Objective： To compare the survival outcomes of transabdominal （TA） and transthoracic （TT） surgical approaches in patients with Siewert-II/III esophagogastric junction adenocarcinoma. Methods： This retrospective ...Objective： To compare the survival outcomes of transabdominal （TA） and transthoracic （TT） surgical approaches in patients with Siewert-II/III esophagogastric junction adenocarcinoma. Methods： This retrospective study was conducted in patients with Siewert-II/III esophagogastric junction adenocarcinoma who underwent either TT or TA operations in the West China Hospital between January 2006 and December 2009. Results： A total of 308 patients （109 in the TT and 199 in the TA groups） were included in this study with a follow-up rate of 87.3%. The median （P25, P75） number of harvested perigastric lymph nodes was 8 （5, 10） in the TT group and 23 （16, 34） in the TA group （P〈0.001）, and the number of positive perigastric lymph nodes was 2 （0, 5） in the TT group and 3 （1, 8） in the TA group （P〈0.004）. The 5-year overall survival （OS） rate was 36% in the TT group and 51% in the TA group （P=0.005）. Subgroup analysis by Siewert classification showed that 5-year OS rates for patients with Siewert II tumors were 38% and 48% in TT and TA groups, respectively （P=0.134）, whereas the 5-year OS rate for patients with Siewert III tumors was significantly lower in the TT group than that in the TA group （33% vs. 53%; P=0.010）. Multivariate analysis indicated that N2 and N3 stages, RI/R2 resection and a TT surgical approach were prognostic factors for poor OS. Conclusions： Improved perigastric lymph node dissection may be the main reason for better survival outcomes observed with a TA gastrectomy approach than with TT gastrectomy for Siewert III tumor patients.展开更多
The anti-bird-strike performance of a lattice-material-infilled curved plate is investigated herein.Since automatically filling the curved structure by classical lattice material filling methods will cause a large num...The anti-bird-strike performance of a lattice-material-infilled curved plate is investigated herein.Since automatically filling the curved structure by classical lattice material filling methods will cause a large number of manufacturing defects,a space-dependent lattice material filling method for the curved plate is firstly proposed in this paper Next,using a face-centered cubic lattice,a lattice-material-infilled test piece with a hollow ratio of 40.8%is built.The test pieces are manufactured via additive manufacturing using titanium alloy.In bird-strike experimental tests,the test pieces are crashed against gelatin birds at an impact velocity of 200 m/s.Dynamic strain gauges are used to record the crash history and the results are discussed.Furthermore,a numerical analysis to simulate the bird-strike experiment is performed.The results from the experimental tests and numerical simulation agree well.This work shows that the lattice-material-infilled curved plate yields promising bird-strike resistance.Therefore,lattice-infilled materials are feasible for protecting aerospace components against bird-strike as well as for reducing the component weight.展开更多
Objectives:This study aimed to develop and validate the Nurse’sWorkplace Mental Health Questionnaire(NWMHQ).Methods:The questionnaire was developed based on the two continua model of mental illness and health propose...Objectives:This study aimed to develop and validate the Nurse’sWorkplace Mental Health Questionnaire(NWMHQ).Methods:The questionnaire was developed based on the two continua model of mental illness and health proposed by Keyes.The initial questionnaire was generated through literature review,two rounds of Delphi expert consultation,followed by a pilot survey.Finally,the reliability and validity of the questionnaire were validated through an online survey of 2,815 registered nurses selected from the public hospitals in 11 provinces from June to July 2020.Results:The item-content validity index(I-CVI)of the questionnaire ranged from 0.750 to 1.000 and the average scale-level content validity index(S-CVI/Ave)was 0.906.Cronbach’s a coefficient was 0.948 and test-retest reliability was 0.850.The self-rating depression scale score was negatively related to the NWMHQ score(r=0.664,P<0.01).The exploratory factor analysis(EFA)yielded six factors(emotional status,psychological security,positive relationship,resilience,self-efficacy,and subjective well-being),consisting of 32 items.The cumulative variance contribution rate was 65.58%.Confirmatory factor analysis(CFA)showed an acceptable fit.Conclusion:The NWMHQ developed in this study showed good reliability and validity.This questionnaire may help assess the mental health status of nurses and help nursing managers to develop appropriate targeted psychological interventions.展开更多
The friction sheets working process was analyzed. It is found that its characteristic is microregion instantaneous high temperature and the current cooling method, making the sheets cooled by the lubricating oil flowi...The friction sheets working process was analyzed. It is found that its characteristic is microregion instantaneous high temperature and the current cooling method, making the sheets cooled by the lubricating oil flowing through the friction surface, is not very efficient. Then, intelligent materials concept was introduced, the component and microstructure of intelligent Cu-based friction materials were designed, and the intelligent Cu-based wet friction materials as well as sheets were manufactured. And the intelligent friction materials working principle, i.e. the materials cooling the friction microregion in real time or the friction sheets cutting the peak value of microregion instantaneous high temperature during friction process, was given depending on the characteristics of the materials’ and friction sheets’ working process. Finally, it is indicated that the intelligent friction sheets excell the currently used friction sheets in properties, including anti-heating property, anti-wearing property as well as friction characteristic.展开更多
We are in an era of technological revolutions promoting personalised healthcare.Advances in medical imaging techniques with 3D imaging software and 3D printing have allowed healthcare professionals to view and documen...We are in an era of technological revolutions promoting personalised healthcare.Advances in medical imaging techniques with 3D imaging software and 3D printing have allowed healthcare professionals to view and document various geometrical structures in a brand-new way,enabling them to make meaningful 3D measurements more accurately by generating both virtual and physical models used for preoperative planning,physician-patient communication,and fabrication of surgical guides,instruments,and implants[1-5].With improvements in cost-effectiveness,efficiency,and mechanical properties,3D printing technologies have become a powerful tool for physicians to meet clinical requirements.展开更多
Background:Knee osteoarthritis(OA)is still challenging to prevent or treat.Enhanced endoplasmic reticulum(ER)stress and increased pyroptosis in chondrocytes may be responsible for cartilage degeneration.This study aim...Background:Knee osteoarthritis(OA)is still challenging to prevent or treat.Enhanced endoplasmic reticulum(ER)stress and increased pyroptosis in chondrocytes may be responsible for cartilage degeneration.This study aims to investigate the effect of ER stress on chondrocyte pyroptosis and the upstream regulatory mechanisms,which have rarely been reported.Methods:The expression of the histone methyltransferase enhancer of zeste homolog 2(EZH2),microRNA-142-3p(miR-142-3p),and high mobility group box 1(HMGB1)and the levels of ER stress,pyroptosis,and metabolic markers in normal and OA chondrocytes were investigated by western blotting,quantitative polymerase chain reaction,immunohistochemistry,fluorescence in situ hybridization,fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone(FAM-YVAD-FMK)/Hoechst 33342/propidium iodide(PI)staining,lactate dehydrogenase(LDH)release assays,and cell viability assessments.The effects of EZH2,miR-142-3p,and HMGB1 on ER stress and pyroptosis and the hierarchical regulatory relationship between them were analyzed by chromatin immunoprecipitation,luciferase reporters,gain/loss-of-function assays,and rescue assays in interleukin(IL)-1β-induced OA chondrocytes.The mechanistic contribution of EZH2,miR-142-3p,and HMGB1 to chondrocyte ER stress and pyroptosis and therapeutic prospects were validated radiologically,histologically,and immunohistochemically in surgically induced OA rats.Results:Increased EZH2 and HMGB1,decreased miR-142-3p,enhanced ER stress,and activated pyroptosis in chondrocytes were associated with OA occurrence and progression.EZH2 and HMGB1 exacerbated and miR-142-3p alleviated ER stress and pyroptosis in OA chondrocytes.EZH2 transcriptionally silenced miR-142-3p via H3K27 trimethylation,and miR-142-3p posttranscriptionally silenced HMGB1 by targeting the 3′-UTR of the HMGB1 gene.Moreover,ER stress mediated the effects of EZH2,miR-142-3p,and HMGB1 on chondrocyte pyroptosis.In vivo experiments mechanistically validated the hierarchical regulatory relationship between EZH2,miR-142-3p,and HMGB1 and their effects on chondrocyte ER stress and pyroptosis.Conclusions:A novel EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis and cartilage degeneration by regulating ER stress in OA,contributing novel mechanistic insights into OA pathogenesis and providing potential targets for future therapeutic research.展开更多
基金supported by National Natural Science Foundation of China(No.81372344)
文摘Objective： To compare the survival outcomes of transabdominal （TA） and transthoracic （TT） surgical approaches in patients with Siewert-II/III esophagogastric junction adenocarcinoma. Methods： This retrospective study was conducted in patients with Siewert-II/III esophagogastric junction adenocarcinoma who underwent either TT or TA operations in the West China Hospital between January 2006 and December 2009. Results： A total of 308 patients （109 in the TT and 199 in the TA groups） were included in this study with a follow-up rate of 87.3%. The median （P25, P75） number of harvested perigastric lymph nodes was 8 （5, 10） in the TT group and 23 （16, 34） in the TA group （P〈0.001）, and the number of positive perigastric lymph nodes was 2 （0, 5） in the TT group and 3 （1, 8） in the TA group （P〈0.004）. The 5-year overall survival （OS） rate was 36% in the TT group and 51% in the TA group （P=0.005）. Subgroup analysis by Siewert classification showed that 5-year OS rates for patients with Siewert II tumors were 38% and 48% in TT and TA groups, respectively （P=0.134）, whereas the 5-year OS rate for patients with Siewert III tumors was significantly lower in the TT group than that in the TA group （33% vs. 53%; P=0.010）. Multivariate analysis indicated that N2 and N3 stages, RI/R2 resection and a TT surgical approach were prognostic factors for poor OS. Conclusions： Improved perigastric lymph node dissection may be the main reason for better survival outcomes observed with a TA gastrectomy approach than with TT gastrectomy for Siewert III tumor patients.
基金provided by National Key R&D Program of China(2018YFB1106400)National Natural Science Foundation of China(11672057,11702052,U1906233)+1 种基金Aeronautical Science Foundation of China(2018ZB63002)China Postdoctoral Science Foundation(2018M640251,2019T120201)。
文摘The anti-bird-strike performance of a lattice-material-infilled curved plate is investigated herein.Since automatically filling the curved structure by classical lattice material filling methods will cause a large number of manufacturing defects,a space-dependent lattice material filling method for the curved plate is firstly proposed in this paper Next,using a face-centered cubic lattice,a lattice-material-infilled test piece with a hollow ratio of 40.8%is built.The test pieces are manufactured via additive manufacturing using titanium alloy.In bird-strike experimental tests,the test pieces are crashed against gelatin birds at an impact velocity of 200 m/s.Dynamic strain gauges are used to record the crash history and the results are discussed.Furthermore,a numerical analysis to simulate the bird-strike experiment is performed.The results from the experimental tests and numerical simulation agree well.This work shows that the lattice-material-infilled curved plate yields promising bird-strike resistance.Therefore,lattice-infilled materials are feasible for protecting aerospace components against bird-strike as well as for reducing the component weight.
基金This study was funded by the Medical Scientific Research Foundation of Guangdong Province,China(No.C2019046)the Guangdong Health Economics Association(No.2020-WJMZ-23).
文摘Objectives:This study aimed to develop and validate the Nurse’sWorkplace Mental Health Questionnaire(NWMHQ).Methods:The questionnaire was developed based on the two continua model of mental illness and health proposed by Keyes.The initial questionnaire was generated through literature review,two rounds of Delphi expert consultation,followed by a pilot survey.Finally,the reliability and validity of the questionnaire were validated through an online survey of 2,815 registered nurses selected from the public hospitals in 11 provinces from June to July 2020.Results:The item-content validity index(I-CVI)of the questionnaire ranged from 0.750 to 1.000 and the average scale-level content validity index(S-CVI/Ave)was 0.906.Cronbach’s a coefficient was 0.948 and test-retest reliability was 0.850.The self-rating depression scale score was negatively related to the NWMHQ score(r=0.664,P<0.01).The exploratory factor analysis(EFA)yielded six factors(emotional status,psychological security,positive relationship,resilience,self-efficacy,and subjective well-being),consisting of 32 items.The cumulative variance contribution rate was 65.58%.Confirmatory factor analysis(CFA)showed an acceptable fit.Conclusion:The NWMHQ developed in this study showed good reliability and validity.This questionnaire may help assess the mental health status of nurses and help nursing managers to develop appropriate targeted psychological interventions.
文摘The friction sheets working process was analyzed. It is found that its characteristic is microregion instantaneous high temperature and the current cooling method, making the sheets cooled by the lubricating oil flowing through the friction surface, is not very efficient. Then, intelligent materials concept was introduced, the component and microstructure of intelligent Cu-based friction materials were designed, and the intelligent Cu-based wet friction materials as well as sheets were manufactured. And the intelligent friction materials working principle, i.e. the materials cooling the friction microregion in real time or the friction sheets cutting the peak value of microregion instantaneous high temperature during friction process, was given depending on the characteristics of the materials’ and friction sheets’ working process. Finally, it is indicated that the intelligent friction sheets excell the currently used friction sheets in properties, including anti-heating property, anti-wearing property as well as friction characteristic.
文摘We are in an era of technological revolutions promoting personalised healthcare.Advances in medical imaging techniques with 3D imaging software and 3D printing have allowed healthcare professionals to view and document various geometrical structures in a brand-new way,enabling them to make meaningful 3D measurements more accurately by generating both virtual and physical models used for preoperative planning,physician-patient communication,and fabrication of surgical guides,instruments,and implants[1-5].With improvements in cost-effectiveness,efficiency,and mechanical properties,3D printing technologies have become a powerful tool for physicians to meet clinical requirements.
基金supported by the National Natural Science Foundation of China(No.81802210)the Science&Technology Department of Sichuan Province(No.2021YFS0122)+1 种基金the Health Commission of Sichuan Province(No.20PJ056)the National Clinical Research Center for Geriatrics,West China Hospital,Sichuan University(No.Z2018B20).
文摘Background:Knee osteoarthritis(OA)is still challenging to prevent or treat.Enhanced endoplasmic reticulum(ER)stress and increased pyroptosis in chondrocytes may be responsible for cartilage degeneration.This study aims to investigate the effect of ER stress on chondrocyte pyroptosis and the upstream regulatory mechanisms,which have rarely been reported.Methods:The expression of the histone methyltransferase enhancer of zeste homolog 2(EZH2),microRNA-142-3p(miR-142-3p),and high mobility group box 1(HMGB1)and the levels of ER stress,pyroptosis,and metabolic markers in normal and OA chondrocytes were investigated by western blotting,quantitative polymerase chain reaction,immunohistochemistry,fluorescence in situ hybridization,fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone(FAM-YVAD-FMK)/Hoechst 33342/propidium iodide(PI)staining,lactate dehydrogenase(LDH)release assays,and cell viability assessments.The effects of EZH2,miR-142-3p,and HMGB1 on ER stress and pyroptosis and the hierarchical regulatory relationship between them were analyzed by chromatin immunoprecipitation,luciferase reporters,gain/loss-of-function assays,and rescue assays in interleukin(IL)-1β-induced OA chondrocytes.The mechanistic contribution of EZH2,miR-142-3p,and HMGB1 to chondrocyte ER stress and pyroptosis and therapeutic prospects were validated radiologically,histologically,and immunohistochemically in surgically induced OA rats.Results:Increased EZH2 and HMGB1,decreased miR-142-3p,enhanced ER stress,and activated pyroptosis in chondrocytes were associated with OA occurrence and progression.EZH2 and HMGB1 exacerbated and miR-142-3p alleviated ER stress and pyroptosis in OA chondrocytes.EZH2 transcriptionally silenced miR-142-3p via H3K27 trimethylation,and miR-142-3p posttranscriptionally silenced HMGB1 by targeting the 3′-UTR of the HMGB1 gene.Moreover,ER stress mediated the effects of EZH2,miR-142-3p,and HMGB1 on chondrocyte pyroptosis.In vivo experiments mechanistically validated the hierarchical regulatory relationship between EZH2,miR-142-3p,and HMGB1 and their effects on chondrocyte ER stress and pyroptosis.Conclusions:A novel EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis and cartilage degeneration by regulating ER stress in OA,contributing novel mechanistic insights into OA pathogenesis and providing potential targets for future therapeutic research.
