad libitum. The animals were served graded doses of Gurusa. Group A was served 100% rat chow pellets (made of maize bran);groups B, C and D were given pellets of 75% rat chow and 25% Gurusa, 50% rat chow and 50% Gurusa, 25% rat chow and 75% Gurusa respectively while group E was served 100% Gurusa. The lipid profile showed a dose dependent effect of Gurusa administration as one moves down the groups. Group D had a reverse action of group B and showed significant (P < 0.05) increase in TC, TG and HDL compared to groups A and B but a significant (P < 0.05) decrease in LDL compared to group B. Weight changes in group B produced a significant (P < 0.05) increase in MWI and GR compared to the other groups. There was no significant (P ≥ 0.05) difference in MWI and GR of group D when compared with group A. However group C showed significant (P < 0.05) increase in MWI and GR when compared with group A. Gurusa consumption alters both lipid profile and body weight and its combination with maize bran or other foods rich in dietary fibre, have potential to serve therapeutic purposes in the prevention of atherosclerosis and obesity depending on the intended objective.展开更多
Introduction: In Guinea, there is few data on dietary practices socio-economic and clinical characteristics of elderly diabetics and hypertensives. Methods: This was a descriptive cross-sectional study of people aged ...Introduction: In Guinea, there is few data on dietary practices socio-economic and clinical characteristics of elderly diabetics and hypertensives. Methods: This was a descriptive cross-sectional study of people aged 60 years and over, seen in consultation or hospitalised in Conakry University hospitals during the period from 5 September to 15 December 2023 inclusively. Results: Among the 320 subjects included in the study, there were more men (50.6%). The average age was 67 ± 7.69 years;53.43% had hypertension and 46.57% had diabetes. Sex was significantly associated with diabetes (p = 0.035). Women in the study were more affected by diabetes (55.7%), 64.4% of diabetic subjects were married and more than 64% of them had dependent children. 26.2% of diabetics were not employed;85.9% had an income;34.9% had an income of less than one million Guinean francs per month and 74.5% of them had 3 meals a day. Physical activity, 24-hour recall for lunch (p Conclusion: Effective management of diabetes and hypertension in the elderly should necessarily involve nutrition education in hospitals.展开更多
A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are...A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are being generated in our homes and agro-processing industries every day.According to previous statistics,nearly half of the fruits are lost or discarded during the entire processing chain.The concern arises when those wastes and by-products damage the environment and simultaneously cause economic losses.There is a lot of potential in these by-products for reuse in a variety of applications,including the isolation of valuable bioactive ingredients and their application in developing healthy and functional foods.The development of novel techniques for the transformation of these materials into marketable commodities may offer a workable solution to this waste issue while also promoting sustainable economic growth from the bio-economic viewpoint.This approach can manage waste as well as add value to enterprises.The goal of this study is twofold based on this scenario.The first is to present a brief overview of the most significant bioactive substances found in those by-products.The second is to review the current status of their valorization including the trends and techniques,safety assessments,sensory attributes,and challenges.Moreover,specific attention is drawn to the future perspective,and some solutions are discussed in this report.展开更多
Octenyl succinic anhydride （OSA） modified early Indica rice starch was prepared in aqueous slurry systems using response surface methodology. The paste properties of the OSA starch were also investigated. Results in...Octenyl succinic anhydride （OSA） modified early Indica rice starch was prepared in aqueous slurry systems using response surface methodology. The paste properties of the OSA starch were also investigated. Results indicated that the suitable parameters for the preparation of OSA starch from early Indica rice starch were as follows： reaction period 4 h, reaction temperature 33.4℃, pH of reaction system 8.4, concentration of starch slurry 36.8% （in proportion to water, w/w）, amount of OSA 3% （in proportion to starch, w/w）. The degree of substitution was 0.0188 and the reaction efficiency was 81.0%. The results of paste properties showed that with increased OSA modification, the starch derivatives had higher paste clarity, decreased retrogradation and better freeze-thaw stability.展开更多
Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive ...Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive components derived from jujube fruit have significant nutritional and potential biological effects.In this paper,the latest progress in research on major bioactive compounds obtained from jujube is reviewed,and the potential biological functions of jujube fruit resources are discussed.As a dietary supplement,jujube fruit is well recognized as a healthy food which contains a variety of bioactive substances,such as polysaccharides,polyphenols,amino acids,nucleotides,fatty acids,dietary fiber,alkaloids,and other nutrients.These nutrients and non-nutritive phytochemicals obtained from jujube fruit have physiological functions including anticancer,antioxidant,anti-inflammatory,anti-hyperlipidemic,anti-hyperglycemic,immunoregulatory,neuroprotective,sedative,and antiviral functions.Of note is that new constituents,including alkaloids,dietary fiber,and other bioactive substances,as well as the antiviral,hypoglycemic,lipid-lowering,and neuroprotective effects of jujube fruit,are systematically reviewed here for the first time.Meanwhile,problems affecting the exploitation of jujube fruit resources are discussed and further research directions proposed.Therefore,this review provides a useful bibliography for the future development of jujube-based products and the utilization of jujube nutritional components in functional foods.展开更多
Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzyme...Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography（HPLC） analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities（2,2＇-azino-bis-（3-ethylbenzthiazoline-6-sulfonic acid） diammonium salt（ABTS）, 2,2-diphenyl-1-picrylhydrazy（DPPH） radical, and ferric reducing antioxidant power（FRAP）） of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species（ROS） production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in Hep G2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.展开更多
Syzygium cumini(S.cumini)(L.) Skeels(jambolan) is one of the widely used medicinal plants in the treatment of various diseases in particular diabetes.The present review has been primed to describe the existing data on...Syzygium cumini(S.cumini)(L.) Skeels(jambolan) is one of the widely used medicinal plants in the treatment of various diseases in particular diabetes.The present review has been primed to describe the existing data on the information on botany,phytochemical constituents,traditional uses and pharmacological actions of 5.cumini(L.) Skeels(jambolan).Electronic database search was conducted with the search terms of Eugenia jambolana,S.cumini,jambolan,common plum and java plum.The plant has been viewed as an antidiabetic plant since it became commercially available several decades ago.During last four decades,numerous folk medicine and scientific reports on the antidiabetic effects of this plant have been cited in the literature.The plant is rich in compounds containing anthocyanins,glucoside,ellagic acid,isoquercetin,kaemferol and myrecetin.The seeds are claimed to contain alkaloid,jambosine,and glycoside jambolin or antimellin,which halts the diastalic conversion of starch into sugar.The vast number of literatures found in the database revealed that the extracts of different parts of jambolan showed significant pharmacological actions.We suggest that there is a need for further investigation to isolate active principles which confer the pharmacological action.Hence identification of such active compounds is useful for producing safer drugs in the treatment of various ailments including diabetes.展开更多
Clinacanthus nutans Lindau is known as snake grass belonging to the Acanlhaceae family.This plant has diverse and potential medicinal uses in traditional herbal medicine for treating skin rashes,insects and snake bite...Clinacanthus nutans Lindau is known as snake grass belonging to the Acanlhaceae family.This plant has diverse and potential medicinal uses in traditional herbal medicine for treating skin rashes,insects and snake bites,lesions caused by herpes simplex virus,diabetes,and gout in Malaysia.Indonesia.Thailand and China.Phylochemieal investigations documented the varied contents of bioaclive compounds from litis plant namely flavonoids,glycosides,glycoglyeerolipids.cerebrosides and monoacylmonogalatosylglycerol.The pharmacological experiment proved that various types of extracts and pure compounds from this species exhibited a broad range of biological properties such as anti-inflammatory,antiviral,antioxidant,and anti-diabetic activities.The lindings of toxicity study showed that extracts from this plant did not show any toxicity thus it can be used as strong therapeutic agents for specific diseased conditions.However,further experiments on chemical components and their mode of action showing biological activities are required to elucidate the complete phytochemical profile and assess to confirm their suitability tor future drugs.This review summarizes the medicinal uses,phytochemistry and pharmacology of this plant in order to explore its therapeutic potential and gaps necessitating for prospected research work.展开更多
A convenient competitive enzyme-linked immunosorbent assay(ELISA) for ciprofloxacin(CPFX) was developed by using rabbit monoclonal antibodies(RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin(BS...A convenient competitive enzyme-linked immunosorbent assay(ELISA) for ciprofloxacin(CPFX) was developed by using rabbit monoclonal antibodies(RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin(BSA).The indirect competitive ELISA of CPFX had a concentration at 50% inhibition(IC50) of 1.47 ng/ml and a limit of detection(LOD) of 0.095 ng/ml.The mAb exhibited some cross-reactivity,however,not so high with enrofloxacin(28.8%),ofloxacin(13.1%),norfloxacin(11.0%),fleroxacin(22.6%),and pefloxacin(20.4%).And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study.The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products.This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%,with coefficients of variation of less than 12.2%.展开更多
Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for ...Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for 35 d. Of these four doses, UV-C irradiation at 4 and 8 kJ m-2 significantly increased total phenolic contents in present tomato fruit by 21.2 and 20.2%, respectively. Furthermore, UV-C irradiation at 4 or 8 kJ m-2 promoted the accumulation of total flavonoids and increased the antioxidant activity. 2 or 16 kJ m-2 UV-C irradiation also enhanced antioxidant activity, but to a lesser extent. Seven phenolic compounds, viz., gallic acid, （＋）-catechin, chlorogenic acid, cafferic acid, syringic acid, p-coumaric acid, and quercetin in tomato fruit were identified and quantified by HPLC. Gallic acid was the major phenolic compound in tomato fruit and significantly correlated with antioxidant activity. 4 or 8 kJ m-2 UV-C irradiation significantly increased the contents of gallic acid, chlorogenic acid, syringic acid, p-coumaric acid, and quercetin. The optimum dose of UV-C irradiation in terms of increased phenolic compound content and enhanced Antioxidant activity was determined to be 4 or 8 kJ m-2.展开更多
AIM： To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells. METHODS： Bot...AIM： To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells. METHODS： Both dephytinized （by adding an exogenous phytase） and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 too. Mineral cell retention, transport, and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells. RESULTS： Dephytinization of infant cereals significantly increased （P 〈 0.05） the cell uptake efficiency （from 0.66%-6.05% to 3.93%-13%）, retention （from 6.04%-16.68% to 14.75%-20.14%） and transport efficiency （from 0.14%-2.21% to 1.47%-6.02%）, of iron, and the uptake efficiency （from 5.0%-35.4% to 7.3%-41.6%） and retention （from 4.05%-20.53% to 14.45%-61.3%） of zinc, whereas calcium only cell uptake showed a significant increase （P 〈 0.05） after removing phytate from most of the samples analyzed. A positive relationship （P 〈 0.05） between mineral solubility and the cell uptake and transport efficiencies was observed. CONCLUSION： Removing phytate from infant cereals had a beneficial effect on iron and zinc bioavailability when infant cereals were reconstituted with water. Since in developing countries cereal-based complementary foods for infants are usually consumed mixed with water, exogenous phytase additions could improve the nutritional value of this weaning food.展开更多
This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca2＋ content,acti...This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX might be the result of gene expression,which could be stimulated by the Ca^2＋ flowing from the membrane to the cytoplasm upon receiving the wounding signals.展开更多
基金the grants from Hangzhou Global Scientific and Technological Innovation Center of Zhejiang University(KC2021ZY0B0003).
文摘The contamination of Atlantic salmon with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has impeded the development of the cold-chain food industry and posed possible risks to the population.Electron beam(E-beam)irradiation under 2,4,7,and 10 kGy can effectively inactivate SARS-CoV-2 in cold-chain seafood.However,there are few statistics about the quality changes of salmon exposed to these irradiation dosages.This work demonstrated that E-beam irradiation at dosages capable of killing SARS-CoV-2 induced lipid oxidation,decreased vitamin A content,and increased some amino acids and ash content.In addition,irradiation altered the textural features of salmon,such as its hardness,resilience,cohesiveness,and chewiness.The irradiation considerably affected the L*,a*,and b*values of salmon,with the L*value increasing and a*,b*values decreasing.There was no significant difference in the sensory evaluation of control and irradiated salmon.It was shown that irradiation with 2−7 kGy E-beam did not significantly degrade quality.The inactivation of SARS-CoV-2 in salmon is advised at a dose of 2 kGy.
文摘Synthetic color is added to foods to replace natural color lost during processing. It is causes health problems mainly in children that considered Avery venerable group. This study was carried out investigate the effect of using color foods (Color fruit juice for 6 - 12 hr. Tomato ketchup potato chips (TKPC) color sweet and color chocolate at low (15%) and high (30%)) concentration on serum biochemical, WBC (white blood cell)and histopathology of liver and kidney of rats for 13 week. The results indicated that no significant change in body weight gain, serum glucose, HDL and LDL cholesterol. TKPC (30%) showed that significant increase in total cholesterol (TC), triacylglycerol (TG). Low and high concentration of color chocolate and color sweet was exhibited significant increase of TG. The level of ALT and AST was significant increase of rat’s administration color fruit juice (for 12 hr.) and TKPC at 30%. High concentration of color foods and long time administration of color fruit juice showed significant increase in serum creatinine and albumin compared to control group. Both low and high consumed of color foods exhibited significant decrease in liver GSH. High concentration of color foods lead to increase number of WBC as the result to the response of the immune system to the inflammation. Color foods were reveled change in histological structure of liver and kidney. In conclusion, the use synthetic color in various foods has adverse effect on some of biochemical analysis;and the liver and kidney histopathological structure.
文摘CHD （coronary heart disease） is the single most important cause of death and, more importantly, the single biggest cause of premature death in modem, industrialized countries. On the basis of summarized results in respect to assessing file nutritional status and dietary pattern of coronary heart diseased patients, it was concluded that nutritional status of control group was better as compared to diseased group. The dietary pattern of diseased group was similar to control group.
文摘Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might be responsible for the property of invasiveness and/or therapies-resistance.CSCs are a self-renewing,awfully tumorigenic subpopulation of cancer cells,which are notorious for strong chemoresistance and are frequently responsible the aggravated invasion,metastasis,and/or recurrence.Developing targeting therapies against CSCs,therefore,may be deliberated a more encouraging mission for the greater cancer therapy.Innovation for a more potent anti-CSC treatment has been required as soon as possible.Interestingly,vitamin D could modulate the inflammatory condition of the tumor microenvironment(TME)by successfully affecting CSCs,which has an imperative role in determining the malignant phenotype of CSCs.In addition,vitamin D may also contribute to the regulation of the malignant behaviors of CSCs.Consistently,vitamin D could have potential applications for the significant inhibition of several tumor growths within various cancer therapies.The biological significance of vitamin D for CSCs regulation may be involved in the function of APRO family proteins.Therefore,vitamin D could be one of the innovative therapeutic modalities for the development of novel CSCs related tumor therapies.
文摘The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several different bacteria,can actively contribute to the regulation of host metabolism,immunity,and inflammation.Roles of ncRNAs and gut microbiota could significantly interact with each other to regulate the growth of various types of cancer.In particular,a causal relationship among ncRNAs,gut microbiota,and immune cells has been shown for their potential importance in the development of breast cancer.Alteration of ncRNA expression and/or gut microbiota profiles could also influence several intracellular signaling pathways with the function of anti-proliferative(APRO)family proteins associated with the malignancy.Targeting ncRNAs and/or APRO family proteins for the treatment of various cancers has been revealed with novel immune therapies.Here,the most recent studies to underline the key role of ncRNAs,APRO family proteins,and gut microbiota in breast cancer progression have been discussed.For more effective breast cancer therapy,it would be imperative to figure out the collective mechanism of ncRNAs,APRO family proteins,and gut microbiota.
文摘Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and lifestyle-related factors. This study aimed to assess the prevalence of these conditions in the urban commune of Dubréka, in Guinea, and to assess the role of demographic and cultural factors in their incidence and management. A cross-sectional study was conducted with 287 participants (174 women and 113 men) in Dubréka. Data were summer collected on the characteristics of sociodemographics, BMI, blood pressure and fasting blood glucose. The prevalence of hypertension and diabetes was determined, and participants’ knowledge and treatment practices were assessed in the summer. The prevalence of hypertension in the study population was 73.52%, affecting both sexes, with women presenting a higher prevalence. The prevalence of diabetes is slightly higher (75.29%) than that of men (70.79%) was 36.59%, with men (38.05%) being slightly more affected than women (35.63%). Obesity and overweight were contributory important factors, affecting respectively 25.78% and 37.28% of the population. Among the hypertensive people, 84.83% were aware of their condition, but only 7.26% received treatment, with no case-controlled hypertension having no summer observed. Socio-economic factors, including the urban setting and proximity to the capital, have probably contributed to these results. The high prevalence of hypertension and diabetes in Dubréka reflects the broader epidemiological transition in sub-Saharan Africa, with women being disproportionately affected by hypertension linked to obesity. Access limited to health care, especially in terms of treatment and care, further aggravates the burden of these diseases. Public health interventions are urgently needed to improve awareness, treatment accessibility and culturally appropriate management strategies adapted to fight against the growing burden of NCDs in Guinea peri-urban.
基金funded by the Guangdong Major Project of Basic and Applied Basic Research(2020B0301030007)the National Natural Science Foundation of China(32141002)+1 种基金the National Key Research and Development Program of China(2022YFC2303900)the Science and Technology Program of Guangzhou,China(2023A04J0755 and 202201010300)。
文摘Tigecycline is one of the most critical drugs for treating Gram-negative bacterial infections;however,the emergence of the tigecycline resistance efflux pump TMexCD1-TOprJ1 poses a global health threat.The evolutionary relationships and epidemiological trends of tmexCD1-toprJ1-positive strains across various ecological niches remain largely unexplored.In this study,we employed whole-genome sequencing(WGS)of tmexCD1-toprJ1-positive bacteria from humans,food,animals,and the environment in China to assess the epidemiological and genomic features of these strains,analyzing both newly collected strains and data from the GenBank database.From 3434 samples collected during 2019-2022,145tmexCD1-toprJ1-carrying strains(4.5%)were isolated.The majority of the tmexCD1-toprJ1-positive Enterobacterales exhibited resistance to nearly all antimicrobials,including colistin(42.13%).tmexCD1-toprJ1 was predominantly identified in Klebsiella pneumoniae(K.pneumoniae)from chicken feces in China but was also detected in multiple ecological niches and other countries.Phylogenetic analysis revealed the clonal transmission of tmexCD1-toprJ1-positive ST37 K.pneumoniae across diverse ecological niches as well as the international spread of the ST15 K.pneumoniae clone-producing TMexCD1-TOprJ1.tmexCD1-toprJ1 is mainly carried by Klebsiella spp.specific narrow host range plasmids,which may limit the spread of tmexCD1-toprJ1 across different bacterial species.Notably,due to the fitness cost posed by tmexCD1-toprJ1,the occurrence of tmexCD1-toprJ1-positive Enterobacterales in both food animals and humans in China has declined significantly following the withdrawal of antibiotics as growth promoters in food animals in China since 2020.However,tmexCD1-toprJ1 has been captured by broad-host-range plasmids and hypervirulent carbapenem-resistant K.pneumoniae ST11-KL64 strains in healthcare settings.The frequent use of tetracyclines in chicken farming likely contributes to the high detection rate of tmexCD1-toprJ1;therefore,to reduce the threat of tmexCD1-toprJ1-positive K.pneumoniae,continuous monitoring of tmexCD1-toprJ1 across different ecological niches and strict enforcement of antimicrobial policies in animal husbandry,particularly in the poultry industry,are urgently required.
文摘Insects are considered as an important source of essential nutrients because of their nutritional value which in turn is related to proteins, lipids, and mineral elements. In order to optimise the nutritional quality of insects, there is a need to identify processing and cooking methods that will result in higher retention of quality nutrients. Several researchers have investigated the effect of cooking methods on the nutritional quality of edible insects. The effect of cooking time on physico-chemical and nutritional parameters of Macrotermes subhyalinus Rambur and Imbrasia obscura Butler was evaluated in this study. M. subhyalinus échantillons were fried and grilled at a temperature of 150°C at intervals of 3, 6, 9, and 12 minutes. I. obscura was initially boiled at 93.4°C for 6, 9, 12, and 15 minutes. Then, I. obscura which had been boiled for six minutes at 93.4°C was fried for 3, 6, 9, and 12 minutes, respectively, at 150°C. The analysis used the pre-levered samples from those various times. The results obtained for M. subhyalinus and I. obscura respectively demonstrate that these two insects comprise primarily proteins (36.83 and 59.04 g/100g DM), lipids (54.24 and 18.67 g/100g DM), and total mineral content (5.87 and 7.82 g/100g DM). With increased cooking time, physical-chemical and nutritional indicators decreased significantly (p 0.05). When the insects were fried and toasted, the total mineral content increased, but only the lipids increased considerably (p 0.05). Fry for 3 to 6 minutes and toast for 3 to 6 minutes are treatments for M. subhyalinus that better conserve nutrients. To preserve the nutritional value, scalding I. obscura for 6 minutes and combining it with frying it for 3 minutes are highly advised.
文摘The effect of Gurusa, a fermented traditional food on lipid profile and body weight was investigated. Thirty male albino rats weighing between 75 - 115 g assigned into five study groups designated A to E (n = 6) were fed 20 g of prepared pellets and tap water given ad libitum. The animals were served graded doses of Gurusa. Group A was served 100% rat chow pellets (made of maize bran);groups B, C and D were given pellets of 75% rat chow and 25% Gurusa, 50% rat chow and 50% Gurusa, 25% rat chow and 75% Gurusa respectively while group E was served 100% Gurusa. The lipid profile showed a dose dependent effect of Gurusa administration as one moves down the groups. Group D had a reverse action of group B and showed significant (P < 0.05) increase in TC, TG and HDL compared to groups A and B but a significant (P < 0.05) decrease in LDL compared to group B. Weight changes in group B produced a significant (P < 0.05) increase in MWI and GR compared to the other groups. There was no significant (P ≥ 0.05) difference in MWI and GR of group D when compared with group A. However group C showed significant (P < 0.05) increase in MWI and GR when compared with group A. Gurusa consumption alters both lipid profile and body weight and its combination with maize bran or other foods rich in dietary fibre, have potential to serve therapeutic purposes in the prevention of atherosclerosis and obesity depending on the intended objective.
文摘Introduction: In Guinea, there is few data on dietary practices socio-economic and clinical characteristics of elderly diabetics and hypertensives. Methods: This was a descriptive cross-sectional study of people aged 60 years and over, seen in consultation or hospitalised in Conakry University hospitals during the period from 5 September to 15 December 2023 inclusively. Results: Among the 320 subjects included in the study, there were more men (50.6%). The average age was 67 ± 7.69 years;53.43% had hypertension and 46.57% had diabetes. Sex was significantly associated with diabetes (p = 0.035). Women in the study were more affected by diabetes (55.7%), 64.4% of diabetic subjects were married and more than 64% of them had dependent children. 26.2% of diabetics were not employed;85.9% had an income;34.9% had an income of less than one million Guinean francs per month and 74.5% of them had 3 meals a day. Physical activity, 24-hour recall for lunch (p Conclusion: Effective management of diabetes and hypertension in the elderly should necessarily involve nutrition education in hospitals.
文摘A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are being generated in our homes and agro-processing industries every day.According to previous statistics,nearly half of the fruits are lost or discarded during the entire processing chain.The concern arises when those wastes and by-products damage the environment and simultaneously cause economic losses.There is a lot of potential in these by-products for reuse in a variety of applications,including the isolation of valuable bioactive ingredients and their application in developing healthy and functional foods.The development of novel techniques for the transformation of these materials into marketable commodities may offer a workable solution to this waste issue while also promoting sustainable economic growth from the bio-economic viewpoint.This approach can manage waste as well as add value to enterprises.The goal of this study is twofold based on this scenario.The first is to present a brief overview of the most significant bioactive substances found in those by-products.The second is to review the current status of their valorization including the trends and techniques,safety assessments,sensory attributes,and challenges.Moreover,specific attention is drawn to the future perspective,and some solutions are discussed in this report.
基金Project (No. 2003C12009) supported by the Science and TechnologyMinistry of Zhejiang Province, China
文摘Octenyl succinic anhydride （OSA） modified early Indica rice starch was prepared in aqueous slurry systems using response surface methodology. The paste properties of the OSA starch were also investigated. Results indicated that the suitable parameters for the preparation of OSA starch from early Indica rice starch were as follows： reaction period 4 h, reaction temperature 33.4℃, pH of reaction system 8.4, concentration of starch slurry 36.8% （in proportion to water, w/w）, amount of OSA 3% （in proportion to starch, w/w）. The degree of substitution was 0.0188 and the reaction efficiency was 81.0%. The results of paste properties showed that with increased OSA modification, the starch derivatives had higher paste clarity, decreased retrogradation and better freeze-thaw stability.
基金the National Natural Science Foundation of China(No.U1703105)the Zhejiang Provincial Key R&D Program of China(No.2019C02074)。
文摘Jujube(Ziziphus jujuba Mill.),a highly nutritious and functional fruit,is reported to have various health benefits and has been extensively planted worldwide,especially in China.Many studies have shown that bioactive components derived from jujube fruit have significant nutritional and potential biological effects.In this paper,the latest progress in research on major bioactive compounds obtained from jujube is reviewed,and the potential biological functions of jujube fruit resources are discussed.As a dietary supplement,jujube fruit is well recognized as a healthy food which contains a variety of bioactive substances,such as polysaccharides,polyphenols,amino acids,nucleotides,fatty acids,dietary fiber,alkaloids,and other nutrients.These nutrients and non-nutritive phytochemicals obtained from jujube fruit have physiological functions including anticancer,antioxidant,anti-inflammatory,anti-hyperlipidemic,anti-hyperglycemic,immunoregulatory,neuroprotective,sedative,and antiviral functions.Of note is that new constituents,including alkaloids,dietary fiber,and other bioactive substances,as well as the antiviral,hypoglycemic,lipid-lowering,and neuroprotective effects of jujube fruit,are systematically reviewed here for the first time.Meanwhile,problems affecting the exploitation of jujube fruit resources are discussed and further research directions proposed.Therefore,this review provides a useful bibliography for the future development of jujube-based products and the utilization of jujube nutritional components in functional foods.
基金supported by the Beijing Municipal Science and Technology Plan(No.D151100004015002)the Research Foundation of the Education Department of Zhejiang Province(No.Y201328143),China
文摘Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography（HPLC） analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities（2,2＇-azino-bis-（3-ethylbenzthiazoline-6-sulfonic acid） diammonium salt（ABTS）, 2,2-diphenyl-1-picrylhydrazy（DPPH） radical, and ferric reducing antioxidant power（FRAP）） of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species（ROS） production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in Hep G2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.
基金fianancially supported by University Grants Commission,New Delhi[grant No.F.4-2/2006(BSR)/13-98/2008(BSR)]
文摘Syzygium cumini(S.cumini)(L.) Skeels(jambolan) is one of the widely used medicinal plants in the treatment of various diseases in particular diabetes.The present review has been primed to describe the existing data on the information on botany,phytochemical constituents,traditional uses and pharmacological actions of 5.cumini(L.) Skeels(jambolan).Electronic database search was conducted with the search terms of Eugenia jambolana,S.cumini,jambolan,common plum and java plum.The plant has been viewed as an antidiabetic plant since it became commercially available several decades ago.During last four decades,numerous folk medicine and scientific reports on the antidiabetic effects of this plant have been cited in the literature.The plant is rich in compounds containing anthocyanins,glucoside,ellagic acid,isoquercetin,kaemferol and myrecetin.The seeds are claimed to contain alkaloid,jambosine,and glycoside jambolin or antimellin,which halts the diastalic conversion of starch into sugar.The vast number of literatures found in the database revealed that the extracts of different parts of jambolan showed significant pharmacological actions.We suggest that there is a need for further investigation to isolate active principles which confer the pharmacological action.Hence identification of such active compounds is useful for producing safer drugs in the treatment of various ailments including diabetes.
文摘Clinacanthus nutans Lindau is known as snake grass belonging to the Acanlhaceae family.This plant has diverse and potential medicinal uses in traditional herbal medicine for treating skin rashes,insects and snake bites,lesions caused by herpes simplex virus,diabetes,and gout in Malaysia.Indonesia.Thailand and China.Phylochemieal investigations documented the varied contents of bioaclive compounds from litis plant namely flavonoids,glycosides,glycoglyeerolipids.cerebrosides and monoacylmonogalatosylglycerol.The pharmacological experiment proved that various types of extracts and pure compounds from this species exhibited a broad range of biological properties such as anti-inflammatory,antiviral,antioxidant,and anti-diabetic activities.The lindings of toxicity study showed that extracts from this plant did not show any toxicity thus it can be used as strong therapeutic agents for specific diseased conditions.However,further experiments on chemical components and their mode of action showing biological activities are required to elucidate the complete phytochemical profile and assess to confirm their suitability tor future drugs.This review summarizes the medicinal uses,phytochemistry and pharmacology of this plant in order to explore its therapeutic potential and gaps necessitating for prospected research work.
基金Project supported by the National High-Tech R&D Program (863) of China (No. 2007AA10Z436)the Important National Science and Technology Specific Projects of China (No. 2009ZX03012-010B)
文摘A convenient competitive enzyme-linked immunosorbent assay(ELISA) for ciprofloxacin(CPFX) was developed by using rabbit monoclonal antibodies(RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin(BSA).The indirect competitive ELISA of CPFX had a concentration at 50% inhibition(IC50) of 1.47 ng/ml and a limit of detection(LOD) of 0.095 ng/ml.The mAb exhibited some cross-reactivity,however,not so high with enrofloxacin(28.8%),ofloxacin(13.1%),norfloxacin(11.0%),fleroxacin(22.6%),and pefloxacin(20.4%).And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study.The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products.This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%,with coefficients of variation of less than 12.2%.
基金Supported by the National Natural Science Foundation of China (30972036)
文摘Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for 35 d. Of these four doses, UV-C irradiation at 4 and 8 kJ m-2 significantly increased total phenolic contents in present tomato fruit by 21.2 and 20.2%, respectively. Furthermore, UV-C irradiation at 4 or 8 kJ m-2 promoted the accumulation of total flavonoids and increased the antioxidant activity. 2 or 16 kJ m-2 UV-C irradiation also enhanced antioxidant activity, but to a lesser extent. Seven phenolic compounds, viz., gallic acid, （＋）-catechin, chlorogenic acid, cafferic acid, syringic acid, p-coumaric acid, and quercetin in tomato fruit were identified and quantified by HPLC. Gallic acid was the major phenolic compound in tomato fruit and significantly correlated with antioxidant activity. 4 or 8 kJ m-2 UV-C irradiation significantly increased the contents of gallic acid, chlorogenic acid, syringic acid, p-coumaric acid, and quercetin. The optimum dose of UV-C irradiation in terms of increased phenolic compound content and enhanced Antioxidant activity was determined to be 4 or 8 kJ m-2.
基金Supported by Fundación Séneca,0578/PI/07,Consejería de Educación, Ciencia a Investigación de la Comunidad Autónoma de la Región de Murcia,CONSOLIDER FUN-C-FOOD.Nuevos ingredientes funcionales para mejorar la salud
文摘AIM： To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells. METHODS： Both dephytinized （by adding an exogenous phytase） and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 too. Mineral cell retention, transport, and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells. RESULTS： Dephytinization of infant cereals significantly increased （P 〈 0.05） the cell uptake efficiency （from 0.66%-6.05% to 3.93%-13%）, retention （from 6.04%-16.68% to 14.75%-20.14%） and transport efficiency （from 0.14%-2.21% to 1.47%-6.02%）, of iron, and the uptake efficiency （from 5.0%-35.4% to 7.3%-41.6%） and retention （from 4.05%-20.53% to 14.45%-61.3%） of zinc, whereas calcium only cell uptake showed a significant increase （P 〈 0.05） after removing phytate from most of the samples analyzed. A positive relationship （P 〈 0.05） between mineral solubility and the cell uptake and transport efficiencies was observed. CONCLUSION： Removing phytate from infant cereals had a beneficial effect on iron and zinc bioavailability when infant cereals were reconstituted with water. Since in developing countries cereal-based complementary foods for infants are usually consumed mixed with water, exogenous phytase additions could improve the nutritional value of this weaning food.
基金Project (No.30771513) supported by the National Natural Science Foundation of China
文摘This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX might be the result of gene expression,which could be stimulated by the Ca^2＋ flowing from the membrane to the cytoplasm upon receiving the wounding signals.
