Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cellsinduced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells fromhealthy adults.Me...Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cellsinduced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells fromhealthy adults.Methods We used conventional methods to induce CIK cells originating from two peripheral bloodmononuclear cell types (from patients with breast cancer and healthy adults). Killing activity was detectedusing an LDH assay, immunophenotypic changes were analyzed by flow cytometry, and the IFN-γ level ofculture supernatants was detected by ELISA.Results The results showed that the proliferative capacity of the allogeneic CIK cells was significantlyhigher than that of the autologous CIK cells. Compared with autologous CIK cells, the allogeneic CIK cellshad significantly enhanced anti-tumor activity against SKBR-3 cells (P < 0.01) and IFN-γ secretion (P <0.05);moreover, they increased the ratio of CD3+ CD56+ cells and CD3+ CD8+ cells (P < 0.05).Conclusion Healthy adult-derived induced CIK cells exhibited a stronger anti-tumor effect than inducedCIK cells derived from patients with breast cancer. The results of this study could provide experimentalevidence for the clinical application of CIK cells.展开更多
Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes(HSP70-PCs)on HER-3-overexpressing breast cancer.Methods In this study,we first s...Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes(HSP70-PCs)on HER-3-overexpressing breast cancer.Methods In this study,we first studied the expression of HER-3 in breast cancer tissues and its relationship with patient characteristics.We then purified HSP70-PCs from primary breast cancer cells with different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic cells(DCs)and CD8+T cells induced by these complexes.Third,recombinant human HSP70-HER-3 protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3-overexpressing breast cancer cells,and the resulting immunological response was examined.Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these treatments induce autogenous CD8+T cell killing of HER-3-positive breast cancer cells.Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients with HER-3-overexpressing breast cancer.展开更多
基金Supported by a grant from the National Natural Sciences Foundation of Inner Mongolia(No.2012MS1102).
文摘Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cellsinduced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells fromhealthy adults.Methods We used conventional methods to induce CIK cells originating from two peripheral bloodmononuclear cell types (from patients with breast cancer and healthy adults). Killing activity was detectedusing an LDH assay, immunophenotypic changes were analyzed by flow cytometry, and the IFN-γ level ofculture supernatants was detected by ELISA.Results The results showed that the proliferative capacity of the allogeneic CIK cells was significantlyhigher than that of the autologous CIK cells. Compared with autologous CIK cells, the allogeneic CIK cellshad significantly enhanced anti-tumor activity against SKBR-3 cells (P < 0.01) and IFN-γ secretion (P <0.05);moreover, they increased the ratio of CD3+ CD56+ cells and CD3+ CD8+ cells (P < 0.05).Conclusion Healthy adult-derived induced CIK cells exhibited a stronger anti-tumor effect than inducedCIK cells derived from patients with breast cancer. The results of this study could provide experimentalevidence for the clinical application of CIK cells.
基金Supported by a grant from the National Natural Science Foundation of China(No.81260392).
文摘Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes(HSP70-PCs)on HER-3-overexpressing breast cancer.Methods In this study,we first studied the expression of HER-3 in breast cancer tissues and its relationship with patient characteristics.We then purified HSP70-PCs from primary breast cancer cells with different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic cells(DCs)and CD8+T cells induced by these complexes.Third,recombinant human HSP70-HER-3 protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3-overexpressing breast cancer cells,and the resulting immunological response was examined.Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these treatments induce autogenous CD8+T cell killing of HER-3-positive breast cancer cells.Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients with HER-3-overexpressing breast cancer.
