Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus...Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus(HR-HPV)infection.Methods Female patients(n=73)with HR-HPV infection were enrolled and divided into a high-grade squamous intraepithelial lesion(HSIL)group(n=33)and a non-HSIL(N-HSIL)group(n=40),which include low-grade squamous intraepithelial lesions and inflammation.Healthy screening subjects(n=31)with negative HR-HPV results were enrolled as a control group.We examined contemporaneous plasma and secretory cytokines from 25 study subjects to investigate the difference between systemic cytokine profiles and the local microenvironment immunity using the Wilcoxon matched-pairs signed rank test.The 12 cytokines from cervical secretions were compared between the three groups using the Mann-Whitney test,and logistic regression was used to analyze HSIL and N-HSIL.Results There were statistical differences in eight cytokines(IL-2,IL-6,TNF-α,IFN-γ,IL-1β,IL-12p70,IFN-α,and IL-8)between cervical secretion and plasma of the same patient,and seven cytokines were statistically different between the control and other two groups.We selected four independent variables(TNF-α,IFN-γ,IL-12p70,and IFN-α)commonly identified by univariate regression analysis and non-parametric tests for multivariate logistic regression analysis.Based on this model,HSIL could be predicted in patients with HR-HPV infection,with the area under the curve being 0.76.Conclusion The systemic cytokine profile cannot reflect the local microenvironment immunity,and the occurrence of HSIL is related to the cytokine levels in the cervical microenvironment.展开更多
Objective:Villoglandular papillary adenocarcinoma(VGPA)of the uterine cervix is a subtype of cervical adenocarcinoma.In the present study,we summarized the clinical features of VGPA of the uterine cervix and discussed...Objective:Villoglandular papillary adenocarcinoma(VGPA)of the uterine cervix is a subtype of cervical adenocarcinoma.In the present study,we summarized the clinical features of VGPA of the uterine cervix and discussed the potential indications for a conservative treatment.Methods:A retrospective review of clinical characteristics and treatment aspects of 10patients with VGPA at the Obstetrics and Gynecology Hospital of Fudan University was conducted between January 2007 and December 2016.Almost all of the existing 40 English papers on“villoglandular papillary adenocarcinoma[title/abstract]”identified from PubMed were obtained.Clinical data from these papers were analyzed in terms of age,International Federation of Gynecology and Obstetrics(FIGO)stage,recurrence rate,mortality,and conservation treatment aspects.Results:The median age of 10patients with VGPA was 40years.All cases had Stage IB 1 disease.Seven patients underwent human papillomavirus examinations,which revealed 6 positive and 1 negative case(s)of infection.Six patients underwent ThinPrep cytologic tests,which revealed 4patients with atypical glandular cells,1 with a high-grade squamous intraepithelial lesion,and 1 who tested negative for intraepithelial malignancy.None of the patients had lymph node metastases.During the 6-114months of follow-up,no disease recurrence or death occurred.Of note,one patient who received conservative treatment successfully became pregnant.Conclusions:VGPA can be detected at an early FIGO stage with excellent prognosis.For young patients who do not exhibit poor prognosis factors,conservative treatment may be the first treatment choice based on overall assessment of clinical conditions.展开更多
基金supported by the National Key Research and Development Program of China(2023YFC2308800)the Natural Science Foundation of Shanghai(25ZR1402053)the Key Discipline of Public Health of the Shanghai Municipal Health Commission(Grant No.GWVI-11.1-07).
文摘Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus(HR-HPV)infection.Methods Female patients(n=73)with HR-HPV infection were enrolled and divided into a high-grade squamous intraepithelial lesion(HSIL)group(n=33)and a non-HSIL(N-HSIL)group(n=40),which include low-grade squamous intraepithelial lesions and inflammation.Healthy screening subjects(n=31)with negative HR-HPV results were enrolled as a control group.We examined contemporaneous plasma and secretory cytokines from 25 study subjects to investigate the difference between systemic cytokine profiles and the local microenvironment immunity using the Wilcoxon matched-pairs signed rank test.The 12 cytokines from cervical secretions were compared between the three groups using the Mann-Whitney test,and logistic regression was used to analyze HSIL and N-HSIL.Results There were statistical differences in eight cytokines(IL-2,IL-6,TNF-α,IFN-γ,IL-1β,IL-12p70,IFN-α,and IL-8)between cervical secretion and plasma of the same patient,and seven cytokines were statistically different between the control and other two groups.We selected four independent variables(TNF-α,IFN-γ,IL-12p70,and IFN-α)commonly identified by univariate regression analysis and non-parametric tests for multivariate logistic regression analysis.Based on this model,HSIL could be predicted in patients with HR-HPV infection,with the area under the curve being 0.76.Conclusion The systemic cytokine profile cannot reflect the local microenvironment immunity,and the occurrence of HSIL is related to the cytokine levels in the cervical microenvironment.
文摘Objective:Villoglandular papillary adenocarcinoma(VGPA)of the uterine cervix is a subtype of cervical adenocarcinoma.In the present study,we summarized the clinical features of VGPA of the uterine cervix and discussed the potential indications for a conservative treatment.Methods:A retrospective review of clinical characteristics and treatment aspects of 10patients with VGPA at the Obstetrics and Gynecology Hospital of Fudan University was conducted between January 2007 and December 2016.Almost all of the existing 40 English papers on“villoglandular papillary adenocarcinoma[title/abstract]”identified from PubMed were obtained.Clinical data from these papers were analyzed in terms of age,International Federation of Gynecology and Obstetrics(FIGO)stage,recurrence rate,mortality,and conservation treatment aspects.Results:The median age of 10patients with VGPA was 40years.All cases had Stage IB 1 disease.Seven patients underwent human papillomavirus examinations,which revealed 6 positive and 1 negative case(s)of infection.Six patients underwent ThinPrep cytologic tests,which revealed 4patients with atypical glandular cells,1 with a high-grade squamous intraepithelial lesion,and 1 who tested negative for intraepithelial malignancy.None of the patients had lymph node metastases.During the 6-114months of follow-up,no disease recurrence or death occurred.Of note,one patient who received conservative treatment successfully became pregnant.Conclusions:VGPA can be detected at an early FIGO stage with excellent prognosis.For young patients who do not exhibit poor prognosis factors,conservative treatment may be the first treatment choice based on overall assessment of clinical conditions.
