With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-leng...With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-lenge,prompting innovation in food processing technologies.This review introduces first the common nutritional challenges in the processing of staple food crops,followed by the comprehensive examination of research aiming to enhance the nutritional quality of staple food crop-based foods through innovative processing technologies,including microwave(MW),pulsed electric field(PEF),ultrasound,modern fer-mentation technology,and enzyme technology.Additionally,soybean processing is used as an example to underscore the importance of integrating innovative processing technologies for optimizing nutrient utilization in staple food crops.Although these innovative processing technologies have demonstrated a significant potential to improve nutrient utilization efficiency and enhance the overall nutritional pro-file of staple food crop-based food products,their current limitations must be acknowledged and addressed in future research.Fortunately,advancements in science and technology will facilitate pro-gress in food processing,enabling both the improvement of existing techniques as well as the develop-ment of entirely novel methodologies.This work aims to enhance the understanding of food practitioners on the way processing technologies may optimize nutrient utilization,thereby fostering innovation in food processing research and synergistic multi-technological strategies,ultimately providing valuable references to address global food security challenges.展开更多
Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the...Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the treatment of gastrointestinal and respiratory issues along with increasing the platelet count in patients suffering from dengue.In this review paper,the nutritional composition and bioactive compounds of guava leaves,including proteins,vitamins,minerals,and polysaccharides,have been reviewed.The methods of extraction of bioactive compounds from guava leaves and their bioactivities,including antioxidant,anti-diabetic,and anti-cancer potential,have been explored.Further,encapsulation techniques for improving the transport of bioactive compounds and living cells into foods have been studied.The application of guava leaves in different food systems such as herbal tea,pork sausage,chocolates,jelly,meals for chickens,functional beverages,and as antibacterial agents in food preservatives has also been investigated.The findings of this review illustrated that the phytochemicals present in guava leaves showed excellent antimicrobial and antioxidant properties.Encapsulation appears to be a promising technique for improving the stability,bioavailability,and controlled release of bioactive chemicals extracted from guava leaves.Different methods for the encapsulation of bioactive compounds employed and applied in distinct food systems showed preservation of phytochemicals,improved bioactivities,and nutritional values.Overall,this review paper demonstrated how guava leaves,an abundant source of bioactive compounds,can be used as a multipurpose component to create functional foods,edible coatings,and active packaging to enhance the nutritional,antioxidant,and antimicrobial properties of various food systems that have qualities that promote health.展开更多
The integration of phytochemistry into forensic science has emerged as a groundbreaking frontier,providing unprecedented insights into nature's secrets through the precise application of phytochemical fingerprinti...The integration of phytochemistry into forensic science has emerged as a groundbreaking frontier,providing unprecedented insights into nature's secrets through the precise application of phytochemical fingerprinting of phytotoxins as a cutting-edge approach.This study explores the dynamic intersection of phytochemistry and forensic science,highlighting how the unique phytochemical profiles of toxic plants and their secondary metabolites,serve as distinctive markers for forensic investigations.By utilizing advanced techniques such as Ultra-High-Performance Liquid Chromatography(UHPLC)and High-Resolution Mass Spectrometry(HRMS),the detection and quantification of plant-derived are made more accurate in forensic contexts.Real-world case studies are presented to demonstrate the critical role of plant toxins in forensic outcomes and legal proceedings.The challenges,potential,and future prospects of integrating phytochemical fingerprinting of plant toxins into forensic science were discussed.This review aims to illuminate phytochemical fingerprinting of plant toxins as a promising tool to enhance the precision and depth of forensic analyses,offering new insights into the complex stories embedded in plant toxins.展开更多
Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leave...Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leaves used as food packaging. In total, two hundred and forty (240) samples composed of Thaumatococcus daniellii and Musa paradisiaca leaves were collected and analyzed. Microbial diversity was assessed using specific medium and biochemical tests. The resistance profile was determined by the Müeller-Hinton agar diffusion method. The resistance (blaSHV, blaIMP, blaTEM) and biofilm formation (pslA, pelA) genes were searched by PCR method. Plant leaves were contaminated by bacterial (68.7%) and fungal (100%) strains. Extreme bacterial (7.1 log10 cfu/cm2) and fungal (3.5 log10 cfu/cm2) loads were obtained on Thaumatococcus daniellii leaves. Bacterial prevalence was 45.1% (S. aureus), 38.8% (E. coli) and 16.1 (P. aeruginosa). In order of decreasing importance, the prevalence of fungal species was 41.1% (A. flavus), 33.1% (A. fumigattus), 13.7% (A. niger) and 12.1% Candida sp. Resistance of E. coli to penicillins ranges from 31.6% to 87.3% and to cephalosporins from 13.3% to 28%. The P. aeruginosa strains were mainly resistant to aztreonam (87.6%). Those of S. aureus showed resistance to tetracycline (67.6), vancomycin (53), erythromycin (44.6) and levofloxacin (32.7). The blaSHV (14.28% to 18.60%) and blaIMP (9.52% to 16.28%) genes were detected in the bacterial strains. P. aeruginosa strains (19.05%) harbored the pslA and pelA genes. The health safety of these biodegradable plant-based packaging contributes to their valorization.展开更多
Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens...Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.展开更多
Food allergens are mainly naturally-occurring proteins with immunoglobulin E(IgE)-binding epitopes.Understanding the structural and immunogenic characteristics of allergenic proteins is essential in assessing whether ...Food allergens are mainly naturally-occurring proteins with immunoglobulin E(IgE)-binding epitopes.Understanding the structural and immunogenic characteristics of allergenic proteins is essential in assessing whether and how food processing techniques reduce allergenicity.We here discuss the impacts of food processing technologies on the modification of physicochemical,structural,and immunogenic properties of allergenic proteins.Detection techniques for characterizing changes in these properties of food allergens are summarized.Food processing helps to reduce allergenicity by aggregating or denaturing proteins,which masks,modifies,or destroys antigenic epitopes,whereas,it cannot eliminate allergenicity completely,and sometimes even improves allergenicity by exposing new epitopes.Moreover,most food processing techniques have been tested on purified food allergens rather than food products due to potential interference of other food components.We provide guidance for further development of processing operations that can decrease the allergenicity of allergenic food proteins without negatively impacting the nutritional profile.展开更多
Glutinous rice(Oryza sativa var.glutinosa)stands out as one of the most popular rice varieties globally,amidst thousands of rice cultivars.Its increasing popularity is attributed to its rich nutritional compositions a...Glutinous rice(Oryza sativa var.glutinosa)stands out as one of the most popular rice varieties globally,amidst thousands of rice cultivars.Its increasing popularity is attributed to its rich nutritional compositions and health benefits.This review aims to summarize the nutritional compositions,volatile compounds,and health benefits of glutinous rice.Further,in-depth studies are necessary to explore the utilization of glutinous rice in enhancing processing technologies and developing new food products.Glutinous rice has been shown to possess numerous health benefits,including antioxidant activity,bioactive compounds,anti-cancer properties,anti-inflammatory effects,anti-diabetic potential,and cholesterol-lowering effects.Besides its nutritional compositions,the major volatile compounds identified in glutinous rice could serve as a functional food for human consumption.Emerging processing technologies related to glutinous rice are elaborated to improve the latest developments for incorporating them into various food products.展开更多
Hyperuricemia,a metabolic disorder related to uric acid metabolism dysregulation,has become a common metabolic disease worldwide,due to changes in lifestyle and dietary structure.In recent years,owing to their high ac...Hyperuricemia,a metabolic disorder related to uric acid metabolism dysregulation,has become a common metabolic disease worldwide,due to changes in lifestyle and dietary structure.In recent years,owing to their high activity and few adverse effects,food-derived active peptides used as functional foods against hyperuricemia have attracted increasing attention.This article aims to focus on the challenge associated with peptide-specific preparation methods development,functional components identification,action mechanism(s)clarification,and bioavailability improvement.The current review proposed recent advances in producing the food-derived peptides with high anti-hyperuricemia activity by protein source screening and matched enzymatic hydrolysis condition adjusting,increased the knowledge about strategies to search antihyperuricemia peptides with definite structure,and emphasized the necessity of combining computer-aided approaches and activity evaluations.In addition,novel action mechanism mediated by gut microbiota was discussed,providing different insights from classical mechanism.Moreover,considering that little attention was paid previously on the structure-activity relationships of anti-hyperuricemia peptides,we collected the sequences from published studies and make a preliminary summary about the structure-activity relationships,which in turn provided guides for enzymatic hydrolysis optimization and bioavailability improvement.Hopefully,this article could promote the development,application and commercialization of food-derived anti-hyperuricemia peptides in the future.展开更多
The study addressed a crucial public health concern of dyslipidemia and its management, with a focus on the Ignace DEEN National Hospital patients, filling a research gap in the Republic of Guinea. It contributed valu...The study addressed a crucial public health concern of dyslipidemia and its management, with a focus on the Ignace DEEN National Hospital patients, filling a research gap in the Republic of Guinea. It contributed valuable epidemiological data on dyslipidemia in the studied population, emphasizing gender, age, and socio-professional categories, which could inform tailored prevention and management strategies in improving the health care of dyslipidemia patients by proposing diets to help these patients better manage their disease and a food system for preventing dyslipidemia. The study focused on the lipid profile of patients seen at the cardiology department at the Ignace DEEN National Hospital Republic of Guinea (Conakry). This was a prospective, descriptive study lasting six (6) months from January 24 to July 23, 2021;aimed at determining the frequency of lipid abnormalities and pathologies related to the lipid profile. A total of 523 people were included in this study with a female predominance of 59.34% compared to 40.66% of males for a ratio of 1.5 in favor of women. The age groups of 45 - 54 and 55 - 65 were the most affected with respective frequencies of 45.42% and 30.03%. The most represented socio-professional category was that of housewives, i.e. 45.78%, followed by traders 18.68%. Our series was dominated by the married couple, i.e. 77.29%. Dyslipidemia constitutes a modifiable cardiovascular risk factor in patients seen in the cardiology department at the Ignace DEEN Hospital;its control must be strict through the use of hygienic and dietary rules and hypolipidemia medications.展开更多
Background Meat originating from the spent hen is an important source of poultry meat production;however,multiple factors cause the decline in the meat quality of spent hens.Chinese herbs have been widely used as medi...Background Meat originating from the spent hen is an important source of poultry meat production;however,multiple factors cause the decline in the meat quality of spent hens.Chinese herbs have been widely used as medi-cine for a long time to prevent diseases and as nutrient supplements to improve the product quality.This experi-ment explored the effects of adding 1.0%Chinese herbal formula(CHF,including 0.30%Leonurus japonicus Houtt.,0.20%Salvia miltiorrhiza Bge.,0.25%Ligustrum lucidum Ait.,and 0.25%Taraxacum mongolicum Hand.-Mazz.)for 120 d to the spent hens’diet through metabolomics,network pharmacology,and microbiome strategies.Results The results indicated that CHF supplementation improved the meat quality by reducing drip loss(P<0.05),b*value(P=0.058),and shear force(P=0.099)and increasing cooked meat percentage(P=0.054)and dry matter(P<0.05)of breast muscle.The addition of CHF improved the nutritional value of breast muscle by increasing(P<0.05)the content of C18:2n-6,n-6/n-3 polyunsaturated fatty acids(PUFA),total PUFA,PUFA-to-saturated fatty acids(SFA)ratio,and hypocholesterolemic-to-hypercholesterolemic ratio,and tending to increase serine content(P=0.069).The targeted metabolomics analysis revealed that the biosynthesis of SFA,linoleic acid metabolism,fatty acid degradation,fatty acid elongation,and fatty acid biosynthesis pathways were enriched by CHF supplementation.Furthermore,the network pharmacology analysis indicated that CHF was closely associated with oxidative stress and lipid metabo-lism.The CHF supplementation increased the glutathione peroxidase level(P<0.05)and upregulated gene expres-sion related to the Nrf2 pathway(including HO-1,P<0.05;Nrf2,P=0.098;CAT,P=0.060;GPX1,P=0.063;and SOD2,P=0.052)and lipid metabolism(including PPARγ,P<0.05;SREBP1,P=0.059;and CPT1A,P=0.058).Additionally,CHF supplementation increased Firmicutes and decreased Bacteroidetes,Spirochaetes,and Synergistetes abundances(P<0.05),which may contribute to better meat quality.Conclusions Our results suggest that CHF supplementation improved the quality and nutritional value of meat,which will provide a theoretical basis for the utilization of CHF as a feed additive in spent hens’diets.展开更多
A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are...A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are being generated in our homes and agro-processing industries every day.According to previous statistics,nearly half of the fruits are lost or discarded during the entire processing chain.The concern arises when those wastes and by-products damage the environment and simultaneously cause economic losses.There is a lot of potential in these by-products for reuse in a variety of applications,including the isolation of valuable bioactive ingredients and their application in developing healthy and functional foods.The development of novel techniques for the transformation of these materials into marketable commodities may offer a workable solution to this waste issue while also promoting sustainable economic growth from the bio-economic viewpoint.This approach can manage waste as well as add value to enterprises.The goal of this study is twofold based on this scenario.The first is to present a brief overview of the most significant bioactive substances found in those by-products.The second is to review the current status of their valorization including the trends and techniques,safety assessments,sensory attributes,and challenges.Moreover,specific attention is drawn to the future perspective,and some solutions are discussed in this report.展开更多
Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might b...Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might be responsible for the property of invasiveness and/or therapies-resistance.CSCs are a self-renewing,awfully tumorigenic subpopulation of cancer cells,which are notorious for strong chemoresistance and are frequently responsible the aggravated invasion,metastasis,and/or recurrence.Developing targeting therapies against CSCs,therefore,may be deliberated a more encouraging mission for the greater cancer therapy.Innovation for a more potent anti-CSC treatment has been required as soon as possible.Interestingly,vitamin D could modulate the inflammatory condition of the tumor microenvironment(TME)by successfully affecting CSCs,which has an imperative role in determining the malignant phenotype of CSCs.In addition,vitamin D may also contribute to the regulation of the malignant behaviors of CSCs.Consistently,vitamin D could have potential applications for the significant inhibition of several tumor growths within various cancer therapies.The biological significance of vitamin D for CSCs regulation may be involved in the function of APRO family proteins.Therefore,vitamin D could be one of the innovative therapeutic modalities for the development of novel CSCs related tumor therapies.展开更多
The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several differen...The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several different bacteria,can actively contribute to the regulation of host metabolism,immunity,and inflammation.Roles of ncRNAs and gut microbiota could significantly interact with each other to regulate the growth of various types of cancer.In particular,a causal relationship among ncRNAs,gut microbiota,and immune cells has been shown for their potential importance in the development of breast cancer.Alteration of ncRNA expression and/or gut microbiota profiles could also influence several intracellular signaling pathways with the function of anti-proliferative(APRO)family proteins associated with the malignancy.Targeting ncRNAs and/or APRO family proteins for the treatment of various cancers has been revealed with novel immune therapies.Here,the most recent studies to underline the key role of ncRNAs,APRO family proteins,and gut microbiota in breast cancer progression have been discussed.For more effective breast cancer therapy,it would be imperative to figure out the collective mechanism of ncRNAs,APRO family proteins,and gut microbiota.展开更多
Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and li...Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and lifestyle-related factors. This study aimed to assess the prevalence of these conditions in the urban commune of Dubréka, in Guinea, and to assess the role of demographic and cultural factors in their incidence and management. A cross-sectional study was conducted with 287 participants (174 women and 113 men) in Dubréka. Data were summer collected on the characteristics of sociodemographics, BMI, blood pressure and fasting blood glucose. The prevalence of hypertension and diabetes was determined, and participants’ knowledge and treatment practices were assessed in the summer. The prevalence of hypertension in the study population was 73.52%, affecting both sexes, with women presenting a higher prevalence. The prevalence of diabetes is slightly higher (75.29%) than that of men (70.79%) was 36.59%, with men (38.05%) being slightly more affected than women (35.63%). Obesity and overweight were contributory important factors, affecting respectively 25.78% and 37.28% of the population. Among the hypertensive people, 84.83% were aware of their condition, but only 7.26% received treatment, with no case-controlled hypertension having no summer observed. Socio-economic factors, including the urban setting and proximity to the capital, have probably contributed to these results. The high prevalence of hypertension and diabetes in Dubréka reflects the broader epidemiological transition in sub-Saharan Africa, with women being disproportionately affected by hypertension linked to obesity. Access limited to health care, especially in terms of treatment and care, further aggravates the burden of these diseases. Public health interventions are urgently needed to improve awareness, treatment accessibility and culturally appropriate management strategies adapted to fight against the growing burden of NCDs in Guinea peri-urban.展开更多
The valorization of vegetable by-products is a promising strategy to combat climate change and achieve global carbon neutrality goals.This study aimed to exploit the high-value utilization of garlic by-products and to...The valorization of vegetable by-products is a promising strategy to combat climate change and achieve global carbon neutrality goals.This study aimed to exploit the high-value utilization of garlic by-products and to investigate the antihypertensive effects and potential mechanisms of the resulting angiotensin I-converting enzyme(ACE)inhibitory peptides.After protein extraction,enzymatic hydrolysis,and activity-directed fractionation,a potent and highly stable ACE inhibitory peptide(IC_(50):31.38μmol/L)was obtained,which was identified as VWAS and acted as a competitive inhibitor.VWAS stably bound to key residues in the ACE active center mainly through hydrogen bonding interactions and effectively lowered blood pressure in spontaneously hypertensive rats via alleviating renal and cardiac injuries,improving endothelial dysfunction,and regulating the renal renin-angiotensin system,antioxidant system,and anti-inflammatory system.These findings suggested that garlic protein-derived peptide(VWAS)was a desirable candidate for antihypertensive functional foods and provided guidance for the high-value utilization of garlic by-products.展开更多
Dietary phosphatidylcholine(PC)is an ideal source of bioactive lipids showing efficacy to alleviate nonalcoholic fatty liver disease(NAFLD).In this study,the lipidomic profiles of nonalcoholic steatosis in mice and He...Dietary phosphatidylcholine(PC)is an ideal source of bioactive lipids showing efficacy to alleviate nonalcoholic fatty liver disease(NAFLD).In this study,the lipidomic profiles of nonalcoholic steatosis in mice and HepG2 cells treated with PC isolated from eggs(EPC)and soybean(SPC)were evaluated.EPC and SPC could significantly ameliorate fatty liver disease by down-regulating triglyceride(TG)and diglyceride(DG)levels.Remarkably,EPC exhibited better performance in regulating the hepatic lipid profile leading to decreased PC and TG,ceramides,phosphatidylethanolamine and phosphatidylserine levels.A total of 117 and 20 lipid biomarkers in liver and HepG2 cells involved in glycerophospholipid metabolism were screened out,respectively.The regulatory mechanism of EPC may be attributed to a decrease in the expression of ACACA and SREBP-1c.EPC,via suppressing lipogenic gene expression,which was superior to that of SPC in alleviating nonalcoholic steatosis.Overall,EPC and SPC attenuated HFD-induced NAFLD by improving the hepatic lipid profile.The more significant effect observed with EPC may be attributed to its modulation of glycerophospholipid metabolism.展开更多
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter...Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.展开更多
Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomar...Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomarein,and possesses multiple health-promoting properties,such as antioxidant,hypoglycemic and vasorelaxant effects.Previous bioactivity investigations majorly focused on C.tinctoria and its crude extract.The aim of the present study was to prepare marein-dominant C.tinctoria flavonoids(CF),further investigate the CF protective effects of liver fibrosis induced by carbon tetrachloride and elucidate the associated molecular mechanisms.Results have demonstrated that CF effectively attenuated hepatofibrogenesis by increasing the activity of glutathione(GSH)and superoxide dismutase(SOD);suppressing the hepatic stellate cell(HSC)activation,inhibiting transforming growth factorβ(TGF-β)activation and the production ofα-smooth muscle actin(α-SMA),alleviating the phosphorylation of extracellular signal-regulated kinases1/2(ERK1/2)and small mothers against decapentaplegic1/2(Smad1/2),thus maintaining the collagen metabolic homeostasis in the liver.Our study revealed that CF possesses an efficacious protective effect against chronic hepatic fibrosis due to their strong inhibitory effects of oxidative stress and chronic inflammation.展开更多
Gastrodia elata Blume has a rich history of use in Asian.However,there is still a need for systematic analysis regarding the relationship and in-depth development between its components and functions.Through comprehen...Gastrodia elata Blume has a rich history of use in Asian.However,there is still a need for systematic analysis regarding the relationship and in-depth development between its components and functions.Through comprehensive analysis utilizing databases such as Pub Med,Web of Science,and Google Scholar,the practical components,biological activities,and product development of G.elata have been investigated.It has been found that the main active components of G.elata,such as gastrodin and G.elata polysaccharides,possess functionalities in improving conditions such as epilepsy,hypertension,and osteoporosis.These active components exhibit phenolic hydroxyl groups,molecular weight,conformation,and spatial arrangement,contributing to their enhanced ability to scavenge free radicals.Additionally,G.elata is known to have organic acids and esters,which exhibit potential immune regulatory and antiviral properties.Compounds with multiple acidic functional groups exhibit even more vigorous activity.Steroidal compounds likeβ-sitosterol,due to their presence of alcohol and ketone groups,along with a multi-ring structure,exhibit superior cholesterol-lowering activity.G.elata has already been recognized in the traditional chinese medicine and food homology list,showcasing its potential for development in line with the health demands of the current era.However,challenges regarding quality evaluation,peculiar odor,and the extraction process of the practical components still require immediate attention.Constructing a robust evaluation system and optimizing product processing techniques will be crucial for the future advancement of the G.elata industry.展开更多
The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on...The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on exploring valuable nutrients derived from Cannabis sativa L.(hemp)seeds,particularly hemp seed proteins.Therefore,this study aimed to investigate the release of anti-inflammatory peptides from Lactobacillus paraplantarum-fermented hemp seed proteins.To confirm the complete hydrolysis of hemp seed proteins during the fermentation process,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was employed.Further,the isolation and purification of peptides were achieved through ultrafiltration.The identity of peptides was nextly established using ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MS).The results revealed a total of 39 identified peptides in fermented hemp seeds,with 9 peptides selected based on their relative quantity.Notably,AAELIGVP(P1),AAVPYPQ(P2),VFPEVAP(P4),DVIGVPLG(P6),and PVPKVL(P9)demonstrated strong anti-inflammatory abilities in lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells.Molecular docking was used to understand the potential anti-inflammatory mechanism of these 5 peptides,and in silico results indicated that P1,P2,P4,P6,and P9 could bind to the active sites of toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),and inhibitor of NF-κB kinase(IKK)with higher binding energies.Overall,these findings indicate that hemp seeds have potential to be a source of bioactive peptides for functional foods with anti-inflammatory properties.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD2100205)the Fujian Province Science and Technology Plan Project,China(2023N3008).
文摘With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-lenge,prompting innovation in food processing technologies.This review introduces first the common nutritional challenges in the processing of staple food crops,followed by the comprehensive examination of research aiming to enhance the nutritional quality of staple food crop-based foods through innovative processing technologies,including microwave(MW),pulsed electric field(PEF),ultrasound,modern fer-mentation technology,and enzyme technology.Additionally,soybean processing is used as an example to underscore the importance of integrating innovative processing technologies for optimizing nutrient utilization in staple food crops.Although these innovative processing technologies have demonstrated a significant potential to improve nutrient utilization efficiency and enhance the overall nutritional pro-file of staple food crop-based food products,their current limitations must be acknowledged and addressed in future research.Fortunately,advancements in science and technology will facilitate pro-gress in food processing,enabling both the improvement of existing techniques as well as the develop-ment of entirely novel methodologies.This work aims to enhance the understanding of food practitioners on the way processing technologies may optimize nutrient utilization,thereby fostering innovation in food processing research and synergistic multi-technological strategies,ultimately providing valuable references to address global food security challenges.
文摘Guava(Psidium guajava L.),a plant belonging to the Myrtaceae family,holds significant medicinal and nutritional values.Leaves of the plant are described to be elliptical with a dark green color and are utilized in the treatment of gastrointestinal and respiratory issues along with increasing the platelet count in patients suffering from dengue.In this review paper,the nutritional composition and bioactive compounds of guava leaves,including proteins,vitamins,minerals,and polysaccharides,have been reviewed.The methods of extraction of bioactive compounds from guava leaves and their bioactivities,including antioxidant,anti-diabetic,and anti-cancer potential,have been explored.Further,encapsulation techniques for improving the transport of bioactive compounds and living cells into foods have been studied.The application of guava leaves in different food systems such as herbal tea,pork sausage,chocolates,jelly,meals for chickens,functional beverages,and as antibacterial agents in food preservatives has also been investigated.The findings of this review illustrated that the phytochemicals present in guava leaves showed excellent antimicrobial and antioxidant properties.Encapsulation appears to be a promising technique for improving the stability,bioavailability,and controlled release of bioactive chemicals extracted from guava leaves.Different methods for the encapsulation of bioactive compounds employed and applied in distinct food systems showed preservation of phytochemicals,improved bioactivities,and nutritional values.Overall,this review paper demonstrated how guava leaves,an abundant source of bioactive compounds,can be used as a multipurpose component to create functional foods,edible coatings,and active packaging to enhance the nutritional,antioxidant,and antimicrobial properties of various food systems that have qualities that promote health.
文摘The integration of phytochemistry into forensic science has emerged as a groundbreaking frontier,providing unprecedented insights into nature's secrets through the precise application of phytochemical fingerprinting of phytotoxins as a cutting-edge approach.This study explores the dynamic intersection of phytochemistry and forensic science,highlighting how the unique phytochemical profiles of toxic plants and their secondary metabolites,serve as distinctive markers for forensic investigations.By utilizing advanced techniques such as Ultra-High-Performance Liquid Chromatography(UHPLC)and High-Resolution Mass Spectrometry(HRMS),the detection and quantification of plant-derived are made more accurate in forensic contexts.Real-world case studies are presented to demonstrate the critical role of plant toxins in forensic outcomes and legal proceedings.The challenges,potential,and future prospects of integrating phytochemical fingerprinting of plant toxins into forensic science were discussed.This review aims to illuminate phytochemical fingerprinting of plant toxins as a promising tool to enhance the precision and depth of forensic analyses,offering new insights into the complex stories embedded in plant toxins.
文摘Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leaves used as food packaging. In total, two hundred and forty (240) samples composed of Thaumatococcus daniellii and Musa paradisiaca leaves were collected and analyzed. Microbial diversity was assessed using specific medium and biochemical tests. The resistance profile was determined by the Müeller-Hinton agar diffusion method. The resistance (blaSHV, blaIMP, blaTEM) and biofilm formation (pslA, pelA) genes were searched by PCR method. Plant leaves were contaminated by bacterial (68.7%) and fungal (100%) strains. Extreme bacterial (7.1 log10 cfu/cm2) and fungal (3.5 log10 cfu/cm2) loads were obtained on Thaumatococcus daniellii leaves. Bacterial prevalence was 45.1% (S. aureus), 38.8% (E. coli) and 16.1 (P. aeruginosa). In order of decreasing importance, the prevalence of fungal species was 41.1% (A. flavus), 33.1% (A. fumigattus), 13.7% (A. niger) and 12.1% Candida sp. Resistance of E. coli to penicillins ranges from 31.6% to 87.3% and to cephalosporins from 13.3% to 28%. The P. aeruginosa strains were mainly resistant to aztreonam (87.6%). Those of S. aureus showed resistance to tetracycline (67.6), vancomycin (53), erythromycin (44.6) and levofloxacin (32.7). The blaSHV (14.28% to 18.60%) and blaIMP (9.52% to 16.28%) genes were detected in the bacterial strains. P. aeruginosa strains (19.05%) harbored the pslA and pelA genes. The health safety of these biodegradable plant-based packaging contributes to their valorization.
基金supported by Brain Korea (BK)21 Plus Project (4299990913942)funded by the Korean Government,Koreathe Collabo Project funded by the Ministry of SMEs and Startups (C1016120-01-02)the National Research Foundation of Korea (NRF) (2018007551)。
文摘Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.
基金supported by the National Natural Science Foundation of China (32102605)the Agricultural Science and Technology Innovation Program under Grant (CAAS-ASTIP-2020IAR)the Earmarked Fund for CARS (CARS-44)。
文摘Food allergens are mainly naturally-occurring proteins with immunoglobulin E(IgE)-binding epitopes.Understanding the structural and immunogenic characteristics of allergenic proteins is essential in assessing whether and how food processing techniques reduce allergenicity.We here discuss the impacts of food processing technologies on the modification of physicochemical,structural,and immunogenic properties of allergenic proteins.Detection techniques for characterizing changes in these properties of food allergens are summarized.Food processing helps to reduce allergenicity by aggregating or denaturing proteins,which masks,modifies,or destroys antigenic epitopes,whereas,it cannot eliminate allergenicity completely,and sometimes even improves allergenicity by exposing new epitopes.Moreover,most food processing techniques have been tested on purified food allergens rather than food products due to potential interference of other food components.We provide guidance for further development of processing operations that can decrease the allergenicity of allergenic food proteins without negatively impacting the nutritional profile.
基金the Ministry of Higher Education,Malaysia for financial support via the Transdisciplinary Research Grant Scheme Project(Grant No.TRGS/1/2020/UPM/02/7)。
文摘Glutinous rice(Oryza sativa var.glutinosa)stands out as one of the most popular rice varieties globally,amidst thousands of rice cultivars.Its increasing popularity is attributed to its rich nutritional compositions and health benefits.This review aims to summarize the nutritional compositions,volatile compounds,and health benefits of glutinous rice.Further,in-depth studies are necessary to explore the utilization of glutinous rice in enhancing processing technologies and developing new food products.Glutinous rice has been shown to possess numerous health benefits,including antioxidant activity,bioactive compounds,anti-cancer properties,anti-inflammatory effects,anti-diabetic potential,and cholesterol-lowering effects.Besides its nutritional compositions,the major volatile compounds identified in glutinous rice could serve as a functional food for human consumption.Emerging processing technologies related to glutinous rice are elaborated to improve the latest developments for incorporating them into various food products.
基金sponsored by the National Natural Science Foundation China(32270115)National Key R&D Program of China(2018YFD0901102)+1 种基金Fundamental Research Funds for the Provincial Universities of Zhejiang(SJLY2021015)K.C.Wong Magna Fund of Ningbo University。
文摘Hyperuricemia,a metabolic disorder related to uric acid metabolism dysregulation,has become a common metabolic disease worldwide,due to changes in lifestyle and dietary structure.In recent years,owing to their high activity and few adverse effects,food-derived active peptides used as functional foods against hyperuricemia have attracted increasing attention.This article aims to focus on the challenge associated with peptide-specific preparation methods development,functional components identification,action mechanism(s)clarification,and bioavailability improvement.The current review proposed recent advances in producing the food-derived peptides with high anti-hyperuricemia activity by protein source screening and matched enzymatic hydrolysis condition adjusting,increased the knowledge about strategies to search antihyperuricemia peptides with definite structure,and emphasized the necessity of combining computer-aided approaches and activity evaluations.In addition,novel action mechanism mediated by gut microbiota was discussed,providing different insights from classical mechanism.Moreover,considering that little attention was paid previously on the structure-activity relationships of anti-hyperuricemia peptides,we collected the sequences from published studies and make a preliminary summary about the structure-activity relationships,which in turn provided guides for enzymatic hydrolysis optimization and bioavailability improvement.Hopefully,this article could promote the development,application and commercialization of food-derived anti-hyperuricemia peptides in the future.
文摘The study addressed a crucial public health concern of dyslipidemia and its management, with a focus on the Ignace DEEN National Hospital patients, filling a research gap in the Republic of Guinea. It contributed valuable epidemiological data on dyslipidemia in the studied population, emphasizing gender, age, and socio-professional categories, which could inform tailored prevention and management strategies in improving the health care of dyslipidemia patients by proposing diets to help these patients better manage their disease and a food system for preventing dyslipidemia. The study focused on the lipid profile of patients seen at the cardiology department at the Ignace DEEN National Hospital Republic of Guinea (Conakry). This was a prospective, descriptive study lasting six (6) months from January 24 to July 23, 2021;aimed at determining the frequency of lipid abnormalities and pathologies related to the lipid profile. A total of 523 people were included in this study with a female predominance of 59.34% compared to 40.66% of males for a ratio of 1.5 in favor of women. The age groups of 45 - 54 and 55 - 65 were the most affected with respective frequencies of 45.42% and 30.03%. The most represented socio-professional category was that of housewives, i.e. 45.78%, followed by traders 18.68%. Our series was dominated by the married couple, i.e. 77.29%. Dyslipidemia constitutes a modifiable cardiovascular risk factor in patients seen in the cardiology department at the Ignace DEEN Hospital;its control must be strict through the use of hygienic and dietary rules and hypolipidemia medications.
基金supported by the National Key Research and Development Project(2022YFC3400700)the City-School Cooperation Project of the Fuyang Science and Technology Special Fund undertaken by Fuyang Normal University(SXHZ2020007)+1 种基金the Basic Research Program of Shenzhen Municipal Government(JCYJ20200109114242138)the Special Commissioner for Rural Science and Technology of Guangdong Province(KTP20210345).
文摘Background Meat originating from the spent hen is an important source of poultry meat production;however,multiple factors cause the decline in the meat quality of spent hens.Chinese herbs have been widely used as medi-cine for a long time to prevent diseases and as nutrient supplements to improve the product quality.This experi-ment explored the effects of adding 1.0%Chinese herbal formula(CHF,including 0.30%Leonurus japonicus Houtt.,0.20%Salvia miltiorrhiza Bge.,0.25%Ligustrum lucidum Ait.,and 0.25%Taraxacum mongolicum Hand.-Mazz.)for 120 d to the spent hens’diet through metabolomics,network pharmacology,and microbiome strategies.Results The results indicated that CHF supplementation improved the meat quality by reducing drip loss(P<0.05),b*value(P=0.058),and shear force(P=0.099)and increasing cooked meat percentage(P=0.054)and dry matter(P<0.05)of breast muscle.The addition of CHF improved the nutritional value of breast muscle by increasing(P<0.05)the content of C18:2n-6,n-6/n-3 polyunsaturated fatty acids(PUFA),total PUFA,PUFA-to-saturated fatty acids(SFA)ratio,and hypocholesterolemic-to-hypercholesterolemic ratio,and tending to increase serine content(P=0.069).The targeted metabolomics analysis revealed that the biosynthesis of SFA,linoleic acid metabolism,fatty acid degradation,fatty acid elongation,and fatty acid biosynthesis pathways were enriched by CHF supplementation.Furthermore,the network pharmacology analysis indicated that CHF was closely associated with oxidative stress and lipid metabo-lism.The CHF supplementation increased the glutathione peroxidase level(P<0.05)and upregulated gene expres-sion related to the Nrf2 pathway(including HO-1,P<0.05;Nrf2,P=0.098;CAT,P=0.060;GPX1,P=0.063;and SOD2,P=0.052)and lipid metabolism(including PPARγ,P<0.05;SREBP1,P=0.059;and CPT1A,P=0.058).Additionally,CHF supplementation increased Firmicutes and decreased Bacteroidetes,Spirochaetes,and Synergistetes abundances(P<0.05),which may contribute to better meat quality.Conclusions Our results suggest that CHF supplementation improved the quality and nutritional value of meat,which will provide a theoretical basis for the utilization of CHF as a feed additive in spent hens’diets.
文摘A significant portion of the human diet is comprised of fruits,which are consumed globally either raw or after being processed.A huge amount of waste and by-products such as skins,seeds,cores,rags,rinds,pomace,etc.are being generated in our homes and agro-processing industries every day.According to previous statistics,nearly half of the fruits are lost or discarded during the entire processing chain.The concern arises when those wastes and by-products damage the environment and simultaneously cause economic losses.There is a lot of potential in these by-products for reuse in a variety of applications,including the isolation of valuable bioactive ingredients and their application in developing healthy and functional foods.The development of novel techniques for the transformation of these materials into marketable commodities may offer a workable solution to this waste issue while also promoting sustainable economic growth from the bio-economic viewpoint.This approach can manage waste as well as add value to enterprises.The goal of this study is twofold based on this scenario.The first is to present a brief overview of the most significant bioactive substances found in those by-products.The second is to review the current status of their valorization including the trends and techniques,safety assessments,sensory attributes,and challenges.Moreover,specific attention is drawn to the future perspective,and some solutions are discussed in this report.
文摘Malignant tumors are heterogeneous diseases characterized by uncontrolled cell proliferation,invasion,metastasis,and/or recurrence of their malignancies.In particular,cancer stem cells(CSCs)within these tumors might be responsible for the property of invasiveness and/or therapies-resistance.CSCs are a self-renewing,awfully tumorigenic subpopulation of cancer cells,which are notorious for strong chemoresistance and are frequently responsible the aggravated invasion,metastasis,and/or recurrence.Developing targeting therapies against CSCs,therefore,may be deliberated a more encouraging mission for the greater cancer therapy.Innovation for a more potent anti-CSC treatment has been required as soon as possible.Interestingly,vitamin D could modulate the inflammatory condition of the tumor microenvironment(TME)by successfully affecting CSCs,which has an imperative role in determining the malignant phenotype of CSCs.In addition,vitamin D may also contribute to the regulation of the malignant behaviors of CSCs.Consistently,vitamin D could have potential applications for the significant inhibition of several tumor growths within various cancer therapies.The biological significance of vitamin D for CSCs regulation may be involved in the function of APRO family proteins.Therefore,vitamin D could be one of the innovative therapeutic modalities for the development of novel CSCs related tumor therapies.
文摘The non-coding RNAs(ncRNAs)are a family of single-stranded RNAs that have become recognized as crucial gene expression regulators in normal and cancer cell biology.The gut microbiota,which consists of several different bacteria,can actively contribute to the regulation of host metabolism,immunity,and inflammation.Roles of ncRNAs and gut microbiota could significantly interact with each other to regulate the growth of various types of cancer.In particular,a causal relationship among ncRNAs,gut microbiota,and immune cells has been shown for their potential importance in the development of breast cancer.Alteration of ncRNA expression and/or gut microbiota profiles could also influence several intracellular signaling pathways with the function of anti-proliferative(APRO)family proteins associated with the malignancy.Targeting ncRNAs and/or APRO family proteins for the treatment of various cancers has been revealed with novel immune therapies.Here,the most recent studies to underline the key role of ncRNAs,APRO family proteins,and gut microbiota in breast cancer progression have been discussed.For more effective breast cancer therapy,it would be imperative to figure out the collective mechanism of ncRNAs,APRO family proteins,and gut microbiota.
文摘Hypertension high blood pressure (HTN) and diabetes Type 2 diabetes mellitus (T2DM) are noncommunicable diseases (NCDs) growing in sub-Saharan Africa, under the effect of urbanization, changes in socio-economic and lifestyle-related factors. This study aimed to assess the prevalence of these conditions in the urban commune of Dubréka, in Guinea, and to assess the role of demographic and cultural factors in their incidence and management. A cross-sectional study was conducted with 287 participants (174 women and 113 men) in Dubréka. Data were summer collected on the characteristics of sociodemographics, BMI, blood pressure and fasting blood glucose. The prevalence of hypertension and diabetes was determined, and participants’ knowledge and treatment practices were assessed in the summer. The prevalence of hypertension in the study population was 73.52%, affecting both sexes, with women presenting a higher prevalence. The prevalence of diabetes is slightly higher (75.29%) than that of men (70.79%) was 36.59%, with men (38.05%) being slightly more affected than women (35.63%). Obesity and overweight were contributory important factors, affecting respectively 25.78% and 37.28% of the population. Among the hypertensive people, 84.83% were aware of their condition, but only 7.26% received treatment, with no case-controlled hypertension having no summer observed. Socio-economic factors, including the urban setting and proximity to the capital, have probably contributed to these results. The high prevalence of hypertension and diabetes in Dubréka reflects the broader epidemiological transition in sub-Saharan Africa, with women being disproportionately affected by hypertension linked to obesity. Access limited to health care, especially in terms of treatment and care, further aggravates the burden of these diseases. Public health interventions are urgently needed to improve awareness, treatment accessibility and culturally appropriate management strategies adapted to fight against the growing burden of NCDs in Guinea peri-urban.
基金supported by the Special Fund for Leading Talent in Mount Tai Industry of Shandong Province(TSCY20200121).
文摘The valorization of vegetable by-products is a promising strategy to combat climate change and achieve global carbon neutrality goals.This study aimed to exploit the high-value utilization of garlic by-products and to investigate the antihypertensive effects and potential mechanisms of the resulting angiotensin I-converting enzyme(ACE)inhibitory peptides.After protein extraction,enzymatic hydrolysis,and activity-directed fractionation,a potent and highly stable ACE inhibitory peptide(IC_(50):31.38μmol/L)was obtained,which was identified as VWAS and acted as a competitive inhibitor.VWAS stably bound to key residues in the ACE active center mainly through hydrogen bonding interactions and effectively lowered blood pressure in spontaneously hypertensive rats via alleviating renal and cardiac injuries,improving endothelial dysfunction,and regulating the renal renin-angiotensin system,antioxidant system,and anti-inflammatory system.These findings suggested that garlic protein-derived peptide(VWAS)was a desirable candidate for antihypertensive functional foods and provided guidance for the high-value utilization of garlic by-products.
基金supported by the Shanxi Provincial General Program for Basic Research Project(202303021211093)the National Natural Science Foundation of China(31960492)+1 种基金Key R&D projects in Shanxi Province(202102140601017)Shanxi Agricultural University High-Level Talent Project(2021XG013)。
文摘Dietary phosphatidylcholine(PC)is an ideal source of bioactive lipids showing efficacy to alleviate nonalcoholic fatty liver disease(NAFLD).In this study,the lipidomic profiles of nonalcoholic steatosis in mice and HepG2 cells treated with PC isolated from eggs(EPC)and soybean(SPC)were evaluated.EPC and SPC could significantly ameliorate fatty liver disease by down-regulating triglyceride(TG)and diglyceride(DG)levels.Remarkably,EPC exhibited better performance in regulating the hepatic lipid profile leading to decreased PC and TG,ceramides,phosphatidylethanolamine and phosphatidylserine levels.A total of 117 and 20 lipid biomarkers in liver and HepG2 cells involved in glycerophospholipid metabolism were screened out,respectively.The regulatory mechanism of EPC may be attributed to a decrease in the expression of ACACA and SREBP-1c.EPC,via suppressing lipogenic gene expression,which was superior to that of SPC in alleviating nonalcoholic steatosis.Overall,EPC and SPC attenuated HFD-induced NAFLD by improving the hepatic lipid profile.The more significant effect observed with EPC may be attributed to its modulation of glycerophospholipid metabolism.
基金supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。
文摘Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.
基金funded by the grant for Evaluation of Functional Property,Processing and Utilization of Xinjiang Characteristic Plant Resources(G2023046003L)the Xinjiang Uygur Autonomous Region Outstanding Youth Science Fund project(2024D01E11)+1 种基金National Nature Science Fund of China(82060788)Hebei Natural Science Foundation(C2022204167).
文摘Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomarein,and possesses multiple health-promoting properties,such as antioxidant,hypoglycemic and vasorelaxant effects.Previous bioactivity investigations majorly focused on C.tinctoria and its crude extract.The aim of the present study was to prepare marein-dominant C.tinctoria flavonoids(CF),further investigate the CF protective effects of liver fibrosis induced by carbon tetrachloride and elucidate the associated molecular mechanisms.Results have demonstrated that CF effectively attenuated hepatofibrogenesis by increasing the activity of glutathione(GSH)and superoxide dismutase(SOD);suppressing the hepatic stellate cell(HSC)activation,inhibiting transforming growth factorβ(TGF-β)activation and the production ofα-smooth muscle actin(α-SMA),alleviating the phosphorylation of extracellular signal-regulated kinases1/2(ERK1/2)and small mothers against decapentaplegic1/2(Smad1/2),thus maintaining the collagen metabolic homeostasis in the liver.Our study revealed that CF possesses an efficacious protective effect against chronic hepatic fibrosis due to their strong inhibitory effects of oxidative stress and chronic inflammation.
基金supported by the Jilin Scientific and Technological Development Program(20230202047NC)。
文摘Gastrodia elata Blume has a rich history of use in Asian.However,there is still a need for systematic analysis regarding the relationship and in-depth development between its components and functions.Through comprehensive analysis utilizing databases such as Pub Med,Web of Science,and Google Scholar,the practical components,biological activities,and product development of G.elata have been investigated.It has been found that the main active components of G.elata,such as gastrodin and G.elata polysaccharides,possess functionalities in improving conditions such as epilepsy,hypertension,and osteoporosis.These active components exhibit phenolic hydroxyl groups,molecular weight,conformation,and spatial arrangement,contributing to their enhanced ability to scavenge free radicals.Additionally,G.elata is known to have organic acids and esters,which exhibit potential immune regulatory and antiviral properties.Compounds with multiple acidic functional groups exhibit even more vigorous activity.Steroidal compounds likeβ-sitosterol,due to their presence of alcohol and ketone groups,along with a multi-ring structure,exhibit superior cholesterol-lowering activity.G.elata has already been recognized in the traditional chinese medicine and food homology list,showcasing its potential for development in line with the health demands of the current era.However,challenges regarding quality evaluation,peculiar odor,and the extraction process of the practical components still require immediate attention.Constructing a robust evaluation system and optimizing product processing techniques will be crucial for the future advancement of the G.elata industry.
基金the 4^(th) Brain Korea(BK)21 Plus Project(4299990913942)financed by the Korean Government,Republic of Korea.
文摘The inflammatory response is a crucial physiological process that can lead to tissue damage and is considered a causative factor for various chronic diseases,such as rheumatoid arthritis.Recent research has focused on exploring valuable nutrients derived from Cannabis sativa L.(hemp)seeds,particularly hemp seed proteins.Therefore,this study aimed to investigate the release of anti-inflammatory peptides from Lactobacillus paraplantarum-fermented hemp seed proteins.To confirm the complete hydrolysis of hemp seed proteins during the fermentation process,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was employed.Further,the isolation and purification of peptides were achieved through ultrafiltration.The identity of peptides was nextly established using ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MS).The results revealed a total of 39 identified peptides in fermented hemp seeds,with 9 peptides selected based on their relative quantity.Notably,AAELIGVP(P1),AAVPYPQ(P2),VFPEVAP(P4),DVIGVPLG(P6),and PVPKVL(P9)demonstrated strong anti-inflammatory abilities in lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells.Molecular docking was used to understand the potential anti-inflammatory mechanism of these 5 peptides,and in silico results indicated that P1,P2,P4,P6,and P9 could bind to the active sites of toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),and inhibitor of NF-κB kinase(IKK)with higher binding energies.Overall,these findings indicate that hemp seeds have potential to be a source of bioactive peptides for functional foods with anti-inflammatory properties.
