A comparative analysis was conducted on seventeen sprouts from vegetable and non-vegetable sources belonging to various plant families such as Asteraceae,Brassicaceae,Fabaceae,and Poaceae.The study focused on evaluati...A comparative analysis was conducted on seventeen sprouts from vegetable and non-vegetable sources belonging to various plant families such as Asteraceae,Brassicaceae,Fabaceae,and Poaceae.The study focused on evaluating their phytochemical composition(total phenolics,glucosinolates,and anthocyanins),antioxidant capacity(DPPH assay),antidiabetic activity(inhibition of α-glucosidase and α-amylase),and hypocholesterolemic effect(inhibition of HMGR).Multivariate statistical analysis indicated that vegetable sprouts generally exhibited higher levels of phytochemicals and bioactivities compared to cereal sprouts.Red radish sprouts,in particular,showed the highest concentrations of all analyzed phytochemicals and significant bioactivities.Principal component analysis and Pearson correlation analysis underscored the importance of antioxidants,which subsequently affect antidiabetic and hypocholesterolemic activities.Among the phytochemicals,phenolic compounds and glucosinolates were highlighted as more significant than anthocyanins,considering their types and synergistic effects.Molecular docking analysis suggested that these compounds possess strong affinity and specificity towards the enzymes mentioned,indicating their potential for innovative therapeutic interventions aimed at modulating enzyme activity.展开更多
In this study,we examined the mechanisms by which different developmental stages affect the phytochemical compositions andα-glucosidase inhibitory(AGI)activities of three non-pungent pepper cultivars(MI,DJ,and KS).Ea...In this study,we examined the mechanisms by which different developmental stages affect the phytochemical compositions andα-glucosidase inhibitory(AGI)activities of three non-pungent pepper cultivars(MI,DJ,and KS).Early stages,particularly the green fruit stage,were crucial for high AGI activity and total phenolic and flavonoid contents.During fruit maturation,carotenoid accumulation negatively affected the AGI activity in a cultivar-dependent manner.Capsaicinoid content varied among the cultivars,possibly affecting the AGI activity.Molecular docking analysis revealed the strong binding affinities of phenolic and flavonoid compounds forα-glucosidase,which were correlated with enhanced enzyme inhibition.Although strong correlations were observed between AGI activity and total phenolic and flavonoid contents,variations in carotenoid and capsaicinoid contents affected the AGI activity differently across all cultivars.Collectively,our findings highlight the importance of harvesting peppers at the right stage to optimize their nutritional and medicinal benefits,providing valuable insights for the development of effective breeding strategies to enhance their anti-diabetic potential.展开更多
This study investigated the maturation-dependentα-glucosidase inhibitory(AGI)potential of metabolites in two nonpungent Capsicum annuum cultivars,“Meein”and“Dangjo,”using an integrated approach that combined meta...This study investigated the maturation-dependentα-glucosidase inhibitory(AGI)potential of metabolites in two nonpungent Capsicum annuum cultivars,“Meein”and“Dangjo,”using an integrated approach that combined metabolomics,molecular docking,and in vitro enzyme assays.UPLC-Orbitrap-MS-based metabolite profiling of fruits at different ripening stages revealed distinct metabolite profiles with varying AGI activities.Multivariate statistical analyses,including partial least squares regression and Pearson's correlation,identified 37 metabolites,particularly phenolic compounds,that were significantly correlated with AGI activity(P<0.05,VIP>1.5);several have limited prior evidence directly linking them toα-glucosidase inhibition.Molecular docking simu-lations predicted strong binding affinities between these metabolites andα-glucosidase,primarily driven by hydrogen bonding and van der Waals interactions.These predictions were supported by in vitro AGI activity assays using pure metabolite standards,in which caffeic acid showed the strongest inhibitory activity(IC_(50)=10.86μmol/L),outperforming the reference inhibitor acarbose(IC_(50)=17.26μmol/L).Sinapinic and 3-hydroxy-butyric acids also demonstrated significant AGI potential,with their combined use further enhancing the inhibitory effect(IC_(50)=6.98μmol/L).Lineweaver-Burk kinetic analysis revealed distinct modes of inhibition:competitive inhibition by 3-hydroxybutyric acid;noncompetitive inhibition by 3-hydroxybenzoic acid and 4,7-dihydroxycoumarin;and mixed-type inhibition by caffeic and sinapinic acids.In silico secondary structure analysis further confirmed the specificity of the ligand-enzyme interactions.Overall,these findings highlight the potential of naturally occurring C.annuum phenolic metabolites as powerfulα-glucosidase inhibitors and suggest they could serve as natural alternatives to synthetic antidiabetic drugs for managing postprandial hyperglycemia.展开更多
基金supported by the Cooperative Research Program for Agriculture Science and Technology Development(RS-2024-00322321)of the Rural Development Administration(RDA)of Korea.
文摘A comparative analysis was conducted on seventeen sprouts from vegetable and non-vegetable sources belonging to various plant families such as Asteraceae,Brassicaceae,Fabaceae,and Poaceae.The study focused on evaluating their phytochemical composition(total phenolics,glucosinolates,and anthocyanins),antioxidant capacity(DPPH assay),antidiabetic activity(inhibition of α-glucosidase and α-amylase),and hypocholesterolemic effect(inhibition of HMGR).Multivariate statistical analysis indicated that vegetable sprouts generally exhibited higher levels of phytochemicals and bioactivities compared to cereal sprouts.Red radish sprouts,in particular,showed the highest concentrations of all analyzed phytochemicals and significant bioactivities.Principal component analysis and Pearson correlation analysis underscored the importance of antioxidants,which subsequently affect antidiabetic and hypocholesterolemic activities.Among the phytochemicals,phenolic compounds and glucosinolates were highlighted as more significant than anthocyanins,considering their types and synergistic effects.Molecular docking analysis suggested that these compounds possess strong affinity and specificity towards the enzymes mentioned,indicating their potential for innovative therapeutic interventions aimed at modulating enzyme activity.
基金funded by the Ministry of Agriculture,Food,and Rural Affairs(MAFRA,RS-2022-IP3220681)through the IPET Digital Breeding Technology Development Programthe Basic Science Research Program(RS-2023-00208020),administered by the National Research Foundation of Korea.
文摘In this study,we examined the mechanisms by which different developmental stages affect the phytochemical compositions andα-glucosidase inhibitory(AGI)activities of three non-pungent pepper cultivars(MI,DJ,and KS).Early stages,particularly the green fruit stage,were crucial for high AGI activity and total phenolic and flavonoid contents.During fruit maturation,carotenoid accumulation negatively affected the AGI activity in a cultivar-dependent manner.Capsaicinoid content varied among the cultivars,possibly affecting the AGI activity.Molecular docking analysis revealed the strong binding affinities of phenolic and flavonoid compounds forα-glucosidase,which were correlated with enhanced enzyme inhibition.Although strong correlations were observed between AGI activity and total phenolic and flavonoid contents,variations in carotenoid and capsaicinoid contents affected the AGI activity differently across all cultivars.Collectively,our findings highlight the importance of harvesting peppers at the right stage to optimize their nutritional and medicinal benefits,providing valuable insights for the development of effective breeding strategies to enhance their anti-diabetic potential.
基金supported by the Korea Basic Science Institute(National Research Facilities and Equipment Center)grant funded by the Ministry of Education(grant no.RS-2023-NF001356)the Regional Innovation System&Education(RISE)through theSeoul RISE Center,funded by the Ministry of Education(MOE)and the Seoul Metropolitan Government(2025-RISE-01-019-04)+3 种基金the Cooperative Research Pro-gram for Agricultural Science and Technology Development(RS-2024-00322321)of the Rural Development Administration(RDA)the Basic Science Research Program(RS-2023-00208020)the National Research Foundation of Koreaand the Sejong University Sabbatical Research Program.
文摘This study investigated the maturation-dependentα-glucosidase inhibitory(AGI)potential of metabolites in two nonpungent Capsicum annuum cultivars,“Meein”and“Dangjo,”using an integrated approach that combined metabolomics,molecular docking,and in vitro enzyme assays.UPLC-Orbitrap-MS-based metabolite profiling of fruits at different ripening stages revealed distinct metabolite profiles with varying AGI activities.Multivariate statistical analyses,including partial least squares regression and Pearson's correlation,identified 37 metabolites,particularly phenolic compounds,that were significantly correlated with AGI activity(P<0.05,VIP>1.5);several have limited prior evidence directly linking them toα-glucosidase inhibition.Molecular docking simu-lations predicted strong binding affinities between these metabolites andα-glucosidase,primarily driven by hydrogen bonding and van der Waals interactions.These predictions were supported by in vitro AGI activity assays using pure metabolite standards,in which caffeic acid showed the strongest inhibitory activity(IC_(50)=10.86μmol/L),outperforming the reference inhibitor acarbose(IC_(50)=17.26μmol/L).Sinapinic and 3-hydroxy-butyric acids also demonstrated significant AGI potential,with their combined use further enhancing the inhibitory effect(IC_(50)=6.98μmol/L).Lineweaver-Burk kinetic analysis revealed distinct modes of inhibition:competitive inhibition by 3-hydroxybutyric acid;noncompetitive inhibition by 3-hydroxybenzoic acid and 4,7-dihydroxycoumarin;and mixed-type inhibition by caffeic and sinapinic acids.In silico secondary structure analysis further confirmed the specificity of the ligand-enzyme interactions.Overall,these findings highlight the potential of naturally occurring C.annuum phenolic metabolites as powerfulα-glucosidase inhibitors and suggest they could serve as natural alternatives to synthetic antidiabetic drugs for managing postprandial hyperglycemia.
