Objective: To evaluate the myocardial protective effect of Gualou Xiebai Banxia decoction (瓜蒌薤白半夏汤GXBD) and explore the mechanisms of inhibition of NF-kappa B activation and blockade of inflammatory responses i...Objective: To evaluate the myocardial protective effect of Gualou Xiebai Banxia decoction (瓜蒌薤白半夏汤GXBD) and explore the mechanisms of inhibition of NF-kappa B activation and blockade of inflammatory responses induced by ischemia-reperfusion in rats. Methods: Twenty-four Sprague Dawley (SD) rats were randomly divided into three groups. Rats in the treatment group received GXBD (13 g crude drug/kg) for three weeks, while rats in the model control and normal control groups received equal volumes of distilled water. On the 22nd day, rats in the ischemia-reperfusion (I/R) control and GXBD-treated groups underwent 30 min occlusion of the left anterior descending (LAD) coronary artery, followed by 120 min reperfusion. Electrocardiogram was recorded, and the activities of cardiac enzymes, cytokines, and NF-кB were assessed after I/R. Results: Compared with the I/R control group, GXBD treatment restored the activity of the specific myocardial-injury marker creatine kinase (CK) and lactate dehydrogenase (LDH), and inhibited the inflammatory response involving the nuclear factor-кB (NF-кB) pathway, including down-regulation of interleukin (IL)-1β and IL-6, and up-regulation of IL-10 gene expression. Conclusion: GXBD strongly reduced myocardial impairment in our I/R model, including inhibition of NF-кB activation and inflammatory cytokine responses.展开更多
Objective： To investigate the preventive effects of Qiangzhi Decoction （羌跖汤, QZD） on influenza A pneumonia through inhibition of inflammatory cytokine storm in vivo and in vitro. Methods： One hundred ICR mice w...Objective： To investigate the preventive effects of Qiangzhi Decoction （羌跖汤, QZD） on influenza A pneumonia through inhibition of inflammatory cytokine storm in vivo and in vitro. Methods： One hundred ICR mice were randomly divided into the virus control, the Tamiflu control and the QZD high-, medium-, and low-dose groups. Mice were infected intranasally with influenza virus （H1N1） at 10 median lethal dose （LDso）. QZD and Tamiflu were administered intragastrically twice daily from day 0 to day 7 after infection. The virus control group was treated with distilled water alone under the same condition. The number of surviving mice was recorded daily for 14 days after viral infection. The histological damage and viral replication and the expression of inflammatory cytokines were monitored. Additionally, the suppression capacity on the secretion of regulated on activation normal T cells expressed and secreted （RANTES） and tumor necrosis factor-α （TNF-α ） in epithelial and macrophage cell-lines were evaluated. Results： Compared with the virus control group, the survival rate of the QZD groups significantly improved in a dose-dependent manner （P〈0.05）, the viral titers in lung tissue was inhibited （P〈0.05）, and the production of inflammatory cytokines interferon-γ （IFN- α ）, interleukin-6 （IL-6）, TNF-α, and intercellular adhesion molecule-1 （ICAM-1） were suppressed （P〈0.05）. Meanwhile, the secretion of RANTETS and TNF-α by epithelial and macrophage cell-lines was inhibited with the treatment of QZD respectively in vitro （P〈0.05）. Conclusions： The preventive effects of QZD on influenza virus infection might be due to its unique cytokine inhibition mechanism. QZD may have significant therapeutic potential in combination with antiviral drugs.展开更多
Objective:Nonsmall-cell lung cancer(NSCLC)is an aggressive,highly chemoresistant disease.Taxol is an effective chemotherapeutic drug widely used for the treatment of NSCLC.However,the clinical use of Taxol is limited ...Objective:Nonsmall-cell lung cancer(NSCLC)is an aggressive,highly chemoresistant disease.Taxol is an effective chemotherapeutic drug widely used for the treatment of NSCLC.However,the clinical use of Taxol is limited due to the occurrence of adverse side effects under high therapeutic doses.Therefore,it is desirable to explore combination therapy to reduce the dose of chemotherapeutic drugs and achieve excellent outcomes.A biosynthetic ginsenoside,3-O-β-D-glucopyranosyl-dammar-24-ene-3β,20 S-diol(3β-O-Glc-DM,C3 DM)is obtained from microbial fermentation by metabolic engineering.Based on previous study findings,we aimed to explore the mechanism of combination therapy with C3 DM and Taxol and its increasing antitumor effect on Lewis lung cancer(LLC)in this study.Materials and Methods:A thiazolyl blue tetrazolium bromide(MTT)assay was performed to evaluate cell viability;the apoptotic effect was studied using cell apoptosis assay.The Lewis tumor xenograft experiment was performed to determine the effects of C3 DM combined with Taxol on tumor growth in vivo,and western blotting was performed to analyze protein expressions.Results:C3 DM effectively inhibited the proliferation of NSCLC cells.Moreover,C3 DM increased the antiproliferative activity of Taxol and significantly enhanced cell apoptosis induced by Taxol in Lewis lung cancer cells.C3 DM alone also suppressed Lewis tumor growth and enhanced the antitumor activity of Taxol in vivo.Western blot analysis revealed that the effects of the antiproliferation and apoptosis induction of C3 DM treatment alone or in combination with Taxol on Lewis lung cancer were mediated by inhibiting the interleukin-6(IL-6)/Jak2/STAT3 and IL-6/AKT signaling pathways.Conclusions:The results showed that C3 DM has the potential to be used in combination therapy with Taxol against NSCLC.展开更多
基金supported by the National Natural Science Foundation of China (30701066 and 30973696 Science) for financial support
文摘Objective: To evaluate the myocardial protective effect of Gualou Xiebai Banxia decoction (瓜蒌薤白半夏汤GXBD) and explore the mechanisms of inhibition of NF-kappa B activation and blockade of inflammatory responses induced by ischemia-reperfusion in rats. Methods: Twenty-four Sprague Dawley (SD) rats were randomly divided into three groups. Rats in the treatment group received GXBD (13 g crude drug/kg) for three weeks, while rats in the model control and normal control groups received equal volumes of distilled water. On the 22nd day, rats in the ischemia-reperfusion (I/R) control and GXBD-treated groups underwent 30 min occlusion of the left anterior descending (LAD) coronary artery, followed by 120 min reperfusion. Electrocardiogram was recorded, and the activities of cardiac enzymes, cytokines, and NF-кB were assessed after I/R. Results: Compared with the I/R control group, GXBD treatment restored the activity of the specific myocardial-injury marker creatine kinase (CK) and lactate dehydrogenase (LDH), and inhibited the inflammatory response involving the nuclear factor-кB (NF-кB) pathway, including down-regulation of interleukin (IL)-1β and IL-6, and up-regulation of IL-10 gene expression. Conclusion: GXBD strongly reduced myocardial impairment in our I/R model, including inhibition of NF-кB activation and inflammatory cytokine responses.
基金Supported by the National Natural Science Foundation of China(NO.81173173)Shanghai Yan De-xin Foundation for Traditional Chinese Medicine
文摘Objective： To investigate the preventive effects of Qiangzhi Decoction （羌跖汤, QZD） on influenza A pneumonia through inhibition of inflammatory cytokine storm in vivo and in vitro. Methods： One hundred ICR mice were randomly divided into the virus control, the Tamiflu control and the QZD high-, medium-, and low-dose groups. Mice were infected intranasally with influenza virus （H1N1） at 10 median lethal dose （LDso）. QZD and Tamiflu were administered intragastrically twice daily from day 0 to day 7 after infection. The virus control group was treated with distilled water alone under the same condition. The number of surviving mice was recorded daily for 14 days after viral infection. The histological damage and viral replication and the expression of inflammatory cytokines were monitored. Additionally, the suppression capacity on the secretion of regulated on activation normal T cells expressed and secreted （RANTES） and tumor necrosis factor-α （TNF-α ） in epithelial and macrophage cell-lines were evaluated. Results： Compared with the virus control group, the survival rate of the QZD groups significantly improved in a dose-dependent manner （P〈0.05）, the viral titers in lung tissue was inhibited （P〈0.05）, and the production of inflammatory cytokines interferon-γ （IFN- α ）, interleukin-6 （IL-6）, TNF-α, and intercellular adhesion molecule-1 （ICAM-1） were suppressed （P〈0.05）. Meanwhile, the secretion of RANTETS and TNF-α by epithelial and macrophage cell-lines was inhibited with the treatment of QZD respectively in vitro （P〈0.05）. Conclusions： The preventive effects of QZD on influenza virus infection might be due to its unique cytokine inhibition mechanism. QZD may have significant therapeutic potential in combination with antiviral drugs.
基金supported by the Chinese Academy of Medical Sciences(CAMS)Initiation fund for Medical Science(2016-I2M-1-008)the National Natural Science Foundation of China(Approval no.81673341)
文摘Objective:Nonsmall-cell lung cancer(NSCLC)is an aggressive,highly chemoresistant disease.Taxol is an effective chemotherapeutic drug widely used for the treatment of NSCLC.However,the clinical use of Taxol is limited due to the occurrence of adverse side effects under high therapeutic doses.Therefore,it is desirable to explore combination therapy to reduce the dose of chemotherapeutic drugs and achieve excellent outcomes.A biosynthetic ginsenoside,3-O-β-D-glucopyranosyl-dammar-24-ene-3β,20 S-diol(3β-O-Glc-DM,C3 DM)is obtained from microbial fermentation by metabolic engineering.Based on previous study findings,we aimed to explore the mechanism of combination therapy with C3 DM and Taxol and its increasing antitumor effect on Lewis lung cancer(LLC)in this study.Materials and Methods:A thiazolyl blue tetrazolium bromide(MTT)assay was performed to evaluate cell viability;the apoptotic effect was studied using cell apoptosis assay.The Lewis tumor xenograft experiment was performed to determine the effects of C3 DM combined with Taxol on tumor growth in vivo,and western blotting was performed to analyze protein expressions.Results:C3 DM effectively inhibited the proliferation of NSCLC cells.Moreover,C3 DM increased the antiproliferative activity of Taxol and significantly enhanced cell apoptosis induced by Taxol in Lewis lung cancer cells.C3 DM alone also suppressed Lewis tumor growth and enhanced the antitumor activity of Taxol in vivo.Western blot analysis revealed that the effects of the antiproliferation and apoptosis induction of C3 DM treatment alone or in combination with Taxol on Lewis lung cancer were mediated by inhibiting the interleukin-6(IL-6)/Jak2/STAT3 and IL-6/AKT signaling pathways.Conclusions:The results showed that C3 DM has the potential to be used in combination therapy with Taxol against NSCLC.
