Species of the genus Paenibacillus,especially those from extreme environments that have been reported,are known for producing bioactive compounds with agricultural and biotechnological applications.In this study,we in...Species of the genus Paenibacillus,especially those from extreme environments that have been reported,are known for producing bioactive compounds with agricultural and biotechnological applications.In this study,we investigated the genomic and biochemical potential of Paenibacillus sp.VCA1 strain isolated from a thermophilic environment.Taxonomic identification was performed using whole genome similarity analysis,TETRA four-nucleotide frequency of occurrence analysis,ANI average nucleotide identity analysis,and gene distance analysis using digital DNA-DNA hybridization(dDDH).Functional analysis of the strain VCA1 was performed by detecting genes,enzymes,and genome subsystems involved in biocontrol and plant growth promotion,which was carried out using the RAST(Rapid Annotation using Subsystem Technology),the seed server and antiSAMSH(antibiotics&Secondary Metabolite Analysis Shell).Genetic analyses showed the existence of 11 fatty acid and isoprenoid production genes,56 motility and chemotaxis genes,29 N-acetylglucosamine genes,and five siderophore genes.Finally,the antifungal and emulsifying activities demonstrated that strain VCA1 has activity against Fusarium oxysporum strain 45ta using integrated genomic and experimental validation,and the antifungal properties of the Paenibacillus sp.VCA1 has suggested potential use as a biocontrol agent against phytopathogenic fungi,and its continuous study can have beneficial applications in sustainable agriculture and biotechnology.展开更多
Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation,...Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed.展开更多
Paravalvular Leakage(PVL)has been recognized as one of the most dangerous complications in relation to Transcathether Aortic Valve Implantation(TAVI)activities.However,data available in the literature about Fluid Stru...Paravalvular Leakage(PVL)has been recognized as one of the most dangerous complications in relation to Transcathether Aortic Valve Implantation(TAVI)activities.However,data available in the literature about Fluid Structure Interaction(FSI)for this specific problem are relatively limited.In the present study,the fluid and structure responses of the hemodynamics along the patient aorta model and the aortic wall deformation are studied with the aid of numerical simulation taking into account PVL and 100%TAVI valve opening.In particular,the aorta without valve(AWoV)is assumed as the normal condition,whereas an aorta with TAVI 26 mm for 100%Geometrical Orifice Area(GOA)is considered as the patient aorta with PVL complication.A 3D patient-specific aorta model is elaborated using the MIMICS software.Implantation of the identical TAVI valve of Edward SAPIEN XT 26(Edwards Lifes ciences,Irvine,California)is considered.An undersized 26 mm TAVI valve with 100%valve opening is selected to mimic the presence of PVL at the aortic annulus.The present research indicates that the existence of PVL can increase the blood velocity,pressure drop and WSS in comparison to normal conditions,thereby paving the way to the development of recirculation flow,thrombus formation,aorta wall collapse,aortic rupture and damage of endothelium.展开更多
Strategic materials design is essential for the development of small-diameter,tissue-engineered vascular grafts.Self-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as t...Strategic materials design is essential for the development of small-diameter,tissue-engineered vascular grafts.Self-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as they replicate the organized elastin structure of native blood vessels.Further,the bioactivity of nanofibers can be modified by the addition of functional peptide motifs.In the present study,we describe the development of a novel nanofiber-forming elastin-like polypeptide(ELP)with an arginine-glutamic acid-aspartic acid-valine(REDV)sequence.The biological characteristics of the REDV-modified ELP nanofibers relevant to applications in vascular grafting were compared to ELP without ligands for integrin,ELP with arginine-glycine-aspartic acid(RGD)sequence,collagen and cell culture glass.Among them,REDV-modified ELP nanofibers met the preferred biological properties for vascular graft materials,i.e.(i)inhibition of platelet adhesion and activation,(ii)endothelial cell adhesion and proliferation and(iii)maintenance of smooth muscle cells in a contractile phenotype to prevent cell overgrowth.The results indicate that REDV-modified ELP nanofibers represent promising candidates for the further development of small-diameter vascular grafts.展开更多
Background:Conventional drying using heated air oven is commonly used as a method for preserving the product but often affects the nutritional value,taste,and texture.However,the heat from the drying method can oxidiz...Background:Conventional drying using heated air oven is commonly used as a method for preserving the product but often affects the nutritional value,taste,and texture.However,the heat from the drying method can oxidize and destroy heat-sensitive compounds.Superheated steam(SHS)drying uses superheated steam instead of hot air or combustion gases in a direct dryer and was reported better at preserving the nutritional values of food products.Aim:To evaluate the effect of SHS drying on antioxidant properties of tea leaves.The study also compared SHS drying with conventional and freeze-drying methods.Results:Tea leaves dried using freeze drying retained the highest level of antioxidant properties compared to other drying methods.The leaves dried using SHS exhibited significantly higher radical scavenging activity,ORAC and FRAP values compared to oven drying method.At different drying temperatures(150℃and 175℃),oven dried leaves showed significantly higher(p<0.05)antioxidant properties than that of SHS dried ones.Tea leaves dried for 60,75,and 90 min using SHS showed significantly higher(p<0.05)FRAP and ORAC values,and also total phenolic content compared to oven dried tea leaves.Conclusion:Tea leaves dried using SHS drying method retained higher level of antioxidant properties compared to oven drying.The drying method also retained lower antioxidant properties as drying time increased.Further study involving SHS drying in food-related fields should be conducted to support its usefulness.展开更多
基金funded by Tecnologico Nacional de Mexico/IT de Tuxtla Gutierrez with project number 22847.25-PFunding came from the“Consejo Nacional de Humanidades,Ciencias y Tecnologias”(CONAHCYT,Mexico),with Project No.320299.
文摘Species of the genus Paenibacillus,especially those from extreme environments that have been reported,are known for producing bioactive compounds with agricultural and biotechnological applications.In this study,we investigated the genomic and biochemical potential of Paenibacillus sp.VCA1 strain isolated from a thermophilic environment.Taxonomic identification was performed using whole genome similarity analysis,TETRA four-nucleotide frequency of occurrence analysis,ANI average nucleotide identity analysis,and gene distance analysis using digital DNA-DNA hybridization(dDDH).Functional analysis of the strain VCA1 was performed by detecting genes,enzymes,and genome subsystems involved in biocontrol and plant growth promotion,which was carried out using the RAST(Rapid Annotation using Subsystem Technology),the seed server and antiSAMSH(antibiotics&Secondary Metabolite Analysis Shell).Genetic analyses showed the existence of 11 fatty acid and isoprenoid production genes,56 motility and chemotaxis genes,29 N-acetylglucosamine genes,and five siderophore genes.Finally,the antifungal and emulsifying activities demonstrated that strain VCA1 has activity against Fusarium oxysporum strain 45ta using integrated genomic and experimental validation,and the antifungal properties of the Paenibacillus sp.VCA1 has suggested potential use as a biocontrol agent against phytopathogenic fungi,and its continuous study can have beneficial applications in sustainable agriculture and biotechnology.
基金Supported by JSPS grant Challenging Exploratory Research,No.25660062SEP-CONACyT grant,No.152794(García-Contreras R)+3 种基金Fideicomiso COLPOS 167304 and Programa Cátedras-CONACyT 2112(Castillo-Juárez)the Miguel Servet Program(C.H.U.A Coruña and ISCIII)(Tomás M)the CONACyT grant number 441393/269132(Mandujano-Tinoco EA)and the Biotechnology Endowed Chair at the Pennsylvania State University.
文摘Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed.
基金Universiti Putra Malaysia,for providing funds for this project through Grant UPM GP-IPM/2019/9675000.
文摘Paravalvular Leakage(PVL)has been recognized as one of the most dangerous complications in relation to Transcathether Aortic Valve Implantation(TAVI)activities.However,data available in the literature about Fluid Structure Interaction(FSI)for this specific problem are relatively limited.In the present study,the fluid and structure responses of the hemodynamics along the patient aorta model and the aortic wall deformation are studied with the aid of numerical simulation taking into account PVL and 100%TAVI valve opening.In particular,the aorta without valve(AWoV)is assumed as the normal condition,whereas an aorta with TAVI 26 mm for 100%Geometrical Orifice Area(GOA)is considered as the patient aorta with PVL complication.A 3D patient-specific aorta model is elaborated using the MIMICS software.Implantation of the identical TAVI valve of Edward SAPIEN XT 26(Edwards Lifes ciences,Irvine,California)is considered.An undersized 26 mm TAVI valve with 100%valve opening is selected to mimic the presence of PVL at the aortic annulus.The present research indicates that the existence of PVL can increase the blood velocity,pressure drop and WSS in comparison to normal conditions,thereby paving the way to the development of recirculation flow,thrombus formation,aorta wall collapse,aortic rupture and damage of endothelium.
基金supported by the Ministry of Education,Culture,Sports,Science and Technology(MEXT)Program:Data Creation and Utilization-Type Material Research and Development Project[JPMXP1122714694]Japan Society for the Promotion of Science(JSPS)KAKENHI[19H03747]+1 种基金Japan Agency for Medical Research and Development(AMED)[JP20lm0203005]a Research Grant from Japan Association for Chemical Innovation(JACI).
文摘Strategic materials design is essential for the development of small-diameter,tissue-engineered vascular grafts.Self-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as they replicate the organized elastin structure of native blood vessels.Further,the bioactivity of nanofibers can be modified by the addition of functional peptide motifs.In the present study,we describe the development of a novel nanofiber-forming elastin-like polypeptide(ELP)with an arginine-glutamic acid-aspartic acid-valine(REDV)sequence.The biological characteristics of the REDV-modified ELP nanofibers relevant to applications in vascular grafting were compared to ELP without ligands for integrin,ELP with arginine-glycine-aspartic acid(RGD)sequence,collagen and cell culture glass.Among them,REDV-modified ELP nanofibers met the preferred biological properties for vascular graft materials,i.e.(i)inhibition of platelet adhesion and activation,(ii)endothelial cell adhesion and proliferation and(iii)maintenance of smooth muscle cells in a contractile phenotype to prevent cell overgrowth.The results indicate that REDV-modified ELP nanofibers represent promising candidates for the further development of small-diameter vascular grafts.
基金supported by the School of Graduate Studies and the Department of Nutrition and Dietetics of Universiti Putra Malaysia(Malaysia),in collaboration with the Graduate School of Life Science and System Engineering of Kyushu Institute of Technology(Japan).
文摘Background:Conventional drying using heated air oven is commonly used as a method for preserving the product but often affects the nutritional value,taste,and texture.However,the heat from the drying method can oxidize and destroy heat-sensitive compounds.Superheated steam(SHS)drying uses superheated steam instead of hot air or combustion gases in a direct dryer and was reported better at preserving the nutritional values of food products.Aim:To evaluate the effect of SHS drying on antioxidant properties of tea leaves.The study also compared SHS drying with conventional and freeze-drying methods.Results:Tea leaves dried using freeze drying retained the highest level of antioxidant properties compared to other drying methods.The leaves dried using SHS exhibited significantly higher radical scavenging activity,ORAC and FRAP values compared to oven drying method.At different drying temperatures(150℃and 175℃),oven dried leaves showed significantly higher(p<0.05)antioxidant properties than that of SHS dried ones.Tea leaves dried for 60,75,and 90 min using SHS showed significantly higher(p<0.05)FRAP and ORAC values,and also total phenolic content compared to oven dried tea leaves.Conclusion:Tea leaves dried using SHS drying method retained higher level of antioxidant properties compared to oven drying.The drying method also retained lower antioxidant properties as drying time increased.Further study involving SHS drying in food-related fields should be conducted to support its usefulness.
